MG1655
W3110
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Gene Report : thiD
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion 16-1-2 (LD)  ,  DE(sbcB-rfb)202  ,  OCL74-2 (D)  ,  OCL74-2,75-5 (LD 16-1-2_CRS) (D)  ,  OCL74-2,75-6 (LD 16-1-2_P/O) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  thiD
Related strains
     Strains Search Search strains by gene name:  thiD   Search strains by all related name:  thiD b2103 ECK2096 f266 f266c JW2090 thiA thiJ thiN

General information  (Go to Linear View:)
 Gene Name thiD  
 Alternative name b2103,ECK2096,f266,f266c,JW2090,thiA,thiJ,thiN  
 Location, Length 2,181,738 - 2,182,538 (  -  ) ;   47.02 min ; 801 (bp) ,   266 (aa) Go to Linear View
 Product bifunctional hydroxy-methylpyrimidine kinase/ hydroxy-phosphomethylpyrimidine kinase  
 Operon Name thiMD  
 Note phosphomethylpyrimidine kinase; GO_process: GO:0009228 - thiamin biosynthetic process  
 Function enzyme; Biosynthesis of cofactors, carriers: Thiamin  
 Gene Ontology GO:0008972 ; phosphomethylpyrimidine kinase activity ( thiD )
GO:0009228 ; thiamin biosynthetic process ( thiD )
GO:0016301 ; kinase activity ( thiD )
GO:0016740 ; transferase activity ( thiD )
 PID 1788420  
 EC number
  (KEGG Pathway)
 
2.7.1.49   2.7.4.7  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) THID_ECOLI ( P76422 )
    -  Protein name Phosphomethylpyrimidine kinase  
    -  Synonyms EC 2.7.4.7, HMP-phosphate kinase, HMP-P kinase  
    -  Gene name Name=thiD; OrderedLocusNames=b2103;  

 Linear View (Whole Mode)
View Location
   2170.0  –  2195.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP thiD (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, 4-Amino-5-Hydroxymethyl-2-Methylpyrimidine Phosphate Kinase From Salmonella Typhimurium  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Phosphomethylpyrimidine kinase  
         PSI-BLAST Pyridoxamine kinase
    nr     (database updated : 2007.02.20 )
         BLAST phosphomethylpyrimidine kinase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG0351H 
    EcoCyc thiD 

MMBR References
Experientia. 1981;37(12):1265-6.
thiD locus of Escherichia coli.
Imamura N, Nakayama H. ( 6276219 )

Sequences
Amino acid
FASTA format
0001 MKRINALTIA GTDPSGGAGI QADLKTFSAL GAYGCSVITA LVAQNTRGVQ SVYRIEPDFV AAQLDSVFSD 
0071 VRIDTTKIGM LAETDIVEAV AERLQRYQIQ NVVLDTVMLA KSGDPLLSPS AVATLRSRLL PQVSLITPNL 
0141 PEAAALLDAP HARTEQEMLE QGRSLLAMGC GAVLMKGGHL DDEQSPDWLF TREGEQRFTA PRIMTKNTHG 
0211 TGCTLSAALA ALRPRHTNWA DTVQEAKSWL SSALAQADTL EVGHGIGPVH HFHAWW

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             aacaagccgg agaacgcgca gtcgccagaa 
-070 gcgaggggcc aggcagtttt gttccacatt tccttgatgc gctctggcaa ttgacgcagg aggtgcaggc 
0001 atgaaacgaa ttaacgctct gacgattgcc ggtactgatc cgagtggtgg tgcggggatt caggccgatc 
0071 ttaaaacctt ctcggcactt ggcgcttatg gttgctcagt tattactgca ctggtggcgc aaaatacccg 
0141 tggcgtacag tcggtgtatc gcattgagcc tgattttgtc gccgcccagc tcgattcggt gttcagcgat 
0211 gtgcgaatcg ataccactaa aatcggtatg ctggcggaaa ccgatattgt tgaagcggtg gcagaacggt 
0281 tgcaacgtta tcagatccaa aacgtggtac tcgacaccgt tatgctggca aaaagcggcg acccgctgct 
0351 ttcaccttcg gcggttgcta cgctgcgcag tcgattattg ccacaggttt cattaataac gccaaacttg 
0421 cccgaagctg ccgccttgct cgacgcgcca cacgcgcgca ccgaacagga aatgctggaa caagggcgat 
0491 cgctgttggc gatgggctgt ggcgcagtgc taatgaaagg tggtcatctg gatgatgagc aaagcccgga 
0561 ctggctgttt acccgcgagg gtgaacaacg gtttaccgca ccgcgcatta tgaccaaaaa cacccacggc 
0631 actggttgta cactctctgc ggcgttggct gcactacgcc cgcgccatac aaactgggct gacaccgtac 
0701 aggaggcaaa aagctggctt tcatcggcgt tagcccaggc cgacacgctg gaagttggtc acggtattgg 
0771 tccggttcac cacttccacg cctggtggtg agcaccgtac tatagctgct ttggttattg tcagcctttg 
0841 gcactgacat tcagggatga aaccaatgca gctaagaatt accagtcgaa aaaaactcac c