|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
FEBS Lett. 1997;412(1):126-30.
Expression, purification and characterization of GDP-D-mannose 4,6-dehydratase from Escherichia coli.
Sturla L, Bisso A, Zanardi D, Benatti U, De Flora A, Tonetti M.
(
9257704
)
|
|
|
Deletion
|
16-1-2
(LD)
,
DE(sbcB-rfb)202
,
DE(his-dcd)453
,
OCL74-2,75-5 (LD 16-1-2_CRS)
(D)
,
OCL74-2,75-6 (LD 16-1-2_P/O)
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
gmd
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
gmd
Search strains by all related name:
gmd b2053 ECK2047 f373 JW2038 yefA yefN
|
General information (Go to Linear View: )
|
|
Gene Name
|
gmd
|
|
Alternative name
|
b2053,ECK2047,f373,JW2038,yefA,yefN
|
|
Location, Length
|
2,125,217 - 2,126,338
(
-
)
;
45.81
min
;
1122
(bp) ,
373
(aa)
|
|
Product
|
GDP-D-mannose dehydratase, NAD(P)-binding
|
|
Operon Name
|
wcaCDEF-gmd-fcl-gmm-wcaI-cpsBG-wcaJ-wzxC
|
|
Note
|
GO_component: GO:0005737 - cytoplasm; GO_function: GO:0042280 - cell surface antigen activity, host-interacting; GO_process: GO:0009242 - colanic acid biosynthetic process; GO_process: GO:0009435 - NAD biosynthetic process; GO_process: GO:0009269 - response to desiccation
|
|
Function
|
enzyme; Central intermediary metabolism: Sugar-nucleotide biosynthesis, conversions
|
|
Gene Ontology
|
GO:0003824
;
catalytic activity ( gmd )
GO:0005488
;
binding ( gmd )
GO:0005622
;
intracellular ( gmd )
GO:0008152
;
metabolic process ( gmd )
GO:0008446
;
GDP-mannose 4,6-dehydratase activity ( gmd )
GO:0009103
;
lipopolysaccharide biosynthetic process ( gmd )
GO:0016829
;
lyase activity ( gmd )
GO:0019673
;
GDP-mannose metabolic process ( gmd )
GO:0044237
;
cellular metabolic process ( gmd )
GO:0050662
;
coenzyme binding ( gmd )
|
|
PID
|
1788366
|
EC number
(KEGG Pathway)
|
4.2.1.47
|
|
SWISS-PROT
(
Show details
)
|
Entry name(Acc.no)
|
GM4D_ECOLI
(
P32054
;
P77687
)
|
|
- Protein name
|
GDP-mannose 4,6-dehydratase
|
|
- Synonyms
|
EC 4.2.1.47, GDP-D-mannose dehydratase
|
|
- Gene name
|
OrderedLocusNames=b2053, c2579, z3217, ECs2858, SF2116, S2239;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
gmd
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, E.Coli Gdp-Mannose 4,6-Dehydratase
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
GDP-mannose 4,6-dehydratase (GDP-D-mannose dehydratase)
|
|
PSI-BLAST
|
GDP-mannose 4,6-dehydratase (GDP-D-mannose dehydratase)
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
GDP-D-mannose dehydratase [Escherichia coli O157:H7 EDL933]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
ADH_SHORT
|
|
MMBR References
|
FEBS Lett. 1997;412(1):126-30.
Expression, purification and characterization of GDP-D-mannose 4,6-dehydratase from Escherichia coli.
Sturla L, Bisso A, Zanardi D, Benatti U, De Flora A, Tonetti M.
(
9257704
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSKVALITGV TGQDGSYLAE FLLEKGYEVH GIKRRASSFN TERVDHIYQD PHTCNPKFHL HYGDLSDTSN
0071 LTRILREVQP DEVYNLGAMS HVAVSFESPE YTADVDAMGT LRLLEAIRFL GLEKKTRFYQ ASTSELYGLV
0141 QEIPQKETTP FYPRSPYAVA KLYAYWITVN YRESYGMYAC NGILFNHESP RRGETFVTRK ITRAIANIAQ
0211 GLESCLYLGN MDSLRDWGHA KDYVKMQWMM LQQEQPEDFV IATGVQYSVR QFVEMAAAQL GIKLRFEGTG
0281 VEEKGIVVSV TGHDAPGVKP GDVIIAVDPR YFRPAEVETL LGDPTKAHEK LGWKPEITLR EMVSEMVAND
0351 LEAAKKHSLL KSHGYDVAIA LES
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 aaatcgcttc cggcaaatgt ggtttgccgg
-070 gggaatcccg cagtggtgat acgcgaacgc gttgaaactg aataaattca aaaatacaga ggaataatac
0001 atgtcaaaag tcgctctcat caccggtgta accggacaag acggttctta cctggcagag tttctgctgg
0071 aaaaaggtta cgaggtgcat ggtattaagc gtcgcgcatc gtcattcaac accgagcgcg tggatcacat
0141 ttatcaggat ccgcacacct gcaacccgaa attccatctg cattatggcg acctgagtga tacctctaac
0211 ctgacgcgca ttttgcgtga agtacagccg gatgaagtgt acaacctggg cgcaatgagc cacgttgcgg
0281 tctcttttga gtcaccagaa tataccgctg acgtcgacgc gatgggtacg ctgcgcctgc tggaggcgat
0351 ccgcttcctc ggtctggaaa agaaaactcg tttctatcag gcttccacct ctgaactgta tggtctggtg
0421 caggaaattc cgcagaaaga gaccacgccg ttctacccgc gatctccgta tgcggtcgcc aaactgtacg
0491 cctactggat caccgttaac taccgtgaat cctacggcat gtacgcctgt aacggaattc tcttcaacca
0561 tgaatccccg cgccgcggcg aaaccttcgt tacccgcaaa atcacccgcg caatcgccaa catcgcccag
0631 gggctggagt cgtgcctgta cctcggcaat atggattccc tgcgtgactg gggccacgcc aaagactacg
0701 taaaaatgca gtggatgatg ctgcagcagg aacagccgga agatttcgtt atcgcgaccg gcgttcagta
0771 ctccgtgcgt cagttcgtgg aaatggcggc agcacagctg ggcatcaaac tgcgctttga aggcacgggc
0841 gttgaagaga agggcattgt ggtttccgtc accgggcatg acgcgccggg cgttaaaccg ggtgatgtga
0911 ttatcgctgt tgacccgcgt tacttccgtc cggctgaagt tgaaacgctg ctcggcgacc cgaccaaagc
0981 gcacgaaaaa ctgggctgga aaccggaaat caccctcaga gagatggtgt ctgaaatggt ggctaatgac
1051 ctcgaagcgg cgaaaaaaca ctctctgctg aaatctcacg gctacgacgt ggcgatcgcg ctggagtcat
1121 aagcatgagt aaacaacgag tttttattgc tggtcatcgc gggatggtcg gttccgccat caggcggcag
1191 ctcgaacagc gcggtgatgt ggaactggta tt
|
