|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
|
|
Deletion
|
16-1-2
(LD)
,
DE(sbcB-rfb)202
,
DE(his-dcd)453
,
OCL74-2,75-5 (LD 16-1-2_CRS)
(D)
,
OCL74-2,75-6 (LD 16-1-2_P/O)
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
wcaH
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
wcaH
Search strains by all related name:
wcaH b2051 ECK2045 f160 gmm JW5335 nudD wcaH yefC
|
General information (Go to Linear View: )
|
|
Gene Name
|
wcaH
|
|
Alternative name
|
b2051,ECK2045,f160,gmm,JW5335,nudD,wcaH,yefC
|
|
Location, Length
|
2,123,767 - 2,124,246
(
-
)
;
45.77
min
;
480
(bp) ,
159
(aa)
|
|
Product
|
GDP-mannose mannosyl hydrolase
|
|
Operon Name
|
wcaCDEF-gmd-fcl-gmm-wcaI-cpsBG-wcaJ-wzxC
|
|
Note
|
GO_process: GO:0009226 - nucleotide-sugar biosynthetic process; GO_process: GO:0009242 - colanic acid biosynthetic process
|
|
Function
|
enzyme; Central intermediary metabolism: Sugar-nucleotide biosynthesis, conversions
|
|
Gene Ontology
|
GO:0000287
;
magnesium ion binding ( nudD )
GO:0009103
;
lipopolysaccharide biosynthetic process ( nudD )
GO:0016787
;
hydrolase activity ( nudD )
GO:0046872
;
metal ion binding ( nudD )
|
|
PID
|
87082031
|
EC number
(KEGG Pathway)
|
1.1.1.271
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
NUDD_ECOLI
(
P32056
)
|
|
- Protein name
|
GDP-mannose mannosyl hydrolase
|
|
- Synonyms
|
EC 3.6.1.-, GDPMH, Colanic acid biosynthesis protein wcaH
|
|
- Gene name
|
Name=nudD; Synonyms=wcaH, gmm; OrderedLocusNames=b2051;
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Homology Analysis
|
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BLAST
|
|
Bacteria
|
|
GTOP
|
nudD
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of The E. Coli Gdp-Mannose Mannosyl Hydrolase In Complex With Gdp And Mg
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
GDP-mannose mannosyl hydrolase
|
|
PSI-BLAST
|
Probable
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
Colanic acid biosynthesis protein wcaH - Escherichia coli (strain K-12)
|
|
Pfam 28.0
(database updated :
2015-05
)
|
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Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
AMIDATION
|
|
MMBR References
|
J Biol Chem. 1995;270:24086-24091
A novel GDP-mannose mannosyl hydrolase shares homology with the MutT family of enzymes.
Frick, D. N., B. D. Townsend, and M. J. Bessman.
|
| Sequences |
Amino acid
FASTA format
|
0001 MFLRQEDFAT VVRSTPLVSL DFIVENSRGE FLLGKRTNRP AQGYWFVPGG RVQKDETLEA AFERLTMAEL
0071 GLRLPITAGQ FYGVWQHFYD DNFSGTDFTT HYVVLGFRFR VSEEELLLPD EQHDDYRWLT SDALLASDNV
0141 HANSRAYFLA EKRTGVPGL
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 tgcatcagct tggctggtat cacgaaatct
-070 cactggaagc ggggcttgcc agcacttacc agtggttcct tgagaatcaa gaccgctttc gggggtaatg
0001 atgtttttac gtcaggaaga ctttgccacg gtagtgcgct ccactccgct tgtctctctc gactttattg
0071 tcgagaacag tcgcggcgag tttctgcttg gcaaaagaac caaccgcccg gcgcagggtt actggtttgt
0141 gccgggaggg cgcgtgcaga aagacgaaac gctggaagcc gcatttgagc ggctgacgat ggcggaactg
0211 gggctgcgtt tgccgataac agcaggccag ttttacggtg tctggcagca cttttatgac gataacttct
0281 ctggcacgga tttcaccact cactatgtgg tgctcggttt tcgcttcaga gtatcggaag aagagctgtt
0351 actgccggat gagcagcatg acgattaccg ctggctgacg tcggacgcgc tgctcgccag tgataatgtt
0421 catgctaaca gccgcgccta ttttctcgct gagaagcgta ccggagtacc cggattatga aaatactggt
0491 ctacggcatt aactactcgc cggagttaac cggcatcggc aaatacaccg gcgagatggt ggaatggctg
0561 gcggcacaag gtcatgaggt
|
