MG1655
W3110
Search for
Japanese | English
Top Genes Deletions Circular Linear Motif Contigs Reference SequenceCutter Download Strains TEC
Gene Report : cpsB
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion 16-1-2 (LD)  ,  DE(sbcB-rfb)202  ,  DE(his-dcd)453  ,  OCL74-2,75-5 (LD 16-1-2_CRS) (D)  ,  OCL74-2,75-6 (LD 16-1-2_P/O) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  cpsB
Related strains
     Strains Search Search strains by gene name:  cpsB   Search strains by all related name:  cpsB b2049 ECK2043 JW2034 manC mni rfbM

General information  (Go to Linear View:)
 Gene Name cpsB  
 Alternative name b2049,ECK2043,JW2034,manC,mni,rfbM  
 Location, Length 2,121,108 - 2,122,544 (  -  ) ;   45.72 min ; 1437 (bp) ,   478 (aa) Go to Linear View
 Product mannose-1-phosphate guanyltransferase  
 Operon Name wcaCDEF-gmd-fcl-gmm-wcaI-cpsBG-wcaJ-wzxC  
 Note GO_function: GO:0042280 - cell surface antigen activity, host-interacting; GO_process: GO:0009242 - colanic acid biosynthetic process; GO_process: GO:0016052 - carbohydrate catabolic process; GO_process: GO:0009226 - nucleotide-sugar biosynthetic process; GO_process: GO:0009269 - response to desiccation  
 Function enzyme; Central intermediary metabolism: Sugar-nucleotide biosynthesis, conversions  
 Gene Ontology GO:0005976 ; polysaccharide metabolic process ( manC )
GO:0008928 ; mannose-1-phosphate guanylyltransferase (GDP) activity ( manC )
GO:0009058 ; biosynthetic process ( manC )
GO:0009103 ; lipopolysaccharide biosynthetic process ( manC )
GO:0016740 ; transferase activity ( manC )
GO:0016779 ; nucleotidyltransferase activity ( manC )
 PID 1788362  
 EC number
  (KEGG Pathway)   		2.7.7.22  
SWISS-PROT  ( Show details [ 3 more] )
  botton Entry name(Acc.no) MANC_ECO57 ( O85342 )
    -  Protein name Mannose-1-phosphate guanylyltransferase [GDP]  
    -  Synonyms EC 2.7.7.22, GDP- mannose pyrophosphorylase, GMP  
    -  Gene name Name=manC; OrderedLocusNames=z3195, ECs2836;  

 Linear View (Whole Mode)
View Location
   2110.0  –  2135.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP cpsB (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Mannose-1-Phosphate Guanyltransferase From Thermus Thermophilus Hb8  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Mannose-1-phosphate guanylyltransferase [GDP]  
         PSI-BLAST Bifunctional protein glmU [Includes: UDP-N-acetylglucosamine pyrophosphorylase
    nr     (database updated : 2007.02.20 )
         BLAST mannose-1-P guanosyltransferase [Escherichia coli]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    MICROBODIES_CTER     

Other Cross-Reference
    COG COG0662G  COG0836M 
    EcoCyc cpsB 

MMBR References
J Bacteriol. 1984;160(1):184-91.
lon transcriptional regulation of genes necessary for capsular polysaccharide synthesis in Escherichia coli K-12.
Trisler P, Gottesman S. ( 6090411 )

Sequences
Amino acid
FASTA format 
0001 MAQSKLYPVV MAGGSGSRLW PLSRVLYPKQ FLCLKGDLTM LQTTICRLNG VECESPVVIC NEQHRFIVAE 
0071 QLRQLNKLTE NIILEPAGRN TAPAIALAAL AAKRHSPESD PLMLVLAADH VIADEDAFRA AVRNAMPYAE 
0141 AGKLVTFGIV PDLPETGYGY IRRGEVSAGE QDMVAFEVAQ FVEKPNLETA QAYVASGEYY WNSGMFLFRA 
0211 GRYLEELKKY RPDILDACEK AMSAVDPDLN FIRVDEEAFL ACPEESVDYA VMERTADAVV VPMDAGWSDV 
0281 GSWSSLWEIS AHTAEGNVCH GDVINHKTEN SYVYAESGLV TTVGVKDLVV VQTKDAVLIA DRNAVQDVKK 
0351 VVEQIKADGR HEHRVHREVY RPWGKYDSID AGDRYQVKRI TVKPGEGLSV QMHHHRAEHW VVVAGTAKVT 
0421 IDGDIKLLGE NESIYIPLGA THCLENPGKI PLDLIEVRSG SYLEEDDVVR FADRYGRV
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             tgctgcccaa acacaacacg gtggcacgtg 
-070 aatatgccga acgcacgctc gataaagaga acgtgttacg tcaatttata aatgatattc ggggataatt 
0001 atggcgcagt cgaaactcta tccagttgtg atggcaggtg gctccggtag ccgcttatgg ccgctttccc 
0071 gcgtacttta tcccaagcag tttttatgcc tgaaaggcga tctcaccatg ctgcaaacca ccatctgccg 
0141 cctgaacggc gtggagtgcg aaagcccggt ggtgatttgc aatgagcagc accgctttat tgtcgcggaa 
0211 cagctgcgtc aactgaacaa acttaccgag aacattattc tcgaaccggc agggcgaaac acggcacctg 
0281 ccattgcgct ggcggcgctg gcggcaaaac gtcatagccc ggagagcgac ccgttaatgc tggtattggc 
0351 ggcggatcat gtgattgccg atgaagacgc gttccgtgcc gccgtgcgta atgccatgcc atatgccgaa 
0421 gcgggcaagc tggtgacctt cggcattgtg ccggatctac cagaaaccgg ttatggctat attcgtcgcg 
0491 gtgaagtgtc tgcgggtgag caggatatgg tggcctttga agtggcgcag tttgtcgaaa aaccgaatct 
0561 ggaaaccgct caggcctatg tggcaagcgg cgaatattac tggaacagcg gtatgttcct gttccgcgcc 
0631 ggacgctatc tcgaagaact gaaaaaatat cgcccggata tcctcgatgc ctgtgaaaaa gcgatgagcg 
0701 ccgtcgatcc ggatctcaat tttattcgcg tggatgaaga agcgtttctc gcctgcccgg aagagtcggt 
0771 ggattacgcg gtcatggaac gtacggcaga tgctgttgtg gtgccgatgg atgcgggctg gagcgatgtt 
0841 ggctcctggt cttcattatg ggagatcagc gcccacaccg ccgagggcaa cgtttgccac ggcgatgtga 
0911 ttaatcacaa aactgaaaac agctatgtgt atgctgaatc tggcctggtc accaccgtcg gggtgaaaga 
0981 tctggtagtg gtgcagacca aagatgcggt gctgattgcc gaccgtaacg cggtacagga tgtgaaaaaa 
1051 gtggtcgagc agatcaaagc cgatggtcgc catgagcatc gggtgcatcg cgaagtgtat cgtccgtggg 
1121 gcaaatatga ctctatcgac gcgggcgacc gctaccaggt gaaacgcatc accgtgaaac cgggcgaggg 
1191 cttgtcggta cagatgcacc atcaccgcgc ggaacactgg gtggttgtcg cgggaacggc aaaagtcacc 
1261 attgatggtg atatcaaact gcttggtgaa aacgagtcca tttatattcc gctgggggcg acgcattgcc 
1331 tggaaaaccc ggggaaaatt ccgctcgatt taattgaagt gcgctccggc tcttatctcg aagaggatga 
1401 tgtggtgcgt ttcgcggatc gctacggacg ggtgtaaacg tcgcatcagg caatgaatgc gaaaccgcgg 
1471 tgtaaataac gacaaaaata aaattggccg cttcggtcag ggccaactat tgcctgaaaa agggtaa