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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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16-1-2
(LD)
,
DE(sbcB-rfb)202
,
DE(his-dcd)453
,
OCL74-2,75-5 (LD 16-1-2_CRS)
(D)
,
OCL74-2,75-6 (LD 16-1-2_P/O)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
rfbD
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Related strains
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Strains Search
|
Search strains by gene name:
rfbD
Search strains by all related name:
rfbD b2040 ECK2034 JW2025 rmlD som
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General information (Go to Linear View: )
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Gene Name
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rfbD
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Alternative name
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b2040,ECK2034,JW2025,rmlD,som
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Location, Length
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2,109,101 - 2,110,000
(
-
)
;
45.46
min
;
900
(bp) ,
299
(aa)
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Product
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dTDP-4-dehydrorhamnose reductase subunit, NAD(P)-binding, of dTDP-L-rhamnose synthase
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Operon Name
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rfbBDACX
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Note
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dTDP-6-deoxy-L-mannose-dehydrogenase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009243 - O antigen biosynthetic process; GO_process: GO:0009226 - nucleotide-sugar biosynthetic process
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Function
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enzyme; Central intermediary metabolism: Sugar-nucleotide biosynthesis, conversions
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Gene Ontology
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GO:0003824
;
catalytic activity ( rfbD )
GO:0005488
;
binding ( rfbD )
GO:0008152
;
metabolic process ( rfbD )
GO:0008831
;
dTDP-4-dehydrorhamnose reductase activity ( rfbD )
GO:0009103
;
lipopolysaccharide biosynthetic process ( rfbD )
GO:0016491
;
oxidoreductase activity ( rfbD )
GO:0045226
;
extracellular polysaccharide biosynthetic process ( rfbD )
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PID
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1788352
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EC number
(KEGG Pathway)
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1.1.1.133
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SWISS-PROT
(
Show details
)
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Entry name(Acc.no)
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RBD1_ECOLI
(
P37760
;
P76377
)
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- Protein name
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dTDP-4-dehydrorhamnose reductase
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- Synonyms
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EC 1.1.1.133, dTDP-4-keto-L- rhamnose reductase, dTDP-6-deoxy-L-mannose dehydrogenase, dTDP-L- rhamnose synthetase
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- Gene name
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Name=rfbD; Synonyms=rmlD; OrderedLocusNames=b2040;
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MMBR References
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Mol Microbiol. 1993;9(2):393-402.
Function of the rfb gene cluster and the rfe gene in the synthesis of O antigen by Shigella dysenteriae 1.
Klena JD, Schnaitman CA.
(
7692219
)
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J Bacteriol. 1994;176:4144-4156
Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster.
Stevenson, G., B. Neal, D. Liu, M. Hobbs, N. H. Packer, M. Batley, J. W. Redmond, L. Lindquist, and P. R. Reeves.
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J Bacteriol. 1994;176:4133-4143
Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a.
Yao, Z., and M. A. Valvano.
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| Sequences |
Amino acid
FASTA format
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0001 MNILLFGKTG QVGWELQRAL APLGNLIAFD VHSTDYCGDF SNPEGVAETV RSIRPDIIVN AAAHTAVDKA
0071 ESEPEFAQLI NATSVEAIAK AANEVGAWVI HYSTDYVFPG NGDMPWLETD ATAPLNVYGE TKLAGEKALQ
0141 EYCAKHLIFR TSWVYAGKGN NFAKTMLRLA KEREELAVIN DQFGAPTGAE LLADCTAHAI RVALNKPDVA
0211 GLYHLVASGT TTWYDYAALV FEEARKAGIP LALNKLNAVP TTAYPTPARR PHNSRLNTEK FQQNFALVLP
0281 DWQVGVKRML NELFTTTAI
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 aaacggtgga atggtacctg tccaatacaa
-070 aatgggttga taatgtgaaa agtggtgcct atcaatcgtg gattgaacag aactatgagg gccgccagta
0001 atgaatatcc tcctttttgg caaaacaggg caggtaggtt gggaactaca gcgtgctctg gcacctttgg
0071 gtaatttgat tgcttttgat gttcactcta ctgattattg cggtgatttt agtaatcctg aaggtgtagc
0141 tgaaaccgta agaagcattc ggccggatat tattgtcaat gcagccgctc acaccgcagt agacaaagca
0211 gaatcagaac cggagtttgc acaattaatt aacgcaacaa gtgtcgaagc gattgcgaaa gcagcaaatg
0281 aagttggagc ctgggttatc cattactcga ctgattacgt cttccctgga aatggcgata tgccatggct
0351 ggagacggat gcaaccgcac cactaaatgt ttacggtgaa accaagttag ccggagaaaa agcgttacag
0421 gaatattgcg cgaagcatct tattttccgg accagctggg tctatgcagg aaaaggaaat aacttcgcca
0491 aaacgatgtt acgtctggca aaagagcgtg aagaattagc ggttattaac gatcagtttg gtgcgccaac
0561 aggtgctgaa ctgctggctg attgtacagc acatgccatt cgtgtcgcac tgaataaacc ggatgtcgca
0631 ggcttgtacc atttggtagc cagtggtacc acaacctggt acgattatgc tgcgctggtt tttgaagagg
0701 cgcgcaaagc aggcattccc cttgcactca acaagctcaa cgcagtacca acaacagcct atcctacacc
0771 agctcgtcgt ccacataact ctcgccttaa tacagaaaaa tttcagcaga actttgcgct tgtcttgcct
0841 gactggcagg ttggcgtgaa acgaatgctc aatgaattat ttacgactac agcaatttaa tagtttttgc
0911 atcttgttcg tgatggtgga gcaagatgaa ttaaaaggaa tgatgaaatg aaaatgcgta aaggtattat
0981 tttagcgggt ggttctggta
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