MG1655
W3110
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Gene Report : rfbC
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion 16-1-2 (LD)  ,  DE(sbcB-rfb)202  ,  DE(his-dcd)453  ,  OCL74-2,75-5 (LD 16-1-2_CRS) (D)  ,  OCL74-2,75-6 (LD 16-1-2_P/O) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  rfbC
Related strains
     Strains Search Search strains by gene name:  rfbC   Search strains by all related name:  rfbC b2038 ECK2032 JW2023 rfbD rmlC

General information  (Go to Linear View:)
 Gene Name rfbC  
 Alternative name b2038,ECK2032,JW2023,rfbD,rmlC  
 Location, Length 2,107,605 - 2,108,162 (  -  ) ;   45.43 min ; 558 (bp) ,   185 (aa) Go to Linear View
 Product dTDP-4-deoxyrhamnose-3,5-epimerase  
 Operon Name rfbBDACX  
 Note dTDP-6-deoxy-D-glucose-3,5 epimerase; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0042803 - protein homodimerization activity; GO_process: GO:0009226 - nucleotide-sugar biosynthetic process; GO_process: GO:0009243 - O antigen biosynthetic process; GO_process: GO:0009103 - lipopolysaccharide biosynthetic process; GO_process: GO:0019305 - dTDP-rhamnose biosynthetic process  
 Function enzyme; Central intermediary metabolism: Sugar-nucleotide biosynthesis, conversions  
 Gene Ontology GO:0003824 ; catalytic activity ( rfbD )
GO:0005488 ; binding ( rfbD )
GO:0005515 ; protein binding ( rfbC )
GO:0008152 ; metabolic process ( rfbD )
GO:0008830 ; dTDP-4-dehydrorhamnose 3,5-epimerase activity ( rfbC )
GO:0008831 ; dTDP-4-dehydrorhamnose reductase activity ( rfbD )
GO:0009103 ; lipopolysaccharide biosynthetic process ( rfbC )
GO:0009103 ; lipopolysaccharide biosynthetic process ( rfbD )
GO:0016491 ; oxidoreductase activity ( rfbD )
GO:0016853 ; isomerase activity ( rfbC )
GO:0045226 ; extracellular polysaccharide biosynthetic process ( rfbD )
 PID 1788350  
 EC number
  (KEGG Pathway)
 
5.1.3.13  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) RFBC_ECOLI ( P37745 )
    -  Protein name dTDP-4-dehydrorhamnose 3,5-epimerase  
    -  Synonyms EC 5.1.3.13, dTDP-4-keto-6- deoxyglucose 3,5-epimerase, dTDP-L-rhamnose synthetase  
    -  Gene name Name=rfbC; Synonyms=rmlC; OrderedLocusNames=b2038;  

 Linear View (Whole Mode)
View Location
   2095.0  –  2120.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP rfbC (  homologous genes of other bacterias  )   rfbD (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Rmlc From Salmonella Typhimurium  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST dTDP-4-dehydrorhamnose 3,5-epimerase  
         PSI-BLAST dTDP-4-dehydrorhamnose 3,5-epimerase
    nr     (database updated : 2007.02.20 )
         BLAST dTDP-4-deoxyrhamnose-3,5-epimerase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG1898M 
    EcoCyc rfbC 

MMBR References
J Bacteriol. 1994;176:4133-4143
Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a.
Yao, Z., and M. A. Valvano.

Sequences
Amino acid
FASTA format
0001 MNVIRTEIED VLILEPRVFG DDRGFFYESF NQSAFEHILG YPVSFVQDNH SRSSKNVLRG LHFQRGEYAQ 
0071 DKLVRCTHGA VFDVAVDIRP NSVSFGKWVG VLLSADNKQQ LWIPKGFAHG FLVLSDIAEF QYKTTNYYHP 
0141 ESDCGICWND ERIAIDWPQT SGLILSPKDE RLFTLDELIR LKLIA

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             aaggttttat tgatgttgag caagtaagaa 
-070 aattagctgt accactaata aagaataatt atgggcagta tctttataaa atgacgaagg attcaaatta 
0001 atgaatgtga ttagaactga aattgaagat gtgctaattc tggagccaag agtatttggt gatgatagag 
0071 gtttctttta tgagagcttt aatcaatcag catttgaaca tattctaggc tatccggtca gctttgttca 
0141 agacaatcac tcacgttcat caaaaaatgt actcagaggc cttcactttc aacgcggcga gtacgcacaa 
0211 gataaacttg tacgctgcac tcatggagca gtttttgatg ttgctgttga tattcgaccc aattcggtat 
0281 cctttggtaa atgggttggt gttctgcttt cagctgataa taagcagcag ttgtggatac caaaagggtt 
0351 tgctcatggc tttttggttc tgtctgatat cgctgaattt caatataaaa ctacaaacta ttatcatcct 
0421 gaaagcgatt gtggaatatg ttggaatgat gaacgcattg caattgattg gccccaaaca tcagggttaa 
0491 tcctttcgcc aaaagatgaa aggctcttta cgttagatga gcttatcaga ttaaaattaa ttgcatgaat 
0561 acgaataaat tatctttaag aagaaacgtt atatatctgg ctgtcgttca aggtagcaat tatcttttac 
0631 cattgcttac atttccatat cttgtaag