MG1655
W3110
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Gene Report : thrA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion thrA34::Tn10  ,  OCR27 (D)  ,  OCR27-1 (D)  ,  OCR27-6 (D)  ,  OCR27-7 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  thrA
Related strains
     Strains Search Search strains by gene name:  thrA   Search strains by all related name:  thrA b0002 ECK0002 Hs JW0001 thrA1 thrA2 thrD

General information  (Go to Linear View:)
 Gene Name thrA  
 Alternative name b0002,ECK0002,Hs,JW0001,thrA1,thrA2,thrD  
 Location, Length 337 - 2,799 (  +  ) ;   0.01 min ; 2463 (bp) ,   820 (aa) Go to Linear View
 Product fused aspartokinase I and homoserine dehydrogenase I  
 Operon Name thrLABC  
 Note bifunctional: aspartokinase I (N-terminal); homoserine dehydrogenase I (C-terminal); GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009088 - threonine biosynthetic process; GO_process: GO:0009086 - methionine biosynthetic process; GO_process: GO:0009090 - homoserine biosynthetic process  
 Function enzyme; Amino acid biosynthesis: Threonine,1.5.1.8 metabolism; building block biosynthesis; amino acids; threonine,1.5.1.21 metabolism; building block biosynthesis; amino acids; homoserine,7.1 location of gene products; cytoplasm  
 Gene Ontology GO:0003824; catalytic activity ( thrA )
GO:0004072; aspartate kinase activity ( thrA )
GO:0004412; homoserine dehydrogenase activity ( thrA )
GO:0005488; binding ( thrA )
GO:0008152; metabolic process ( thrA )
GO:0008652; amino acid biosynthetic process ( thrA )
GO:0009067; aspartate family amino acid biosynthetic process ( thrA )
GO:0009088; threonine biosynthetic process ( thrA )
GO:0016301; kinase activity ( thrA )
GO:0016491; oxidoreductase activity ( thrA )
GO:0016597; amino acid binding ( thrA )
GO:0016740; transferase activity ( thrA )
 PID 1786183  
 EC number
  (KEGG Pathway)
 
1.1.1.3   2.7.2.4  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene thrA ( EG10998 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) AK1H_ECOLI ( P00561 ; Q47659 )
    -  Protein name Bifunctional aspartokinase/homoserine dehydrogenase I  
    -  Synonyms AKI-HDI  
    -  Gene name Name=thrA; Synonyms=thrA1, thrA2; OrderedLocusNames=b0002;  

 Linear View (Whole Mode)
View Location
   4625.0  –  25.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP thrA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Arabidopsis Thaliana Aspartate Kinase Complexed With Lysine And S-Adenosylmethionine  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Bifunctional aspartokinase/homoserine dehydrogenase I  
         PSI-BLAST Bifunctional aspartokinase/homoserine dehydrogenase I
    nr     (database updated : 2007.02.20 )
         BLAST bifunctional aspartokinase I/homeserine dehydrogenase I [Escherichia coli APEC O1]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    ASPARTOKINASE    HOMOSER_DHGENASE     

Other Cross-Reference
    COG COG0460E  COG0527E 
    EcoCyc thrA 

MMBR References
J Bacteriol. 1979;140(1):106-13.
Structural and functional analysis of cloned deoxyribonucleic acid containing the trpR-thr region of the Escherichia coli chromosome.
Gunsalus RP, Zurawski G, Yanofsky C. ( 159284 )
Proc Natl Acad Sci U S A. 1980;77:5730-5733
Nucleotide sequence of the thrA gene of Escherichia coli.
Katinka, M., P. Cossart, L. Sibilli, I. Saint-Girons, M. A. Chalvignac, G. LeBras, G. N. Cohen, and M. Yaniv.
J Mol Biol. 1985;183(4):529-41.
Identification and characterization of mutants affecting transcription termination at the threonine operon attenuator.
Lynn SP, Bauer CE, Chapman K, Gardner JF. ( 2410621 )
J Biol Chem. 1995;270:23330-23336
Transcription termination at the thr attenuator. Evidence that the adenine residues upstream of the stem and loop structure are not required for termination.
Yang, M. T., H. B. N. Scott, and J. F. Gardner.
Nucleic Acids Res. 1981;9:339-347
Nucleotide sequence of the thrB gene of E. coli, and its two adjacent regions; the thrAB and thrBC junctions.
Cossart, P., M. Katinka, and M. Yaniv.
Mol Gen Genet. 1982;188(2):225-7.
Attenuation in the threonine operon: effects of amino acids present in the presumed leader peptide in addition to threonine and isoleucine.
Daniel J, Saint-Girons I. ( 6818426 )

Sequences
Amino acid
FASTA format
0001 MRVLKFGGTS VANAERFLRV ADILESNARQ GQVATVLSAP AKITNHLVAM IEKTISGQDA LPNISDAERI 
0071 FAELLTGLAA AQPGFPLAQL KTFVDQEFAQ IKHVLHGISL LGQCPDSINA ALICRGEKMS IAIMAGVLEA 
0141 RGHNVTVIDP VEKLLAVGHY LESTVDIAES TRRIAASRIP ADHMVLMAGF TAGNEKGELV VLGRNGSDYS 
0211 AAVLAACLRA DCCEIWTDVD GVYTCDPRQV PDARLLKSMS YQEAMELSYF GAKVLHPRTI TPIAQFQIPC 
0281 LIKNTGNPQA PGTLIGASRD EDELPVKGIS NLNNMAMFSV SGPGMKGMVG MAARVFAAMS RARISVVLIT 
0351 QSSSEYSISF CVPQSDCVRA ERAMQEEFYL ELKEGLLEPL AVTERLAIIS VVGDGMRTLR GISAKFFAAL 
0421 ARANINIVAI AQGSSERSIS VVVNNDDATT GVRVTHQMLF NTDQVIEVFV IGVGGVGGAL LEQLKRQQSW 
0491 LKNKHIDLRV CGVANSKALL TNVHGLNLEN WQEELAQAKE PFNLGRLIRL VKEYHLLNPV IVDCTSSQAV 
0561 ADQYADFLRE GFHVVTPNKK ANTSSMDYYH QLRYAAEKSR RKFLYDTNVG AGLPVIENLQ NLLNAGDELM 
0631 KFSGILSGSL SYIFGKLDEG MSFSEATTLA REMGYTEPDP RDDLSGMDVA RKLLILARET GRELELADIE 
0701 IEPVLPAEFN AEGDVAAFMA NLSQLDDLFA ARVAKARDEG KVLRYVGNID EDGVCRVKIA EVDGNDPLFK 
0771 VKNGENALAF YSHYYQPLPL VLRGYGAGND VTAAGVFADL LRTLSWKLGV 

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             AGGTAACGGT GCGGGCTGAC GCGTACAGGA 
-070 AACACAGAAA AAAGCCCGCA CCTGACAGTG CGGGCTTTTT TTTTCGACCA AAGGTAACGA GGTAACAACC 
0001 atgcgagtgt tgaagttcgg cggtacatca gtggcaaatg cagaacgttt tctgcgtgtt gccgatattc 
0071 tggaaagcaa tgccaggcag gggcaggtgg ccaccgtcct ctctgccccc gccaaaatca ccaaccacct 
0141 ggtggcgatg attgaaaaaa ccattagcgg ccaggatgct ttacccaata tcagcgatgc cgaacgtatt 
0211 tttgccgaac ttttgacggg actcgccgcc gcccagccgg ggttcccgct ggcgcaattg aaaactttcg 
0281 tcgatcagga atttgcccaa ataaaacatg tcctgcatgg cattagtttg ttggggcagt gcccggatag 
0351 catcaacgct gcgctgattt gccgtggcga gaaaatgtcg atcgccatta tggccggcgt attagaagcg 
0421 cgcggtcaca acgttactgt tatcgatccg gtcgaaaaac tgctggcagt ggggcattac ctcgaatcta 
0491 ccgtcgatat tgctgagtcc acccgccgta ttgcggcaag ccgcattccg gctgatcaca tggtgctgat 
0561 ggcaggtttc accgccggta atgaaaaagg cgaactggtg gtgcttggac gcaacggttc cgactactct 
0631 gctgcggtgc tggctgcctg tttacgcgcc gattgttgcg agatttggac ggacgttgac ggggtctata 
0701 cctgcgaccc gcgtcaggtg cccgatgcga ggttgttgaa gtcgatgtcc taccaggaag cgatggagct 
0771 ttcctacttc ggcgctaaag ttcttcaccc ccgcaccatt acccccatcg cccagttcca gatcccttgc 
0841 ctgattaaaa ataccggaaa tcctcaagca ccaggtacgc tcattggtgc cagccgtgat gaagacgaat 
0911 taccggtcaa gggcatttcc aatctgaata acatggcaat gttcagcgtt tctggtccgg ggatgaaagg 
0981 gatggtcggc atggcggcgc gcgtctttgc agcgatgtca cgcgcccgta tttccgtggt gctgattacg 
1051 caatcatctt ccgaatacag catcagtttc tgcgttccac aaagcgactg tgtgcgagct gaacgggcaa 
1121 tgcaggaaga gttctacctg gaactgaaag aaggcttact ggagccgctg gcagtgacgg aacggctggc 
1191 cattatctcg gtggtaggtg atggtatgcg caccttgcgt gggatctcgg cgaaattctt tgccgcactg 
1261 gcccgcgcca atatcaacat tgtcgccatt gctcagggat cttctgaacg ctcaatctct gtcgtggtaa 
1331 ataacgatga tgcgaccact ggcgtgcgcg ttactcatca gatgctgttc aataccgatc aggttatcga 
1401 agtgtttgtg attggcgtcg gtggcgttgg cggtgcgctg ctggagcaac tgaagcgtca gcaaagctgg 
1471 ctgaagaata aacatatcga cttacgtgtc tgcggtgttg ccaactcgaa ggctctgctc accaatgtac 
1541 atggccttaa tctggaaaac tggcaggaag aactggcgca agccaaagag ccgtttaatc tcgggcgctt 
1611 aattcgcctc gtgaaagaat atcatctgct gaacccggtc attgttgact gcacttccag ccaggcagtg 
1681 gcggatcaat atgccgactt cctgcgcgaa ggtttccacg ttgtcacgcc gaacaaaaag gccaacacct 
1751 cgtcgatgga ttactaccat cagttgcgtt atgcggcgga aaaatcgcgg cgtaaattcc tctatgacac 
1821 caacgttggg gctggattac cggttattga gaacctgcaa aatctgctca atgcaggtga tgaattgatg 
1891 aagttctccg gcattctttc tggttcgctt tcttatatct tcggcaagtt agacgaaggc atgagtttct 
1961 ccgaggcgac cacgctggcg cgggaaatgg gttataccga accggacccg cgagatgatc tttctggtat 
2031 ggatgtggcg cgtaaactat tgattctcgc tcgtgaaacg ggacgtgaac tggagctggc ggatattgaa 
2101 attgaacctg tgctgcccgc agagtttaac gccgagggtg atgttgccgc ttttatggcg aatctgtcac 
2171 aactcgacga tctctttgcc gcgcgcgtgg cgaaggcccg tgatgaagga aaagttttgc gctatgttgg 
2241 caatattgat gaagatggcg tctgccgcgt gaagattgcc gaagtggatg gtaatgatcc gctgttcaaa 
2311 gtgaaaaatg gcgaaaacgc cctggccttc tatagccact attatcagcc gctgccgttg gtactgcgcg 
2381 gatatggtgc gggcaatgac gttacagctg ccggtgtctt tgctgatctg ctacgtaccc tctcatggaa 
2451 gttaggagtc tgaCATGGTT AAAGTTTATG CCCCGGCTTC CAGTGCCAAT ATGAGCGTCG GGTTTGATGT 
2521 GCTCGGGGCG GCGGTGACAC CTGTTGATGG TGCATTGCTC GGA