|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
|
|
Deletion
|
LD3-5-2
(LD)
,
OCL75
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
sbcB
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
sbcB
Search strains by all related name:
sbcB b2011 cpeA ECK2005 JW1993 xonA
|
General information (Go to Linear View: )
|
|
Gene Name
|
sbcB
|
|
Alternative name
|
b2011,cpeA,ECK2005,JW1993,xonA
|
|
Location, Length
|
2,080,780 - 2,082,207
(
+
)
;
44.85
min
;
1428
(bp) ,
475
(aa)
|
|
Product
|
exonuclease I
|
|
Operon Name
|
sbcB
|
|
Note
|
exonuclease I, 3' --> 5' specific; deoxyribophosphodiesterase; GO_process: GO:0006308 - DNA catabolic process
|
|
Function
|
enzyme; Degradation of DNA
|
|
Gene Ontology
|
GO:0003676
;
nucleic acid binding ( sbcB )
GO:0004518
;
nuclease activity ( sbcB )
GO:0004527
;
exonuclease activity ( sbcB )
GO:0006281
;
DNA repair ( sbcB )
GO:0006974
;
response to DNA damage stimulus ( sbcB )
GO:0008852
;
exodeoxyribonuclease I activity ( sbcB )
GO:0016787
;
hydrolase activity ( sbcB )
|
|
PID
|
1788321
|
EC number
(KEGG Pathway)
|
3.1.11.1
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
sbcB
(
EG10926
)
|
|
Number of removed
|
0 aa cleaved
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
EX1_ECOLI
(
P04995
)
|
|
- Protein name
|
Exodeoxyribonuclease I
|
|
- Synonyms
|
EC 3.1.11.1, Exonuclease I, DNA deoxyribophosphodiesterase, dRPase
|
|
- Gene name
|
Name=sbcB; Synonyms=xonA, cpeA; OrderedLocusNames=b2011;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
sbcB
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, The Structure Of Exonuclease I Suggests How Processivity Is Achieved
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Exodeoxyribonuclease I
|
|
PSI-BLAST
|
Exodeoxyribonuclease I
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
exonuclease I [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
|
|
MMBR References
|
J Bacteriol. 1976;126(2):999-1001.
Altered deoxyribonucleotide pools in P2 eductants of Escherichia coli K-12 due to deletion of the dcd gene.
Neuhard J, Thomassen E.
(
177407
)
|
J Bacteriol. 1988;170(5):2089-94.
Physical and biochemical characterization of cloned sbcB and xonA mutations from Escherichia coli K-12.
Phillips GJ, Prasher DC, Kushner SR.
(
2834321
)
|
J Biol Chem. 1987;262:455-459
Determination of the nucleotide sequence for the exonuclease I structural gene (sbcB) of Escherichia coli K-12.
Phillips, G. J., and S. R. Kushner.
|
J Bacteriol. 1983;153(2):903-8.
Physical and genetic characterization of the cloned sbcB (exonuclease I) region of the Escherichia coli genome.
Prasher D, Kasunic DA, Kushner SR.
(
6296059
)
|
Nucleic Acids Res. 1992;20:4699-4703
DNA deoxyribophosphodiesterase of Escherichia coli is associated with exonuclease I.
Sandigursky, M., and W. A. Franklin.
|
J Bacteriol. 1971;108(2):695-704.
Extent of host deletions associated with bacteriophage P2-mediated eduction.
Sunshine MG, Kelly B.
(
4942760
)
|
Genetics 1996;143:27-36
Orientation dependence in homologous recombination.
Yamamoto, K., N. Takahashi, Y. Fujitani, H. Yoshikura, and I. Kobayashi.
|
| Sequences |
Amino acid
FASTA format
|
0001 MMNDGKQQST FLFHDYETFG THPALDRPAQ FAAIRTDSEF NVIGEPEVFY CKPADDYLPQ PGAVLITGIT
0071 PQEARAKGEN EAAFAARIHS LFTVPKTCIL GYNNVRFDDE VTRNIFYRNF YDPYAWSWQH DNSRWDLLDV
0141 MRACYALRPE GINWPENDDG LPSFRLEHLT KANGIEHSNA HDAMADVYAT IAMAKLVKTR QPRLFDYLFT
0211 HRNKHKLMAL IDVPQMKPLV HVSGMFGAWR GNTSWVAPLA WHPENRNAVI MVDLAGDISP LLELDSDTLR
0281 ERLYTAKTDL GDNAAVPVKL VHINKCPVLA QANTLRPEDA DRLGINRQHC LDNLKILREN PQVREKVVAI
0351 FAEAEPFTPS DNVDAQLYNG FFSDADRAAM KIVLETEPRN LPALDITFVD KRIEKLLFNY RARNFPGTLD
0421 YAEQQRWLEH RRQVFTPEFL QGYADELQML VQQYADDKEK VALLKALWQY AEEIV
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ATGATCTGTT GCCACTCGCT GCCAAATTGT
-070 GGCGCTAAAG CTGATTAGCA CGGTGATATT TGATACTCTG GCAGACAGCA GAAATAACGG ATTTAACCTA
0001 atgatgaatg acggtaagca acaatctacc tttttgtttc acgattacga aacctttggc acgcaccccg
0071 cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga tagcgaattc aatgtcatcg gcgaacccga
0141 agtcttttac tgcaagcccg ctgatgacta tttaccccag ccaggagccg tattaattac cggtattacc
0211 ccgcaggaag cacgggcgaa aggagaaaac gaagccgcgt ttgccgcccg tattcactcg ctttttaccg
0281 taccgaagac ctgtattctg ggctacaaca atgtgcgttt cgacgacgaa gtcacacgca acatttttta
0351 tcgtaatttc tacgatcctt acgcctggag ctggcagcat gataactcgc gctgggattt actggatgtt
0421 atgcgtgcct gttatgccct gcgcccggaa ggaataaact ggcctgaaaa tgatgacggt ctaccgagct
0491 ttcgccttga gcatttaacc aaagcgaatg gtattgaaca tagcaacgcc cacgatgcga tggctgatgt
0561 gtacgccact attgcgatgg caaagctggt aaaaacgcgt cagccacgcc tgtttgatta tctctttacc
0631 catcgtaata aacacaaact gatggcgttg attgatgttc cgcagatgaa acccctggtg cacgtttccg
0701 gaatgtttgg agcatggcgc ggcaatacca gctgggtggc accgctggcg tggcatcctg aaaatcgcaa
0771 tgccgtaatt atggtggatt tggcaggaga catttcgcca ttactggaac tggatagcga cacattgcgc
0841 gagcgtttat ataccgcaaa aaccgatctt ggcgataacg ccgccgttcc ggttaagctg gtgcatatca
0911 ataaatgtcc ggtgctggcc caggcgaata cgctacgccc ggaagatgcc gaccgactgg gaattaatcg
0981 tcagcattgc ctcgataacc tgaaaattct gcgtgaaaat ccgcaagtgc gcgaaaaagt ggtggcgata
1051 ttcgcggaag ccgaaccgtt tacgccttca gataacgtgg atgcacagct ttataacggc tttttcagtg
1121 acgcagatcg tgcagcaatg aaaattgtgc tggaaaccga gccgcgtaat ttaccggcac tggatatcac
1191 ttttgttgat aaacggattg aaaagctgtt gttcaattat cgggcacgca acttcccggg gacgctggat
1261 tatgccgagc agcaacgctg gctggagcac cgtcgccagg tcttcacgcc agagtttttg cagggttatg
1331 ctgatgaatt gcagatgctg gtacaacaat atgccgatga caaagagaaa gtggcgctgt taaaagcact
1401 ttggcagtac gcggaagaga ttgtctaaTT TGAAGCCTCC GCCGCTGGTA CGGCGGAGGA CAATGTTGAG
1471 TCAGGCTTTT TGAACGGTGA TGCTCCACGC TGCATCGCCA ATCTGCTGAT AATCGGTG
|
