MG1655
W3110
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Gene Report : araF
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Protein Sci. 1992;1(12):1652-60.
Escherichia coli K12 arabinose-binding protein mutants with altered transport properties.
Kehres DG, Hogg RW. ( 1304895 )
     Deletion LD3-32-1 (LD)  ,  OCL79 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  araF
Related strains
     Strains Search Search strains by gene name:  araF   Search strains by all related name:  araF b1901 ECK1899 JW1889

General information  (Go to Linear View:)
 Gene Name araF  
 Alternative name b1901,ECK1899,JW1889  
 Location, Length 1,983,163 - 1,984,152 (  -  ) ;   42.74 min ; 990 (bp) ,   329 (aa) Go to Linear View
 Product L-arabinose transporter subunit  
 Operon Name araFGH  
 Note L-arabinose-binding periplasmic protein; periplasmic-binding component of ABC superfamily; GO_component: GO:0042597 - periplasmic space; GO_process: GO:0016052 - carbohydrate catabolic process  
 Function transport; Transport of small molecules: Carbohydrates, organic acids, alcohols  
 Gene Ontology GO:0005351; sugar:hydrogen ion symporter activity ( araF )
GO:0006810; transport ( araF )
GO:0008643; carbohydrate transport ( araF )
GO:0042597; periplasmic space ( araF )
 PID 1788211  
 EC number
  (KEGG Pathway)
 
 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene araF ( EG10057 )  
    Number of removed 23 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) ARAF_ECOLI ( P02924 )
    -  Protein name L-arabinose-binding periplasmic protein precursor  
    -  Synonyms ABP  
    -  Gene name Name=araF; OrderedLocusNames=b1901;  

 Linear View (Whole Mode)
View Location
   1970.0  –  1995.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP araF (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain , L-Arabinose-Binding Protein Complex With D-Galactose  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST L-arabinose-binding periplasmic protein precursor  
         PSI-BLAST 6-phosphofructokinase
    nr     (database updated : 2007.02.20 )
         BLAST L-arabinose transporter subunit [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG1879G 
    EcoCyc araF 

MMBR References
J Bacteriol. 1981;148(2):472-9.
L-arabinose transport systems in Escherichia coli K-12.
Kolodrubetz D, Schleif R. ( 7028715 )
J Mol Biol. 1981;151(2):215-27.
Regulation of the L-arabinose transport operons in Escherichia coli.
Kolodrubetz D, Schleif R. ( 6461771 )
J Mol Biol. 1982;156(1):53-66.
Arabinose-inducible promoter from Escherichia coli. Its cloning from chromosomal DNA, identification as the araFG promoter and sequence.
Kosiba BE, Schleif R. ( 7047752 )
J Mol Biol. 1987;197(1):37-46.
High-affinity L-arabinose transport operon. Nucleotide sequence and analysis of gene products.
Scripture JB, Voelker C, Miller S, O'Donnell RT, Polgar L, Rade J, Horazdovsky BF, Hogg RW. ( 2445996 )
J Biol Chem. 1983;258:10853-10855
The nucleotide sequences defining the signal peptides of the galactose-binding protein and the arabinose-binding protein.
Scripture, J. B., and R. W. Hogg.
J Bacteriol. 1981;147(3):920-4.
High-affinity arabinose transport mutants of Escherichia coli: isolation and gene location.
Clark AF, Hogg RW. ( 7024251 )

Sequences
Amino acid
FASTA format
0001 MHKFTKALAA IGLAAVMSQS AMAENLKLGF LVKQPEEPWF QTEWKFADKA GKDLGFEVIK IAVPDGEKTL 
0071 NAIDSLAASG AKGFVICTPD PKLGSAIVAK ARGYDMKVIA VDDQFVNAKG KPMDTVPLVM MAATKIGERQ 
0141 GQELYKEMQK RGWDVKESAV MAITANELDT ARRRTTGSMD ALKAAGFPEK QIYQVPTKSN DIPGAFDAAN 
0211 SMLVQHPEVK HWLIVGMNDS TVLGGVRATE GQGFKAADII GIGINGVDAV SELSKAQATG FYGSLLPSPD 
0281 VHGYKSSEML YNWVAKDVEP PKFTEVTDVV LITRDNFKEE LEKKGLGGK

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             ttatctttta gttaaaaggt aatgctttgt 
-070 tttccgatta atttaacgaa tgtcattcgt ttttgcccta cacaaaacga cactaaagct ggagagaacc 
0001 atgcacaaat ttactaaagc cctggcagcc attggtctgg cagccgttat gtcacaatcc gctatggcgg 
0071 agaacctgaa gctcggtttt ctggtgaagc aaccggaaga gccgtggttc cagaccgaat ggaagtttgc 
0141 cgataaagcc gggaaggatt tagggtttga ggttattaag attgccgtgc cggatggcga aaaaacattg 
0211 aacgcgatcg acagcctggc tgccagtggc gcaaaaggtt tcgttatttg tactccggac cccaaactcg 
0281 gctctgccat cgtcgcgaaa gcgcgtggct acgatatgaa agtcattgcc gtggatgacc agtttgttaa 
0351 cgccaaaggt aagccaatgg ataccgttcc gctggtgatg atggcggcga ctaaaattgg cgaacgtcag 
0421 ggccaggaac tgtataaaga gatgcagaaa cgtggctggg atgtcaaaga aagcgcggtg atggcgatta 
0491 ccgccaacga actggatacc gcccgccgcc gtactacggg atctatggat gcgctgaaag cggccggatt 
0561 cccggaaaaa caaatttatc aggtacctac caaatctaac gacatcccgg gggcatttga cgctgccaac 
0631 tcaatgctgg ttcaacatcc ggaagttaaa cattggctga tcgtcggtat gaacgacagc accgtgctgg 
0701 gcggcgtacg cgcgacggaa ggtcagggct ttaaagcggc cgatatcatc ggcattggca ttaacggtgt 
0771 ggatgcggtg agcgaactgt ctaaagcaca ggcaaccggc ttctacggtt ccctgctgcc aagcccggac 
0841 gtacatggct ataaatccag cgaaatgctt tacaactggg tagcaaaaga cgttgaaccg ccaaaattta 
0911 ccgaagttac cgacgtggta ctgatcacgc gtgacaactt taaagaagaa ctggagaaaa aaggtttagg 
0981 cggtaagtaa tttgccggaa aaattcccct ctgcatgatg cagagggggt gtgaacgacc agtgattcac 
1051 ggagacgtta tgcaacagtc taccccgtat ctctcatttc