MG1655
W3110
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Gene Report : argS
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
     Deletion OCL79,81-3 (BK5-5 / AK4-5) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  argS
Related strains
     Strains Search Search strains by gene name:  argS   Search strains by all related name:  argS b1876 ECK1877 JW1865 lov

General information  (Go to Linear View:)
 Gene Name argS  
 Alternative name b1876,ECK1877,JW1865,lov  
 Location, Length 1,958,086 - 1,959,819 (  +  ) ;   42.20 min ; 1734 (bp) ,   577 (aa) Go to Linear View
 Product arginyl-tRNA synthetase  
 Operon Name argS  
 Note arginine tRNA synthetase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006418 - tRNA aminoacylation for protein translation  
 Function enzyme; Aminoacyl tRNA synthetases, tRNA modification  
 Gene Ontology GO:0000166; nucleotide binding ( argS )
GO:0004812; aminoacyl-tRNA ligase activity ( argS )
GO:0004814; arginine-tRNA ligase activity ( argS )
GO:0005524; ATP binding ( argS )
GO:0005737; cytoplasm ( argS )
GO:0006412; translation ( argS )
GO:0006418; tRNA aminoacylation for protein translation ( argS )
GO:0006420; arginyl-tRNA aminoacylation ( argS )
GO:0016874; ligase activity ( argS )
 PID 1788184  
 EC number
  (KEGG Pathway)
 
6.1.1.19  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene argS ( EG10071 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) SYR_ECOLI ( P11875 )
    -  Protein name Arginyl-tRNA synthetase  
    -  Synonyms EC 6.1.1.19, Arginine--tRNA ligase, ArgRS  
    -  Gene name Name=argS; OrderedLocusNames=b1876;  

 Linear View (Whole Mode)
View Location
   1945.0  –  1970.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP argS (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Yeast Arginyl-Trna Synthetase  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Arginyl-tRNA synthetase  
         PSI-BLAST Arginyl-tRNA synthetase
    nr     (database updated : 2007.02.20 )
         BLAST arginyl-tRNA synthetase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AA_TRNA_LIGASE_I     

Other Cross-Reference
    COG COG0018J 
    EcoCyc argS 

MMBR References
Nucleic Acids Res. 1989;17:5725-5736
Isolation and characterization of the gene coding for Escherichia coli arginyl-tRNA synthetase.
Eriani, G., G. Dirheimer, and J. Gangloff.
J Mol Biol. 1968;35(1):83-93.
Studies on the mechanism of repression of arginine biosynthesis in Escherichia coli. 3. Repression of enzymes of arginine biosynthesis in arginyl-tRNA synthetase mutants.
Hirshfield IN, Horn PC, Hopwood DA, Maas WK, DeDeken R. ( 4939781 )
Microbiol. Rev. 1983;47:231-284.
Gene-protein index of Escherichia coli K-12.
Neidhardt, F. C., V. Vaughn, T. A. Phillips, and P. L. Bloch.
EMBO J. 1992;11:1493-1501
Penicillin binding protein 2 is dispensable in Escherichia coli when ppGpp synthesis is induced.
Vinella, D., R. D'Ari, and P. Bouloc.

Sequences
Amino acid
FASTA format
0001 MNIQALLSEK VRQAMIAAGA PADCEPQVRQ SAKVQFGDYQ ANGMMAVAKK LGMAPRQLAE QVLTHLDLNG 
0071 IASKVEIAGP GFINIFLDPA FLAEHVQQAL ASDRLGVATP EKQTIVVDYS APNVAKEMHV GHLRSTIIGD 
0141 AAVRTLEFLG HKVIRANHVG DWGTQFGMLI AWLEKQQQEN AGEMELADLE GFYRDAKKHY DEDEEFAERA 
0211 RNYVVKLQSG DEYFREMWRK LVDITMTQNQ ITYDRLNVTL TRDDVMGESL YNPMLPGIVA DLKAKGLAVE 
0281 SEGATVVFLD EFKNKEGEPM GVIIQKKDGG YLYTTTDIAC AKYRYETLHA DRVLYYIDSR QHQHLMQAWA 
0351 IVRKAGYVPE SVPLEHHMFG MMLGKDGKPF KTRAGGTVKL ADLLDEALER ARRLVAEKNP DMPADELEKL 
0421 ANAVGIGAVK YADLSKNRTT DYIFDWDNML AFEGNTAPYM QYAYTRVLSV FRKAEIDEEQ LAAAPVIIRE 
0491 DREAQLAARL LQFEETLTVV AREGTPHVMC AYLYDLAGLF SGFYEHCPIL SAENEEVRNS RLKLAQLTAK 
0561 TLKLGLDTLG IETVERM

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             ACGTTGCTGA CGAAAACAGC CATTTGCAGT 
-070 ATACTCCCGC CCTAATTTCT TTAACTGGTG CGGGCAATTT TTGCTCGCTT CATCAATGTA AGGTATTCCG 
0001 gtgaatattc aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg cctgcggatt 
0071 gcgaaccgca ggttcgtcag tcagcaaaag ttcagttcgg cgactatcag gctaacggca tgatggcagt 
0141 tgctaaaaaa ctgggtatgg caccgcgaca attagcagag caggtgctga ctcatctgga tcttaacggt 
0211 atcgccagca aagttgagat cgccggtcca ggctttatca acattttcct tgatccggca ttcctggctg 
0281 aacatgttca gcaggcgctg gcgtccgatc gtctcggtgt tgctacgcca gaaaaacaga ccattgtggt 
0351 tgactactct gcgccaaacg tggcgaaaga gatgcatgtc ggtcacctgc gctctaccat tattggtgac 
0421 gcagcagtgc gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc gactggggca 
0491 ctcagttcgg tatgctgatt gcatggctgg aaaagcagca gcaggaaaac gccggtgaaa tggagctggc 
0561 tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac gatgaagatg aagagttcgc cgagcgcgca 
0631 cgtaactacg tggtaaaact gcaaagcggt gacgaatatt tccgcgagat gtggcgcaaa ctggtcgaca 
0701 tcaccatgac gcagaaccag atcacctacg atcgtctcaa cgtgacgctg acccgtgatg acgtgatggg 
0771 cgaaagcctc tacaacccga tgctgccagg aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa 
0841 agcgaagggg cgaccgtcgt attccttgat gagtttaaaa acaaggaagg cgaaccgatg ggcgtgatca 
0911 ttcagaagaa agatggcggc tatctctaca ccaccactga tatcgcctgt gcgaaatatc gttatgaaac 
0981 actgcatgcc gatcgcgtgc tgtattacat cgactcccgt cagcatcaac acctgatgca ggcatgggcg 
1051 atcgtccgta aagcaggcta tgtaccggaa tccgtaccgc tggaacacca catgttcggc atgatgctgg 
1121 gtaaagacgg caaaccgttc aaaacccgcg cgggtggtac agtgaaactg gccgatctgc tggatgaagc 
1191 cctggaacgt gcacgccgtc tggtggcaga aaagaacccg gatatgccag ccgacgagct ggaaaaactg 
1261 gctaacgcgg ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg gactacatct 
1331 tcgactggga caacatgctg gcgtttgagg gtaataccgc gccatacatg cagtatgcat acacgcgtgt 
1401 attgtccgtg ttccgtaaag cagaaattga cgaagagcaa ctggctgcag ctccggttat catccgtgaa 
1471 gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg aagaaaccct caccgtggtt gcccgtgaag 
1541 gcacgccgca tgtaatgtgt gcttacctgt acgatctggc cggtctgttc tctggcttct acgagcactg 
1611 cccgatcctc agcgcagaaa acgaagaagt gcgtaacagc cgtctaaaac tggcacaact gacggcgaag 
1681 acgctgaagc tgggtctgga tacgctgggt attgagactg tagagcgtat gtaaTCGATT TTTCGTGAGA 
1751 GTGAAGCCTG ATCAGGGTTA GCCGATCAGG CTTTTTTATT GCCATCTAAA TGTATTCTGA AAATGGACAT 
1821 GCCATTGTTT TCTC