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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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J Bacteriol. 1991;173(15):4799-813.
Cloning, mapping, and characterization of the Escherichia coli prc gene, which is involved in C-terminal processing of penicillin-binding protein 3.
Hara H, Yamamoto Y, Higashitani A, Suzuki H, Nishimura Y.
(
1856173
)
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Deletion
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OCL82
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
prc
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Related strains
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Strains Search
|
Search strains by gene name:
prc
Search strains by all related name:
prc b1830 ECK1829 JW1819 tsp
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General information (Go to Linear View: )
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Gene Name
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prc
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Alternative name
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b1830,ECK1829,JW1819,tsp
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Location, Length
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1,910,792 - 1,912,840
(
-
)
;
41.18
min
;
2049
(bp) ,
682
(aa)
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Product
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carboxy-terminal protease for penicillin-binding protein 3
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Operon Name
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proQ-prc
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Note
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GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_process: GO:0009252 - peptidoglycan biosynthetic process; GO_process: GO:0006457 - protein folding; GO_process: GO:0042493 - response to drug
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Function
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enzyme; Degradation of proteins, peptides, glyco
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Gene Ontology
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GO:0004252;
serine-type endopeptidase activity ( prc )
GO:0005515;
protein binding ( prc )
GO:0005886;
plasma membrane ( prc )
GO:0006508;
proteolysis ( prc )
GO:0008233;
peptidase activity ( prc )
GO:0008236;
serine-type peptidase activity ( prc )
GO:0016020;
membrane ( prc )
GO:0016787;
hydrolase activity ( prc )
GO:0019132;
C-terminal processing peptidase activity ( prc )
GO:0042597;
periplasmic space ( prc )
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PID
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1788134
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EC number
(KEGG Pathway)
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3.4.21.102
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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prc
(
EG10760
)
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Number of removed
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22 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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PRC_ECOLI
(
P23865
)
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- Protein name
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Tail-specific protease precursor
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- Synonyms
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EC 3.4.21.102, Protease Re, PRC protein, C-terminal processing peptidase
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- Gene name
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Name=prc; Synonyms=tsp; OrderedLocusNames=b1830;
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MMBR References
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J Bacteriol. 1989;171(11):5882-9.
Genetic analyses of processing involving C-terminal cleavage in penicillin-binding protein 3 of Escherichia coli.
Hara H, Nishimura Y, Kato J, Suzuki H, Nagasawa H, Suzuki A, Hirota Y.
(
2681145
)
|
J Bacteriol. 1991;173(15):4799-813.
Cloning, mapping, and characterization of the Escherichia coli prc gene, which is involved in C-terminal processing of penicillin-binding protein 3.
Hara H, Yamamoto Y, Higashitani A, Suzuki H, Nishimura Y.
(
1856173
)
|
J Bacteriol. 1994;176:6653-6662
use of transposon TnphoA to identify genes for cell envelope proteins of Escherichia coli required for long-chain fatty acid transport: the periplasmic protein Tsp potentiates long-chain fatty acid transport.
Azizan, A., and P. N. Black.
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Protein Sci. 1995;4:1507-1515
C-terminal specific protein degradation: activity and substrate specificity of the Tsp protease.
Keiler, K. C., K. R. Silber, K. M. Downard, I. A. Papayannopoulos, K. Biemann, and R. T. Sauer.
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J Bacteriol. 1989;171(11):5890-3.
Determination of the cleavage site involved in C-terminal processing of penicillin-binding protein 3 of Escherichia coli.
Nagasawa H, Sakagami Y, Suzuki A, Suzuki H, Hara H, Hirota Y.
(
2681146
)
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J Bacteriol. 1992;174:7844-7847
Multiple antibiotic susceptibility associated with inactivation of the prc gene.
Seoane, A. S., A. Sabbaj, L. M. McMurry, and S. B. Levy.
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Proc Natl Acad Sci U S A. 1992;89:295-299
Tsp: a tail-specific protease that selectively degrades proteins with nonpolar C termini.
Silber, K. R., K. C. Keiler, and R. T. Sauer.
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Mol Gen Genet. 1994;242(2):237-40.
Deletion of the prc (tsp) gene provides evidence for additional tail-specific proteolytic activity in Escherichia coli K-12.
Silber KR, Sauer RT.
(
8159175
)
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| Sequences |
Amino acid
FASTA format
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0001 MNMFFRLTAL AGLLAIAGQT FAVEDITRAD QIPVLKEETQ HATVSERVTS RFTRSHYRQF DLDQAFSAKI
0071 FDRYLNLLDY SHNVLLASDV EQFAKKKTEL GDELRSGKLD VFYDLYNLAQ KRRFERYQYA LSVLEKPMDF
0141 TGNDTYNLDR SKAPWPKNEA ELNALWDSKV KFDELSLKLT GKTDKEIRET LTRRYKFAIR RLAQTNSEDV
0211 FSLAMTAFAR EIDPHTNYLS PRNTEQFNTE MSLSLEGIGA VLQMDDDYTV INSMVAGGPA AKSKAISVGD
0281 KIVGVGQTGK PMVDVIGWRL DDVVALIKGP KGSKVRLEIL PAGKGTKTRT VTLTRERIRL EDRAVKMSVK
0351 TVGKEKVGVL DIPGFYVGLT DDVKVQLQKL EKQNVSSVII DLRSNGGGAL TEAVSLSGLF IPAGPIVQVR
0421 DNNGKVREDS DTDGQVFYKG PLVVLVDRFS ASASEIFAAA MQDYGRALVV GEPTFGKGTV QQYRSLNRIY
0491 DQMLRPEWPA LGSVQYTIQK FYRVNGGSTQ RKGVTPDIIM PTGNEETETG EKFEDNALPW DSIDAATYVK
0561 SGDLTAFEPE LLKEHNARIA KDPEFQNIMK DIARFNAMKD KRNIVSLNYA VREKENNEDD ATRLARLNER
0631 FKREGKPELK KLDDLPKDYQ EPDPYLDETV NIALDLAKLE KARPAEQPAP VK
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gaaatcacca aagacggcgt ccgcgtccag
-070 ctgaattcgg gtatgtcttt gattgtgcgc gcagaacacc tggtgttctg aaacggaggc cgggccaggc
0001 atgaacatgt tttttaggct taccgcgtta gctggcctgc ttgcaatagc aggccagacc ttcgctgtag
0071 aagatatcac gcgtgctgat caaattccgg tattaaagga agagacgcag catgcgacgg taagtgagcg
0141 cgtaacgtcg cgcttcaccc gttctcatta tcgccagttc gacctcgatc aggcattttc ggccaaaatc
0211 tttgaccgct acctgaatct gctcgattac agccacaacg tgctgctggc aagcgatgtt gaacagttcg
0281 cgaaaaagaa aaccgagtta ggcgatgaac tgcgttcagg caaactcgac gttttctacg atctctacaa
0351 tctggcgcaa aagcgccgtt ttgagcgtta ccagtacgct ttgtcggtac tggaaaagcc gatggatttc
0421 accggcaacg acacttataa ccttgaccgc agcaaagcgc cctggccgaa aaacgaggct gagttgaacg
0491 cgctgtggga cagtaaagtc aaattcgacg agttaagcct gaagctgaca ggaaaaacgg ataaagaaat
0561 tcgtgaaacc ctgactcgcc gctacaaatt tgccattcgt cgtctggcgc aaaccaacag cgaagatgtt
0631 ttctcgctgg caatgacggc gtttgcgcgt gaaatcgacc cgcataccaa ctatctttcc ccgcgtaata
0701 ccgaacagtt caacactgaa atgagtttgt cgctggaagg tattggcgca gtgctgcaaa tggatgatga
0771 ctacaccgtt atcaattcga tggtggcagg tggtccggca gcgaagagta aagctatcag cgttggtgac
0841 aaaattgtcg gtgttggtca aacaggcaag ccgatggttg acgtgattgg ctggcgtctt gatgatgtgg
0911 ttgccttaat taaagggccg aagggcagta aagttcgtct ggaaatttta cctgctggta aagggaccaa
0981 gacccgtact gtaacgttga cccgtgaacg tattcgtctc gaagaccgcg cggttaaaat gtcggtgaag
1051 accgtcggta aagagaaagt cggcgtgctg gatattccgg gcttctatgt gggtttgaca gacgatgtca
1121 aagtgcaact gcagaaactg gaaaaacaga atgtcagcag cgtcatcatc gacctgcgta gcaatggcgg
1191 tggggcgtta actgaagccg tatcgctctc cggtctgttt attcctgcgg gtcccattgt tcaggtccgc
1261 gataacaacg gcaaggttcg tgaagatagc gataccgacg gacaggtttt ctataaaggc ccgctggtgg
1331 tgctggttga ccgcttcagt gcttcggctt cagaaatctt tgccgcggca atgcaggatt acggtcgtgc
1401 gctggttgtg ggtgaaccga cgtttggtaa aggcaccgtt cagcaatacc gttcattgaa ccgtatttac
1471 gatcagatgt tacgtcctga atggccagcg ctgggttctg tgcagtacac gatccagaaa ttctatcgcg
1541 ttaacggcgg cagtacgcaa cgtaaaggcg taacgccaga catcatcatg ccgacgggta atgaagaaac
1611 ggaaacgggt gagaaattcg aagataacgc gctgccgtgg gatagcattg atgccgcgac ttatgtgaaa
1681 tcaggagatt taacggcctt tgaaccggag ctgctgaagg aacataatgc gcgtatcgcg aaagatcctg
1751 agttccagaa catcatgaag gatatcgcgc gcttcaacgc tatgaaggac aagcgcaata tcgtttctct
1821 gaattacgct gtgcgtgaga aagagaataa tgaagatgat gcgacgcgtc tggcgcgttt gaacgaacgc
1891 tttaaacgcg aaggtaaacc ggagttgaag aaactggatg atctaccgaa agattaccag gagccggatc
1961 cttatctgga tgagacggtg aatatcgcac tcgatctggc gaagcttgaa aaagccagac ccgcggaaca
2031 acccgctccc gtcaagtaat atcaatcagg cacaagaaat tgtgcctgat tttttaacag cgacaagatg
2101 ccgtaaatca gatgctacaa aatgtaaagt tgtgtctttc tggtgactt
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