MG1655
W3110
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Gene Report : prc
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
J Bacteriol. 1991;173(15):4799-813.
Cloning, mapping, and characterization of the Escherichia coli prc gene, which is involved in C-terminal processing of penicillin-binding protein 3.
Hara H, Yamamoto Y, Higashitani A, Suzuki H, Nishimura Y. ( 1856173 )
     Deletion OCL82 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  prc
Related strains
     Strains Search Search strains by gene name:  prc   Search strains by all related name:  prc b1830 ECK1829 JW1819 tsp

General information  (Go to Linear View:)
 Gene Name prc  
 Alternative name b1830,ECK1829,JW1819,tsp  
 Location, Length 1,910,792 - 1,912,840 (  -  ) ;   41.18 min ; 2049 (bp) ,   682 (aa) Go to Linear View
 Product carboxy-terminal protease for penicillin-binding protein 3  
 Operon Name proQ-prc  
 Note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_process: GO:0009252 - peptidoglycan biosynthetic process; GO_process: GO:0006457 - protein folding; GO_process: GO:0042493 - response to drug  
 Function enzyme; Degradation of proteins, peptides, glyco  
 Gene Ontology GO:0004252; serine-type endopeptidase activity ( prc )
GO:0005515; protein binding ( prc )
GO:0005886; plasma membrane ( prc )
GO:0006508; proteolysis ( prc )
GO:0008233; peptidase activity ( prc )
GO:0008236; serine-type peptidase activity ( prc )
GO:0016020; membrane ( prc )
GO:0016787; hydrolase activity ( prc )
GO:0019132; C-terminal processing peptidase activity ( prc )
GO:0042597; periplasmic space ( prc )
 PID 1788134  
 EC number
  (KEGG Pathway)
 
3.4.21.102  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene prc ( EG10760 )  
    Number of removed 22 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) PRC_ECOLI ( P23865 )
    -  Protein name Tail-specific protease precursor  
    -  Synonyms EC 3.4.21.102, Protease Re, PRC protein, C-terminal processing peptidase  
    -  Gene name Name=prc; Synonyms=tsp; OrderedLocusNames=b1830;  

 Linear View (Whole Mode)
View Location
   1900.0  –  1925.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP prc (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Photosystem Ii D1 C-Terminal Processing Protease  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Tail-specific protease precursor  
         PSI-BLAST Tail-specific protease precursor
    nr     (database updated : 2007.02.20 )
         BLAST carboxy-terminal protease for penicillin-binding protein 3 [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG0793M 
    EcoCyc prc 

MMBR References
J Bacteriol. 1989;171(11):5882-9.
Genetic analyses of processing involving C-terminal cleavage in penicillin-binding protein 3 of Escherichia coli.
Hara H, Nishimura Y, Kato J, Suzuki H, Nagasawa H, Suzuki A, Hirota Y. ( 2681145 )
J Bacteriol. 1991;173(15):4799-813.
Cloning, mapping, and characterization of the Escherichia coli prc gene, which is involved in C-terminal processing of penicillin-binding protein 3.
Hara H, Yamamoto Y, Higashitani A, Suzuki H, Nishimura Y. ( 1856173 )
J Bacteriol. 1994;176:6653-6662
use of transposon TnphoA to identify genes for cell envelope proteins of Escherichia coli required for long-chain fatty acid transport: the periplasmic protein Tsp potentiates long-chain fatty acid transport.
Azizan, A., and P. N. Black.
Protein Sci. 1995;4:1507-1515
C-terminal specific protein degradation: activity and substrate specificity of the Tsp protease.
Keiler, K. C., K. R. Silber, K. M. Downard, I. A. Papayannopoulos, K. Biemann, and R. T. Sauer.
J Bacteriol. 1989;171(11):5890-3.
Determination of the cleavage site involved in C-terminal processing of penicillin-binding protein 3 of Escherichia coli.
Nagasawa H, Sakagami Y, Suzuki A, Suzuki H, Hara H, Hirota Y. ( 2681146 )
J Bacteriol. 1992;174:7844-7847
Multiple antibiotic susceptibility associated with inactivation of the prc gene.
Seoane, A. S., A. Sabbaj, L. M. McMurry, and S. B. Levy.
Proc Natl Acad Sci U S A. 1992;89:295-299
Tsp: a tail-specific protease that selectively degrades proteins with nonpolar C termini.
Silber, K. R., K. C. Keiler, and R. T. Sauer.
Mol Gen Genet. 1994;242(2):237-40.
Deletion of the prc (tsp) gene provides evidence for additional tail-specific proteolytic activity in Escherichia coli K-12.
Silber KR, Sauer RT. ( 8159175 )

Sequences
Amino acid
FASTA format
0001 MNMFFRLTAL AGLLAIAGQT FAVEDITRAD QIPVLKEETQ HATVSERVTS RFTRSHYRQF DLDQAFSAKI 
0071 FDRYLNLLDY SHNVLLASDV EQFAKKKTEL GDELRSGKLD VFYDLYNLAQ KRRFERYQYA LSVLEKPMDF 
0141 TGNDTYNLDR SKAPWPKNEA ELNALWDSKV KFDELSLKLT GKTDKEIRET LTRRYKFAIR RLAQTNSEDV 
0211 FSLAMTAFAR EIDPHTNYLS PRNTEQFNTE MSLSLEGIGA VLQMDDDYTV INSMVAGGPA AKSKAISVGD 
0281 KIVGVGQTGK PMVDVIGWRL DDVVALIKGP KGSKVRLEIL PAGKGTKTRT VTLTRERIRL EDRAVKMSVK 
0351 TVGKEKVGVL DIPGFYVGLT DDVKVQLQKL EKQNVSSVII DLRSNGGGAL TEAVSLSGLF IPAGPIVQVR 
0421 DNNGKVREDS DTDGQVFYKG PLVVLVDRFS ASASEIFAAA MQDYGRALVV GEPTFGKGTV QQYRSLNRIY 
0491 DQMLRPEWPA LGSVQYTIQK FYRVNGGSTQ RKGVTPDIIM PTGNEETETG EKFEDNALPW DSIDAATYVK 
0561 SGDLTAFEPE LLKEHNARIA KDPEFQNIMK DIARFNAMKD KRNIVSLNYA VREKENNEDD ATRLARLNER 
0631 FKREGKPELK KLDDLPKDYQ EPDPYLDETV NIALDLAKLE KARPAEQPAP VK

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             gaaatcacca aagacggcgt ccgcgtccag 
-070 ctgaattcgg gtatgtcttt gattgtgcgc gcagaacacc tggtgttctg aaacggaggc cgggccaggc 
0001 atgaacatgt tttttaggct taccgcgtta gctggcctgc ttgcaatagc aggccagacc ttcgctgtag 
0071 aagatatcac gcgtgctgat caaattccgg tattaaagga agagacgcag catgcgacgg taagtgagcg 
0141 cgtaacgtcg cgcttcaccc gttctcatta tcgccagttc gacctcgatc aggcattttc ggccaaaatc 
0211 tttgaccgct acctgaatct gctcgattac agccacaacg tgctgctggc aagcgatgtt gaacagttcg 
0281 cgaaaaagaa aaccgagtta ggcgatgaac tgcgttcagg caaactcgac gttttctacg atctctacaa 
0351 tctggcgcaa aagcgccgtt ttgagcgtta ccagtacgct ttgtcggtac tggaaaagcc gatggatttc 
0421 accggcaacg acacttataa ccttgaccgc agcaaagcgc cctggccgaa aaacgaggct gagttgaacg 
0491 cgctgtggga cagtaaagtc aaattcgacg agttaagcct gaagctgaca ggaaaaacgg ataaagaaat 
0561 tcgtgaaacc ctgactcgcc gctacaaatt tgccattcgt cgtctggcgc aaaccaacag cgaagatgtt 
0631 ttctcgctgg caatgacggc gtttgcgcgt gaaatcgacc cgcataccaa ctatctttcc ccgcgtaata 
0701 ccgaacagtt caacactgaa atgagtttgt cgctggaagg tattggcgca gtgctgcaaa tggatgatga 
0771 ctacaccgtt atcaattcga tggtggcagg tggtccggca gcgaagagta aagctatcag cgttggtgac 
0841 aaaattgtcg gtgttggtca aacaggcaag ccgatggttg acgtgattgg ctggcgtctt gatgatgtgg 
0911 ttgccttaat taaagggccg aagggcagta aagttcgtct ggaaatttta cctgctggta aagggaccaa 
0981 gacccgtact gtaacgttga cccgtgaacg tattcgtctc gaagaccgcg cggttaaaat gtcggtgaag 
1051 accgtcggta aagagaaagt cggcgtgctg gatattccgg gcttctatgt gggtttgaca gacgatgtca 
1121 aagtgcaact gcagaaactg gaaaaacaga atgtcagcag cgtcatcatc gacctgcgta gcaatggcgg 
1191 tggggcgtta actgaagccg tatcgctctc cggtctgttt attcctgcgg gtcccattgt tcaggtccgc 
1261 gataacaacg gcaaggttcg tgaagatagc gataccgacg gacaggtttt ctataaaggc ccgctggtgg 
1331 tgctggttga ccgcttcagt gcttcggctt cagaaatctt tgccgcggca atgcaggatt acggtcgtgc 
1401 gctggttgtg ggtgaaccga cgtttggtaa aggcaccgtt cagcaatacc gttcattgaa ccgtatttac 
1471 gatcagatgt tacgtcctga atggccagcg ctgggttctg tgcagtacac gatccagaaa ttctatcgcg 
1541 ttaacggcgg cagtacgcaa cgtaaaggcg taacgccaga catcatcatg ccgacgggta atgaagaaac 
1611 ggaaacgggt gagaaattcg aagataacgc gctgccgtgg gatagcattg atgccgcgac ttatgtgaaa 
1681 tcaggagatt taacggcctt tgaaccggag ctgctgaagg aacataatgc gcgtatcgcg aaagatcctg 
1751 agttccagaa catcatgaag gatatcgcgc gcttcaacgc tatgaaggac aagcgcaata tcgtttctct 
1821 gaattacgct gtgcgtgaga aagagaataa tgaagatgat gcgacgcgtc tggcgcgttt gaacgaacgc 
1891 tttaaacgcg aaggtaaacc ggagttgaag aaactggatg atctaccgaa agattaccag gagccggatc 
1961 cttatctgga tgagacggtg aatatcgcac tcgatctggc gaagcttgaa aaagccagac ccgcggaaca 
2031 acccgctccc gtcaagtaat atcaatcagg cacaagaaat tgtgcctgat tttttaacag cgacaagatg 
2101 ccgtaaatca gatgctacaa aatgtaaagt tgtgtctttc tggtgactt