MG1655
W3110
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Gene Report : fadD
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Biochim Biophys Acta. 1990;1046(1):97-105.
Characterization of FadL-specific fatty acid binding in Escherichia coli.
Black PN. ( 2204431 )
     Deletion OCL83 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  fadD
Related strains
     Strains Search Search strains by gene name:  fadD   Search strains by all related name:  fadD b1805 ECK1803 JW1794 oldD

General information  (Go to Linear View:)
 Gene Name fadD  
 Alternative name b1805,ECK1803,JW1794,oldD  
 Location, Length 1,886,085 - 1,887,770 (  -  ) ;   40.65 min ; 1686 (bp) ,   561 (aa) Go to Linear View
 Product acyl-CoA synthetase (long-chain-fatty-acid--CoA ligase)  
 Operon Name fadD-sroD  
 Note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_process: GO:0019395 - fatty acid oxidation; GO_process: GO:0008654 - phospholipid biosynthetic process  
 Function enzyme; Degradation of small molecules: Fatty acids  
 Gene Ontology GO:0000166 ; nucleotide binding ( fadD )
GO:0003824 ; catalytic activity ( fadD )
GO:0004467 ; long-chain-fatty-acid-CoA ligase activity ( fadD )
GO:0005524 ; ATP binding ( fadD )
GO:0006629 ; lipid metabolic process ( fadD )
GO:0006631 ; fatty acid metabolic process ( fadD )
GO:0008152 ; metabolic process ( fadD )
GO:0016020 ; membrane ( fadD )
GO:0016874 ; ligase activity ( fadD )
 PID 1788107  
 EC number
  (KEGG Pathway)   		6.2.1.3  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene fadD ( EG11530 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) LCFA_ECOLI ( P29212 )
    -  Protein name Long-chain-fatty-acid--CoA ligase  
    -  Synonyms EC 6.2.1.3, Long-chain acyl-CoA synthetase  
    -  Gene name Name=fadD; Synonyms=oldD; OrderedLocusNames=b1805, c2209;  

 Linear View (Whole Mode)
View Location
   1875.0  –  1900.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP fadD (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of The Thermostable Japanese Firefly Luciferase Complexed With High-Energy Intermediate Analogue  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Long-chain-fatty-acid--CoA ligase (Long-chain acyl-CoA synthetase) (Acyl-CoA synthetase)  
         PSI-BLAST Long-chain-fatty-acid--CoA ligase (Long-chain acyl-CoA synthetase) (Acyl-CoA synthetase)
    nr     (database updated : 2007.02.20 )
         BLAST acyl-CoA synthase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    HELIX_LOOP_HELIX    MICROBODIES_CTER    AMP_BINDING     

Other Cross-Reference
    COG COG0318IQ 
    EcoCyc fadD 

MMBR References
Mol Gen Genet. 1994;242(3):241-9.
The fadD gene of Escherichia coli K12 is located close to rnd at 39.6 min of the chromosomal map and is a new member of the AMP-binding protein family.
Fulda M, Heinz E, Wolter FP. ( 8107670 )
J Biol Chem. 1992;267:25513-25520
Cloning, sequencing, and expression of the fadD gene of Escherichia coli encoding acyl coenzyme A synthetase.
Black, P. N., C. C. DiRusso, A. K. Metzger, and T. L. Heimert.

Sequences
Amino acid
FASTA format 
0001 MKKVWLNRYP ADVPTEINPD RYQSLVDMFE QSVARYADQP AFVNMGEVMT FRKLEERSRA FAAYLQQGLG 
0071 LKKGDRVALM MPNLLQYPVA LFGILRAGMI VVNVNPLYTP RELEHQLNDS GASAIVIVSN FAHTLEKVVD 
0141 KTAVQHVILT RMGDQLSTAK GTVVNFVVKY IKRLVPKYHL PDAISFRSAL HNGYRMQYVK PELVPEDLAF 
0211 LQYTGGTTGV AKGAMLTHRN MLANLEQVNA TYGPLLHPGK ELVVTALPLY HIFALTINCL LFIELGGQNL 
0281 LITNPRDIPG LVKELAKYPF TAITGVNTLF NALLNNKEFQ QLDFSSLHLS AGGGMPVQQV VAERWVKLTG 
0351 QYLLEGYGLT ECAPLVSVNP YDIDYHSGSI GLPVPSTEAK LVDDDDNEVP PGQPGELCVK GPQVMLGYWQ 
0421 RPDATDEIIK NGWLHTGDIA VMDEEGFLRI VDRKKDMILV SGFNVYPNEI EDVVMQHPGV QEVAAVGVPS 
0491 GSSGEAVKIF VVKKDPSLTE ESLVTFCRRQ LTGYKVPKLV EFRDELPKSN VGKILRRELR DEARGKVDNK 
0561 A
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             ggtaattatc aagctggtat gatgagttaa 
-070 tattatgtta acggcatgta tatcatttgg ggttgcgatg acgacgaaca cgcattttag aggtgaagaa 
0001 ttgaagaagg tttggcttaa ccgttatccc gcggacgttc cgacggagat caaccctgac cgttatcaat 
0071 ctctggtaga tatgtttgag cagtcggtcg cgcgctacgc cgatcaacct gcgtttgtga atatggggga 
0141 ggtaatgacc ttccgcaagc tggaagaacg cagtcgcgcg tttgccgctt atttgcaaca agggttgggg 
0211 ctgaagaaag gcgatcgcgt tgcgttgatg atgcctaatt tattgcaata tccggtggcg ctgtttggca 
0281 ttttgcgtgc cgggatgatc gtcgtaaacg ttaacccgtt gtataccccg cgtgagcttg agcatcagct 
0351 taacgatagc ggcgcatcgg cgattgttat cgtgtctaac tttgctcaca cactggaaaa agtggttgat 
0421 aaaaccgccg ttcagcacgt aattctgacc cgtatgggcg atcagctatc tacggcaaaa ggcacggtag 
0491 tcaatttcgt tgttaaatac atcaagcgtt tggtgccgaa ataccatctg ccagatgcca tttcatttcg 
0561 tagcgcactg cataacggct accggatgca gtacgtcaaa cccgaactgg tgccggaaga tttagctttt 
0631 ctgcaataca ccggcggcac cactggtgtg gcgaaaggcg cgatgctgac tcaccgcaat atgctggcga 
0701 acctggaaca ggttaacgcg acctatggtc cgctgttgca tccgggcaaa gagctggtgg tgacggcgct 
0771 gccgctgtat cacatttttg ccctgaccat taactgcctg ctgtttatcg aactgggtgg gcagaacctg 
0841 cttatcacta acccgcgcga tattccaggg ttggtaaaag agttagcgaa atatccgttt accgctatca 
0911 cgggcgttaa caccttgttc aatgcgttgc tgaacaataa agagttccag cagctggatt tctccagtct 
0981 gcatctttcc gcaggcggtg ggatgccagt gcagcaagtg gtggcagagc gttgggtgaa actgaccgga 
1051 cagtatctgc tggaaggcta tggccttacc gagtgtgcgc cgctggtcag cgttaaccca tatgatattg 
1121 attatcatag tggtagcatc ggtttgccgg tgccgtcgac ggaagccaaa ctggtggatg atgatgataa 
1191 tgaagtacca ccaggtcaac cgggtgagct ttgtgtcaaa ggaccgcagg tgatgctggg ttactggcag 
1261 cgtcccgatg ctaccgatga aatcatcaaa aatggctggt tacacaccgg cgacatcgcg gtaatggatg 
1331 aagaaggatt cctgcgcatt gtcgatcgta aaaaagacat gattctggtt tccggtttta acgtctatcc 
1401 caacgagatt gaagatgtcg tcatgcagca tcctggcgta caggaagtcg cggctgttgg cgtaccttcc 
1471 ggctccagtg gtgaagcggt gaaaatcttc gtagtgaaaa aagatccatc gcttaccgaa gagtcactgg 
1541 tgactttttg ccgccgtcag ctcacgggat acaaagtacc gaagctggtg gagtttcgtg atgagttacc 
1611 gaaatctaac gtcggaaaaa ttttgcgacg agaattacgt gacgaagcgc gcggcaaagt ggacaataaa 
1681 gcctgagcgt taagtcagtc gtcagacgcc ggttaatccg gcgttttttt tgacgcccac taaagagaaa 
1751 acaatttgaa ttaccaaatg attaccacgg acgatg