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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
|
OCL85
(D)
|
|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
chbF
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Related strains
|
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Strains Search
|
Search strains by gene name:
chbF
Search strains by all related name:
chbF b1734 celF ECK1732 f450 JW1723 ydjD
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General information (Go to Linear View: )
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Gene Name
|
chbF
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Alternative name
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b1734,celF,ECK1732,f450,JW1723,ydjD
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Location, Length
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1,815,172 - 1,816,524
(
-
)
;
39.12
min
;
1353
(bp) ,
450
(aa)
|
|
Product
|
phospho-chitobiase; general 6-phospho-beta-glucosidase activity
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Operon Name
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chbBCARFG
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Note
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GO_component: GO:0005737 - cytoplasm; GO_process: GO:0016052 - carbohydrate catabolic process; GO_process: GO:0009435 - NAD biosynthetic process
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Function
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enzyme; Degradation of small molecules: N,N'-diacetylchitobiose
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Gene Ontology
|
GO:0003824
;
catalytic activity ( chbF )
GO:0004553
;
hydrolase activity, hydrolyzing O-glycosyl compounds ( chbF )
GO:0005488
;
binding ( chbF )
GO:0005975
;
carbohydrate metabolic process ( chbF )
GO:0008152
;
metabolic process ( chbF )
GO:0008706
;
6-phospho-beta-glucosidase activity ( chbF )
GO:0016151
;
nickel ion binding ( chbF )
GO:0016787
;
hydrolase activity ( chbF )
GO:0016798
;
hydrolase activity, acting on glycosyl bonds ( chbF )
GO:0030145
;
manganese ion binding ( chbF )
GO:0046872
;
metal ion binding ( chbF )
GO:0050897
;
cobalt ion binding ( chbF )
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PID
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1788029
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EC number
(KEGG Pathway)
|
3.2.1.86
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
|
chbF
(
EG10144
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
|
CHBF_ECOLI
(
P17411
;
P37795; P78290
)
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- Protein name
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6-phospho-beta-glucosidase
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- Synonyms
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EC 3.2.1.86, Cellobiose-6-phosphate hydrolase, Phospho-chitobiase
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- Gene name
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Name=chbF; Synonyms=celF; OrderedLocusNames=b1734;
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MMBR References
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Proc Natl Acad Sci U S A. 1997;94:14367-14371
Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-diacetylchitobiose, by expressing the cel operon.
Keyhani, N. O., and S. Roseman.
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Mol Biol Evol. 1984;1:171-182
Directed evolution of cellobiose utilization in Escherichia coli K12.
Kricker, M., and B. G. Hall.
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Genetics 1987;115:419-429
Biochemical genetics of the cryptic gene system for cellobiose utilization in Escherichia coli K12.
Kricker, M., and B. G. Hall.
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Genetics 1990;124:455-471
Characterization and nucleotide sequence of the cryptic cel operon of E. coli K12.
Parker, L. L., and B. G. Hall.
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| Sequences |
Amino acid
FASTA format
|
0001 MSQKLKVVTI GGGSSYTPEL LEGFIKRYHE LPVSELWLVD VEGGKPKLDI IFDLCQRMID NAGVPMKLYK
0071 TLDRREALKD ADFVTTQLRV GQLPARELDE RIPLSHGYLG QETNGAGGLF KGLRTIPVIF DIVKDVEELC
0141 PNAWVINFTN PAGMVTEAVY RHTGFKRFIG VCNIPIGMKM FIRDVLMLKD SDDLSIDLFG LNHMVFIKDV
0211 LINGKSRFAE LLDGVASGQL KASSVKNIFD LPFSEGLIRS LNLLPCSYLL YYFKQKEMLA IEMGEYYKGG
0281 ARAQVVQKVE KQLFELYKNP ELKVKPKELE QRGGAYYSDA ACEVINAIYN DKQAEHYVNI PHHGQIDNIP
0351 ADWAVEMTCK LGRDGATPHP RITHFDDKVM GLIHTIKGFE IAASNAALSG EFNDVLLALN LSPLVHSDRD
0421 AELLAREMIL AHEKWLPNFA DCIAELKKAH
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ttatacctga caattcacat atctgtcctg
-070 ttgctggatt atttatgtcc gggggggcag atatgccaga tatcagtatt ctgtactgaa gggagaaatt
0001 atgagccaga aattaaaagt cgtcactatt ggtggcggga gcagctatac cccggagtta ctggaaggat
0071 ttattaagcg ttatcacgaa ttgccggtca gcgaattatg gctggtggat gtcgaaggtg gtaaaccgaa
0141 actggatatt attttcgatc tctgccaacg gatgattgat aacgctggcg tcccgatgaa gctttataaa
0211 acgctggatc gccgcgaagc attgaaagat gctgatttcg ttactaccca actgcgcgtt ggccaattac
0281 cggcgcgtga actggatgaa cgtattccat taagtcatgg ttatcttggt caggaaacca acggcgcggg
0351 cggtttgttt aaaggtctgc gtaccattcc ggtgattttt gacatcgtaa aagatgtcga agaactttgt
0421 ccgaatgcat gggtgattaa cttcactaac ccggcgggaa tggtcactga agccgtttat cgtcataccg
0491 gatttaaacg ctttatcggc gtgtgtaata ttccgatcgg catgaagatg tttattcgcg atgttctgat
0561 gctgaaagac agcgatgatt tatctatcga tttgttcggc ctcaaccata tggtgttcat taaggatgtg
0631 ctgataaatg gcaagtcgcg ctttgccgaa ttgcttgatg gtgtggcgtc agggcagtta aaagcgtcct
0701 ctgtaaaaaa tattttcgat ctgccattta gtgagggctt aattcgttcg ttgaatctgc tgccatgttc
0771 ttatctgctg tattacttca agcagaaaga gatgctggct attgaaatgg gcgaatacta caaaggcggc
0841 gcacgagcgc aggtagtaca gaaagtcgag aaacaacttt ttgagctgta taaaaatcct gagctgaaag
0911 ttaagccgaa agaactggaa cagcgcggtg gggcttatta ctctgatgca gcatgcgaag tgatcaacgc
0981 tatctacaac gacaagcaag cagaacatta cgttaatatc ccgcatcatg ggcagattga taatattccg
1051 gcagactggg cagtagaaat gacctgtaag ctggggcgcg atggcgcgac gccacatccg cgcattacgc
1121 atttcgatga taaagtaatg gggctgattc acaccattaa aggcttcgag attgctgcca gtaacgccgc
1191 acttagcgga gaatttaacg atgtgttact ggcgctaaac cttagtccgt tggtgcattc cgatcgcgat
1261 gctgagctgc tggcacgcga gatgattctg gcgcacgaga aatggctgcc aaactttgcc gactgcatcg
1331 cagagcttaa aaaagcacat taaccgaggc tgattatgga acgcttactg attgttaatg ccgatgattt
1401 tggcttaagc aaaggtcaga actacggcat tatcgaggcc tgtcgcaatg gga
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