MG1655
W3110
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Gene Report : tsf
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)  
     Deletion OCR33,34-1 (D)  ,  OCR33,34-2 (D)  ,  OCR33,35-4 (AK4-2 / BK5-2) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  tsf
Related strains
     Strains Search Search strains by gene name:  tsf   Search strains by all related name:  tsf b0170 ECK0169 JW0165

General information  (Go to Linear View:)
 Gene Name tsf  
 Alternative name b0170,ECK0169,JW0165  
 Location, Length 190,857 - 191,708 (  +  ) ;   4.11 min ; 852 (bp) ,   283 (aa) Go to Linear View
 Product protein chain elongation factor EF-Ts  
 Operon Name tff-rpsB-tsf  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006412 - translation  
 Function factor; Proteins - translation and modification  
 Gene Ontology GO:0003746; translation elongation factor activity ( tsf )
GO:0005515; protein binding ( tsf )
GO:0005622; intracellular ( tsf )
GO:0005624; membrane fraction ( tsf )
GO:0005737; cytoplasm ( tsf )
GO:0006412; translation ( tsf )
GO:0006414; translational elongation ( tsf )
 PID 1786366  
 EC number
  (KEGG Pathway)
 
 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene tsf ( EG11033 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) EFTS_ECOLI ( P02997 )
    -  Protein name Elongation factor Ts  
    -  Synonyms EF-Ts  
    -  Gene name OrderedLocusNames=b0170, c0206, z0181, ECs0172, SF0160, S0163;  

 Linear View (Whole Mode)
View Location
   180.0  –  205.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP tsf (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain B, Elongation Factor Complex Ef-TuEF-Ts From Escherichia Coli  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Elongation factor Ts (EF-Ts)  
         PSI-BLAST Elongation factor Ts (EF-Ts)
    nr     (database updated : 2007.02.20 )
         BLAST elongation factor Ts [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    EF_TS_1    EF_TS_2     

Other Cross-Reference
    EcoCyc tsf 

MMBR References
Mol Gen Genet. 1981;182(1):178-9.
Revised location of the Escherichia coli gene coding for ribosomal protein S2.
Lathe R, Bollen A, Herzog R. ( 7022135 )
Mol Gen Genet. 1981;181(3):356-62.
Organization of genes in the four minute region of the Escherichia coli chromosome: evidence that rpsB and tsf are co-transcribed.
Bendiak DS, Friesen JD. ( 7017348 )
J Mol Biol. 1979;132(2):219-33.
A conditionally lethal mutation of Escherichia coli affecting the gene coding for ribosomal protein S2 (rpsB).
Bollen A, Lathe R, Herzog A, Denicourt D, Lecocq JP, Desmarez L, Lavalle R. ( 161328 )
Nucleic Acids Res. 1981;9:4163-4172
Organization and nucleotide sequence of a new ribosomal operon in Escherichia coli containing the genes for ribosomal protein S2 and elongation factor Ts.
An, G., D. S. Bendiak, L. A. Mamelak, and J. D. Friesen.

Sequences
Amino acid
FASTA format
0001 MAEITASLVK ELRERTGAGM MDCKKALTEA NGDIELAIEN MRKSGAIKAA KKAGNVAADG VIKTKIDGNY 
0071 GIILEVNCQT DFVAKDAGFQ AFADKVLDAA VAGKITDVEV LKAQFEEERV ALVAKIGENI NIRRVAALEG 
0141 DVLGSYQHGA RIGVLVAAKG ADEELVKHIA MHVAASKPEF IKPEDVSAEV VEKEYQVQLD IAMQSGKPKE 
0211 IAEKMVEGRM KKFTGEVSLT GQPFVMEPSK TVGQLLKEHN AEVTGFIRFE VGEGIEKVET DFAAEVAAMS 
0281 KQS

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             TAGTACACGT TTGGTTAGGG GGCCTGCATA 
-070 TGGCCCCCTT TTTCACTTTT ATATCTGTGC GGTTTAATGC CGGGCAGATC ACATCTCCGA GGATTTTAGA 
0001 atggctgaaa ttaccgcatc cctggtaaaa gagctgcgtg agcgtactgg cgcaggcatg atggattgca 
0071 aaaaagcact gactgaagct aacggcgaca tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat 
0141 taaagcagcg aaaaaagcag gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac 
0211 ggcatcattc tggaagttaa ctgccagact gacttcgttg caaaagacgc tggtttccag gcgttcgcag 
0281 acaaagttct ggacgcagct gttgctggca aaatcactga cgttgaagtt ctgaaagcac agttcgaaga 
0351 agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc aacattcgcc gcgttgctgc gctggaaggc 
0421 gacgttctgg gttcttatca gcacggtgcg cgtatcggcg ttctggttgc tgctaaaggc gctgacgaag 
0491 agctggttaa acacatcgct atgcacgttg ctgcaagcaa gccagaattc atcaaaccgg aagacgtatc 
0561 cgctgaagtg gtagaaaaag aataccaggt acagctggat atcgcgatgc agtctggtaa gccgaaagaa 
0631 atcgcagaga aaatggttga aggccgcatg aagaaattca ccggcgaagt ttctctgacc ggtcagccgt 
0701 tcgttatgga accaagcaaa actgttggtc agctgctgaa agagcataac gctgaagtga ctggcttcat 
0771 ccgcttcgaa gtgggtgaag gcatcgagaa agttgagact gactttgcag cagaagttgc tgcgatgtcc 
0841 aagcagtctt aaTTATCAAA AAGGAGCCGC CTGAGGGCGG CTTCTTTTTG TGCCCATCTT GTAAATTCAG 
0911 CTAACCCTTG TGGGGCTGCG CTGAAAAGCG ACGTACAATG TC