MG1655
W3110
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Gene Report : thrS
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
J Mol Biol. 1985;185(1):93-104.
Autogenous control of Escherichia coli threonyl-tRNA synthetase expression in vivo.
Springer M, Plumbridge JA, Butler JS, Graffe M, Dondon J, Mayaux JF, Fayat G, Lestienne P, Blanquet S, Grunberg-Manago M. ( 3930755 )
     Deletion OCL85,86-8 (AK4-4 / BK6-4) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  thrS
Related strains
     Strains Search Search strains by gene name:  thrS   Search strains by all related name:  thrS b1719 ECK1717 JW1709

General information  (Go to Linear View:)
 Gene Name thrS  
 Alternative name b1719,ECK1717,JW1709  
 Location, Length 1,798,666 - 1,800,594 (  -  ) ;   38.77 min ; 1929 (bp) ,   642 (aa) Go to Linear View
 Product threonyl-tRNA synthetase  
 Operon Name thrS-infC-rpmI-rplT-pheMST-ihfA  
 Note threonine tRNA synthetase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006418 - tRNA aminoacylation for protein translation  
 Function enzyme; Aminoacyl tRNA synthetases, tRNA modification  
 Gene Ontology GO:0000166; nucleotide binding ( thrS )
GO:0003723; RNA binding ( thrS )
GO:0004812; aminoacyl-tRNA ligase activity ( thrS )
GO:0004829; threonine-tRNA ligase activity ( thrS )
GO:0005515; protein binding ( thrS )
GO:0005524; ATP binding ( thrS )
GO:0005737; cytoplasm ( thrS )
GO:0006412; translation ( thrS )
GO:0006417; regulation of translation ( thrS )
GO:0006418; tRNA aminoacylation for protein translation ( thrS )
GO:0006435; threonyl-tRNA aminoacylation ( thrS )
GO:0008270; zinc ion binding ( thrS )
GO:0016874; ligase activity ( thrS )
GO:0016876; ligase activity, forming aminoacyl-tRNA and related compounds ( thrS )
GO:0043039; tRNA aminoacylation ( thrS )
GO:0046872; metal ion binding ( thrS )
 PID 1788013  
 EC number
  (KEGG Pathway)
 
6.1.1.3  
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) SYT_ECOLI ( P00955 ; P78166; P78241 )
    -  Protein name Threonyl-tRNA synthetase  
    -  Synonyms EC 6.1.1.3, Threonine--tRNA ligase, ThrRS  
    -  Gene name Name=thrS; OrderedLocusNames=b1719, c2116, SF1512, S1630;  

 Linear View (Whole Mode)
View Location
   1790.0  –  1815.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP thrS (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Structure Of E. Coli Threonyl-Trna Synthetase Complexed With Its Cognate Trna  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Threonyl-tRNA synthetase (Threonine--tRNA ligase) (ThrRS)  
         PSI-BLAST Threonyl-tRNA synthetase (Threonine--tRNA ligase) (ThrRS)
    nr     (database updated : 2007.02.20 )
         BLAST threonyl-tRNA synthetase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    RGD    AA_TRNA_LIGASE_II_1    AA_TRNA_LIGASE_II_2     

Other Cross-Reference
    COG COG0441J 
    EcoCyc thrS 

MMBR References
J Bacteriol. 1980;143(3):1135-41.
Genetic analysis of mutations causing borrelidin resistance by overproduction of threonyl-transfer ribonucleic acid synthetase.
Frohler J, Rechenmacher A, Thomale J, Nass G, Bock A. ( 6997265 )
J Bacteriol. 1993;175(2):307-16.
Autogenous regulation of gene expression.
Maloy S, Stewart V. ( 8419283 )
Proc Natl Acad Sci U S A. 1983;80:6152-6156
Structural and transcriptional evidence for related thrS and infC expression.
Mayaux, J.-F., G. Fayat, M. Fromant, M. Springer, and M. Grunberg-Manago.
FEBS Lett. 1974;39(2):182-6.
Alteration of structure of level of threonyl-tRNA-synthetase in Borrelidin resistant mutants of E. coli.
Nass G, Thomale J. ( 4368395 )
Eur J Biochem. 1973;35(2):331-7.
Biochemical and immunological characterization of threonyl-tRNA synthetase of two borrelidin-resistant mutants of Escherichia coli K12.
Paetz W, Nass G. ( 4577856 )
J Bacteriol. 1982;152(2):661-8.
Escherichia coli phenylalanyl-tRNA synthetase operon: transcription studies of wild-type and mutated operons on multicopy plasmids.
Plumbridge JA, Springer M. ( 6215395 )
Gene. 1980;11(1-2):33-42.
Physical localisation and cloning of the structural gene for E. coli initiation factor IF3 from a group of genes concerned with translation.
Plumbridge JA, Springer M, Graffe M, Goursot R, Grunberg-Manago M. ( 6254853 )
J Bacteriol. 1986;168(2):746-51.
Expression of Escherichia coli infC: identification of a promoter in an upstream thrS coding sequence.
Pramanik A, Wertheimer SJ, Schwartz JJ, Schwartz I. ( 3536858 )
Mol Gen Genet. 1982;186(2):247-52.
Transcription units around the gene for E. coli translation initiation factor IF3 (infC).
Springer M, Plumbridge JA, Trudel M, Graffe M, Grunberg-Manago M. ( 7050631 )
Proc Natl Acad Sci U S A. 1986;83:4384-4388
Genetic definition of the translational operator of the threonine-tRNA ligase gene in Escherichia coli.
Springer, M., M. Graffe, J. S. Butler, and M. Grunberg-Manago.
Gene. 1988;63(2):309-20.
Transcriptional patterns for the thrS-infC-rplT operon of Escherichia coli.
Wertheimer SJ, Klotsky RA, Schwartz I. ( 2838394 )
J Mol Biol. 1984;173(2):177-209.
Transcription of a gene cluster coding for two aminoacyl-tRNA synthetases and an initiation factor in Escherichia coli.
Wu TH, Wood DL, Stein PL, Comer MM. ( 6368838 )

Sequences
Amino acid
FASTA format
0001 MPVITLPDGS QRHYDHAVSP MDVALDIGPG LAKACIAGRV NGELVDACDL IENDAQLSII TAKDEEGLEI 
0071 IRHSCAHLLG HAIKQLWPHT KMAIGPVIDN GFYYDVDLDR TLTQEDVEAL EKRMHELAEK NYDVIKKKVS 
0141 WHEARETFAN RGESYKVSIL DENIAHDDKP GLYFHEEYVD MCRGPHVPNM RFCHHFKLMK TAGAYWRGDS 
0211 NNKMLQRIYG TAWADKKALN AYLQRLEEAA KRDHRKIGKQ LDLYHMQEEA PGMVFWHNDG WTIFRELEVF 
0281 VRSKLKEYQY QEVKGPFMMD RVLWEKTGHW DNYKDAMFTT SSENREYCIK PMNCPGHVQI FNQGLKSYRD 
0351 LPLRMAEFGS CHRNEPSGSL HGLMRVRGFT QDDAHIFCTE EQIRDEVNGC IRLVYDMYST FGFEKIVVKL 
0421 STRPEKRIGS DEMWDRAEAD LAVALEENNI PFEYQLGEGA FYGPKIEFTL YDCLDRAWQC GTVQLDFSLP 
0491 SRLSASYVGE DNERKVPVMI HRAILGSMER FIGILTEEFA GFFPTWLAPV QVVIMNITDS QSEYVNELTQ 
0561 KLSNAGIRVK ADLRNEKIGF KIREHTLRRV PYMLVCGDKE VESGKVAVRT RRGKDLGSMD VNEVIEKLQQ 
0631 EIRSRSLKQL EE

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             catttgttgg ctagatggtt tcgcaatgaa 
-070 ctgttaataa acaaattttt ctttgtatgt gatctttcgt gtgggtcacc actgcaaata aggatataaa 
0001 atgcctgtta taactcttcc tgatggcagc caacgccatt acgatcacgc tgtaagcccc atggatgttg 
0071 cgctggacat tggtccaggt ctggcgaaag cctgtatcgc agggcgcgtt aatggcgaac tggttgatgc 
0141 ttgcgatctg attgaaaacg acgcacaact gtcgatcatt accgccaaag acgaagaagg tctggagatc 
0211 attcgtcact cctgtgcgca cctgttaggg cacgcgatta aacaactttg gccgcatacc aaaatggcaa 
0281 tcggcccggt tattgacaac ggtttttatt acgacgttga tcttgaccgc acgttaaccc aggaagatgt 
0351 cgaagcactc gagaagcgga tgcatgagct tgctgagaaa aactacgacg tcattaagaa gaaagtcagc 
0421 tggcacgaag cgcgtgaaac tttcgccaac cgtggggaga gctacaaagt ctccattctt gacgaaaaca 
0491 tcgcccatga tgacaagcca ggtctgtact tccatgaaga atatgtcgat atgtgccgcg gtccgcacgt 
0561 accgaacatg cgtttctgcc atcatttcaa actaatgaaa acggcagggg cttactggcg tggcgacagc 
0631 aacaacaaaa tgttgcaacg tatttacggt acggcgtggg cagacaaaaa agcacttaac gcttacctgc 
0701 agcgcctgga agaagccgcg aaacgcgacc accgtaaaat cggtaaacag ctcgacctgt accatatgca 
0771 ggaagaagcg ccgggtatgg tattctggca caacgacggc tggaccatct tccgtgaact ggaagtgttt 
0841 gttcgttcta aactgaaaga gtaccagtat caggaagtta aaggtccgtt catgatggac cgtgtcctgt 
0911 gggaaaaaac cggtcactgg gacaactaca aagatgcaat gttcaccaca tcttctgaga accgtgaata 
0981 ctgcattaag ccgatgaact gcccgggtca cgtacaaatt ttcaaccagg ggctgaagtc ttatcgcgat 
1051 ctgccgctgc gtatggccga gtttggtagc tgccaccgta acgagccgtc aggttcgctg catggcctga 
1121 tgcgcgtgcg tggatttacc caggatgacg cgcatatctt ctgtactgaa gaacaaattc gcgatgaagt 
1191 taacggatgt atccgtttag tctatgatat gtacagcact tttggcttcg agaagatcgt cgtcaaactc 
1261 tccactcgtc ctgaaaaacg tattggcagc gacgaaatgt gggatcgtgc tgaggcggac ctggcggttg 
1331 cgctggaaga aaacaacatc ccgtttgaat atcaactggg tgaaggcgct ttctacggtc cgaaaattga 
1401 atttaccctg tatgactgcc tcgatcgtgc atggcagtgc ggtacagtac agctggactt ctctttgccg 
1471 tctcgtctga gcgcttctta tgtaggcgaa gacaatgaac gtaaagtacc ggtaatgatt caccgcgcaa 
1541 ttctggggtc gatggaacgt ttcatcggta tcctgaccga agagttcgct ggtttcttcc cgacctggct 
1611 tgcgccggtt caggttgtta tcatgaatat taccgattca cagtctgaat acgttaacga attgacgcaa 
1681 aaactatcaa atgcgggcat tcgtgttaaa gcagacttga gaaatgagaa gattggcttt aaaatccgcg 
1751 agcacacttt gcgtcgcgtc ccatatatgc tggtctgtgg tgataaagag gtggaatcag gcaaagttgc 
1821 cgttcgcacc cgccgtggta aagacctggg aagcatggac gtaaatgaag tgatcgagaa gctgcaacaa 
1891 gagattcgca gccgcagtct taaacaattg gaggaataag gtattaaagg cggaaaacga gttcaaacgg 
1961 cgcgccctaa ccgtatcaat ggcgaaattc gcgcccagga agttcgctta acaggtctgg aaggcgagc