MG1655
W3110
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Gene Report : pheS
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
J Bacteriol. 1976;127(2):923-33.
Genes for the alpha and beta subunits of the phenylalanyl-transfer ribonucleic acid synthetase of Escherichia coli.
Comer MM, Bock A. ( 783122 )
     Deletion OCL85,86-8 (AK4-4 / BK6-4) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  pheS
Related strains
     Strains Search Search strains by gene name:  pheS   Search strains by all related name:  pheS b1714 ECK1712 JW5277 phe-act

General information  (Go to Linear View:)
 Gene Name pheS  
 Alternative name b1714,ECK1712,JW5277,phe-act  
 Location, Length 1,795,983 - 1,796,966 (  -  ) ;   38.71 min ; 984 (bp) ,   327 (aa) Go to Linear View
 Product phenylalanine tRNA synthetase, alpha subunit  
 Operon Name thrS-infC-rpmI-rplT-pheMST-ihfA  
 Note phenylalanine tRNA synthetase, alpha-subunit; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006418 - tRNA aminoacylation for protein translation  
 Function enzyme; Aminoacyl tRNA synthetases, tRNA modification  
 Gene Ontology GO:0000166; nucleotide binding ( pheS )
GO:0000287; magnesium ion binding ( pheS )
GO:0004812; aminoacyl-tRNA ligase activity ( pheS )
GO:0004826; phenylalanine-tRNA ligase activity ( pheS )
GO:0005515; protein binding ( pheS )
GO:0005524; ATP binding ( pheS )
GO:0005737; cytoplasm ( pheS )
GO:0006412; translation ( pheS )
GO:0006418; tRNA aminoacylation for protein translation ( pheS )
GO:0006432; phenylalanyl-tRNA aminoacylation ( pheS )
GO:0016874; ligase activity ( pheS )
GO:0046872; metal ion binding ( pheS )
 PID 1788007  
 EC number
  (KEGG Pathway)
 
6.1.1.20  
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) SYFA_ECOLI ( P08312 ; P78233; Q59406 )
    -  Protein name Phenylalanyl-tRNA synthetase alpha chain  
    -  Synonyms EC 6.1.1.20, Phenylalanine--tRNA ligase alpha chain, PheRS  
    -  Gene name Name=pheS; OrderedLocusNames=b1714;  

 Linear View (Whole Mode)
View Location
   1785.0  –  1810.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP pheS (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Phenylalanyl-Trna Synthetase From Thermus Thermophilus  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Phenylalanyl-tRNA synthetase alpha chain (Phenylalanine--tRNA ligase alpha chain) (PheRS)  
         PSI-BLAST Phenylalanyl-tRNA synthetase alpha chain
    nr     (database updated : 2007.02.20 )
         BLAST phenylalanyl-tRNA synthetase alpha subunit [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AMIDATION    AA_TRNA_LIGASE_II_1    CRYSTALLIN_BETAGAMMA    AA_TRNA_LIGASE_II_2     

Other Cross-Reference
    COG COG0016J 
    EcoCyc pheS 

MMBR References
J Bacteriol. 1982;152(1):357-62.
Molecular cloning and regulation of expression of the genes for initiation factor 3 and two aminoacyl-tRNA synthetases.
Elseviers D, Gallagher P, Hoffman A, Weinberg B, Schwartz I. ( 6749810 )
J Biol Chem. 1964;239:1839-1843
Demonstration of an altered aminoacyl ribonucleic acid synthetase in a mutant of Escherichia coli.
Fangman, W. L., and F. C. Neidhardt.
J Mol Biol. 1983;171(3):239-61.
Escherichia coli phenylalanyl-tRNA synthetase operon region. Evidence for an attenuation mechanism. Identification of the gene for the ribosomal protein L20.
Fayat G, Mayaux JF, Sacerdot C, Fromant M, Springer M, Grunberg-Manago M, Blanquet S. ( 6317865 )
J Bacteriol. 1968;95(3):991-7.
Selection of temperature-sensitive activating enzyme mutants in Escherichia coli.
Kaplan S, Anderson D. ( 4868365 )
J Bacteriol. 1985;163(2):787-91.
Sequence of the Escherichia coli pheST operon and identification of the himA gene.
Mechulam Y, Fayat G, Blanquet S. ( 2991205 )
J Mol Biol. 1987;197(3):453-70.
Dual level control of the Escherichia coli pheST-himA operon expression. tRNA(Phe)-dependent attenuation and transcriptional operator-repressor control by himA and the SOS network.
Mechulam Y, Blanquet S, Fayat G. ( 3326947 )
J Mol Biol. 1980;144(4):595-600.
Genes for the two subunits of phenylalanyl-tRNA synthesis of Escherichia coli are transcribed from the same promoter.
Plumbridge JA, Springer M. ( 6166752 )
J Bacteriol. 1982;152(2):661-8.
Escherichia coli phenylalanyl-tRNA synthetase operon: transcription studies of wild-type and mutated operons on multicopy plasmids.
Plumbridge JA, Springer M. ( 6215395 )
Gene. 1980;11(1-2):33-42.
Physical localisation and cloning of the structural gene for E. coli initiation factor IF3 from a group of genes concerned with translation.
Plumbridge JA, Springer M, Graffe M, Goursot R, Grunberg-Manago M. ( 6254853 )
Mol Gen Genet. 1982;186(2):247-52.
Transcription units around the gene for E. coli translation initiation factor IF3 (infC).
Springer M, Plumbridge JA, Trudel M, Graffe M, Grunberg-Manago M. ( 7050631 )
J Mol Biol. 1985;181(4):467-78.
Attenuation control of the Escherichia coli phenylalanyl-tRNA synthetase operon.
Springer M, Mayaux JF, Fayat G, Plumbridge JA, Graffe M, Blanquet S, Grunberg-Manago M. ( 3158742 )
J Mol Biol. 1983;171(3):263-79.
Escherichia coli phenylalanyl-tRNA synthetase operon is controlled by attenuation in vivo.
Springer M, Trudel M, Graffe M, Plumbridge J, Fayat G, Mayaux JF, Sacerdot C, Blanquet S, Grunberg-Manago M. ( 6317866 )
J Mol Biol. 1984;173(2):177-209.
Transcription of a gene cluster coding for two aminoacyl-tRNA synthetases and an initiation factor in Escherichia coli.
Wu TH, Wood DL, Stein PL, Comer MM. ( 6368838 )

Sequences
Amino acid
FASTA format
0001 MSHLAELVAS AKAAISQASD VAALDNVRVE YLGKKGHLTL QMTTLRELPP EERPAAGAVI NEAKEQVQQA 
0071 LNARKAELES AALNARLAAE TIDVSLPGRR IENGGLHPVT RTIDRIESFF GELGFTVATG PEIEDDYHNF 
0141 DALNIPGHHP ARADHDTFWF DTTRLLRTQT SGVQIRTMKA QQPPIRIIAP GRVYRNDYDQ THTPMFHQME 
0211 GLIVDTNISF TNLKGTLHDF LRNFFEEDLQ IRFRPSYFPF TEPSAEVDVM GKNGKWLEVL GCGMVHPNVL 
0281 RNVGIDPEVY SGFAFGMGME RLTMLRYGVT DLRSFFENDL RFLKQFK

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             tcgagcacag cccgggacca aaatggcaag 
-070 taaaatagcc tgatgggata ggctctaagt ccaacgaacc agtgtcacca ctgacacaat gaggaaaacc 
0001 atgtcacatc tcgcagaact ggttgccagt gcgaaggcgg ccattagcca ggcgtcagat gttgccgcgt 
0071 tagataatgt gcgcgtcgaa tatttgggta aaaaagggca cttaaccctt cagatgacga ccctgcgtga 
0141 gctgccgcca gaagagcgtc cggcagctgg tgcggttatc aacgaagcga aagagcaggt tcagcaggcg 
0211 ctgaatgcgc gtaaagcgga actggaaagc gctgcactga atgcgcgtct ggcggcggaa acgattgatg 
0281 tctctctgcc aggtcgtcgc attgaaaacg gcggtctgca tccggttacc cgtaccatcg accgtatcga 
0351 aagtttcttc ggtgagcttg gctttaccgt ggcaaccggg ccggaaatcg aagacgatta tcataacttc 
0421 gatgctctga acattcctgg tcaccacccg gcgcgcgctg accacgacac tttctggttt gacactaccc 
0491 gcctgctgcg tacccagacc tctggcgtac agatccgcac catgaaagcc cagcagccac cgattcgtat 
0561 catcgcgcct ggccgtgttt atcgtaacga ctacgaccag actcacacgc cgatgttcca tcagatggaa 
0631 ggtctgattg ttgataccaa catcagcttt accaacctga aaggcacgct gcacgacttc ctgcgtaact 
0701 tctttgagga agatttgcag attcgcttcc gtccttccta cttcccgttt accgaacctt ctgcagaagt 
0771 ggacgtcatg ggtaaaaacg gtaaatggct ggaagtgctg ggctgcggga tggtgcatcc gaacgtgttg 
0841 cgtaacgttg gcatcgaccc ggaagtttac tctggtttcg ccttcgggat ggggatggag cgtctgacta 
0911 tgttgcgtta cggcgtcacc gacctgcgtt cattcttcga aaacgatctg cgtttcctca aacagtttaa 
0981 ataaggcagg aatagattat gaaattcagt gaactgtggt tacgcgaatg ggtgaacccg gcgattgata 
1051 gcgatgcgct ggcaaatcaa atcactatgg cggg