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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Bacteriol. 1976;127(2):923-33.
Genes for the alpha and beta subunits of the phenylalanyl-transfer ribonucleic acid synthetase of Escherichia coli.
Comer MM, Bock A.
(
783122
)
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Deletion
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OCL85,86-8 (AK4-4 / BK6-4)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
pheT
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Related strains
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Strains Search
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Search strains by gene name:
pheT
Search strains by all related name:
pheT b1713 ECK1711 JW1703
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General information (Go to Linear View: )
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Gene Name
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pheT
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Alternative name
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b1713,ECK1711,JW1703
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Location, Length
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1,793,581 - 1,795,968
(
-
)
;
38.66
min
;
2388
(bp) ,
795
(aa)
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Product
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phenylalanine tRNA synthetase, beta subunit
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Operon Name
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thrS-infC-rpmI-rplT-pheMST-ihfA
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Note
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phenylalanine tRNA synthetase, beta-subunit; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006418 - tRNA aminoacylation for protein translation
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Function
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enzyme; Aminoacyl tRNA synthetases, tRNA modification
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Gene Ontology
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GO:0000049
;
tRNA binding ( pheT )
GO:0000166
;
nucleotide binding ( pheT )
GO:0000287
;
magnesium ion binding ( pheT )
GO:0003723
;
RNA binding ( pheT )
GO:0004812
;
aminoacyl-tRNA ligase activity ( pheT )
GO:0004826
;
phenylalanine-tRNA ligase activity ( pheT )
GO:0005515
;
protein binding ( pheT )
GO:0005524
;
ATP binding ( pheT )
GO:0005624
;
membrane fraction ( pheT )
GO:0005737
;
cytoplasm ( pheT )
GO:0006412
;
translation ( pheT )
GO:0006432
;
phenylalanyl-tRNA aminoacylation ( pheT )
GO:0008033
;
tRNA processing ( pheT )
GO:0016874
;
ligase activity ( pheT )
GO:0046872
;
metal ion binding ( pheT )
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PID
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1788006
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EC number
(KEGG Pathway)
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6.1.1.20
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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SYFB_ECOLI
(
P07395
;
Q59407
)
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- Protein name
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Phenylalanyl-tRNA synthetase beta chain
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- Synonyms
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EC 6.1.1.20, Phenylalanine-- tRNA ligase beta chain, PheRS
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- Gene name
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Name=pheT; OrderedLocusNames=b1713;
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MMBR References
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J Bacteriol. 1982;152(1):357-62.
Molecular cloning and regulation of expression of the genes for initiation factor 3 and two aminoacyl-tRNA synthetases.
Elseviers D, Gallagher P, Hoffman A, Weinberg B, Schwartz I.
(
6749810
)
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J Mol Biol. 1983;171(3):239-61.
Escherichia coli phenylalanyl-tRNA synthetase operon region. Evidence for an attenuation mechanism. Identification of the gene for the ribosomal protein L20.
Fayat G, Mayaux JF, Sacerdot C, Fromant M, Springer M, Grunberg-Manago M, Blanquet S.
(
6317865
)
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J Bacteriol. 1985;163(2):787-91.
Sequence of the Escherichia coli pheST operon and identification of the himA gene.
Mechulam Y, Fayat G, Blanquet S.
(
2991205
)
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J Mol Biol. 1987;197(3):453-70.
Dual level control of the Escherichia coli pheST-himA operon expression. tRNA(Phe)-dependent attenuation and transcriptional operator-repressor control by himA and the SOS network.
Mechulam Y, Blanquet S, Fayat G.
(
3326947
)
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J Mol Biol. 1980;144(4):595-600.
Genes for the two subunits of phenylalanyl-tRNA synthesis of Escherichia coli are transcribed from the same promoter.
Plumbridge JA, Springer M.
(
6166752
)
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J Bacteriol. 1982;152(2):661-8.
Escherichia coli phenylalanyl-tRNA synthetase operon: transcription studies of wild-type and mutated operons on multicopy plasmids.
Plumbridge JA, Springer M.
(
6215395
)
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Gene. 1980;11(1-2):33-42.
Physical localisation and cloning of the structural gene for E. coli initiation factor IF3 from a group of genes concerned with translation.
Plumbridge JA, Springer M, Graffe M, Goursot R, Grunberg-Manago M.
(
6254853
)
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Mol Gen Genet. 1982;186(2):247-52.
Transcription units around the gene for E. coli translation initiation factor IF3 (infC).
Springer M, Plumbridge JA, Trudel M, Graffe M, Grunberg-Manago M.
(
7050631
)
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J Mol Biol. 1985;181(4):467-78.
Attenuation control of the Escherichia coli phenylalanyl-tRNA synthetase operon.
Springer M, Mayaux JF, Fayat G, Plumbridge JA, Graffe M, Blanquet S, Grunberg-Manago M.
(
3158742
)
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J Mol Biol. 1984;173(2):177-209.
Transcription of a gene cluster coding for two aminoacyl-tRNA synthetases and an initiation factor in Escherichia coli.
Wu TH, Wood DL, Stein PL, Comer MM.
(
6368838
)
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| Sequences |
Amino acid
FASTA format
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0001 MKFSELWLRE WVNPAIDSDA LANQITMAGL EVDGVEPVAG SFHGVVVGEV VECAQHPNAD KLRVTKVNVG
0071 GDRLLDIVCG APNCRQGLRV AVATIGAVLP GDFKIKAAKL RGEPSEGMLC SFSELGISDD HSGIIELPAD
0141 APIGTDIREY LKLDDNTIEI SVTPNRADCL GIIGVARDVA VLNQLPLVQP EIVPVGATID DTLPITVEAP
0211 EACPRYLGRV VKGINVKAPT PLWMKEKLRR CGIRSIDAVV DVTNYVLLEL GQPMHAFDKD RIEGGIVVRM
0281 AKEGETLVLL DGTEAKLNAD TLVIADHNKA LAMGGIFGGE HSGVNDETQN VLLECAFFSP LSITGRARRH
0351 GLHTDASHRY ERGVDPALQH KAMERATRLL IDICGGEAGP VIDITNEATL PKRATITLRR SKLDRLIGHH
0421 IADEQVTDIL RRLGCEVTEG KDEWQAVAPS WRFDMEIEED LVEEVARVYG YNNIPDEPVQ ASLIMGTHRE
0491 ADLSLKRVKT LLNDKGYQEV ITYSFVDPKV QQMIHPGVEA LLLPSPISVE MSAMRLSLWT GLLATVVYNQ
0561 NRQQNRVRIF ESGLRFVPDT QAPLGIRQDL MLAGVICGNR YEEHWNLAKE TVDFYDLKGD LESVLDLTGK
0631 LNEVEFRAEA NPALHPGQSA AIYLKGERIG FVGVVHPELE RKLDLNGRTL VFELEWNKLA DRVVPQAREI
0701 SRFPANRRDI AVVVAENVPA ADILSECKKV GVNQVVGVNL FDVYRGKGVA EGYKSLAISL ILQDTSRTLE
0771 EEEIAATVAK CVEALKERFQ ASLRD
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 agcgtctgac tatgttgcgt tacggcgtca
-070 ccgacctgcg ttcattcttc gaaaacgatc tgcgtttcct caaacagttt aaataaggca ggaatagatt
0001 atgaaattca gtgaactgtg gttacgcgaa tgggtgaacc cggcgattga tagcgatgcg ctggcaaatc
0071 aaatcactat ggcgggcctg gaagttgacg gtgtagaacc ggttgccggc agcttccacg gcgtggtcgt
0141 tggtgaagtg gttgagtgtg cgcagcatcc gaacgctgac aaactgcgtg tgacaaaagt gaatgtcggc
0211 ggcgatcgcc tgctggacat cgtctgcggt gcgccaaact gccgtcaggg cctgcgtgta gcggtagcga
0281 ccattggtgc tgttctgccg ggtgatttca aaattaaagc ggcgaaactg cgtggcgaac cgtctgaagg
0351 gatgctgtgc tccttctctg aactgggcat ttctgacgat cacagcggca ttatcgaact gcctgcggat
0421 gcgccgattg gcaccgatat ccgtgaatac ctgaaacttg atgacaacac catcgaaatc agcgtgacgc
0491 caaaccgtgc cgactgctta ggcatcattg gtgttgcgcg tgacgttgcc gtgctgaacc agctgccgct
0561 ggttcaaccg gaaatcgttc cggttggtgc gaccatcgac gacacgctgc cgattacagt cgaagcgccg
0631 gaagcctgcc cgcgttatct tggccgtgtg gtaaaaggca ttaacgttaa agcgccaact ccgctgtgga
0701 tgaaagaaaa actgcgtcgt tgcgggatcc gttctatcga tgcagttgtt gacgtcacca actatgtgct
0771 gctcgaactg ggccagccga tgcacgcttt cgataaagat cgcattgaag gcggcattgt ggtgcggatg
0841 gcgaaagagg gcgaaacgct ggtgctgctc gacggtactg aagcgaagct gaatgctgac actctggtca
0911 tcgccgacca caacaaggcg ctggcgatgg gcggcatctt cggtggcgaa cactctggcg tgaatgacga
0981 aacacaaaac gtgctgctgg aatgcgcgtt ctttagcccg ctgtctatca ccggtcgtgc tcgtcgtcat
1051 ggcctgcata ccgatgcgtc tcaccgttat gagcgtggcg ttgatccggc actgcagcac aaagcgatgg
1121 aacgtgcgac ccgtctgctg atcgacatct gcggtggtga ggctggcccg gtaattgata tcaccaacga
1191 agcaacgctg ccgaagcgtg caaccatcac tctacgtcgt agcaaactgg atcgcctgat cggccatcat
1261 attgcggatg agcaggtaac tgacattctg cgtcgtctcg gctgcgaagt gaccgaaggc aaagacgaat
1331 ggcaggcagt tgcgccgagc tggcgtttcg atatggagat tgaagaagat ctggttgaag aagtcgcgcg
1401 tgtttacggc tacaacaaca tcccggatga gccggtacag gcaagcctga ttatgggtac tcaccgtgaa
1471 gctgacctgt cgctcaagcg cgtgaaaacg ctgctcaacg acaaaggcta tcaggaagtg atcacctaca
1541 gcttcgttga tccgaaagtg cagcagatga tccatccagg cgttgaagcc ttactgctgc caagcccgat
1611 ctctgttgaa atgtcagcaa tgcgtctttc tctgtggact ggcctgctgg caaccgtggt gtacaaccag
1681 aaccgtcagc agaaccgtgt gcgcattttc gaaagcggtc tgcgtttcgt accagatact caggcaccgt
1751 tgggcattcg tcaggatctg atgttagccg gtgtgatttg cggtaaccgt tacgaagagc actggaacct
1821 ggcaaaagag accgttgatt tctatgattt gaaaggcgat cttgaatccg ttctcgacct gaccggtaaa
1891 ctgaatgagg ttgagttccg tgcagaagcg aatccggcac tgcatccggg gcaatccgca gcgatttatc
1961 tgaaaggtga acgtattggt tttgttgggg ttgttcatcc tgaactggaa cgtaaactgg atcttaacgg
2031 tcgcactctg gtgttcgaac tggagtggaa caagctcgca gaccgcgtgg tgcctcaggc gcgcgagatt
2101 tctcgcttcc cggcgaaccg tcgtgacatc gcggtggtgg tcgcagaaaa cgttcccgca gcggatattt
2171 tatccgaatg taagaaagtt ggcgtaaatc aggtagttgg cgtaaactta tttgacgtgt accgcggtaa
2241 gggtgttgcg gaggggtata agagcctcgc cataagcctg atcctgcaag ataccagccg tacactcgaa
2311 gaagaggaga ttgccgctac cgtcgccaaa tgtgtagagg cattaaaaga gcgattccag gcatcattga
2381 gggattgaac ctatggcgct tacaaaagct gaaatgtcag aatatctgtt tgataagctt gggcttagca
2451 agcgggatgc caaagaactg gttgaactgt ttttcgaa
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