MG1655
W3110
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Gene Report : pheT
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
J Bacteriol. 1976;127(2):923-33.
Genes for the alpha and beta subunits of the phenylalanyl-transfer ribonucleic acid synthetase of Escherichia coli.
Comer MM, Bock A. ( 783122 )
     Deletion OCL85,86-8 (AK4-4 / BK6-4) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  pheT
Related strains
     Strains Search Search strains by gene name:  pheT   Search strains by all related name:  pheT b1713 ECK1711 JW1703

General information  (Go to Linear View:)
 Gene Name pheT  
 Alternative name b1713,ECK1711,JW1703  
 Location, Length 1,793,581 - 1,795,968 (  -  ) ;   38.66 min ; 2388 (bp) ,   795 (aa) Go to Linear View
 Product phenylalanine tRNA synthetase, beta subunit  
 Operon Name thrS-infC-rpmI-rplT-pheMST-ihfA  
 Note phenylalanine tRNA synthetase, beta-subunit; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006418 - tRNA aminoacylation for protein translation  
 Function enzyme; Aminoacyl tRNA synthetases, tRNA modification  
 Gene Ontology GO:0000049; tRNA binding ( pheT )
GO:0000166; nucleotide binding ( pheT )
GO:0000287; magnesium ion binding ( pheT )
GO:0003723; RNA binding ( pheT )
GO:0004812; aminoacyl-tRNA ligase activity ( pheT )
GO:0004826; phenylalanine-tRNA ligase activity ( pheT )
GO:0005515; protein binding ( pheT )
GO:0005524; ATP binding ( pheT )
GO:0005624; membrane fraction ( pheT )
GO:0005737; cytoplasm ( pheT )
GO:0006412; translation ( pheT )
GO:0006432; phenylalanyl-tRNA aminoacylation ( pheT )
GO:0008033; tRNA processing ( pheT )
GO:0016874; ligase activity ( pheT )
GO:0046872; metal ion binding ( pheT )
 PID 1788006  
 EC number
  (KEGG Pathway)
 
6.1.1.20  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) SYFB_ECOLI ( P07395 ; Q59407 )
    -  Protein name Phenylalanyl-tRNA synthetase beta chain  
    -  Synonyms EC 6.1.1.20, Phenylalanine-- tRNA ligase beta chain, PheRS  
    -  Gene name Name=pheT; OrderedLocusNames=b1713;  

 Linear View (Whole Mode)
View Location
   1785.0  –  1810.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP pheT (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain B, Phenylalanyl-Trna Synthetase From Thermus Thermophilus Complexed With Trna And A Phenylalanyl-Adenylate Analog  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Phenylalanyl-tRNA synthetase beta chain  
         PSI-BLAST Phenylalanyl-tRNA synthetase beta chain
    nr     (database updated : 2007.02.20 )
         BLAST phenylalanyl-tRNA synthetase beta subunit [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    BPD_TRANSP_INN_MEMBR     

Other Cross-Reference
    COG COG0072J  COG0073R 
    EcoCyc pheT 

MMBR References
J Bacteriol. 1982;152(1):357-62.
Molecular cloning and regulation of expression of the genes for initiation factor 3 and two aminoacyl-tRNA synthetases.
Elseviers D, Gallagher P, Hoffman A, Weinberg B, Schwartz I. ( 6749810 )
J Mol Biol. 1983;171(3):239-61.
Escherichia coli phenylalanyl-tRNA synthetase operon region. Evidence for an attenuation mechanism. Identification of the gene for the ribosomal protein L20.
Fayat G, Mayaux JF, Sacerdot C, Fromant M, Springer M, Grunberg-Manago M, Blanquet S. ( 6317865 )
J Bacteriol. 1985;163(2):787-91.
Sequence of the Escherichia coli pheST operon and identification of the himA gene.
Mechulam Y, Fayat G, Blanquet S. ( 2991205 )
J Mol Biol. 1987;197(3):453-70.
Dual level control of the Escherichia coli pheST-himA operon expression. tRNA(Phe)-dependent attenuation and transcriptional operator-repressor control by himA and the SOS network.
Mechulam Y, Blanquet S, Fayat G. ( 3326947 )
J Mol Biol. 1980;144(4):595-600.
Genes for the two subunits of phenylalanyl-tRNA synthesis of Escherichia coli are transcribed from the same promoter.
Plumbridge JA, Springer M. ( 6166752 )
J Bacteriol. 1982;152(2):661-8.
Escherichia coli phenylalanyl-tRNA synthetase operon: transcription studies of wild-type and mutated operons on multicopy plasmids.
Plumbridge JA, Springer M. ( 6215395 )
Gene. 1980;11(1-2):33-42.
Physical localisation and cloning of the structural gene for E. coli initiation factor IF3 from a group of genes concerned with translation.
Plumbridge JA, Springer M, Graffe M, Goursot R, Grunberg-Manago M. ( 6254853 )
Mol Gen Genet. 1982;186(2):247-52.
Transcription units around the gene for E. coli translation initiation factor IF3 (infC).
Springer M, Plumbridge JA, Trudel M, Graffe M, Grunberg-Manago M. ( 7050631 )
J Mol Biol. 1985;181(4):467-78.
Attenuation control of the Escherichia coli phenylalanyl-tRNA synthetase operon.
Springer M, Mayaux JF, Fayat G, Plumbridge JA, Graffe M, Blanquet S, Grunberg-Manago M. ( 3158742 )
J Mol Biol. 1984;173(2):177-209.
Transcription of a gene cluster coding for two aminoacyl-tRNA synthetases and an initiation factor in Escherichia coli.
Wu TH, Wood DL, Stein PL, Comer MM. ( 6368838 )

Sequences
Amino acid
FASTA format
0001 MKFSELWLRE WVNPAIDSDA LANQITMAGL EVDGVEPVAG SFHGVVVGEV VECAQHPNAD KLRVTKVNVG 
0071 GDRLLDIVCG APNCRQGLRV AVATIGAVLP GDFKIKAAKL RGEPSEGMLC SFSELGISDD HSGIIELPAD 
0141 APIGTDIREY LKLDDNTIEI SVTPNRADCL GIIGVARDVA VLNQLPLVQP EIVPVGATID DTLPITVEAP 
0211 EACPRYLGRV VKGINVKAPT PLWMKEKLRR CGIRSIDAVV DVTNYVLLEL GQPMHAFDKD RIEGGIVVRM 
0281 AKEGETLVLL DGTEAKLNAD TLVIADHNKA LAMGGIFGGE HSGVNDETQN VLLECAFFSP LSITGRARRH 
0351 GLHTDASHRY ERGVDPALQH KAMERATRLL IDICGGEAGP VIDITNEATL PKRATITLRR SKLDRLIGHH 
0421 IADEQVTDIL RRLGCEVTEG KDEWQAVAPS WRFDMEIEED LVEEVARVYG YNNIPDEPVQ ASLIMGTHRE 
0491 ADLSLKRVKT LLNDKGYQEV ITYSFVDPKV QQMIHPGVEA LLLPSPISVE MSAMRLSLWT GLLATVVYNQ 
0561 NRQQNRVRIF ESGLRFVPDT QAPLGIRQDL MLAGVICGNR YEEHWNLAKE TVDFYDLKGD LESVLDLTGK 
0631 LNEVEFRAEA NPALHPGQSA AIYLKGERIG FVGVVHPELE RKLDLNGRTL VFELEWNKLA DRVVPQAREI 
0701 SRFPANRRDI AVVVAENVPA ADILSECKKV GVNQVVGVNL FDVYRGKGVA EGYKSLAISL ILQDTSRTLE 
0771 EEEIAATVAK CVEALKERFQ ASLRD

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             agcgtctgac tatgttgcgt tacggcgtca 
-070 ccgacctgcg ttcattcttc gaaaacgatc tgcgtttcct caaacagttt aaataaggca ggaatagatt 
0001 atgaaattca gtgaactgtg gttacgcgaa tgggtgaacc cggcgattga tagcgatgcg ctggcaaatc 
0071 aaatcactat ggcgggcctg gaagttgacg gtgtagaacc ggttgccggc agcttccacg gcgtggtcgt 
0141 tggtgaagtg gttgagtgtg cgcagcatcc gaacgctgac aaactgcgtg tgacaaaagt gaatgtcggc 
0211 ggcgatcgcc tgctggacat cgtctgcggt gcgccaaact gccgtcaggg cctgcgtgta gcggtagcga 
0281 ccattggtgc tgttctgccg ggtgatttca aaattaaagc ggcgaaactg cgtggcgaac cgtctgaagg 
0351 gatgctgtgc tccttctctg aactgggcat ttctgacgat cacagcggca ttatcgaact gcctgcggat 
0421 gcgccgattg gcaccgatat ccgtgaatac ctgaaacttg atgacaacac catcgaaatc agcgtgacgc 
0491 caaaccgtgc cgactgctta ggcatcattg gtgttgcgcg tgacgttgcc gtgctgaacc agctgccgct 
0561 ggttcaaccg gaaatcgttc cggttggtgc gaccatcgac gacacgctgc cgattacagt cgaagcgccg 
0631 gaagcctgcc cgcgttatct tggccgtgtg gtaaaaggca ttaacgttaa agcgccaact ccgctgtgga 
0701 tgaaagaaaa actgcgtcgt tgcgggatcc gttctatcga tgcagttgtt gacgtcacca actatgtgct 
0771 gctcgaactg ggccagccga tgcacgcttt cgataaagat cgcattgaag gcggcattgt ggtgcggatg 
0841 gcgaaagagg gcgaaacgct ggtgctgctc gacggtactg aagcgaagct gaatgctgac actctggtca 
0911 tcgccgacca caacaaggcg ctggcgatgg gcggcatctt cggtggcgaa cactctggcg tgaatgacga 
0981 aacacaaaac gtgctgctgg aatgcgcgtt ctttagcccg ctgtctatca ccggtcgtgc tcgtcgtcat 
1051 ggcctgcata ccgatgcgtc tcaccgttat gagcgtggcg ttgatccggc actgcagcac aaagcgatgg 
1121 aacgtgcgac ccgtctgctg atcgacatct gcggtggtga ggctggcccg gtaattgata tcaccaacga 
1191 agcaacgctg ccgaagcgtg caaccatcac tctacgtcgt agcaaactgg atcgcctgat cggccatcat 
1261 attgcggatg agcaggtaac tgacattctg cgtcgtctcg gctgcgaagt gaccgaaggc aaagacgaat 
1331 ggcaggcagt tgcgccgagc tggcgtttcg atatggagat tgaagaagat ctggttgaag aagtcgcgcg 
1401 tgtttacggc tacaacaaca tcccggatga gccggtacag gcaagcctga ttatgggtac tcaccgtgaa 
1471 gctgacctgt cgctcaagcg cgtgaaaacg ctgctcaacg acaaaggcta tcaggaagtg atcacctaca 
1541 gcttcgttga tccgaaagtg cagcagatga tccatccagg cgttgaagcc ttactgctgc caagcccgat 
1611 ctctgttgaa atgtcagcaa tgcgtctttc tctgtggact ggcctgctgg caaccgtggt gtacaaccag 
1681 aaccgtcagc agaaccgtgt gcgcattttc gaaagcggtc tgcgtttcgt accagatact caggcaccgt 
1751 tgggcattcg tcaggatctg atgttagccg gtgtgatttg cggtaaccgt tacgaagagc actggaacct 
1821 ggcaaaagag accgttgatt tctatgattt gaaaggcgat cttgaatccg ttctcgacct gaccggtaaa 
1891 ctgaatgagg ttgagttccg tgcagaagcg aatccggcac tgcatccggg gcaatccgca gcgatttatc 
1961 tgaaaggtga acgtattggt tttgttgggg ttgttcatcc tgaactggaa cgtaaactgg atcttaacgg 
2031 tcgcactctg gtgttcgaac tggagtggaa caagctcgca gaccgcgtgg tgcctcaggc gcgcgagatt 
2101 tctcgcttcc cggcgaaccg tcgtgacatc gcggtggtgg tcgcagaaaa cgttcccgca gcggatattt 
2171 tatccgaatg taagaaagtt ggcgtaaatc aggtagttgg cgtaaactta tttgacgtgt accgcggtaa 
2241 gggtgttgcg gaggggtata agagcctcgc cataagcctg atcctgcaag ataccagccg tacactcgaa 
2311 gaagaggaga ttgccgctac cgtcgccaaa tgtgtagagg cattaaaaga gcgattccag gcatcattga 
2381 gggattgaac ctatggcgct tacaaaagct gaaatgtcag aatatctgtt tgataagctt gggcttagca 
2451 agcgggatgc caaagaactg gttgaactgt ttttcgaa