|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
|
Deletion
|
OCL86
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
ppsA
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
ppsA
Search strains by all related name:
ppsA b1702 ECK1700 f792 JW1692 pps ppsA
|
General information (Go to Linear View: )
|
|
Gene Name
|
ppsA
|
|
Alternative name
|
b1702,ECK1700,f792,JW1692,pps,ppsA
|
|
Location, Length
|
1,782,758 - 1,785,136
(
-
)
;
38.42
min
;
2379
(bp) ,
792
(aa)
|
|
Product
|
phosphoenolpyruvate synthase
|
|
Operon Name
|
ppsA
|
|
Note
|
GO_process: GO:0006094 - gluconeogenesis
|
|
Function
|
enzyme; Central intermediary metabolism: Gluconeogenesis
|
|
Gene Ontology
|
GO:0000166
;
nucleotide binding ( ppsA )
GO:0000287
;
magnesium ion binding ( ppsA )
GO:0003824
;
catalytic activity ( ppsA )
GO:0005515
;
protein binding ( ppsA )
GO:0005524
;
ATP binding ( ppsA )
GO:0006094
;
gluconeogenesis ( ppsA )
GO:0008986
;
pyruvate, water dikinase activity ( ppsA )
GO:0016301
;
kinase activity ( ppsA )
GO:0016310
;
phosphorylation ( ppsA )
GO:0016740
;
transferase activity ( ppsA )
GO:0016772
;
transferase activity, transferring phosphorus-containing groups ( ppsA )
GO:0046872
;
metal ion binding ( ppsA )
|
|
PID
|
1787994
|
EC number
(KEGG Pathway)
|
2.7.9.2
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
PPSA_ECOLI
(
P23538
)
|
|
- Protein name
|
Phosphoenolpyruvate synthase
|
|
- Synonyms
|
EC 2.7.9.2, Pyruvate, water dikinase, PEP synthase
|
|
- Gene name
|
Name=ppsA; Synonyms=pps; OrderedLocusNames=b1702;
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Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
pps
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain , Pyruvate Phosphate Dikinase
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Phosphoenolpyruvate synthase
|
|
PSI-BLAST
|
Phosphoenolpyruvate synthase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
phosphoenolpyruvate synthase [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
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Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
GLYCOSAMINOGLYCAN
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
PEP_ENZYMES_PHOS_SITE
PEP_ENZYMES_2
|
|
MMBR References
|
Mol Gen Genet. 1992;231(2):332-6.
Cloning and nucleotide sequence of the Escherichia coli K-12 ppsA gene, encoding PEP synthase.
Niersbach M, Kreuzaler F, Geerse RH, Postma PW, Hirsch HJ.
(
1310524
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSNNGSSPLV LWYNQLGMND VDRVGGKNAS LGEMITNLSG MGVSVPNGFA TTADAFNQFL DQSGVNQRIY
0071 ELLDKTDIDD VTQLAKAGAQ IRQWIIDTPF QPELENAIRE AYAQLSADDE NASFAVRSSA TAEDMPDASF
0141 AGQQETFLNV QGFDAVLVAV KHVFASLFND RAISYRVHQG YDHRGVALSA GVQRMVRSDL ASSGVMFSID
0211 TESGFDQVVF ITSAWGLGEM VVQGAVNPDE FYVHKPTLAA NRPAIVRRTM GSKKIRMVYA PTQEHGKQVK
0281 IEDVPQEQRD IFSLTNEEVQ ELAKQAVQIE KHYGRPMDIE WAKDGHTGKL FIVQARPETV RSRGQVMERY
0351 TLHSQGKIIA EGRAIGHRIG AGPVKVIHDI SEMNRIEPGD VLVTDMTDPD WEPIMKKASA IVTNRGGRTC
0421 HAAIIARELG IPAVVGCGDA TERMKDGENV TVSCAEGDTG YVYAELLEFS VKSSSVETMP DLPLKVMMNV
0491 GNPDRAFDFA CLPNEGVGLA RLEFIINRMI GVHPRALLEF DDQEPQLQNE IREMMKGFDS PREFYVGRLT
0561 EGIATLGAAF YPKRVIVRLS DFKSNEYANL VGGERYEPDE ENPMLGFRGA GRYVSDSFRD CFALECEAVK
0631 RVRNDMGLTN VEIMIPFVRT VDQAKAVVEE LARQGLKRGE NGLKIIMMCE IPSNALLAEQ FLEYFDGFSI
0701 GSNDMTQLAL GLDRDSGVVS ELFDERNDAV KALLSMAIRA AKKQGKYVGI CGQGPSDHED FAAWLMEEGI
0771 DSLSLNPDTV VQTWLSLAEL KK
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ttgaacgatt caccgttttt ttcatccggt
-070 taaatatgca aagataaatg cgcagaaatg tgtttctcaa accgttcatt tatcacaaaa ggattgttcg
0001 atgtccaaca atggctcgtc accgctggtg ctttggtata accaactcgg catgaatgat gtagacaggg
0071 ttgggggcaa aaatgcctcc ctgggtgaaa tgattactaa tctttccgga atgggtgttt ccgttccgaa
0141 tggtttcgcc acaaccgccg acgcgtttaa ccagtttctg gaccaaagcg gcgtaaacca gcgcatttat
0211 gaactgctgg ataaaacgga tattgacgat gttactcagc ttgcgaaagc gggcgcgcaa atccgccagt
0281 ggattatcga cactcccttc cagcctgagc tggaaaacgc catccgcgaa gcctatgcac agctttccgc
0351 cgatgacgaa aacgcctctt ttgcggtgcg ctcctccgcc accgcagaag atatgccgga cgcttctttt
0421 gccggtcagc aggaaacctt cctcaacgtt cagggttttg acgccgttct cgtggcagtg aaacatgtat
0491 ttgcttctct gtttaacgat cgcgccatct cttatcgtgt gcaccagggt tacgatcacc gtggtgtggc
0561 gctctccgcc ggtgttcaac ggatggtgcg ctctgacctc gcatcatctg gcgtgatgtt ctccattgat
0631 accgaatccg gctttgacca ggtggtgttt atcacttccg catggggcct tggtgagatg gtcgtgcagg
0701 gtgcggttaa cccggatgag ttttacgtgc ataaaccgac actggcggcg aatcgcccgg ctatcgtgcg
0771 ccgcaccatg gggtcgaaaa aaatccgcat ggtttacgcg ccgacccagg agcacggcaa gcaggttaaa
0841 atcgaagacg taccgcagga acagcgtgac atcttctcgc tgaccaacga agaagtgcag gaactggcaa
0911 aacaggccgt acaaattgag aaacactacg gtcgcccgat ggatattgag tgggcgaaag atggccacac
0981 cggtaaactg ttcattgtgc aggcgcgtcc ggaaaccgtg cgctcacgcg gtcaggtcat ggagcgttat
1051 acgctgcatt cacagggtaa gattatcgcc gaaggccgtg ctatcggtca tcgcatcggt gcgggtccgg
1121 tgaaagtcat ccatgacatc agcgaaatga accgcatcga acctggcgac gtgctggtta ctgacatgac
1191 cgacccggac tgggaaccga tcatgaagaa agcatctgcc atcgtcacca accgtggcgg tcgtacctgt
1261 cacgcggcga tcatcgctcg tgaactgggc attccggcgg tagtgggctg tggagatgca acagaacgga
1331 tgaaagacgg tgagaacgtc actgtttctt gtgccgaagg tgataccggt tacgtctatg cggagttgct
1401 ggaatttagc gtgaaaagct ccagcgtaga aacgatgccg gatctgccgt tgaaagtgat gatgaacgtc
1471 ggtaacccgg accgtgcttt cgacttcgcc tgcctaccga acgaaggcgt gggccttgcg cgtctggaat
1541 ttatcatcaa ccgtatgatt ggcgtccacc cacgcgcact gcttgagttt gacgatcagg aaccgcagtt
1611 gcaaaacgaa atccgcgaga tgatgaaagg ttttgattct ccgcgtgaat tttacgttgg tcgtctgact
1681 gaagggatcg cgacgctggg tgccgcgttt tatccgaagc gcgtcattgt ccgtctctct gattttaaat
1751 cgaacgaata tgccaacctg gtcggtggtg agcgttacga gccagatgaa gagaacccga tgctcggctt
1821 ccgtggcgcg ggccgctatg tttccgacag cttccgcgac tgtttcgcgc tggagtgtga agcagtgaaa
1891 cgtgtgcgca acgacatggg actgaccaac gttgagatca tgatcccgtt cgtgcgtacc gtagatcagg
1961 cgaaagcggt ggttgaagaa ctggcgcgtc aggggctgaa acgtggcgag aacgggctga aaatcatcat
2031 gatgtgtgaa atcccgtcca acgccttgct ggccgagcag ttcctcgaat atttcgacgg cttctcaatt
2101 ggctcaaacg atatgacgca gctggcgctc ggtctggacc gtgactccgg cgtggtgtct gaattgttcg
2171 atgagcgcaa cgatgcggtg aaagcactgc tgtcgatggc tatccgtgcc gcgaagaaac agggcaaata
2241 tgtcgggatt tgcggtcagg gtccgtccga ccacgaagac tttgccgcat ggttgatgga agaggggatc
2311 gatagcctgt ctctgaaccc ggacaccgtg gtgcaaacct ggttaagcct ggctgaactg aagaaataaa
2381 ataaatcccc ggcggcgttt agtcgccggg gttatgtgat ccccgaagat gaaacttatt caatctcttc
2451 acagacatcc tgcgttaaac gccgcataa
|
