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PEC Original Annotations
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Essentiality
|
|
Class
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non-essential
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|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
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Deletion
|
OCL88
(D)
,
OCL88-1
(D)
,
OCL88-8
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
cfa
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Related strains
|
|
Strains Search
|
Search strains by gene name:
cfa
Search strains by all related name:
cfa b1661 cdfA ECK1657 JW1653
|
General information (Go to Linear View: )
|
|
Gene Name
|
cfa
|
|
Alternative name
|
b1661,cdfA,ECK1657,JW1653
|
|
Location, Length
|
1,739,437 - 1,740,585
(
+
)
;
37.49
min
;
1149
(bp) ,
382
(aa)
|
|
Product
|
cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase)
|
|
Operon Name
|
cfa
|
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Note
|
cyclopropane fatty acyl phospholipid synthase
|
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Function
|
enzyme; Fatty acid and phosphatidic acid biosynthesis
|
|
Gene Ontology
|
GO:0005515
;
protein binding ( cfa )
GO:0005737
;
cytoplasm ( cfa )
GO:0008168
;
methyltransferase activity ( cfa )
GO:0008610
;
lipid biosynthetic process ( cfa )
GO:0008825
;
cyclopropane-fatty-acyl-phospholipid synthase activity ( cfa )
GO:0016740
;
transferase activity ( cfa )
|
|
PID
|
1787951
|
EC number
(KEGG Pathway)
|
2.1.1.79
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
cfa
(
EG11531
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show details
[
1
more]
)
|
Entry name(Acc.no)
|
CFA_ECOLI
(
P30010
)
|
|
- Protein name
|
Cyclopropane-fatty-acyl-phospholipid synthase
|
|
- Synonyms
|
EC 2.1.1.79, Cyclopropane fatty acid synthase, CFA synthase
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- Gene name
|
Name=cfa; Synonyms=cdfA; OrderedLocusNames=b1661, c2055;
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MMBR References
|
Gene. 1983;22(1):75-83.
Use of lambda phasmids for deletion mapping of non-selectable markers cloned in plasmids.
Grogan DW, Cronan JE Jr.
(
6305773
)
|
J Bacteriol. 1984;158(1):286-95.
Cloning and manipulation of the Escherichia coli cyclopropane fatty acid synthase gene: physiological aspects of enzyme overproduction.
Grogan DW, Cronan JE Jr.
(
6325391
)
|
Mol Gen Genet. 1984;196(2):367-72.
Genetic characterization of the Escherichia coli cyclopropane fatty acid (cfa) locus and neighboring loci.
Grogan DW, Cronan JE Jr.
(
6092874
)
|
Biochemistry. 1992;31(45):11020-8.
Cyclopropane fatty acid synthase of Escherichia coli: deduced amino acid sequence, purification, and studies of the enzyme active site.
Wang AY, Grogan DW, Cronan JE Jr.
(
1445840
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSSSCIEEVS VPDDNWYRIA NELLSRAGIA INGSAPADIR VKNPDFFKRV LQEGSLGLGE SYMDGWWECD
0071 RLDMFFSKVL RAGLENQLPH HFKDTLRIAG ARLFNLQSKK RAWIVGKEHY DLGNDLFSRM LDPFMQYSCA
0141 YWKDADNLES AQQAKLKMIC EKLQLKPGMR VLDIGCGWGG LAHYMASNYD VSVVGVTISA EQQKMAQERC
0211 EGLDVTILLQ DYRDLNDQFD RIVSVGMFEH VGPKNYDTYF AVVDRNLKPE GIFLLHTIGS KKTDLNVDPW
0281 INKYIFPNGC LPSVRQIAQS SEPHFVMEDW HNFGADYDTT LMAWYERFLA AWPEIADNYS ERFKRMFTYY
0351 LNACAGAFRA RDIQLWQVVF SRGVENGLRV AR
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GATTATAGAC TCGCGGTTTT TTCTGCGAGA
-070 TTTCTCACAA AGCCCAAAAA GCGTCTACGC TGTTTTAAGG TTCTGATCAC CGACCAGTGA TGGAGAAACT
0001 atgagttcat cgtgtataga agaagtcagt gtaccggatg acaactggta ccgtatcgcc aacgaattac
0071 ttagccgtgc cggtatagcc attaacggtt ctgccccggc ggatattcgt gtgaaaaacc ccgatttttt
0141 taaacgcgtt ctgcaagaag gctctttggg gttaggcgaa agttatatgg atggctggtg ggaatgtgac
0211 cgactggata tgttttttag caaagtctta cgcgcaggtc tcgagaacca actcccccat catttcaaag
0281 acacgctgcg tattgccggc gctcgtctct tcaatctgca gagtaaaaaa cgtgcctgga tagtcggcaa
0351 agagcattac gatttgggta atgacttgtt cagccgcatg cttgatccct tcatgcaata ttcctgcgct
0421 tactggaaag atgccgataa tctggaatct gcccagcagg cgaagctcaa aatgatttgt gaaaaattgc
0491 agttaaaacc agggatgcgc gtactggata ttggctgcgg ctggggcgga ctggcacact acatggcatc
0561 taattatgac gtaagcgtgg tgggcgtcac catttctgcc gaacagcaaa aaatggctca ggaacgctgt
0631 gaaggcctgg atgtcaccat tttgctgcaa gattatcgtg acctgaacga ccagtttgat cgtattgttt
0701 ctgtggggat gttcgagcac gtcggaccga aaaattacga tacctatttt gcggtggtgg atcgtaattt
0771 gaaaccggaa ggcatattcc tgctccatac tatcggttcg aaaaaaaccg atctgaatgt tgatccctgg
0841 attaataaat atatttttcc gaacggttgc ctgccctctg tacgccagat tgctcagtcc agcgaacccc
0911 actttgtgat ggaagactgg cataacttcg gtgctgatta cgatactacg ttgatggcgt ggtatgaacg
0981 attcctcgcc gcatggccag aaattgcgga taactatagt gaacgcttta aacgaatgtt tacctattat
1051 ctgaatgcct gtgcaggtgc tttccgcgcc cgtgatattc agctctggca ggtcgtgttc tcacgcggtg
1121 ttgaaaacgg ccttcgagtg gctcgctaaA GGCTATTCTA TCGCCCCCTC TCCGGGGGCG ATTTCAGATC
1191 AGGCTTCTGT GCCTGGTTGA TTCATGGCAT TTTCTCGTGC CGCCAGCACA CGTTCTACC
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