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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Bacteriol. 1969;100(1):167-75.
Mutants of Escherichia coli with an altered tyrosyl-transfer ribonucleic acid synthetase.
Schlesinger S, Nester EW.
(
4898984
)
|
|
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Deletion
|
OCL89,90-1
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
tyrS
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Related strains
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Strains Search
|
Search strains by gene name:
tyrS
Search strains by all related name:
tyrS b1637 ECK1633 JW1629
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General information (Go to Linear View: )
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Gene Name
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tyrS
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Alternative name
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b1637,ECK1633,JW1629
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Location, Length
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1,713,972 - 1,715,246
(
-
)
;
36.94
min
;
1275
(bp) ,
424
(aa)
|
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Product
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tyrosyl-tRNA synthetase
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Operon Name
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pdxH-tyrS-pdxY
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Note
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tyrosine tRNA synthetase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006418 - tRNA aminoacylation for protein translation
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Function
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enzyme; Aminoacyl tRNA synthetases, tRNA modification
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Gene Ontology
|
GO:0000166
;
nucleotide binding ( tyrS )
GO:0003723
;
RNA binding ( tyrS )
GO:0004812
;
aminoacyl-tRNA ligase activity ( tyrS )
GO:0004831
;
tyrosine-tRNA ligase activity ( tyrS )
GO:0005524
;
ATP binding ( tyrS )
GO:0005624
;
membrane fraction ( tyrS )
GO:0005737
;
cytoplasm ( tyrS )
GO:0006412
;
translation ( tyrS )
GO:0006418
;
tRNA aminoacylation for protein translation ( tyrS )
GO:0006437
;
tyrosyl-tRNA aminoacylation ( tyrS )
GO:0016874
;
ligase activity ( tyrS )
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PID
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1787925
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EC number
(KEGG Pathway)
|
6.1.1.1
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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tyrS
(
EG11043
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
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SYY_ECOLI
(
P00951
)
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- Protein name
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Tyrosyl-tRNA synthetase
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- Synonyms
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EC 6.1.1.1, Tyrosine--tRNA ligase, TyrRS
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- Gene name
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Name=tyrS; OrderedLocusNames=b1637, z2650, ECs2346;
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MMBR References
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Mol Gen Genet. 1980;178(3):647-50.
Improved mapping of the tyrS locus in Escherichia coli.
Diderichsen B, De Hauwer G.
(
6993861
)
|
FEBS Lett. 1982;150(2):419-23.
The tyrosyl-tRNA synthetase from Escherichia coli. Complete nucleotide sequence of the structural gene.
Barker DG, Bruton CJ, Winter G.
(
6761148
)
|
J Bacteriol. 1992;174:6033-6045
Characterization of the complex pdxH-tyrS operon of Escherichia coli K-12 and pleiotropic phenotypes caused by pdxH insertion mutations.
Lam, H. M., and M. E. Winkler.
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J Bacteriol. 1978;135(2):603-11.
Peptidase-deficient mutants of Escherichia coli.
Miller CG, Schwartz G.
(
355237
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MASSNLIKQL QERGLVAQVT DEEALAERLA QGPIALYCGF DPTADSLHLG HLVPLLCLKR FQQAGHKPVA
0071 LVGGATGLIG DPSFKAAERK LNTEETVQEW VDKIRKQVAP FLDFDCGENS AIAANNYDWF GNMNVLTFLR
0141 DIGKHFSVNQ MINKEAVKQR LNREDQGISF TEFSYNLLQG YDFACLNKQY GVVLQIGGSD QWGNITSGID
0211 LTRRLHQNQV FGLTVPLITK ADGTKFGKTE GGAVWLDPKK TSPYKFYQFW INTADADVYR FLKFFTFMSI
0281 EEINALEEED KNSGKAPRAQ YVLAEQVTRL VHGEEGLQAA KRITECLFSG SLSALSEADF EQLAQDGVPM
0351 VEMEKGADLM QALVDSELQP SRGQARKTIA SNAITINGEK QSDPEYFFKE EDRLFGRFTL LRRGKKNYCL
0421 ICWK
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 tggtactcct gattctggca ctttattcta
-070 tgtctctttc gcatctggcg aaaagtcgtg taccggcaaa ggtgcagtcg ttatatacat ggagattttg
0001 atggcaagca gtaacttgat taaacaattg caagagcggg ggctggtagc ccaggtgacg gacgaggaag
0071 cgttagcaga gcgactggcg caaggcccga tcgcgctcta ttgcggcttc gatcctaccg ctgacagctt
0141 gcatttgggg catcttgttc cattgttatg cctgaaacgc ttccagcagg cgggccacaa gccggttgcg
0211 ctggtaggcg gcgcgacggg tctgattggc gacccgagct tcaaagctgc cgagcgtaag ctgaacaccg
0281 aagaaactgt tcaggagtgg gtggacaaaa tccgtaagca ggttgccccg ttcctcgatt tcgactgtgg
0351 agaaaactct gctatcgcgg cgaacaacta tgactggttc ggcaatatga atgtgctgac cttcctgcgc
0421 gatattggca aacacttctc cgttaaccag atgatcaaca aagaagcggt taagcagcgt ctcaaccgtg
0491 aagatcaggg gatttcgttc actgagtttt cctacaacct gttgcagggt tatgacttcg cctgtctgaa
0561 caaacagtac ggtgtggtgc tgcaaattgg tggttctgac cagtggggta acatcacttc tggtatcgac
0631 ctgacccgtc gtctgcatca gaatcaggtg tttggcctga ccgttccgct gatcactaaa gcagatggca
0701 ccaaatttgg taaaactgaa ggcggcgcag tctggttgga tccgaagaaa accagcccgt acaaattcta
0771 ccagttctgg atcaacactg cggatgccga cgtttaccgc ttcctgaagt tcttcacctt tatgagcatt
0841 gaagagatca acgccctgga agaagaagat aaaaacagcg gtaaagcacc gcgcgcccag tatgtactgg
0911 cggagcaggt gactcgtctg gttcacggtg aagaaggttt acaggcggca aaacgtatta ccgaatgcct
0981 gttcagcggt tctttgagtg cgctgagtga agcggacttc gaacagctgg cgcaggacgg cgtaccgatg
1051 gttgagatgg aaaagggcgc agacctgatg caggcactgg tcgattctga actgcaacct tcccgtggtc
1121 aggcacgtaa aactatcgcc tccaatgcca tcaccattaa cggtgaaaaa cagtccgatc ctgaatactt
1191 ctttaaagaa gaagatcgtc tgtttggtcg ttttacctta ctgcgtcgcg gtaaaaagaa ttactgtctg
1261 atttgctgga aataatgcat taagtggaaa gggggagtga gaaatcactc cccctggttt ttatacaggg
1331 aacatgatga aaaatattct cgctatccag tctcacgttg tttat
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