|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
|
|
Deletion
|
DE(PLK1608)
,
OCL90-3
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
nth
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
nth
Search strains by all related name:
nth b1633 ECK1629 JW1625
|
General information (Go to Linear View: )
|
|
Gene Name
|
nth
|
|
Alternative name
|
b1633,ECK1629,JW1625
|
|
Location, Length
|
1,709,547 - 1,710,182
(
+
)
;
36.85
min
;
636
(bp) ,
211
(aa)
|
|
Product
|
DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
|
|
Operon Name
|
ydgK-rsxABCDGE-nth
|
|
Note
|
endonuclease III; specific for apurinic and/or apyrimidinic sites; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006308 - DNA catabolic process; GO_process: GO:0009314 - response to radiation
|
|
Function
|
enzyme; Degradation of DNA
|
|
Gene Ontology
|
GO:0003677
;
DNA binding ( nth )
GO:0003824
;
catalytic activity ( nth )
GO:0003906
;
DNA-(apurinic or apyrimidinic site) lyase activity ( nth )
GO:0004519
;
endonuclease activity ( nth )
GO:0005506
;
iron ion binding ( nth )
GO:0005515
;
protein binding ( nth )
GO:0005622
;
intracellular ( nth )
GO:0006281
;
DNA repair ( nth )
GO:0006284
;
base-excision repair ( nth )
GO:0006974
;
response to DNA damage stimulus ( nth )
GO:0008152
;
metabolic process ( nth )
GO:0016787
;
hydrolase activity ( nth )
GO:0016798
;
hydrolase activity, acting on glycosyl bonds ( nth )
GO:0016829
;
lyase activity ( nth )
GO:0043565
;
sequence-specific DNA binding ( nth )
GO:0046872
;
metal ion binding ( nth )
GO:0051536
;
iron-sulfur cluster binding ( nth )
GO:0051539
;
4 iron, 4 sulfur cluster binding ( nth )
|
|
PID
|
1787920
|
EC number
(KEGG Pathway)
|
4.2.99.18
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
nth
(
EG10662
)
|
|
Number of removed
|
0 aa cleaved
|
|
SWISS-PROT
(
Show details
[
1
more]
)
|
Entry name(Acc.no)
|
END3_ECOLI
(
P20625
)
|
|
- Protein name
|
Endonuclease III
|
|
- Synonyms
|
EC 4.2.99.18, DNA-(apurinic or apyrimidinic site) lyase
|
|
- Gene name
|
Name=nth; OrderedLocusNames=b1633, c2025;
|
|
MMBR References
|
Nucleic Acids Res. 1998;26:932-941
Multiply damaged sites in DNA: interactions with Escherichia coli endonucleases III and VIII.
Harrison, L., Z. Hatahet, A. A. Purmal, and S. S. Wallace.
|
Biochem J. 1987;242(2):565-72.
Escherichia coli endonuclease III is not an endonuclease but a beta-elimination catalyst.
Bailly V, Verly WG.
(
2439070
)
|
Pathol Biol (Paris). 1996;44(1):14-24.
Role of DNA repair enzymes in the cellular resistance to oxidative stress.
Laval J.
(
8734295
)
|
Mol Carcinog. 1996;16(4):188-96.
DNA base damage induced by ionizing radiation recognized by Escherichia coli UvrABC nuclease but not Nth or Fpg proteins.
Roldan-Arjona T, Sedgwick B.
(
8784461
)
|
J Bacteriol. 1997;179:3783-3785
Characterization of endonuclease III (nth) and endonuclease VIII (nei) mutants of Escherichia coli K-12.
Saito, Y., F. Uraki, S. Nakajima, A. Asaeda, K. Ono, K. Kubo, and K. Yamamoto.
|
J Bacteriol. 1985;162(2):607-10.
Genetic mapping of nth, a gene affecting endonuclease III (thymine glycol-DNA glycosylase) in Escherichia coli K-12.
Weiss B, Cunningham RP.
(
3886628
)
|
Proc Natl Acad Sci U S A. 1985;82:474-478
Endonuclease III (nth) mutants of Escherichia coli.
Cunningham, R. P., and B. Weiss.
|
| Sequences |
Amino acid
FASTA format
|
0001 MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE
0071 GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH
0141 IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD
0211 I
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 TGCTGGCAGG AAAATACCTG ATTGATGAAA
-070 GAATGAAAAA GCGCCGTGCT GAAGCAGCTG CAGAACGTGC ATTGCCAAAC GGTGAAACAG GGAATGTCTG
0001 atgaataaag caaaacgcct ggagatcctc actcgcctgc gtgagaacaa tcctcatccc accaccgagc
0071 ttaatttcag ttcgcctttt gaattgctga ttgccgtact gctttccgct caggcgaccg atgtcagtgt
0141 taataaggcg acggcgaaac tctacccggt ggcgaatacg cctgcagcga tgcttgaact gggcgttgaa
0211 ggggtgaaaa cctatatcaa aacgattggg ctttataaca gcaaagcaga aaatatcatc aaaacctgcc
0281 gtatcttgct ggagcagcat aatggcgagg ttccggaaga tcgtgctgcg cttgaagccc tgcccggcgt
0351 aggtcgtaaa acagccaacg tcgtattaaa cactgcattc ggctggccga ctattgctgt cgacacgcac
0421 attttccgcg tttgtaatcg tactcaattt gcgccgggga aaaacgtcga acaggtagaa gaaaagctac
0491 tgaaagtggt tccagcagag tttaaagtcg actgccacca ttggttgatc ctgcacgggc gttatacctg
0561 cattgcccgc aagccccgct gtggctcttg tattattgaa gatctttgtg aatacaaaga gaaagttgac
0631 atctgaAGAA AAGGGGTAAC ACCGATTACC CCATTGATAA CCTTTCTTTA TCCTCTTTTA AAACATCTTT
0701 TTAACGTCAA TGATGCCATT GCTTAGCGTT ATCATC
|
