MG1655
W3110
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Gene Report : nth
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion DE(PLK1608)  ,  OCL90-3 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  nth
Related strains
     Strains Search Search strains by gene name:  nth   Search strains by all related name:  nth b1633 ECK1629 JW1625

General information  (Go to Linear View:)
 Gene Name nth  
 Alternative name b1633,ECK1629,JW1625  
 Location, Length 1,709,547 - 1,710,182 (  +  ) ;   36.85 min ; 636 (bp) ,   211 (aa) Go to Linear View
 Product DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)  
 Operon Name ydgK-rsxABCDGE-nth  
 Note endonuclease III; specific for apurinic and/or apyrimidinic sites; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006308 - DNA catabolic process; GO_process: GO:0009314 - response to radiation  
 Function enzyme; Degradation of DNA  
 Gene Ontology GO:0003677 ; DNA binding ( nth )
GO:0003824 ; catalytic activity ( nth )
GO:0003906 ; DNA-(apurinic or apyrimidinic site) lyase activity ( nth )
GO:0004519 ; endonuclease activity ( nth )
GO:0005506 ; iron ion binding ( nth )
GO:0005515 ; protein binding ( nth )
GO:0005622 ; intracellular ( nth )
GO:0006281 ; DNA repair ( nth )
GO:0006284 ; base-excision repair ( nth )
GO:0006974 ; response to DNA damage stimulus ( nth )
GO:0008152 ; metabolic process ( nth )
GO:0016787 ; hydrolase activity ( nth )
GO:0016798 ; hydrolase activity, acting on glycosyl bonds ( nth )
GO:0016829 ; lyase activity ( nth )
GO:0043565 ; sequence-specific DNA binding ( nth )
GO:0046872 ; metal ion binding ( nth )
GO:0051536 ; iron-sulfur cluster binding ( nth )
GO:0051539 ; 4 iron, 4 sulfur cluster binding ( nth )
 PID 1787920  
 EC number
  (KEGG Pathway)
 
4.2.99.18  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene nth ( EG10662 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) END3_ECOLI ( P20625 )
    -  Protein name Endonuclease III  
    -  Synonyms EC 4.2.99.18, DNA-(apurinic or apyrimidinic site) lyase  
    -  Gene name Name=nth; OrderedLocusNames=b1633, c2025;  

 Linear View (Whole Mode)
View Location
   1700.0  –  1725.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP nth (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain , Refinement Of The Native Structure Of Endonuclease Iii To A Resolution Of 1.85 Angstrom  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Endonuclease III (DNA-(apurinic or apyrimidinic site) lyase)  
         PSI-BLAST Endonuclease III
    nr     (database updated : 2007.02.20 )
         BLAST DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AMIDATION    ENDONUCLEASE_III_1    ENDONUCLEASE_III_2     

Other Cross-Reference
    COG COG0177L 
    EcoCyc nth 

MMBR References
Nucleic Acids Res. 1998;26:932-941
Multiply damaged sites in DNA: interactions with Escherichia coli endonucleases III and VIII.
Harrison, L., Z. Hatahet, A. A. Purmal, and S. S. Wallace.
Biochem J. 1987;242(2):565-72.
Escherichia coli endonuclease III is not an endonuclease but a beta-elimination catalyst.
Bailly V, Verly WG. ( 2439070 )
Pathol Biol (Paris). 1996;44(1):14-24.
Role of DNA repair enzymes in the cellular resistance to oxidative stress.
Laval J. ( 8734295 )
Mol Carcinog. 1996;16(4):188-96.
DNA base damage induced by ionizing radiation recognized by Escherichia coli UvrABC nuclease but not Nth or Fpg proteins.
Roldan-Arjona T, Sedgwick B. ( 8784461 )
J Bacteriol. 1997;179:3783-3785
Characterization of endonuclease III (nth) and endonuclease VIII (nei) mutants of Escherichia coli K-12.
Saito, Y., F. Uraki, S. Nakajima, A. Asaeda, K. Ono, K. Kubo, and K. Yamamoto.
J Bacteriol. 1985;162(2):607-10.
Genetic mapping of nth, a gene affecting endonuclease III (thymine glycol-DNA glycosylase) in Escherichia coli K-12.
Weiss B, Cunningham RP. ( 3886628 )
Proc Natl Acad Sci U S A. 1985;82:474-478
Endonuclease III (nth) mutants of Escherichia coli.
Cunningham, R. P., and B. Weiss.

Sequences
Amino acid
FASTA format
0001 MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE 
0071 GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH 
0141 IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD 
0211 I

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             TGCTGGCAGG AAAATACCTG ATTGATGAAA 
-070 GAATGAAAAA GCGCCGTGCT GAAGCAGCTG CAGAACGTGC ATTGCCAAAC GGTGAAACAG GGAATGTCTG 
0001 atgaataaag caaaacgcct ggagatcctc actcgcctgc gtgagaacaa tcctcatccc accaccgagc 
0071 ttaatttcag ttcgcctttt gaattgctga ttgccgtact gctttccgct caggcgaccg atgtcagtgt 
0141 taataaggcg acggcgaaac tctacccggt ggcgaatacg cctgcagcga tgcttgaact gggcgttgaa 
0211 ggggtgaaaa cctatatcaa aacgattggg ctttataaca gcaaagcaga aaatatcatc aaaacctgcc 
0281 gtatcttgct ggagcagcat aatggcgagg ttccggaaga tcgtgctgcg cttgaagccc tgcccggcgt 
0351 aggtcgtaaa acagccaacg tcgtattaaa cactgcattc ggctggccga ctattgctgt cgacacgcac 
0421 attttccgcg tttgtaatcg tactcaattt gcgccgggga aaaacgtcga acaggtagaa gaaaagctac 
0491 tgaaagtggt tccagcagag tttaaagtcg actgccacca ttggttgatc ctgcacgggc gttatacctg 
0561 cattgcccgc aagccccgct gtggctcttg tattattgaa gatctttgtg aatacaaaga gaaagttgac 
0631 atctgaAGAA AAGGGGTAAC ACCGATTACC CCATTGATAA CCTTTCTTTA TCCTCTTTTA AAACATCTTT 
0701 TTAACGTCAA TGATGCCATT GCTTAGCGTT ATCATC