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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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EMBO J. 1993;12(8):3007-16.
Conversion of the FhuA transport protein into a diffusion channel through the outer membrane of Escherichia coli.
Killmann H, Benz R, Braun V.
(
7688295
)
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Deletion
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OCR33-15
(D)
,
OCR33-3
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
fhuA
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Related strains
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Strains Search
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Search strains by gene name:
fhuA
Search strains by all related name:
fhuA b0150 ECK0149 JW0146 T1 tonA
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General information (Go to Linear View: )
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Gene Name
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fhuA
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Alternative name
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b0150,ECK0149,JW0146,T1,tonA
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Location, Length
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167,484 - 169,727
(
+
)
;
3.61
min
;
2244
(bp) ,
747
(aa)
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Product
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ferrichrome outer membrane transporter
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Operon Name
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fhuACDB
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Note
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outer membrane protein receptor for ferrichrome, colicin M, and phages T1, T5, and phi80; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0009279 - cell outer membrane
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Function
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membrane; Outer membrane constituents
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Gene Ontology
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GO:0004872
;
receptor activity ( fhuA )
GO:0005215
;
transporter activity ( fhuA )
GO:0005506
;
iron ion binding ( fhuA )
GO:0006810
;
transport ( fhuA )
GO:0006811
;
ion transport ( fhuA )
GO:0006826
;
iron ion transport ( fhuA )
GO:0009279
;
cell outer membrane ( fhuA )
GO:0009597
;
detection of virus ( fhuA )
GO:0015343
;
siderophore-iron transmembrane transporter activity ( fhuA )
GO:0015891
;
siderophore transport ( fhuA )
GO:0016020
;
membrane ( fhuA )
GO:0016021
;
integral to membrane ( fhuA )
GO:0019867
;
outer membrane ( fhuA )
GO:0046718
;
entry of virus into host cell ( fhuA )
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PID
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1786344
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EC number
(KEGG Pathway)
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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fhuA
(
EG10302
)
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Number of removed
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33 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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FHUA_ECOLI
(
P06971
;
P71280; P75665
)
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- Protein name
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Ferrichrome-iron receptor precursor
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- Synonyms
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Ferric hydroxamate uptake, Ferric hydroxamate receptor
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- Gene name
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Name=fhuA; Synonyms=tonA; OrderedLocusNames=b0150;
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MMBR References
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J Bacteriol. 1983;156(3):1301-14.
Cloning and expression of the fhu genes involved in iron(III)-hydroxamate uptake by Escherichia coli.
Fecker L, Braun V.
(
6315685
)
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J Bacteriol. 1980;143(1):256-64.
Genetic control of hydroxamate-mediated iron uptake in Escherichia coli.
Kadner RJ, Heller K, Coulton JW, Braun V.
(
6249788
)
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EMBO J. 1993;12:3007-3016
Conversion of the FhuA transport protein into a diffusion channel through the outer membrane of Escherichia coli.
Killmann, H., R. Benz, and V. Braun.
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J Bacteriol. 1992;174:3479-3486
An aspartate deletion mutation defines a binding site of the multifunctional FhuA outer membrane receptor of Escherichia coli K-12.
Killmann, H., and V. Braun.
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FEMS Microbiol Lett. 1994;119(1-2):71-6.
Energy-dependent receptor activities of Escherichia coli K-12: mutated TonB proteins alter FhuA receptor activities to phages T5, T1, phi 80 and to colicin M.
Killmann H, Braun V.
(
8039674
)
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J Bacteriol. 1976;127(2):1036-7.
Iron transport in Salmonella typhimurium LT-2: prevention, by ferrichrome, of adsorption of bacteriophages ES18 and ES18.h1 to a common cell envelope receptor.
Luckey M, Neilands JB.
(
783114
)
|
J Bacteriol. 1972;111(3):731-8.
Iron uptake in Salmonella typhimurium: utilization of exogenous siderochromes as iron carriers.
Luckey M, Pollack JR, Wayne R, Ames BN, Neilands JB.
(
4559824
)
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Biochemistry. 1996;35(45):14216-24.
Purification and structural and functional characterization of FhuA, a transporter of the Escherichia coli outer membrane.
Boulanger P, le Maire M, Bonhivers M, Dubois S, Desmadril M, Letellier L.
(
8916906
)
|
FEBS Lett. 1994;346(1):59-64.
Energy-coupled transport through the outer membrane of Escherichia coli small deletions in the gating loop convert the FhuA transport protein into a diffusion channel.
Braun V, Killmann H, Benz R.
(
7515827
)
|
Mol Gen Genet. 1977;155(2):227-9.
Identification of the sid outer membrane receptor protein in Salmonella typhimurium SL1027.
Braun V, Hantke K, Stauder W.
(
337132
)
|
Mol Gen Genet. 1983;192(1-2):131-9.
Plasmid and chromosomal mutants in the iron(III)-aerobactin transport system of Escherichia coli. Use of streptonigrin for selection.
Braun V, Gross R, Koster W, Zimmermann L.
(
6316109
)
|
J Bacteriol. 1986;165(1):181-92.
Protein fusions of beta-galactosidase to the ferrichrome-iron receptor of Escherichia coli K-12.
Coulton JW, Mason P, Cameron DR, Carmel G, Jean R, Rode HN.
(
3079747
)
|
J Bacteriol. 1983;156(3):1315-21.
Molecular cloning of the ferrichrome-iron receptor of Escherichia coli K-12.
Coulton JW, Mason P, DuBow MS.
(
6315686
)
|
| Sequences |
Amino acid
FASTA format
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0001 MARSKTAQPK HSLRKIAVVV ATAVSGMSVY AQAAVEPKED TITVTAAPAP QESAWGPAAT IAARQSATGT
0071 KTDTPIQKVP QSISVVTAEE MALHQPKSVK EALSYTPGVS VGTRGASNTY DHLIIRGFAA EGQSQNNYLN
0141 GLKLQGNFYN DAVIDPYMLE RAEIMRGPVS VLYGKSSPGG LLNMVSKRPT TEPLKEVQFK AGTDSLFQTG
0211 FDFSDSLDDD GVYSYRLTGL ARSANAQQKG SEEQRYAIAP AFTWRPDDKT NFTFLSYFQN EPETGYYGWL
0281 PKEGTVEPLP NGKRLPTDFN EGAKNNTYSR NEKMVGYSFD HEFNDTFTVR QNLRFAENKT SQNSVYGYGV
0351 CSDPANAYSK QCAALAPADK GHYLARKYVV DDEKLQNFSV DTQLQSKFAT GDIDHTLLTG VDFMRMRNDI
0421 NAWFGYDDSV PLLNLYNPVN TDFDFNAKDP ANSGPYRILN KQKQTGVYVQ DQAQWDKVLV TLGGRYDWAD
0491 QESLNRVAGT TDKRDDKQFT WRGGVNYLFD NGVTPYFSYS ESFEPSSQVG KDGNIFAPSK GKQYEVGVKY
0561 VPEDRPIVVT GAVYNLTKTN NLMADPEGSF FSVEGGEIRA RGVEIEAKAA LSASVNVVGS YTYTDAEYTT
0631 DTTYKGNTPA QVPKHMASLW ADYTFFDGPL SGLTLGTGGR YTGSSYGDPA NSFKVGSYTV VDALVRYDLA
0701 RVGMAGSNVA LHVNNLFDRE YVASCFNTYG CFWGAERQVV ATATFRF
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CGTACCGCTT GCGAACCCGC CAGCGTTTCG
-070 AATATTATCT TATCTTTATA ATAATCATTC TCGTTTACGT TATCATTCAC TTTACATCAG AGATATACCA
0001 atggcgcgtt ccaaaactgc tcagccaaaa cactcactgc gtaaaatcgc agttgtagta gccacagcgg
0071 ttagcggcat gtctgtttat gcacaggcag cggttgaacc gaaagaagac actatcaccg ttaccgctgc
0141 acctgcgccg caagaaagcg catgggggcc tgctgcaact attgcggcgc gacagtctgc taccggcact
0211 aaaaccgata cgccgattca aaaagtgcca cagtctattt ctgttgtgac cgccgaagag atggcgctgc
0281 atcagccgaa gtcggtaaaa gaagcgctta gctacacgcc gggtgtctct gttggtacgc gtggcgcatc
0351 caacacctat gaccacctga tcattcgcgg ctttgcggca gaaggccaaa gccagaataa ctatctgaat
0421 ggcctgaagt tgcagggcaa cttctataac gatgcggtca ttgacccgta tatgctggaa cgcgctgaaa
0491 ttatgcgtgg cccggtttcc gtgctttacg gtaaaagcag tcctggcggc ctgttgaata tggtcagcaa
0561 gcgtccgacc accgaaccgc tgaaagaagt tcagtttaaa gccggtactg acagcctgtt ccagactggt
0631 tttgacttta gcgattcgtt ggatgatgac ggtgtttact cttatcgcct gaccggtctt gcgcgttctg
0701 ccaatgccca gcagaaaggg tcagaagagc agcgttatgc tattgcaccg gcgttcacct ggcgtccgga
0771 tgataaaacc aattttacct tcctttctta cttccagaac gagccggaaa ccggttatta cggctggttg
0841 ccgaaagagg gaaccgttga gccgctgccg aacggtaagc gtctgccgac agactttaat gaaggggcga
0911 agaacaacac ctattctcgt aatgagaaga tggtcggcta cagcttcgat cacgaattta acgacacctt
0981 tactgtgcgt cagaacctgc gctttgctga aaacaaaacc tcgcaaaaca gcgtttatgg ttacggcgtc
1051 tgctccgatc cggcgaatgc ttacagcaaa cagtgtgcgg cattagcgcc agcggataaa ggccattatc
1121 tggcacgtaa atacgtcgtt gatgatgaga agctgcaaaa cttctccgtt gatacccagt tgcagagcaa
1191 gtttgccact ggcgatatcg accacaccct gctgaccggt gtcgacttta tgcgtatgcg taatgacatc
1261 aacgcctggt ttggttacga cgactctgtg ccactgctca atctgtacaa tccggtgaat accgatttcg
1331 acttcaatgc caaagatccg gcaaactccg gcccttaccg cattctgaat aaacagaaac aaacgggcgt
1401 ttatgttcag gatcaggcgc agtgggataa agtgctggtc accctaggcg gtcgttatga ctgggcagat
1471 caagaatctc ttaaccgcgt tgccgggacg accgataaac gtgatgacaa acagtttacc tggcgtggtg
1541 gtgttaacta cctgtttgat aatggtgtaa caccttactt cagctatagc gaatcgtttg aaccttcttc
1611 gcaagttggg aaggatggta atattttcgc accgtctaaa ggtaagcagt atgaagtcgg cgtgaaatat
1681 gtaccggaag atcgtccgat tgtagttact ggtgccgtgt ataatctcac taaaaccaac aacctgatgg
1751 cggaccctga gggttccttc ttctcggttg aaggtggcga gatccgcgca cgtggcgtag aaatcgaagc
1821 gaaagcggcg ctgtcggcga gtgttaacgt agtcggttct tatacttaca ccgatgcgga atacaccacc
1891 gatactacct ataaaggcaa tacgcctgca caggtgccaa aacacatggc ttcgttgtgg gctgactaca
1961 ccttctttga cggtccgctt tcaggtctga cgctgggcac cggtggtcgt tatactggct ccagttatgg
2031 tgatccggct aactccttta aagtgggaag ttatacggtc gtggatgcgt tagtacgtta tgatctggcg
2101 cgagtcggca tggctggctc caacgtggcg ctgcatgtta acaacctgtt cgatcgtgaa tacgtcgcca
2171 gctgctttaa cacttatggc tgcttctggg gcgcagaacg tcaggtcgtt gcaaccgcaa ccttccgttt
2241 ctaaTTTCTC TTTTGGGGCA CGGATTTCCG TGCCCATTTC ACAAGTTGGC TGTTATGCAG GAATACACGA
2311 ATCATTCCGA TACCACTTTT GCACTGCGTA ATAT
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