|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Acta Microbiol Pol. 1997;46(1):7-17.
Escherichia coli defects caused by null mutations in dnaK and dnaJ genes.
Paciorek J, Kardys K, Lobacz B, Wolska KI.
(
9271843
)
|
|
|
Deletion
|
OCR27
(D)
,
OCR27-6
(D)
,
OCR27-7
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
dnaJ
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
dnaJ
Search strains by all related name:
dnaJ b0015 ECK0015 faa groA groB groC groP grpC JW0014
|
General information (Go to Linear View: )
|
|
Gene Name
|
dnaJ
|
|
Alternative name
|
b0015,ECK0015,faa,groA,groB,groC,groP,grpC,JW0014
|
|
Location, Length
|
14,168 - 15,298
(
+
)
;
0.31
min
;
1131
(bp) ,
376
(aa)
|
|
Product
|
chaperone Hsp40, co-chaperone with DnaK
|
|
Operon Name
|
dnaK-tpke11-dnaJ
|
|
Note
|
chaperone with DnaK; heat shock protein; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006457 - protein folding
|
|
Function
|
factor; Chaperones
|
|
Gene Ontology
|
GO:0005524
;
ATP binding ( dnaJ )
GO:0005737
;
cytoplasm ( dnaJ )
GO:0006260
;
DNA replication ( dnaJ )
GO:0006457
;
protein folding ( dnaJ )
GO:0006950
;
response to stress ( dnaJ )
GO:0008270
;
zinc ion binding ( dnaJ )
GO:0009408
;
response to heat ( dnaJ )
GO:0031072
;
heat shock protein binding ( dnaJ )
GO:0046872
;
metal ion binding ( dnaJ )
GO:0051082
;
unfolded protein binding ( dnaJ )
|
|
PID
|
1786197
|
EC number
(KEGG Pathway)
|
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
dnaJ
(
EG10240
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show details
[
4
more]
)
|
Entry name(Acc.no)
|
DNAB_ECOLI
(
P03005
)
|
|
- Protein name
|
Replicative DNA helicase
|
|
- Synonyms
|
EC 3.6.1.-
|
|
- Gene name
|
OrderedLocusNames=b4052, SF4154, S3577;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
dnaJ
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, The Crystal Structure Of Hsp40 Ydj1
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Chaperone protein dnaJ
|
|
PSI-BLAST
|
Chaperone protein dnaJ
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
chaperone with DnaK; heat shock protein [Shigella flexneri 2a str. 2457T]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
CYTOCHROME_C
DNAJ_1
DNAJ_CXXCXGXG
|
|
MMBR References
|
Proc Natl Acad Sci U S A. 1971;68:2977-2981
Bacterial mutants in which the gene N function of bacteriophage lambda is blocked have an altered RNA polymerase.
Georgopoulos, C.
|
J Biol Chem. 1996;271:30798-30803
Role of the heat shock protein DnaJ in the lon-dependent degradation of naturally unstable proteins.
Jubete, Y., M. R. Maurizi, and S. Gottesman.
|
J Biol Chem. 1986;261:1782-1785
The nucleotide sequence of the Escherichia coli K12 and dnaJ+ gene: a gene that encodes a heat shock protein.
Bardwell, J. C., K. Tilly, E. A. Craig, J. King, M. Zylicz, and C. Georgopoulos.
|
J Biol Chem. 1986;261:1778-1781
Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product.
Ohki, M., F. Tamura, S. Nishimura, and H. Uchida.
|
Biochim Biophys Acta. 1989;1009(1):94-8.
A complete deletion mutant of the Escherichia coli dnaKdnaJ operon.
Ohki R, Morita R, Kawamata T, Uchida H, Ohki M.
(
2675977
)
|
J Biol Chem. 1992;267:13180-13184
Escherichia coli dnaJ deletion mutation results in loss of stability of a positive regulator, CRP.
Ohki, R., T. Kawamata, Y. Katoh, F. Hosoda, and M. Ohki.
|
Eur J Biochem. 1973;35(2):331-7.
Biochemical and immunological characterization of threonyl-tRNA synthetase of two borrelidin-resistant mutants of Escherichia coli K12.
Paetz W, Nass G.
(
4577856
)
|
J Mol Biol. 1977;113(1):1-25.
Initiation of the DNA replication of bacteriophage lambda in Escherichia coli K12.
Saito H, Uchida H.
(
328896
)
|
Genes Dev. 1990;4:2202-2209
DnaK, DnaJ, and GrpE heat shock proteins negatively regulate heat shock gene expression by controlling the synthesis and stability of sigma 32.
Straus, D., W. Walter, and C. A. Gross.
|
Mol Gen Genet. 1977;151(1):27-34.
A new host gene (groPC) necessary for lambda DNA replication.
Sunshine M, Feiss M, Stuart J, Yochem J.
(
325364
)
|
Mol Gen Genet. 1983;192(1-2):10-4.
Genetic characterization of a gene for prolipoprotein signal peptidase in Escherichia coli.
Yamagata H, Taguchi N, Daishima K, Mizushima S.
(
6227793
)
|
J Biol Chem. 1995;270:22669-22672
A novel function of Escherichia coli chaperone DnaJ. Protein-disulfide isomerase.
de Crouy-Chanel, A., M. Kohiyama, and G. Richarme.
|
| Sequences |
Amino acid
FASTA format
|
0001 MAKQDYYEIL GVSKTAEERE IRKAYKRLAM KYHPDRNQGD KEAEAKFKEI KEAYEVLTDS QKRAAYDQYG
0071 HAAFEQGGMG GGGFGGGADF SDIFGDVFGD IFGGGRGRQR AARGADLRYN MELTLEEAVR GVTKEIRIPT
0141 LEECDVCHGS GAKPGTQPQT CPTCHGSGQV QMRQGFFAVQ QTCPHCQGRG TLIKDPCNKC HGHGRVERSK
0211 TLSVKIPAGV DTGDRIRLAG EGEAGEHGAP AGDLYVQVQV KQHPIFEREG NNLYCEVPIN FAMAALGGEI
0281 EVPTLDGRVK LKVPGETQTG KLFRMRGKGV KSVRGGAQGD LLCRVVVETP VGLNERQKQL LQELQESFGG
0351 PTGEHNSPRS KSFFDGVKKF FDDLTR
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GACAAAAAAT AATCGCCCTA TAAACGGGTA
-070 ATTATACTGA CACGGGCGAA GGGGAATTTC CTCTCCGCCC GTGCATTCAT CTAGGGGCAA TTTAAAAAAG
0001 atggctaagc aagattatta cgagatttta ggcgtttcca aaacagcgga agagcgtgaa atcagaaagg
0071 cctacaaacg cctggccatg aaataccacc cggaccgtaa ccagggtgac aaagaggccg aggcgaaatt
0141 taaagagatc aaggaagctt atgaagttct gaccgactcg caaaaacgtg cggcatacga tcagtatggt
0211 catgctgcgt ttgagcaagg tggcatgggc ggcggcggtt ttggcggcgg cgcagacttc agcgatattt
0281 ttggtgacgt tttcggcgat atttttggcg gcggacgtgg tcgtcaacgt gcggcgcgcg gtgctgattt
0351 acgctataac atggagctca ccctcgaaga agctgtacgt ggcgtgacca aagagatccg cattccgact
0421 ctggaagagt gtgacgtttg ccacggtagc ggtgcaaaac caggtacaca gccgcagact tgtccgacct
0491 gtcatggttc tggtcaggtg cagatgcgcc agggattctt cgctgtacag cagacctgtc cacactgtca
0561 gggccgcggt acgctgatca aagatccgtg caacaaatgt catggtcatg gtcgtgttga gcgcagcaaa
0631 acgctgtccg ttaaaatccc ggcaggggtg gacactggag accgcatccg tcttgcgggc gaaggtgaag
0701 cgggcgagca tggcgcaccg gcaggcgatc tgtacgttca ggttcaggtt aaacagcacc cgattttcga
0771 gcgtgaaggc aacaacctgt attgcgaagt cccgatcaac ttcgctatgg cggcgctggg tggcgaaatc
0841 gaagtaccga cccttgatgg tcgcgtcaaa ctgaaagtgc ctggcgaaac ccagaccggt aagctattcc
0911 gtatgcgcgg taaaggcgtc aagtctgtcc gcggtggcgc acagggtgat ttgctgtgcc gcgttgtcgt
0981 cgaaacaccg gtaggcctga acgaaaggca gaaacagctg ctgcaagagc tgcaagaaag cttcggtggc
1051 ccaaccggcg agcacaacag cccgcgctca aagagcttct ttgatggtgt gaagaagttt tttgacgacc
1121 tgacccgcta aCCTCCCCAA AAGCCTGCCC GTGGGCAGGC CTGGGTAAAA ATAGGGTGCG TTGAAGATAT
1191 GCGAGCACCT GTAAAGTGGC GGGGATCACT CCCATAAGCG C
|
