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PEC Original Annotations
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|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
|
|
Deletion
|
20
(LD)
,
DE(PLK1608)
,
OCR70,L90-5 (LD 20-1_P/O)
(D)
,
OCR70,L90-6 (LD 20-1_P/O & 20-2TC)
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
narY
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Related strains
|
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Strains Search
|
Search strains by gene name:
narY
Search strains by all related name:
narY b1467 chlZ ECK1461 JW1462
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General information (Go to Linear View: )
|
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Gene Name
|
narY
|
|
Alternative name
|
b1467,chlZ,ECK1461,JW1462
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|
Location, Length
|
1,535,333 - 1,536,877
(
-
)
;
33.09
min
;
1545
(bp) ,
514
(aa)
|
|
Product
|
nitrate reductase 2 (NRZ), beta subunit
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Operon Name
|
narZYWV
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Note
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GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009061 - anaerobic respiration
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Function
|
enzyme; Energy metabolism, carbon: Anaerobic respiration
|
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Gene Ontology
|
GO:0005506
;
iron ion binding ( narY )
GO:0005886
;
plasma membrane ( narY )
GO:0006118
;
electron transport ( narY )
GO:0006810
;
transport ( narY )
GO:0008940
;
nitrate reductase activity ( narY )
GO:0009055
;
electron carrier activity ( narY )
GO:0009325
;
nitrate reductase complex ( narY )
GO:0016020
;
membrane ( narY )
GO:0016491
;
oxidoreductase activity ( narY )
GO:0042126
;
nitrate metabolic process ( narY )
GO:0042128
;
nitrate assimilation ( narY )
GO:0046872
;
metal ion binding ( narY )
GO:0051536
;
iron-sulfur cluster binding ( narY )
GO:0051538
;
3 iron, 4 sulfur cluster binding ( narY )
GO:0051539
;
4 iron, 4 sulfur cluster binding ( narY )
|
|
PID
|
1787740
|
EC number
(KEGG Pathway)
|
1.7.99.4
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
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EcoGene
|
narY
(
EG10647
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
NARY_ECOLI
(
P19318
;
P78267
)
|
|
- Protein name
|
Respiratory nitrate reductase 2 beta chain
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- Synonyms
|
EC 1.7.99.4
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- Gene name
|
Name=narY; OrderedLocusNames=b1467;
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Homology Analysis
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BLAST
|
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Bacteria
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GTOP
|
narY
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
|
Chain B, Crystal Structure Of Nitrate Reductase A, Narghi, From Escherichia Coli
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Respiratory nitrate reductase 2 beta chain
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PSI-BLAST
|
Tenascin-X precursor
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|
nr
(database updated :
2007.02.20
)
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BLAST
|
nitrate reductase 2 (NRZ), beta subunit [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
|
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PROSITE
|
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PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
AMIDATION
CYTOCHROME_C
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MMBR References
|
Mol Gen Genet. 1990;222(1):104-11.
Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon.
Blasco F, Iobbi C, Ratouchniak J, Bonnefoy V, Chippaux M.
(
2233673
)
|
Mol Microbiol. 1992;6(2):221-30.
Involvement of the narJ or narW gene product in the formation of active nitrate reductase in Escherichia coli.
Blasco F, Pommier J, Augier V, Chippaux M, Giordano G.
(
1545706
)
|
FEMS Microbiol Lett. 1997;147(1):147-9.
Organization of the nar genes at the chlZ locus.
Bonnefoy V, Ratouchniak J, Blasco F, Chippaux M.
(
9037773
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MKIRSQVGMV LNLDKCIGCH TCSVTCKNVW TGREGMEYAW FNNVETKPGI GYPKNWEDQE EWQGGWVRDV
0071 NGKIRPRLGN KMGVITKIFA NPVVPQIDDY YEPFTFDYEH LHSAPEGKHI PTARPRSLID GKRMDKVIWG
0141 PNWEELLGGE FEKRARDRNF EAMQKEMYGQ FENTFMMYLP RLCEHCLNPS CVATCPSGAI YKREEDGIVL
0211 IDQDKCRGWR LCISGCPYKK IYFNWKSGKS EKCIFCYPRI ESGQPTVCSE TCVGRIRYLG VLLYDADRIE
0281 EAASTEREVD LYERQCEVFL DPHDPSVIEE ALKQGIPQNV IDAAQRSPVY KMAMDWKLAL PLHPEYRTLP
0351 MVWYVPPLSP IQSYADAGGL PKSEGVLPAI ESLRIPVQYL ANMLSAGDTG PVLRALKRMM AMRHYMRSQT
0421 VEGVTDTRAI DEVGLSVAQV EEMYRYLAIA NYEDRFVIPT SHREMAGDAF AERNGCGFTF GDGCHGSDSK
0491 FNLFNSSRID AINITEVRDK AEGE
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ccgtcggatc gaaccgcgat gagttcatca
-070 tgatccgcaa gatgaagaac gttaactggc tggatgatga aggtcgcgat caggtacagg aggcgaaaaa
0001 atgaaaatcc gttcacaagt cggcatggtg cttaacctcg acaaatgtat cggctgccat acctgttcgg
0071 tgacctgtaa aaacgtctgg accgggcgcg aaggcatgga gtacgcatgg tttaacaacg tcgaaaccaa
0141 accgggcatt ggttatccga aaaactggga agatcaggaa gagtggcaag gcggctgggt gcgtgatgtg
0211 aatggcaaga tacgcccgcg tctgggcaac aagatgggcg taataaccaa aatcttcgcc aacccggtgg
0281 tgccgcagat tgatgattac tacgaacctt tcaccttcga ctacgaacat ttgcatagcg caccggaagg
0351 caaacatatt cctactgctc gcccgcgttc actgattgac ggcaaacgga tggacaaagt gatctggggg
0421 ccaaactggg aagaactgct gggcggtgag ttcgaaaaac gtgcccgcga ccgcaacttc gaggccatgc
0491 aaaaggagat gtacgggcag tttgaaaaca ccttcatgat gtacctgccg cgcctgtgcg aacactgcct
0561 caatcccagc tgcgtggcga cctgcccaag cggcgctatc tacaaacgcg aagaagacgg cattgtgctg
0631 attgatcagg ataaatgccg tggctggcgt ttgtgcataa gcggttgtcc gtacaaaaaa atctacttca
0701 actggaaaag cggcaagtca gaaaaatgca tcttctgtta cccacgaatt gagtccggtc aaccgaccgt
0771 gtgctcagaa acctgcgtgg gtcgcatccg gtatctgggc gtgctgcttt acgacgccga ccgcatcgag
0841 gaagcggcga gcaccgagcg cgaagttgac ctctatgaac gccagtgcga agtgttcctc gatccacacg
0911 atccctcagt gatcgaggaa gccctgaaac aaggtattcc acaaaatgtg attgacgctg cccagcgttc
0981 gccagtctac aaaatggcga tggactggaa actggcacta ccgttgcacc ctgaataccg caccctgccg
1051 atggtctggt acgttcctcc gctgtcaccg attcagtcct acgcagatgc gggcggtttg ccgaaaagcg
1121 aaggcgtgct gcccgccatc gaaagcctgc gtattccggt gcaatatctc gccaatatgt tgagtgccgg
1191 cgataccggt ccggtactgc gggcgctgaa acggatgatg gcgatgcgcc actatatgcg ttcacaaacc
1261 gtggaaggcg ttactgatac tcgtgccatc gacgaagtag gcctgagcgt cgcccaggtc gaagagatgt
1331 atcgctatct cgccattgcc aactatgaag atcgttttgt tatcccgacc agccatcggg aaatggcggg
1401 cgatgccttc gcagaacgca acggctgcgg ttttaccttt ggcgacggtt gccacggttc ggacagtaaa
1471 ttcaacctgt tcaacagtag ccgtatcgat gccatcaaca tcaccgaagt gcgcgacaaa gcggagggcg
1541 aataatgcag atcctcaaag tgatcggcct gttgatggag tatccggacg agctgttgtg ggaatgcaag
1611 gaggacgcgc tggcgttgat ccgccgcgac gcgcc
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