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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
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OCR69-5
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
pyrF
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Related strains
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Strains Search
|
Search strains by gene name:
pyrF
Search strains by all related name:
pyrF b1281 ECK1276 JW1273
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General information (Go to Linear View: )
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Gene Name
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pyrF
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Alternative name
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b1281,ECK1276,JW1273
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Location, Length
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1,339,945 - 1,340,682
(
+
)
;
28.88
min
;
738
(bp) ,
245
(aa)
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Product
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orotidine-5'-phosphate decarboxylase
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Operon Name
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yciSM-pyrF-yciH
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Note
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GO_process: GO:0006221 - pyrimidine nucleotide biosynthetic process; GO_process: GO:0015949 - nucleobase, nucleoside and nucleotide interconversion
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Function
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enzyme; Pyrimidine ribonucleotide biosynthesis
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Gene Ontology
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GO:0003824
;
catalytic activity ( pyrF )
GO:0004590
;
orotidine-5'-phosphate decarboxylase activity ( pyrF )
GO:0005515
;
protein binding ( pyrF )
GO:0006207
;
'de novo' pyrimidine base biosynthetic process ( pyrF )
GO:0006221
;
pyrimidine nucleotide biosynthetic process ( pyrF )
GO:0008152
;
metabolic process ( pyrF )
GO:0016829
;
lyase activity ( pyrF )
GO:0016831
;
carboxy-lyase activity ( pyrF )
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PID
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1787537
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EC number
(KEGG Pathway)
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4.1.1.23
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
|
PYRF_ECOLI
(
P08244
)
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- Protein name
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Orotidine 5'-phosphate decarboxylase
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- Synonyms
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EC 4.1.1.23, OMP decarboxylase, OMPDCase, OMPdecase
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- Gene name
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Name=pyrF; OrderedLocusNames=b1281;
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MMBR References
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Gene. 1983;25(1):39-48.
Cloning and physical analysis of the pyrF gene (coding for orotidine-5'-phosphate decarboxylase) from Escherichia coli K-12.
Donovan WP, Kushner SR.
(
6319231
)
|
Eur J Biochem. 1984;140:343-352
Studies on the structure and expression of Escherichia coli pyrC, pyrD and pyrF using the cloned genes.
Jensen, K. F., J. N. Larsen, L. Schack, and A. Sivertsen.
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Mol Gen Genet. 1984;197(2):345-6.
A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.
Boeke JD, LaCroute F, Fink GR.
(
6394957
)
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J Biol Chem. 1987;262:10239-10245
Nucleotide sequence and characterization of the pyrF operon of Escherichia coli K-12.
Turnbough, C. L., K. H. Kerr, W. R. Funderburg, J. P. Donahue, and F. E. Powell.
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| Sequences |
Amino acid
FASTA format
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0001 MTLTASSSSR AVTNSPVVVA LDYHNRDDAL AFVDKIDPRD CRLKVGKEMF TLFGPQFVRE LQQRGFDIFL
0071 DLKFHDIPNT AAHAVAAAAD LGVWMVNVHA SGGARMMTAA REALVPFGKD APLLIAVTVL TSMEASDLVD
0141 LGMTLSPADY AERLAALTQK CGLDGVVCSA QEAVRFKQVF GQEFKLVTPG IRPQGSEAGD QRRIMTPEQA
0211 LSAGVDYMVI GRPVTQSVDP AQTLKAINAS LQRSA
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 GAGAAATCGC AACTGTTAAT TTTTTATTTC
-070 CACGGGTAGA ATGCTCGCCG TTTACCTGTT TCGCGCCACT TCCGGTGCCC ATCATCAAGA AGGTCTGGTC
0001 atgacgttaa ctgcttcatc ttcttcccgc gctgttacga attctcctgt ggttgttgcc cttgattatc
0071 ataatcgtga tgacgcgctg gcctttgtcg acaagatcga cccacgcgat tgtcgtctga aggtcggcaa
0141 agagatgttt acattgtttg ggccacagtt tgtgcgcgaa cttcaacagc gtggttttga tatctttctt
0211 gacctgaaat tccacgatat ccccaacact gcagcgcacg ctgtcgctgc tgcagctgac ttaggcgtgt
0281 ggatggtgaa tgttcatgcc tctggtgggg cgcgtatgat gaccgcagcg cgtgaggcac tggttccgtt
0351 tggcaaagat gcaccgcttt tgattgctgt gacagtgttg accagcatgg aagccagcga cctggtcgat
0421 cttggcatga cactgtcacc tgcagattat gcagaacgtc tggcggcact gacgcaaaaa tgtggccttg
0491 atggtgtggt gtgttctgct caggaagctg tgcgctttaa acaggtattc ggtcaggagt tcaaactggt
0561 tacgccgggc attcgtccgc aggggagtga agctggtgac cagcgccgca ttatgacgcc agaacaggcg
0631 ttgtcggctg gtgttgatta tatggtgatt ggtcgcccgg taacgcaatc ggtagatcca gcgcagacgc
0701 tgaaagcgat caacgcctct ttacagcgga gtgcatgaTG AGTGATTCCA ACAGCCGTCT GGTCTACTCA
0771 ACGGAAACCG GACGTATTGA TGAGCCCAAA GCGGCCCCTG TACGCCCTAA AGGCGACGGT GTGGTGCG
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