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PEC Original Annotations
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Essentiality
|
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Class
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non-essential
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|
References (PMID)
|
Microbiology. 1996;142 (Pt 2):389-400.
The second aconitase (AcnB) of Escherichia coli.
Bradbury AJ, Gruer MJ, Rudd KE, Guest JR.
(
8932712
)
|
|
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Deletion
|
OCR69-7
(D)
|
|
Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
acnA
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Related strains
|
|
Strains Search
|
Search strains by gene name:
acnA
Search strains by all related name:
acnA acn b1276 ECK1271 JW1268
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General information (Go to Linear View: )
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Gene Name
|
acnA
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|
Alternative name
|
acn,b1276,ECK1271,JW1268
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Location, Length
|
1,333,855 - 1,336,530
(
+
)
;
28.75
min
;
2676
(bp) ,
891
(aa)
|
|
Product
|
aconitate hydratase 1
|
|
Operon Name
|
acnA
|
|
Note
|
aconitate hydrase 1; GO_process: GO:0006099 - tricarboxylic acid cycle; GO_process: GO:0009061 - anaerobic respiration
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Function
|
enzyme; Energy metabolism, carbon: TCA cycle
|
|
Gene Ontology
|
GO:0003994
;
aconitate hydratase activity ( acnA )
GO:0005506
;
iron ion binding ( acnA )
GO:0005515
;
protein binding ( acnA )
GO:0006099
;
tricarboxylic acid cycle ( acnA )
GO:0008152
;
metabolic process ( acnA )
GO:0016829
;
lyase activity ( acnA )
GO:0046872
;
metal ion binding ( acnA )
GO:0051536
;
iron-sulfur cluster binding ( acnA )
GO:0051539
;
4 iron, 4 sulfur cluster binding ( acnA )
|
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PID
|
1787531
|
EC number
(KEGG Pathway)
|
4.2.1.3
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
acnA
(
EG11325
)
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Number of removed
|
1 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
ACO1_ECOLI
(
P25516
;
P78060; P78148
)
|
|
- Protein name
|
Aconitate hydratase 1
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- Synonyms
|
EC 4.2.1.3, Citrate hydro-lyase 1, Aconitase 1
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- Gene name
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Name=acnA; Synonyms=acn; OrderedLocusNames=b1276;
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Homology Analysis
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BLAST
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Bacteria
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|
GTOP
|
acnA
(
homologous genes of other bacterias
)
|
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PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Structure Of An Orthorhombic Crystal Form Of Human Cytosolic Aconitase (Irp1)
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|
SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Aconitate hydratase 1
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PSI-BLAST
|
Aconitate hydratase 1
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|
nr
(database updated :
2007.02.20
)
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BLAST
|
aconitate hydratase [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
PROKAR_LIPOPROTEIN
ACONITASE_1
ACONITASE_2
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MMBR References
|
J Gen Microbiol. 1991;137(11):2505-15.
The aconitase of Escherichia coli: purification of the enzyme and molecular cloning and map location of the gene (acn).
Prodromou C, Haynes MJ, Guest JR.
(
1838390
)
|
Eur J Biochem. 1992;204:599-609
The aconitase of Escherichia coli: nucleotide sequence of the aconitase gene and amino acid sequence similarity with mitochondrial aconitases, the iron-responsive-element-binding protein and isopropylmalate isomerase.
Prodromou, C., P. J. Artymiuk, and J. R. Guest.
|
| Sequences |
Amino acid
FASTA format
|
0001 MSSTLREASK DTLQAKDKTY HYYSLPLAAK SLGDITRLPK SLKVLLENLL RWQDGNSVTE EDIHALAGWL
0071 KNAHADREIA YRPARVLMQD FTGVPAVVDL AAMREAVKRL GGDTAKVNPL SPVDLVIDHS VTVDRFGDDE
0141 AFEENVRLEM ERNHERYVFL KWGKQAFSRF SVVPPGTGIC HQVNLEYLGK AVWSELQDGE WIAYPDTLVG
0211 TDSHTTMING LGVLGWGVGG IEAEAAMLGQ PVSMLIPDVV GFKLTGKLRE GITATDLVLT VTQMLRKHGV
0281 VGKFVEFYGD GLDSLPLADR ATIANMSPEY GATCGFFPID AVTLDYMRLS GRSEDQVELV EKYAKAQGMW
0351 RNPGDEPIFT STLELDMNDV EASLAGPKRP QDRVALPDVP KAFAASNELE VNATHKDRQP VDYVMNGHQY
0421 QLPDGAVVIA AITSCTNTSN PSVLMAAGLL AKKAVTLGLK RQPWVKASLA PGSKVVSDYL AKAKLTPYLD
0491 ELGFNLVGYG CTTCIGNSGP LPDPIETAIK KSDLTVGAVL SGNRNFEGRI HPLVKTNWLA SPPLVVAYAL
0561 AGNMNINLAS EPIGHDRKGD PVYLKDIWPS AQEIARAVEQ VSTEMFRKEY AEVFEGTAEW KGINVTRSDT
0631 YGWQEDSTYI RLSPFFDEMQ ATPAPVEDIH GARILAMLGD SVTTDHISPA GSIKPDSPAG RYLQGRGVER
0701 KDFNSYGSRR GNHEVMMRGT FANIRIRNEM VPGVEGGMTR HLPDSDVVSI YDAAMRYKQE QTPLAVIAGK
0771 EYGSGSSRDW AAKGPRLLGI RVVIAESFER IHRSNLIGMG ILPLEFPQGV TRKTLGLTGE EKIDIGDLQN
0841 LQPGATVPVT LTRADGSQEV VPCRCRIDTA TELTYYQNDG ILHYVIRNML K
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ATTTGGGTTG TTATCAAATC GTTACGCGAT
-070 GTTTGTGTTA TCTTTAATAT TCACCCTGAA GAGAATCAGG GCTTCGCAAC CCTGTCATTA AGGAGGAGCT
0001 atgtcgtcaa ccctacgaga agccagtaag gacacgttgc aggccaaaga taaaacttac cactactaca
0071 gcctgccgct tgctgctaaa tcactgggcg atatcacccg tctacccaag tcactcaaag ttttgctcga
0141 aaacctgctg cgctggcagg atggtaactc ggttaccgaa gaggatatcc acgcgctggc aggatggctg
0211 aaaaatgccc atgctgaccg tgaaattgcc taccgcccgg caagggtgct gatgcaggac tttaccggcg
0281 tacctgccgt tgttgatctg gcggcaatgc gcgaagcggt taaacgcctc ggcggcgata ctgcaaaggt
0351 taacccgctc tcaccggtcg acctggtcat tgaccactcg gtgaccgtcg atcgttttgg tgatgatgag
0421 gcatttgaag aaaacgtacg cctggaaatg gagcgcaacc acgaacgtta tgtgttcctg aaatggggaa
0491 agcaagcgtt cagtcggttt agcgtcgtgc cgccaggcac aggcatttgc catcaggtta acctcgaata
0561 tctcggcaaa gcagtgtgga gtgaattgca ggacggtgaa tggattgctt atccggatac actcgttggt
0631 actgactcgc acaccaccat gatcaacggc cttggcgtgc tggggtgggg cgttggtggg atcgaagcag
0701 aagccgcaat gttaggccag ccggtttcca tgcttatccc ggatgtagtg ggcttcaaac ttaccggaaa
0771 attacgtgaa ggtattaccg ccacagacct ggttctcact gttacccaaa tgctgcgcaa acatggcgtg
0841 gtggggaaat tcgtcgaatt ttatggtgat ggtctggatt cactaccgtt ggcggatcgc gccaccattg
0911 ccaatatgtc gccagaatat ggtgccacct gtggcttctt cccaatcgat gctgtaaccc tcgattacat
0981 gcgtttaagc gggcgcagcg aagatcaggt cgagttggtc gaaaaatatg ccaaagcgca gggcatgtgg
1051 cgtaacccgg gcgatgaacc aatttttacc agtacgttag aactggatat gaatgacgtt gaagcgagcc
1121 tggcagggcc taaacgccca caggatcgcg ttgcactgcc cgatgtacca aaagcatttg ccgccagtaa
1191 cgaactggaa gtgaatgcca cgcataaaga tcgccagccg gtcgattatg ttatgaacgg acatcagtat
1261 cagttacctg atggcgctgt ggtcattgct gcgataacct cgtgcaccaa cacctctaac ccaagtgtgc
1331 tgatggccgc aggcttgctg gcgaaaaaag ccgtaactct gggcctcaag cggcaaccat gggtcaaagc
1401 gtcgctggca ccgggttcga aagtcgtttc tgattatctg gcaaaagcga aactgacacc gtatctcgac
1471 gaactggggt ttaaccttgt gggatacggt tgtaccacct gtattggtaa ctctgggccg ctgcccgatc
1541 ctatcgaaac ggcaatcaaa aaaagcgatt taaccgtcgg tgcggtgctg tccggcaacc gtaactttga
1611 aggccgtatc catccgctgg ttaaaactaa ctggctggcc tcgccgccgc tggtggttgc ctatgcgctg
1681 gcgggaaata tgaatatcaa cctggcttct gagcctatcg gccatgatcg caaaggcgat ccggtttatc
1751 tgaaagatat ctggccatcg gcacaagaaa ttgcccgtgc ggtagaacaa gtctccacag aaatgttccg
1821 caaagagtac gcagaagttt ttgaaggcac agcagagtgg aagggaatta acgtcacacg atccgatacc
1891 tacggttggc aggaggactc aacctatatt cgcttatcgc ctttctttga tgaaatgcag gcaacaccag
1961 caccagtgga agatattcac ggtgcgcgga tcctcgcaat gctgggggat tcagtcacca ctgaccatat
2031 ctctccggcg ggcagtatta agcccgacag cccagcgggt cgatatctac aaggtcgggg tgttgagcga
2101 aaagacttta actcctacgg ttcgcggcgt ggtaaccatg aagtgatgat gcgcggcacc ttcgccaata
2171 ttcgcatccg taatgaaatg gtgcctggcg ttgaaggggg gatgacgcgg catttacctg acagcgacgt
2241 agtctctatt tatgatgctg cgatgcgcta taagcaggag caaacgccgc tggcggtgat tgccgggaaa
2311 gagtatggat caggctccag tcgtgactgg gcggcaaaag gtccgcgtct gcttggtatt cgtgtggtga
2381 ttgccgaatc gtttgaacga attcaccgtt cgaatttaat tggcatgggc atcctgccgc tggaatttcc
2451 gcaaggcgta acgcgtaaaa cgttagggct aaccggggaa gagaagattg atattggcga tctgcaaaac
2521 ctacaacccg gcgcgacggt tccggtgacg cttacgcgcg cggatggtag ccaggaagtc gtaccctgcc
2591 gttgtcgtat cgacaccgcg acggagttga cctactacca gaacgacggc attttgcatt atgtcattcg
2661 taatatgttg aagtaaCAAC TATTTGCTTG CCGGTTATTT TGCTTCCGGC AAGCAAATGA ATTACACAAT
2731 GCAAGAGGGT TATTTGTTCA GCAAATGGCC CATTTTCTCG GCTTTG
|
