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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Proc Natl Acad Sci U S A. 1987;84(24):8971-5.
Expression of yeast DNA topoisomerase I can complement a conditional-lethal DNA topoisomerase I mutation in Escherichia coli.
Bjornsti MA, Wang JC.
(
2827163
)
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Deletion
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DE(tonB-cysB)206
,
OCR68,69-2
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
topA
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Related strains
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Strains Search
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Search strains by gene name:
topA
Search strains by all related name:
topA asuA? b1274 ECK1268 JW1266 supX
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General information (Go to Linear View: )
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Gene Name
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topA
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Alternative name
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asuA?,b1274,ECK1268,JW1266,supX
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Location, Length
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1,329,072 - 1,331,669
(
+
)
;
28.65
min
;
2598
(bp) ,
865
(aa)
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Product
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DNA topoisomerase I, omega subunit
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Operon Name
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topA
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Note
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DNA topoisomerase type I, omega protein; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006261 - DNA-dependent DNA replication; GO_process: GO:0006350 - transcription
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Function
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enzyme; DNA - replication, repair,restriction/modification
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Gene Ontology
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GO:0003676
;
nucleic acid binding ( topA )
GO:0003677
;
DNA binding ( topA )
GO:0003916
;
DNA topoisomerase activity ( topA )
GO:0003917
;
DNA topoisomerase type I activity ( topA )
GO:0003918
;
DNA topoisomerase (ATP-hydrolyzing) activity ( topA )
GO:0005515
;
protein binding ( topA )
GO:0005694
;
chromosome ( topA )
GO:0006265
;
DNA topological change ( topA )
GO:0006268
;
DNA unwinding during replication ( topA )
GO:0006304
;
DNA modification ( topA )
GO:0008270
;
zinc ion binding ( topA )
GO:0016853
;
isomerase activity ( topA )
GO:0046872
;
metal ion binding ( topA )
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PID
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1787529
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EC number
(KEGG Pathway)
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5.99.1.2
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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topA
(
EG11013
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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TOP1_ECOLI
(
P06612
)
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- Protein name
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DNA topoisomerase I
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- Synonyms
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EC 5.99.1.2, Omega-protein, Relaxing enzyme, Untwisting enzyme, Swivelase
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- Gene name
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Name=topA; Synonyms=supX; OrderedLocusNames=b1274;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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topA
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, Complex Of E.Coli Dna Topoisomerase I With 5'ptptpt
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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DNA topoisomerase 1
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PSI-BLAST
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DNA topoisomerase 1
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nr
(database updated :
2007.02.20
)
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BLAST
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DNA topoisomerase I [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
AMIDATION
TOPOISOMERASE_I_PROK
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MMBR References
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Mol Gen Genet. 1972;117(2):91-112.
Suppression of promoter mutations by the pleiotropic supx mutations.
Dubnau E, Margolin P.
(
4561426
)
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J Bacteriol. 1980;143(2):747-52.
Locations of the opp and supX genes of Salmonella typhimurium and Escherichia coli.
Lenny AB, Margolin P.
(
7009564
)
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J Bacteriol. 1993;175:1645-1655
Anchoring of DNA to the bacterial cytoplasmic membrane through cotranscriptional synthesis of polypeptides encoding membrane proteins or proteins for export: a mechanism of plasmid hypernegative supercoiling in mutants deficient in DNA topoisomerase I.
Lynch, A. S., and J. C. Wang.
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Proc Natl Acad Sci U S A. 1985;82:5437-5441
The Escherichia coli supX locus in topA, the structural gene for DNA topoisomerase I.
Margolin, P., L. Zumstein, R. Sternglanz, and J. C. Wang.
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J Bacteriol. 1992;174:4175-4178
An Escherichia coli DNA topoisomerase I mutant has a compensatory mutation that alters two residues between functional domains of the DNA gyrase A protein.
Oram, M., and L. M. Fisher.
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J Biol Chem. 1987;262:5999-6005
DNA sequences of the cysB regions of Salmonella typhimurium and Escherichia coli.
Ostrowski, J., G. Jagura-Burdzy, and N. M. Kredich.
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Proc Natl Acad Sci U S A. 1981;78:2747-2751
Mutations in the gene coding for Escherichia coli DNA topoisomerase I affect transcription and translation.
Sternglanz, R., S. DiNardo, K. A. Voelkel, Y. Nishimura, Y. Hirota, K. Becherer, L. Zumstein, and J. C. Wang.
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J Bacteriol. 1981;147(2):679-81.
Escherichia coli and Salmonella typhimurium supX genes specify deoxyribonucleic acid topoisomerase I.
Trucksis M, Golub EI, Zabel DJ, Depew RE.
(
6267017
)
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Proc Natl Acad Sci U S A. 1981;78:2164-2168
Identification and localization of a gene that specifies production of Escherichia coli DNA topoisomerase.
Trucksis, M., and R. E. Depew.
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J Mol Biol. 1986;191(3):321-31.
Complete nucleotide sequence of the topA gene encoding Escherichia coli DNA topoisomerase I.
Tse-Dinh YC, Wang JC.
(
3029379
)
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Nucleic Acids Res. 1983;11:1773-1790
Cloning of the gene topA encoding for DNA topoisomerase I and the physical mapping of the cysB-topA-trp region of Escherichia coli.
Wang, J. C., and K. Becherer.
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| Sequences |
Amino acid
FASTA format
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0001 MGKALVIVES PAKAKTINKY LGSDYVVKSS VGHIRDLPTS GSAAKKSADS TSTKTAKKPK KDERGALVNR
0071 MGVDPWHNWE AHYEVLPGKE KVVSELKQLA EKADHIYLAT DLDREGEAIA WHLREVIGGD DARYSRVVFN
0141 EITKNAIRQA FNKPGELNID RVNAQQARRF MDRVVGYMVS PLLWKKIARG LSAGRVQSVA VRLVVERERE
0211 IKAFVPEEFW EVDASTTTPS GEALALQVTH QNDKPFRPVN KEQTQAAVSL LEKARYSVLE REDKPTTSKP
0281 GAPFITSTLQ QAASTRLGFG VKKTMMMAQR LYEAGYITYM RTDSTNLSQD AVNMVRGYIS DNFGKKYLPE
0351 SPNQYASKEN SQEAHEAIRP SDVNVMAESL KDMEADAQKL YQLIWRQFVA CQMTPAKYDS TTLTVGAGDF
0421 RLKARGRILR FDGWTKVMPA LRKGDEDRIL PAVNKGDALT LVELTPAQHF TKPPARFSEA SLVKELEKRG
0491 IGRPSTYASI ISTIQDRGYV RVENRRFYAE KMGEIVTDRL EENFRELMNY DFTAQMENSL DQVANHEAEW
0561 KAVLDHFFSD FTQQLDKAEK DPEEGGMRPN QMVLTSIDCP TCGRKMGIRT ASTGVFLGCS GYALPPKERC
0631 KTTINLVPEN EVLNVLEGED AETNALRAKR RCPKCGTAMD SYLIDPKRKL HVCGNNPTCD GYEIEEGEFR
0701 IKGYDGPIVE CEKCGSEMHL KMGRFGKYMA CTNEECKNTR KILRNGEVAP PKEDPVPLPE LPCEKSDAYF
0771 VLRDGAAGVF LAANTFPKSR ETRAPLVEEL YRFRDRLPEK LRYLADAPQQ DPEGNKTMVR FSRKTKQQYV
0841 SSEKDGKATG WSAFYVDGKW VEGKK
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 ATATCCGCAG AGAGCGAGTC CATATCGGTA
-070 ACTCGTTGCC AGTGGAAGGT TTATCAACGT GCGACGCATT CCTGGAAGAA TCAAATTAGG TAAGGTGAAT
0001 atgggtaaag ctcttgtcat cgttgagtcc ccggcaaaag ccaaaacgat caacaagtat ctgggtagtg
0071 actacgtggt gaaatccagc gtcggtcaca tccgcgattt gccgaccagt ggctcagctg ccaaaaagag
0141 tgccgactct acctccacca agacggctaa aaagcctaaa aaggatgaac gtggcgctct cgtcaaccgt
0211 atgggggttg acccgtggca caattgggag gcgcactatg aagtgttgcc tggtaaagag aaggtcgtct
0281 ctgaactgaa acaactggct gaaaaagccg accacatcta tctcgcaacc gaccttgacc gcgaagggga
0351 agccattgca tggcacctgc gggaagtgat tgggggtgat gatgcgcgct atagccgagt ggtgtttaac
0421 gaaattacta aaaacgcgat ccgccaggca tttaacaaac cgggtgagct gaatattgat cgtgttaatg
0491 cccagcaggc gcgtcgcttt atggaccgcg tggtggggta tatggtttcg ccgctgctat ggaaaaagat
0561 cgctcgtggc ctgtctgccg gtcgtgtgca gtcggtggcg gttcgcctgg tggtcgagcg tgagcgtgaa
0631 attaaagcgt tcgtgccgga agagttctgg gaagtcgatg ccagcacgac cacgccatct ggtgaagcgt
0701 tggcgttaca ggtgactcat cagaacgaca aaccgttccg tccggtcaac aaagaacaaa ctcaggctgc
0771 ggtaagtctg ctggaaaaag cgcgctacag cgtgctggaa cgtgaagaca aaccgacaac cagtaaacct
0841 ggcgctcctt ttattacctc tacgctgcaa caagctgcca gcacccgtct tggatttggc gtgaaaaaaa
0911 ccatgatgat ggcgcagcgt ttgtatgaag caggctatat cacttacatg cgtaccgact ccactaacct
0981 gagtcaggac gcggtaaata tggttcgcgg ttatatcagc gataattttg gtaagaaata tctgccggaa
1051 agtccgaatc agtacgccag caaagaaaac tcacaggaag cgcacgaagc gattcgtcct tctgacgtca
1121 atgtgatggc ggaatcgctg aaggatatgg aagcagatgc gcagaaactg taccagttaa tctggcgtca
1191 gttcgttgcc tgccagatga ccccagcgaa atatgactcc acgacgctga ccgttggtgc gggcgatttc
1261 cgcctgaaag cacgcggtcg tattttgcgt tttgatggct ggacaaaagt gatgcctgcg ttgcgtaaag
1331 gcgatgaaga tcgcatctta ccagcagtta ataaaggcga tgctctgacg ctcgttgaac ttacaccagc
1401 ccagcacttt accaagccgc cagcccgttt cagtgaagca tcgctggtta aagagctgga aaaacgcggt
1471 atcggtcgtc cgtctaccta tgcgtcgatc atttcgacca ttcaggatcg tggctacgtg cgagtagaaa
1541 atcgtcgttt ctatgcggaa aaaatgggcg aaatcgtcac cgatcgcctt gaagaaaatt tccgcgagtt
1611 aatgaactac gactttaccg cgcagatgga aaacagcctc gaccaggtgg caaatcacga agcagagtgg
1681 aaagctgtac tggatcactt cttctcggat ttcacccagc agttagataa agctgaaaaa gatccggaag
1751 agggtggtat gcgcccgaac cagatggttc tgaccagcat tgactgcccg acttgtggtc gcaaaatggg
1821 gattcgcaca gcgagcaccg gggtattcct tggctgttct ggctatgcgc tgccgccgaa agagcgttgc
1891 aaaaccacca ttaacctggt gccggaaaac gaagtgctga acgtgctgga aggcgaagat gctgaaacca
1961 acgcgctgcg cgcaaaacgt cgttgcccga aatgcggcac ggcgatggac agctatctca tcgatccgaa
2031 acgtaagttg catgtctgtg gtaataaccc aacctgcgac ggttacgaga tcgaagaggg cgaattccgc
2101 attaaaggtt atgacggccc gatcgttgag tgtgaaaaat gtggctctga aatgcacctg aaaatggggc
2171 gtttcggtaa atacatggcc tgcaccaacg aagagtgtaa aaacacacgt aagattttac gtaacggcga
2241 agtggcacca ccgaaagaag atccggtgcc attacctgag ctgccgtgcg aaaaatcaga tgcttatttc
2311 gtgctgcgtg acggtgctgc cggtgtgttc ctggctgcca acactttccc gaaatcgcgt gaaacgcgtg
2381 cgccactggt ggaagagctt tatcgcttcc gcgaccgtct gccggaaaaa ctgcgttatc tggccgatgc
2451 gccacagcag gatccggaag gtaataagac catggttcgc tttagccgta aaaccaaaca gcaatatgtc
2521 tcttcggaaa aagacggaaa ggcgactggc tggtcagcat tttatgttga tggcaaatgg gttgaaggaa
2591 aaaaataaCC TTTAATTCTG TCAGGTTTTT ATAAACAAAG GGTCGCGAAA GCGGCCCTTT TTTATTGCAT
2661 ATTATTTTTT CTTCACACCT ATACACTAAG GCTATAAA
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