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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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DE(tonB-cysB)206
,
OCR68,69-1
(D)
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Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
trpB
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Related strains
|
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Strains Search
|
Search strains by gene name:
trpB
Search strains by all related name:
trpB b1261 ECK1255 JW1253
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General information (Go to Linear View: )
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Gene Name
|
trpB
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Alternative name
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b1261,ECK1255,JW1253
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Location, Length
|
1,315,246 - 1,316,439
(
-
)
;
28.35
min
;
1194
(bp) ,
397
(aa)
|
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Product
|
tryptophan synthase, beta subunit
|
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Operon Name
|
trpLEDCBA
|
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Note
|
tryptophan synthase, beta protein; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0000162 - tryptophan biosynthetic process
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Function
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enzyme; Amino acid biosynthesis: Tryptophan
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Gene Ontology
|
GO:0000162
;
tryptophan biosynthetic process ( trpB )
GO:0003824
;
catalytic activity ( trpB )
GO:0004834
;
tryptophan synthase activity ( trpB )
GO:0006568
;
tryptophan metabolic process ( trpB )
GO:0008152
;
metabolic process ( trpB )
GO:0008652
;
amino acid biosynthetic process ( trpB )
GO:0009073
;
aromatic amino acid family biosynthetic process ( trpB )
GO:0016829
;
lyase activity ( trpB )
GO:0030170
;
pyridoxal phosphate binding ( trpB )
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PID
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1787515
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EC number
(KEGG Pathway)
|
4.2.1.20
|
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
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EcoGene
|
trpB
(
EG11025
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
|
TRPB_ECOLI
(
P00932
;
P78146
)
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- Protein name
|
Tryptophan synthase beta chain
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- Synonyms
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EC 4.2.1.20
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- Gene name
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Name=trpB; OrderedLocusNames=b1261, SF1264, S1350;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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trpB
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain B, Tryptophan Synthase (E.C.4.2.1.20) In The Presence Of Cesium, Room Temperature
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Tryptophan synthase beta chain
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PSI-BLAST
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Tryptophan synthase beta chain
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nr
(database updated :
2007.02.20
)
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BLAST
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tryptophan synthase subunit beta [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
GLYCOSAMINOGLYCAN
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
RGD
TRP_SYNTHASE_BETA
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MMBR References
|
Gene. 1980;9(1-2):69-85.
Construction of new cloning vehicles with genes of the tryptophan operon of Escherichia coli as genetic markers.
Enger-Valk BE, Heyneker HL, Oosterbaan RA, Pouwels PH.
(
6247248
)
|
J Mol Biol. 1969;44(1):117-27.
Directed transposition of the arabinose operon: a technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene.
Gottesman S, Beckwith JR.
(
4897798
)
|
J Mol Biol. 1969;39(1):159-79.
Operator mutants of the tryptophan operon in Escherichia coli.
Hiraga S.
(
4938813
)
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J Bacteriol. 1971;107(1):8-15.
Regulation of tyrosine biosynthesis in Escherichia coli K-12: isolation and characterization of operator mutants.
Mattern IE, Pittard J.
(
4397929
)
|
Nucleic Acids Res. 1981;9:6647-6668
The complete nucleotide sequence of the tryptophan operon of E. coli.
Yanofsky, C., T. Platt, I. P. Crawford, B. Nichols, G. E. Christie, H. Horowitz, M. VanCleemput, and A. M. Wu.
|
J Mol Biol. 1980;142(4):489-502.
Nucleotide sequence of the trpB gene in Escherichia coli and Salmonella typhimurium.
Crawford IP, Nichols BP, Yanofsky C.
(
7007651
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MTTLLNPYFG EFGGMYVPQI LMPALRQLEE AFVSAQKDPE FQAQFNDLLK NYAGRPTALT KCQNITAGTN
0071 TTLYLKREDL LHGGAHKTNQ VLGQALLAKR MGKTEIIAET GAGQHGVASA LASALLGLKC RIYMGAKDVE
0141 RQSPNVFRMR LMGAEVIPVH SGSATLKDAC NEALRDWSGS YETAHYMLGT AAGPHPYPTI VREFQRMIGE
0211 ETKAQILERE GRLPDAVIAC VGGGSNAIGM FADFINETNV GLIGVEPGGH GIETGEHGAP LKHGRVGIYF
0281 GMKAPMMQTE DGQIEESYSI SAGLDFPSVG PQHAYLNSTG RADYVSITDD EALEAFKTLC LHEGIIPALE
0351 SSHALAHALK MMRENPDKEQ LLVVNLSGRG DKDIFTVHDI LKARGEI
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ttgattttaa ttctgctgta gagtcgcaac
-070 cgggcatcaa agacgcacgt cttttggcct cggttttcca gacgctgcgc gcatattaag gaaaggaaca
0001 atgacaacat tacttaaccc ctattttggt gagtttggcg gcatgtacgt gccacaaatc ctgatgcctg
0071 ctctgcgcca gctggaagaa gcttttgtca gtgcgcaaaa agatcctgaa tttcaggctc agttcaacga
0141 cctgctgaaa aactatgccg ggcgtccaac cgcgctgacc aaatgccaga acattacagc cgggacgaac
0211 accacgctgt atctcaagcg tgaagatttg ctgcacggcg gcgcgcataa aactaaccag gtgctggggc
0281 aggcgttgct ggcgaagcgg atgggtaaaa ccgaaatcat cgccgaaacc ggtgccggtc agcatggcgt
0351 ggcgtcggcc cttgccagcg ccctgctcgg cctgaaatgc cgtatttata tgggtgccaa agacgttgaa
0421 cgccagtcgc ctaacgtttt tcgtatgcgc ttaatgggtg cggaagtgat cccggtgcat agcggttccg
0491 cgacgctgaa agatgcctgt aacgaggcgc tgcgcgactg gtccggtagt tacgaaaccg cgcactatat
0561 gctgggcacc gcagctggcc cgcatcctta tccgaccatt gtgcgtgagt ttcagcggat gattggcgaa
0631 gaaaccaaag cgcagattct ggaaagagaa ggtcgcctgc cggatgccgt tatcgcctgt gttggcggcg
0701 gttcgaatgc catcggcatg tttgctgatt tcatcaatga aaccaacgtc ggcctgattg gtgtggagcc
0771 aggtggtcac ggtatcgaaa ctggcgagca cggcgcaccg ctaaaacatg gtcgcgtggg tatctatttc
0841 ggtatgaaag cgccgatgat gcaaaccgaa gacgggcaga ttgaagaatc ttactccatc tccgccggac
0911 tggatttccc gtctgtcggc ccacaacacg cgtatcttaa cagcactgga cgcgctgatt acgtgtctat
0981 taccgatgat gaagcccttg aagccttcaa aacgctgtgc ctgcacgaag ggatcatccc ggcgctggaa
1051 tcctcccacg ccctggccca tgcgttgaaa atgatgcgcg aaaacccgga taaagagcag ctactggtgg
1121 ttaacctttc cggtcgcggc gataaagaca tcttcaccgt tcacgatatt ttgaaagcac gaggggaaat
1191 ctgatggaac gctacgaatc tctgtttgcc cagttgaagg agcgcaaaga aggcgcattc gttcctttcg
1261 tcacgctcgg tgatccgggc attgagcagt catt
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