MG1655
W3110
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Gene Report : trpA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion DE(tonB-cysB)206  ,  OCR68,69-1 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  trpA
Related strains
     Strains Search Search strains by gene name:  trpA   Search strains by all related name:  trpA b1260 ECK1254 JW1252 try tryp

General information  (Go to Linear View:)
 Gene Name trpA  
 Alternative name b1260,ECK1254,JW1252,try,tryp  
 Location, Length 1,314,440 - 1,315,246 (  -  ) ;   28.33 min ; 807 (bp) ,   268 (aa) Go to Linear View
 Product tryptophan synthase, alpha subunit  
 Operon Name trpLEDCBA  
 Note tryptophan synthase, alpha protein; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0000162 - tryptophan biosynthetic process  
 Function enzyme; Amino acid biosynthesis: Tryptophan  
 Gene Ontology GO:0000162; tryptophan biosynthetic process ( trpA )
GO:0003824; catalytic activity ( trpA )
GO:0004834; tryptophan synthase activity ( trpA )
GO:0006568; tryptophan metabolic process ( trpA )
GO:0008152; metabolic process ( trpA )
GO:0008652; amino acid biosynthetic process ( trpA )
GO:0009073; aromatic amino acid family biosynthetic process ( trpA )
GO:0016829; lyase activity ( trpA )
 PID 1787514  
 EC number
  (KEGG Pathway)
 
4.2.1.20  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene trpA ( EG11024 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) TRPA_ECOLI ( P00928 ; Q47669 )
    -  Protein name Tryptophan synthase alpha chain  
    -  Synonyms EC 4.2.1.20  
    -  Gene name Name=trpA; OrderedLocusNames=b1260, SF1263, S1349;  

 Linear View (Whole Mode)
View Location
   1305.0  –  1330.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP trpA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Wild-Type Tryptophan Synthase Alpha- Subunits From Escherichia Coli  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Tryptophan synthase alpha chain  
         PSI-BLAST Tryptophan synthase alpha chain
    nr     (database updated : 2007.02.20 )
         BLAST tryptophan synthase subunit alpha [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    TRP_SYNTHASE_ALPHA     

Other Cross-Reference
    COG COG0159E 
    EcoCyc trpA 

MMBR References
Gene. 1980;9(1-2):69-85.
Construction of new cloning vehicles with genes of the tryptophan operon of Escherichia coli as genetic markers.
Enger-Valk BE, Heyneker HL, Oosterbaan RA, Pouwels PH. ( 6247248 )
J Mol Biol. 1969;44(1):117-27.
Directed transposition of the arabinose operon: a technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene.
Gottesman S, Beckwith JR. ( 4897798 )
J Biol Chem. 1967;242:5442-5446
The amino acid sequence of the A protein (alpha subunit) of the tryptophan synthetase of Escherichia coli.
Guest, J. R., G. R. Drapeau, B. C. Carlton, and C. Yanofsky.
J Mol Biol. 1969;39(1):159-79.
Operator mutants of the tryptophan operon in Escherichia coli.
Hiraga S. ( 4938813 )
J Bacteriol. 1971;107(1):8-15.
Regulation of tyrosine biosynthesis in Escherichia coli K-12: isolation and characterization of operator mutants.
Mattern IE, Pittard J. ( 4397929 )
Proc Natl Acad Sci U S A. 1979;76:5244-5248
Nucleotide sequences of trpA of Salmonella typhimurium and Escherichia coli: an evolutionary comparison.
Nichols, B., and C. Yanofsky.
Mol Gen Genet. 1992;233(1-2):129-35.
Selection and analysis of non-interactive mutants in the Escherichia coli tryptophan synthase alpha subunit.
Swift S, Kuhn J, Stewart GS. ( 1603055 )
Cell. 1980;19(4):829-36.
Deletions of distal sequence after termination of transcription at the end of the tryptophan operon in E. coli.
Wu AM, Chapman AB, Platt T, Guarente LP, Beckwith J. ( 6991123 )
Proc Natl Acad Sci U S A. 1981;78:2913-2917
Tandem termination sites in the tryptophan operon of Escherichia coli.
Wu, A. M., G. E. Christie, and T. Platt.
Nucleic Acids Res. 1981;9:6647-6668
The complete nucleotide sequence of the tryptophan operon of E. coli.
Yanofsky, C., T. Platt, I. P. Crawford, B. Nichols, G. E. Christie, H. Horowitz, M. VanCleemput, and A. M. Wu.

Sequences
Amino acid
FASTA format
0001 MERYESLFAQ LKERKEGAFV PFVTLGDPGI EQSLKIIDTL IEAGADALEL GIPFSDPLAD GPTIQNATLR 
0071 AFAAGVTPAQ CFEMLALIRQ KHPTIPIGLL MYANLVFNKG IDEFYAQCEK VGVDSVLVAD VPVEESAPFR 
0141 QAALRHNVAP IFICPPNADD DLLRQIASYG RGYTYLLSRA GVTGAENRAA LPLNHLVAKL KEYNAAPPLQ 
0211 GFGISAPDQV KAAIDAGAAG AISGSAIVKI IEQHINEPEK MLAALKVFVQ PMKAATRS

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             acccggataa agagcagcta ctggtggtta 
-070 acctttccgg tcgcggcgat aaagacatct tcaccgttca cgatattttg aaagcacgag gggaaatctg 
0001 atggaacgct acgaatctct gtttgcccag ttgaaggagc gcaaagaagg cgcattcgtt cctttcgtca 
0071 cgctcggtga tccgggcatt gagcagtcat tgaaaattat cgatacgcta attgaagccg gtgctgacgc 
0141 gctggagtta ggtatcccct tctccgaccc actggcggat ggcccgacga ttcaaaacgc cactctgcgc 
0211 gcctttgcgg caggtgtgac tccggcacaa tgttttgaaa tgctggcact gattcgccag aaacacccga 
0281 ccattcccat tggcctgttg atgtatgcca atctggtgtt taacaaaggc attgatgagt tttatgccca 
0351 gtgcgaaaaa gtcggcgtcg attcggtgct ggttgccgat gtgccagttg aagagtccgc gcccttccgc 
0421 caggccgcgt tgcgtcataa tgtcgcacct atcttcatct gcccgccaaa tgccgatgac gacctgctgc 
0491 gccagatagc ctcttacggt cgtggttaca cctatttgct gtcacgagca ggcgtgaccg gcgcagaaaa 
0561 ccgcgccgcg ttacccctca atcatctggt tgcgaagctg aaagagtaca acgctgcacc tccattgcag 
0631 ggatttggta tttccgcccc ggatcaggta aaagcagcga ttgatgcagg agctgcgggc gcgatttctg 
0701 gttcggccat tgttaaaatc atcgagcaac atattaatga gccagagaaa atgctggcgg cactgaaagt 
0771 ttttgtacaa ccgatgaaag cggcgacgcg cagttaatcc cacagccgcc agttccgctg gcggcatttt 
0841 aactttcttt aatgaagccg gaaaaatcct aaattcattt aatatttatc tttttaccgt ttcgctt