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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Proc Natl Acad Sci U S A. 1996;93(13):6464-9.
Complementation of an Escherichia coli adhE mutant by the Entamoeba histolytica EhADH2 gene provides a method for the identification of new antiamebic drugs.
Yong TS, Li E, Clark D, Stanley SL Jr.
(
8692838
)
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Deletion
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OCR68
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
adhE
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Related strains
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Strains Search
|
Search strains by gene name:
adhE
Search strains by all related name:
adhE adhC ana b1241 ECK1235 JW1228
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General information (Go to Linear View: )
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Gene Name
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adhE
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Alternative name
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adhC,ana,b1241,ECK1235,JW1228
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Location, Length
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1,294,669 - 1,297,344
(
-
)
;
27.90
min
;
2676
(bp) ,
891
(aa)
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Product
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fused acetaldehyde-CoAdehydrogenase/iron-dependent alcoholdehydrogenase/pyruvate-formate lyase deactivase
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Operon Name
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adhE
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Note
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CoA-linked acetaldehyde dehydrogenase and iron-dependent alcohol dehydrogenase; pyruvate-formate-lyase deactivase; GO_process: GO:0006113 - fermentation
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Function
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enzyme; Energy metabolism, carbon: Fermentation
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Gene Ontology
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GO:0003824
;
catalytic activity ( adhE )
GO:0004022
;
alcohol dehydrogenase activity ( adhE )
GO:0005506
;
iron ion binding ( adhE )
GO:0005515
;
protein binding ( adhE )
GO:0005624
;
membrane fraction ( adhE )
GO:0006066
;
alcohol metabolic process ( adhE )
GO:0008152
;
metabolic process ( adhE )
GO:0008774
;
acetaldehyde dehydrogenase (acetylating) activity ( adhE )
GO:0015976
;
carbon utilization ( adhE )
GO:0016491
;
oxidoreductase activity ( adhE )
GO:0046872
;
metal ion binding ( adhE )
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PID
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1787493
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EC number
(KEGG Pathway)
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1.1.1.1
1.2.1.10
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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adhE
(
EG10031
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
4
more]
)
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Entry name(Acc.no)
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ADHE_ECOLI
(
P17547
)
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- Protein name
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Aldehyde-alcohol dehydrogenase
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- Synonyms
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- Gene name
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Name=adhE; Synonyms=ana; OrderedLocusNames=b1241, z2016, ECs1741;
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MMBR References
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J Bacteriol. 1996;178:4327-4332
Translation of the adhE transcript to produce ethanol dehydrogenase requires RNase III cleavage in Escherichia coli.
Aristarkhov, A., A. Mikulskis, J. G. Belasco, and E. C. C. Lin.
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Gene. 1989;85(1):209-14.
Cloning and sequence analysis of the fermentative alcohol-dehydrogenase-encoding gene of Escherichia coli.
Goodlove PE, Cunningham PR, Parker J, Clark DP.
(
2695398
)
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Biochemistry. 1992;31(2):475-81.
Purification, characterization, and partial sequence of the glutathione-dependent formaldehyde dehydrogenase from Escherichia coli: a class III alcohol dehydrogenase.
Gutheil WG, Holmquist B, Vallee BL.
(
1731906
)
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FEBS Lett. 1991;281(1-2):59-63.
Pyruvate-formate-lyase-deactivase and acetyl-CoA reductase activities of Escherichia coli reside on a polymeric protein particle encoded by adhE.
Kessler D, Leibrecht I, Knappe J.
(
2015910
)
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J Bacteriol. 1991;173(5):1574-5.
Locations of genes in the nar-adhE region of the Escherichia coli K-12 chromosome.
Leonardo MR, Clark DP.
(
1999382
)
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J Bacteriol. 1993;175:870-878
Anaerobic regulation of the adhE gene, encoding the fermentative alcohol dehydrogenase of Escherichia coli.
Leonardo, M. R., P. R. Cunningham, and D. P. Clark.
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J Bacteriol. 1996;178:6013-6018
Role of NAD in regulating the adhE gene of Escherichia coli.
Leonardo, M. R., Y. Dailly, and D. P. Clark.
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J Bacteriol. 1982;152(2):935-8.
Escherichia coli mutants with a temperature-sensitive alcohol dehydrogenase.
Lorowitz W, Clark D.
(
6752127
)
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J Bacteriol. 1991;173(24):8009-13.
Regulation of the adhE gene, which encodes ethanol dehydrogenase in Escherichia coli.
Chen YM, Lin ECC.
(
1744060
)
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Mol Gen Genet. 1986;205(3):487-93.
The use of suicide substrates to select mutants of Escherichia coli lacking enzymes of alcohol fermentation.
Cunningham PR, Clark DP.
(
3550385
)
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| Sequences |
Amino acid
FASTA format
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0001 MAVTNVAELN ALVERVKKAQ REYASFTQEQ VDKIFRAAAL AAADARIPLA KMAVAESGMG IVEDKVIKNH
0071 FASEYIYNAY KDEKTCGVLS EDDTFGTITI AEPIGIICGI VPTTNPTSTA IFKSLISLKT RNAIIFSPHP
0141 RAKDATNKAA DIVLQAAIAA GAPKDLIGWI DQPSVELSNA LMHHPDINLI LATGGPGMVK AAYSSGKPAI
0211 GVGAGNTPVV IDETADIKRA VASVLMSKTF DNGVICASEQ SVVVVDSVYD AVRERFATHG GYLLQGKELK
0281 AVQDVILKNG ALNAAIVGQP AYKIAELAGF SVPENTKILI GEVTVVDESE PFAHEKLSPT LAMYRAKDFE
0351 DAVEKAEKLV AMGGIGHTSC LYTDQDNQPA RVSYFGQKMK TARILINTPA SQGGIGDLYN FKLAPSLTLG
0421 CGSWGGNSIS ENVGPKHLIN KKTVAKRAEN MLWHKLPKSI YFRRGSLPIA LDEVITDGHK RALIVTDRFL
0491 FNNGYADQIT SVLKAAGVET EVFFEVEADP TLSIVRKGAE LANSFKPDVI IALGGGSPMD AAKIMWVMYE
0561 HPETHFEELA LRFMDIRKRI YKFPKMGVKA KMIAVTTTSG TGSEVTPFAV VTDDATGQKY PLADYALTPD
0631 MAIVDANLVM DMPKSLCAFG GLDAVTHAME AYVSVLASEF SDGQALQALK LLKEYLPASY HEGSKNPVAR
0701 ERVHSAATIA GIAFANAFLG VCHSMAHKLG SQFHIPHGLA NALLICNVIR YNANDNPTKQ TAFSQYDRPQ
0771 ARRRYAEIAD HLGLSAPGDR TAAKIEKLLA WLETLKAELG IPKSIREAGV QEADFLANVD KLSEDAFDDQ
0841 CTGANPRYPL ISELKQILLD TYYGRDYVEG ETAAKKEAAP AKAEKKAKKS A
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ttgattttca taggttaagc aaatcatcac
-070 cgcactgact atactctcgt attcgagcag atgatttact aaaaaagttt aacattatca ggagagcatt
0001 atggctgtta ctaatgtcgc tgaacttaac gcactcgtag agcgtgtaaa aaaagcccag cgtgaatatg
0071 ccagtttcac tcaagagcaa gtagacaaaa tcttccgcgc cgccgctctg gctgctgcag atgctcgaat
0141 cccactcgcg aaaatggccg ttgccgaatc cggcatgggt atcgtcgaag ataaagtgat caaaaaccac
0211 tttgcttctg aatatatcta caacgcctat aaagatgaaa aaacctgtgg tgttctgtct gaagacgaca
0281 cttttggtac catcactatc gctgaaccaa tcggtattat ttgcggtatc gttccgacca ctaacccgac
0351 ttcaactgct atcttcaaat cgctgatcag tctgaagacc cgtaacgcca ttatcttctc cccgcacccg
0421 cgtgcaaaag atgccaccaa caaagcggct gatatcgttc tgcaggctgc tatcgctgcc ggtgctccga
0491 aagatctgat cggctggatc gatcaacctt ctgttgaact gtctaacgca ctgatgcacc acccagacat
0561 caacctgatc ctcgcgactg gtggtccggg catggttaaa gccgcataca gctccggtaa accagctatc
0631 ggtgtaggcg cgggcaacac tccagttgtt atcgatgaaa ctgctgatat caaacgtgca gttgcatctg
0701 tactgatgtc caaaaccttc gacaacggcg taatctgtgc ttctgaacag tctgttgttg ttgttgactc
0771 tgtttatgac gctgtacgtg aacgttttgc aacccacggc ggctatctgt tgcagggtaa agagctgaaa
0841 gctgttcagg atgttatcct gaaaaacggt gcgctgaacg cggctatcgt tggtcagcca gcctataaaa
0911 ttgctgaact ggcaggcttc tctgtaccag aaaacaccaa gattctgatc ggtgaagtga ccgttgttga
0981 tgaaagcgaa ccgttcgcac atgaaaaact gtccccgact ctggcaatgt accgcgctaa agatttcgaa
1051 gacgcggtag aaaaagcaga gaaactggtt gctatgggcg gtatcggtca tacctcttgc ctgtacactg
1121 accaggataa ccaaccggct cgcgtttctt acttcggtca gaaaatgaaa acggcgcgta tcctgattaa
1191 caccccagcg tctcagggtg gtatcggtga cctgtataac ttcaaactcg caccttccct gactctgggt
1261 tgtggttctt ggggtggtaa ctccatctct gaaaacgttg gtccgaaaca cctgatcaac aagaaaaccg
1331 ttgctaagcg agctgaaaac atgttgtggc acaaacttcc gaaatctatc tacttccgcc gtggctccct
1401 gccaatcgcg ctggatgaag tgattactga tggccacaaa cgtgcgctca tcgtgactga ccgcttcctg
1471 ttcaacaatg gttatgctga tcagatcact tccgtactga aagcagcagg cgttgaaact gaagtcttct
1541 tcgaagtaga agcggacccg accctgagca tcgttcgtaa aggtgcagaa ctggcaaact ccttcaaacc
1611 agacgtgatt atcgcgctgg gtggtggttc cccgatggac gccgcgaaga tcatgtgggt tatgtacgaa
1681 catccggaaa ctcacttcga agagctggcg ctgcgcttta tggatatccg taaacgtatc tacaagttcc
1751 cgaaaatggg cgtgaaagcg aaaatgatcg ctgtcaccac cacttctggt acaggttctg aagtcactcc
1821 gtttgcggtt gtaactgacg acgctactgg tcagaaatat ccgctggcag actatgcgct gactccggat
1891 atggcgattg tcgacgccaa cctggttatg gacatgccga agtccctgtg tgctttcggt ggtctggacg
1961 cagtaactca cgccatggaa gcttatgttt ctgtactggc atctgagttc tctgatggtc aggctctgca
2031 ggcactgaaa ctgctgaaag aatatctgcc agcgtcctac cacgaagggt ctaaaaatcc ggtagcgcgt
2101 gaacgtgttc acagtgcagc gactatcgcg ggtatcgcgt ttgcgaacgc cttcctgggt gtatgtcact
2171 caatggcgca caaactgggt tcccagttcc atattccgca cggtctggca aacgccctgc tgatttgtaa
2241 cgttattcgc tacaatgcga acgacaaccc gaccaagcag actgcattca gccagtatga ccgtccgcag
2311 gctcgccgtc gttatgctga aattgccgac cacttgggtc tgagcgcacc gggcgaccgt actgctgcta
2381 agatcgagaa actgctggca tggctggaaa cgctgaaagc tgaactgggt attccgaaat ctatccgtga
2451 agctggcgtt caggaagcag acttcctggc gaacgtggat aaactgtctg aagatgcatt cgatgaccag
2521 tgcaccggcg ctaacccgcg ttacccgctg atctccgagc tgaaacagat tctgctggat acctactacg
2591 gtcgtgatta tgtagaaggt gaaactgcag cgaagaaaga agctgctccg gctaaagctg agaaaaaagc
2661 gaaaaaatcc gcttaatcag tagcgctgtc tggcaacata aacggcccct tctgggcaat gccgatcagt
2731 taaggattag ttgaccgatc cttaaactga ggcactataa cggctt
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