MG1655
W3110
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Gene Report : hns
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Mol Gen Genet. 1989;218(2):361-3.
A locus involved in kanamycin, chloramphenicol and L-serine resistance is located in the bglY-galU region of the Escherichia coli K12 chromosome.
Lejeune P, Bertin P, Walon C, Willemot K, Colson C, Danchin A. ( 2506428 )
     Deletion OCR68 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  hns
Related strains
     Strains Search Search strains by gene name:  hns   Search strains by all related name:  hns B1 b1237 bglY cur drc drdX drs ECK1232 fimG H1 hnsA irk JW1225 msyA osmZ pilG topS topX verA virR

General information  (Go to Linear View:)
 Gene Name hns  
 Alternative name B1,b1237,bglY,cur,drc,drdX,drs,ECK1232,fimG,H1,hnsA,irk,JW1225,msyA,osmZ,pilG,topS,topX,verA,virR  
 Location, Length 1,291,732 - 1,292,145 (  -  ) ;   27.84 min ; 414 (bp) ,   137 (aa) Go to Linear View
 Product global DNA-binding transcriptional dual regulator H-NS  
 Operon Name hns  
 Note DNA-binding protein HLP-II (HU, BH2, HD, NS); pleiotropic regulator; GO_component: GO:0019861 - flagellum; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006350 - transcription; GO_process: GO:0006355 - regulation of transcription, DNA-dependent; GO_process: GO:0042330 - taxis  
 Function regulator; Basic proteins - synthesis, modification  
 Gene Ontology GO:0003677 ; DNA binding ( hns )
GO:0005622 ; intracellular ( hns )
GO:0006350 ; transcription ( hns )
GO:0006355 ; regulation of transcription, DNA-dependent ( hns )
GO:0007155 ; cell adhesion ( fimG )
GO:0009289 ; fimbrium ( fimG )
GO:0042995 ; cell projection ( fimG )
 PID 1787489  
 EC number
  (KEGG Pathway)
 
 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene hns ( EG10457 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) HNS_ECOLI ( P08936 )
    -  Protein name DNA-binding protein H-NS  
    -  Synonyms Histone-like protein HLP-II, Protein H1, Protein B1  
    -  Gene name OrderedLocusNames=b1237, c1701, z2013, ECs1739;  

 Linear View (Whole Mode)
View Location
   1280.0  –  1305.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP hns (  homologous genes of other bacterias  )   fimG (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain , H-Ns (Dna-Binding Domain)  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST DNA-binding protein H-NS (Pathogenesis protein kcpA)  
         PSI-BLAST DNA-binding protein H-NS (Pathogenesis protein kcpA)
    nr     (database updated : 2007.02.20 )
         BLAST DNA-binding protein HLP-II (HU, BH2, HD, NS); pleiotropic regulator [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    ATP_GTP_A     

Other Cross-Reference
    COG COG2916R 
    EcoCyc hns 

MMBR References
Mol Gen Genet. 1980;180(2):425-8.
cur-1, a mutation affecting a phenotype of sup+ strains of Escherichia coli.
Diderichsen B. ( 7007815 )
Mol Microbiol. 1996;19(5):965-75.
Antagonistic involvement of FIS and H-NS proteins in the transcriptional control of hns expression.
Falconi M, Brandi A, La Teana A, Gualerzi CO, Pon CL. ( 8830277 )
Mol Microbiol. 1995;16(1):1-7.
The regulation of transcription initiation by integration host factor.
Goosen N, van de Putte P. ( 7651128 )
Cell. 1988;52(4):569-84.
A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coli.
Higgins CF, Dorman CJ, Stirling DA, Waddell L, Booth IR, May G, Bremer E. ( 2830029 )
J Bacteriol. 1996;178:5790-5792
The nucleoid protein H-NS facilitates chromosome DNA replication in Escherichia coli dnaA mutants.
Katayama, T., M. Takata, and K. Sekimizu.
Proc Natl Acad Sci U S A. 1991;88:10907-10911
Identification of a cold shock transcriptional enhancer of the Escherichia coli gene encoding nucleoid protein H-NS.
La Teana, A., A. Brandi, M. Falconi, R. Spurio, C. L. Pon, and C. O. Gualerzi.
J Bacteriol. 1992;174:998-1006
Identification of two new genetically active regions associated with the osmZ locus of Escherichia coli: role in regulation of proU expression and mutagenic effect of cya, the structural gene for adenylate cyclase.
Barr, G. C., N. N. Bhriain, and C. J. Dorman.
Biochem Biophys Res Commun. 1984;119(3):1147-53.
A DNA-binding protein from E. coli isolation, characterization and its relationship with proteins H1 and B1.
Laine B, Sautiere P, Spassky A, Rimsky S. ( 6370250 )
Proc Natl Acad Sci U S A. 1990;87:360-363
Mutations in the bglY gene increase the frequency of spontaneous deletions in Escherichia coli K12.
Lejeune, P., and A. Danchin.
Mol Gen Genet. 1989;218(2):361-3.
A locus involved in kanamycin, chloramphenicol and L-serine resistance is located in the bglY-galU region of the Escherichia coli K12 chromosome.
Lejeune P, Bertin P, Walon C, Willemot K, Colson C, Danchin A. ( 2506428 )
J Bacteriol. 1991;173(5):1574-5.
Locations of genes in the nar-adhE region of the Escherichia coli K-12 chromosome.
Leonardo MR, Clark DP. ( 1999382 )
Mol Gen Genet. 1990;224(1):81-90.
The osmZ (bglY) gene encodes the DNA-binding protein H-NS (H1a), a component of the Escherichia coli K12 nucleoid.
May G, Dersch P, Haardt M, Middendorf A, Bremer E. ( 2177526 )
J Bacteriol. 1994;176:5537-5540
The H-NS protein is involved in the biogenesis of flagella in Escherichia coli.
Bertin, P., E. Terao, E. H. Lee, P. Lejeune, C. Colson, A. Danchin, and E. Collatz.
Mol Gen Genet. 1988;212(2):199-202.
Identification, cloning, nucleotide sequence and chromosomal map location of hns, the structural gene for Escherichia coli DNA-binding protein H-NS.
Pon CL, Calogero RA, Gualerzi CO. ( 2841565 )
J Bacteriol. 1996;178:4176-4181
Effects of H-NS and potassium glutamate on sigmaS- and sigma70-directed transcription in vitro from osmotically regulated P1 and P2 promoters of proU in Escherichia coli.
Rajkumari, K., S. Kusano, A. Ishihama, T. Mizuno, and J. Gowrishankar.
J Bacteriol. 1993;175:1182-1186
Modulation of acid-induced amino acid decarboxylase gene expression by hns in Escherichia coli.
Shi, X., B. C. Waasdorp, and G. N. Bennett.
J Bacteriol. 1986;168(1):179-85.
Metastable regulation of type 1 piliation in Escherichia coli and isolation and characterization of a phenotypically stable mutant.
Spears PA, Schauer D, Orndorff PE. ( 3019997 )
Mol Gen Genet. 1992;231(2):201-11.
Lethal overproduction of the Escherichia coli nucleoid protein H-NS: ultramicroscopic and molecular autopsy.
Spurio R, Durrenberger M, Falconi M, La Teana A, Pon CL, Gualerzi CO. ( 1310520 )
J Bacteriol. 1992;174:1454-1461
Multicopy suppression: an approach to understanding intracellular functioning of the protein export system.
Ueguchi, C., and K. Ito.
J Mol Biol. 1996;263(2):149-62.
Systematic mutational analysis revealing the functional domain organization of Escherichia coli nucleoid protein H-NS.
Ueguchi C, Suzuki T, Yoshida T, Tanaka K, Mizuno T. ( 8913298 )
Mol Gen Genet. 1991;230(1-2):332-6.
Molecular analysis of the Escherichia coli hns gene encoding a DNA-binding protein, which preferentially recognizes curved DNA sequences.
Yamada H, Yoshida T, Tanaka K, Sasakawa C, Mizuno T. ( 1745240 )
Mol Gen Genet. 1986;205(3):487-93.
The use of suicide substrates to select mutants of Escherichia coli lacking enzymes of alcohol fermentation.
Cunningham PR, Clark DP. ( 3550385 )

Sequences
Amino acid
FASTA format
0001 MSEALKILNN IRTLRAQARE CTLETLEEML EKLEVVVNER REEESAAAAE VEERTRKLQQ YREMLIADGI 
0071 DPNELLNSLA AVKSGTKAKR AQRPAKYSYV DENGETKTWT GQGRTPAVIK KAMDEQGKSL DDFLIKQ

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             caattttgaa ttccttacat tcctggctat 
-070 tgcacaactg aatttaaggc tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca 
0001 atgagcgaag cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg 
0071 aaacgctgga agaaatgctg gaaaaattag aagttgttgt taacgaacgt cgcgaagaag aaagcgcggc 
0141 tgctgctgaa gttgaagagc gcactcgtaa actgcagcaa tatcgcgaaa tgctgatcgc tgacggtatt 
0211 gacccgaacg aactgctgaa tagccttgct gccgttaaat ctggcaccaa agctaaacgt gctcagcgtc 
0281 cggcaaaata tagctacgtt gacgaaaacg gcgaaactaa aacctggact ggccaaggcc gtactccagc 
0351 tgtaatcaaa aaagcaatgg atgagcaagg taaatccctc gacgatttcc tgatcaagca ataatctttt 
0421 gtagattgca cttgcttaaa atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata 
0491 agaataagct ataaaaaaac ggcg