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PEC Original Annotations
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Essentiality
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|
Class
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non-essential
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|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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|
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Deletion
|
OCR68
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
purU
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Related strains
|
|
Strains Search
|
Search strains by gene name:
purU
Search strains by all related name:
purU b1232 ECK1227 JW1220 tgs ychI
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General information (Go to Linear View: )
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Gene Name
|
purU
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Alternative name
|
b1232,ECK1227,JW1220,tgs,ychI
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|
Location, Length
|
1,287,005 - 1,287,847
(
-
)
;
27.74
min
;
843
(bp) ,
280
(aa)
|
|
Product
|
formyltetrahydrofolate hydrolase
|
|
Operon Name
|
purU
|
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Note
|
formyltetrahydrofolate deformylase; for purT-dependent FGAR synthesis; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009152 - purine ribonucleotide biosynthetic process
|
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Function
|
enzyme; Purine ribonucleotide biosynthesis
|
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Gene Ontology
|
GO:0005515
;
protein binding ( purU )
GO:0006164
;
purine nucleotide biosynthetic process ( purU )
GO:0006189
;
'de novo' IMP biosynthetic process ( purU )
GO:0006730
;
one-carbon compound metabolic process ( purU )
GO:0008152
;
metabolic process ( purU )
GO:0008864
;
formyltetrahydrofolate deformylase activity ( purU )
GO:0009058
;
biosynthetic process ( purU )
GO:0016597
;
amino acid binding ( purU )
GO:0016742
;
hydroxymethyl-, formyl- and related transferase activity ( purU )
GO:0016787
;
hydrolase activity ( purU )
|
|
PID
|
1787483
|
EC number
(KEGG Pathway)
|
3.5.1.10
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
purU
(
EG11819
)
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|
Number of removed
|
0 aa cleaved
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SWISS-PROT
(
Show details
[
3
more]
)
|
Entry name(Acc.no)
|
PTGA_ECOLI
(
P08837
;
Q47703
)
|
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- Protein name
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PTS system, glucose-specific IIA component
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- Synonyms
|
EIIA-Glc, Glucose- permease IIA component, Phosphotransferase enzyme II, A component, EC 2.7.1.69, EIII-Glc
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- Gene name
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OrderedLocusNames=b2417, c2952, SF2472, S2618;
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Homology Analysis
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BLAST
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|
Bacteria
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|
GTOP
|
purU
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain B, Human Glycinamide Ribonucleotide Transformylase Domain At Ph 8.5
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
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BLAST
|
Formyltetrahydrofolate deformylase
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PSI-BLAST
|
Formyltetrahydrofolate deformylase
|
|
nr
(database updated :
2007.02.20
)
|
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BLAST
|
formyltetrahydrofolate deformylase [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
|
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PROSITE
|
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PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
AMIDATION
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MMBR References
|
J Bacteriol. 1995;177:1292-1298
Formyltetrahydrofolate hydrolase, a regulatory enzyme that functions to balance pools of tetrahydrofolate and one-carbon tetrahydrofolate adducts in Escherichia coli.
Nagy, P. L., A. Marolewski, S. J. Benkovic, and H. Zalkin.
|
J Bacteriol. 1993;175:7066-7073
purU, a source of formate for purT-dependent phosphoribosyl-N-formylglycinamide synthesis.
Nagy, P. L., G. M. McCorkle, and H. Zalkin.
|
J Biol Chem. 1997;272:15106-15112
Comparative enzymatic properties of GapB-encoded erythrose-4-phosphate dehydrogenase of Escherichia coli and phosphorylating glyceraldehyde-3-phosphate dehydrogenase.
Boschi-Muller, S., S. Azzi, D. Pollastro, C. Corbler, and G. Branlant.
|
Nucleic Acids Res. 1991;19:5863-5870
A novel RNA product of the tyrT operon of Escherichia coli.
Bosl, M., and H. Kersten.
|
J Bacteriol. 1994;176:221-231
Organization and functions of genes in the upstream region of tyrT of Escherichia coli: phenotypes of mutants with partial deletion of a new gene (tgs).
Bosl, M., and H. Kersten.
|
| Sequences |
Amino acid
FASTA format
|
0001 MHSLQRKVLR TICPDQKGLI ARITNICYKH ELNIVQNNEF VDHRTGRFFM RTELEGIFND STLLADLDSA
0071 LPEGSVRELN PAGRRRIVIL VTKEAHCLGD LLMKANYGGL DVEIAAVIGN HDTLRSLVER FDIPFELVSH
0141 EGLTRNEHDQ KMADAIDAYQ PDYVVLAKYM RVLTPEFVAR FPNKIINIHH SFLPAFIGAR PYHQAYERGV
0211 KIIGATAHYV NDNLDEGPII MQDVIHVDHT YTAEDMMRAG RDVEKNVLSR ALYKVLAQRV FVYGNRTIIL
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ccctgccctt gtggttcagg taaaaaattt
-070 aaaaagtgct gcggccaata atggttgacg gtacggttta gcaaacactc tcaacaaggt ttttccagca
0001 atgcattcac tccaacgtaa agttctgcgt actatttgtc cggaccaaaa aggtctgatc gcacgtatta
0071 ccaatatttg ctacaagcac gagttaaata tcgtacagaa caatgaattt gttgatcacc gtaccgggcg
0141 cttttttatg cgcacggaac tggaagggat ttttaatgat tccaccctgc tggcggatct cgatagcgca
0211 ttgccagaag gctccgtgcg tgagctgaat cctgccggtc gtcgccggat agtgattctg gtcactaaag
0281 aagcgcattg ccttggcgat ttgttgatga aagccaatta cggcggcctg gatgtcgaaa tcgcggcagt
0351 tattggtaac cacgatactt tacgttctct ggttgagcgt tttgatattc cgtttgagct ggtaagccat
0421 gaagggttaa cccgcaacga gcacgatcaa aagatggcgg atgccattga tgcttatcaa cctgactacg
0491 tggtgctggc gaagtatatg cgggtattaa cgccggaatt tgtggcacgc ttcccgaata agatcatcaa
0561 tattcaccat tcattcctgc cagcgtttat tggcgcacgt ccttatcacc aggcctatga acgtggtgtg
0631 aagattattg gcgcaaccgc tcactatgtg aatgacaatc tggacgaagg cccaatcatc atgcaggacg
0701 ttattcatgt cgatcatacc tacacagctg aagatatgat gcgcgcaggt cgtgacgtcg agaaaaacgt
0771 cttaagtcgt gcactataca aagtactggc acagcgcgtc tttgtttacg gtaatcgaac gattattctt
0841 taatcgccag caaaaataac tggttacctt taatccgtta cggatgaaaa ttacgcaacc agttcatttt
0911 tctcaacgta acactttaca gcggcgcgtc att
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