MG1655
W3110
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Gene Report : narJ
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Mol Microbiol. 1996;20(3):621-32.
Analysis of nitrate regulatory protein NarL-binding sites in the fdnG and narG operon control regions of Escherichia coli K-12.
Darwin AJ, Li J, Stewart V. ( 8736541 )
     Deletion OCR67 (D)  ,  OCR67-3 (D)  ,  OCR67-5 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  narJ
Related strains
     Strains Search Search strains by gene name:  narJ   Search strains by all related name:  narJ b1226 ECK1220 JW1217

General information  (Go to Linear View:)
 Gene Name narJ  
 Alternative name b1226,ECK1220,JW1217  
 Location, Length 1,284,362 - 1,285,072 (  +  ) ;   27.68 min ; 711 (bp) ,   236 (aa) Go to Linear View
 Product molybdenum-cofactor-assembly chaperone subunit (delta subunit) of nitrate reductase 1  
 Operon Name narGHJI  
 Note nitrate reductase 1, delta subunit, assembly function; GO_process: GO:0009061 - anaerobic respiration; GO_process: GO:0006457 - protein folding  
 Function enzyme; Energy metabolism, carbon: Anaerobic respiration  
 Gene Ontology GO:0005515; protein binding ( narJ )
GO:0006118; electron transport ( narJ )
GO:0008940; nitrate reductase activity ( narJ )
GO:0009325; nitrate reductase complex ( narJ )
GO:0016491; oxidoreductase activity ( narJ )
GO:0042128; nitrate assimilation ( narJ )
 PID 1787479  
 EC number
  (KEGG Pathway)
 
1.7.99.4  
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) NARJ_ECOLI ( P11351 )
    -  Protein name Respiratory nitrate reductase 1 delta chain  
    -  Synonyms EC 1.7.99.4  
    -  Gene name Name=narJ; OrderedLocusNames=b1226, z2003, ECs1731;  

 Linear View (Whole Mode)
View Location
   1275.0  –  1300.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP narJ (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
        PSI-BLAST  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Respiratory nitrate reductase 1 delta chain  
         PSI-BLAST Respiratory nitrate reductase 1 delta chain
    nr     (database updated : 2007.02.20 )
         BLAST nitrate reductase 1 delta subunit [Escherichia coli O157:H7 str. Sakai]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG2180C 
    EcoCyc narJ 

MMBR References
J Bacteriol. 1992;174:867-872
The narJ gene product is required for biogenesis of respiratory nitrate reductase in Escherichia coli.
Dubourdieu, M., and J. A. DeMoss.
J Bacteriol. 1991;173(5):1574-5.
Locations of genes in the nar-adhE region of the Escherichia coli K-12 chromosome.
Leonardo MR, Clark DP. ( 1999382 )
J Biol Chem. 1997;272:24266-24271
Characterization of NarJ, a system-specific chaperone required for nitrate reductase biogenesis in Escherichia coli.
Liu, X., and J. A. DeMoss.
Mol Microbiol. 1996;20(4):875-84.
Involvement of the narJ and mob gene products in distinct steps in the biosynthesis of the molybdoenzyme nitrate reductase in Escherichia coli.
Palmer T, Santini CL, Iobbi-Nivol C, Eaves DJ, Boxer DH, Giordano G. ( 8793883 )
Mol Gen Genet. 1989;218(2):249-56.
Nitrate reductase of Escherichia coli: completion of the nucleotide sequence of the nar operon and reassessment of the role of the alpha and beta subunits in iron binding and electron transfer.
Blasco F, Iobbi C, Giordano G, Chippaux M, Bonnefoy V. ( 2674654 )
J Bacteriol. 1988;170(4):1721-9.
narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes.
Sodergren EJ, DeMoss JA. ( 2832376 )

Sequences
Amino acid
FASTA format
0001 MIELVIVSRL LEYPDAALWQ HQQEMFEAIA ASKNLPKEDA HALGIFLRDL TTMDPLDAQA QYSELFDRGR 
0071 ATSLLLFEHV HGESRDRGQA MVDLLAQYEQ HGLQLNSREL PDHLPLYLEY LAQLPQSEAV EGLKDIAPIL 
0141 ALLSARLQQR ESRYAVLFDL LLKLANTAID SDKVAEKIAD EARDDTPQAL DAVWEEEQVK FFADKGCGDS 
0211 AITAHQRRFA GAVAPQYLNI TTGGQH

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             CCTTTGGTGA TGGCTGCCAC GGTTCAGATA 
-070 CCAAATTCAA TCTGTTCAAC AGCCGTCGTA TCGATGCCAT CGATGTGACC AGCAAAACGG AGCCGCATCC 
0001 atgatcgaac tcgtgattgt atcgcgtctc cttgaatatc cggatgctgc cttatggcag catcaacaag 
0071 agatgtttga ggcgattgcc gcgtcgaaaa atctgccaaa agaggatgcc catgcgctgg gcattttcct 
0141 gcgcgattta acgacgatgg atccgctcga tgcccaggcg cagtacagcg aactgttcga ccgtggccgc 
0211 gccacgtcac tgttgctgtt tgaacatgtg cacggcgaat cccgcgaccg cggccaggcg atggtggacc 
0281 tgctggcgca gtacgagcag cacggcttgc agttaaacag ccgcgaattg ccggaccatc tgccgctgta 
0351 tctggagtac ctggcgcagc tgccgcaaag cgaagccgtg gaaggtttga aagatatcgc gccgattctg 
0421 gcattgctga gcgcgcgtct gcaacagcgt gaaagccgtt atgccgtgct gtttgatctg ctgctgaaac 
0491 tggcgaatac cgctatcgac agcgacaaag tggcggaaaa aattgccgac gaagcgcgcg atgatacgcc 
0561 gcaggcgctg gatgctgtct gggaagaaga gcaggttaaa ttctttgctg acaaaggctg cggtgattca 
0631 gcaatcactg cgcatcagcg tcgctttgcc ggtgccgtcg cgccgcaata tctgaatatc accaccggag 
0701 gacagcacta aTGCAATTCC TGAATATGTT CTTCTTTGAT ATCTACCCGT ACATAGCCGG GGCGGTCTTC 
0771 CTGATTGGTA GCTGGCTGCG TTATGACTAC GGGCAGTACA C