|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Mol Microbiol. 1996;20(3):621-32.
Analysis of nitrate regulatory protein NarL-binding sites in the fdnG and narG operon control regions of Escherichia coli K-12.
Darwin AJ, Li J, Stewart V.
(
8736541
)
|
|
|
Deletion
|
OCR67
(D)
,
OCR67-3
(D)
,
OCR67-5
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
narH
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
narH
Search strains by all related name:
narH b1225 chlC ECK1219 JW1216
|
General information (Go to Linear View: )
|
|
Gene Name
|
narH
|
|
Alternative name
|
b1225,chlC,ECK1219,JW1216
|
|
Location, Length
|
1,282,827 - 1,284,365
(
+
)
;
27.65
min
;
1539
(bp) ,
512
(aa)
|
|
Product
|
nitrate reductase 1, beta (Fe-S) subunit
|
|
Operon Name
|
narGHJI
|
|
Note
|
nitrate reductase 1, beta subunit; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009061 - anaerobic respiration
|
|
Function
|
enzyme; Energy metabolism, carbon: Anaerobic respiration
|
|
Gene Ontology
|
GO:0005506
;
iron ion binding ( narH )
GO:0005515
;
protein binding ( narH )
GO:0005886
;
plasma membrane ( narH )
GO:0006118
;
electron transport ( narH )
GO:0006810
;
transport ( narH )
GO:0008940
;
nitrate reductase activity ( narH )
GO:0009055
;
electron carrier activity ( narH )
GO:0009325
;
nitrate reductase complex ( narH )
GO:0016020
;
membrane ( narH )
GO:0016491
;
oxidoreductase activity ( narH )
GO:0042126
;
nitrate metabolic process ( narH )
GO:0042128
;
nitrate assimilation ( narH )
GO:0046872
;
metal ion binding ( narH )
GO:0051536
;
iron-sulfur cluster binding ( narH )
GO:0051538
;
3 iron, 4 sulfur cluster binding ( narH )
GO:0051539
;
4 iron, 4 sulfur cluster binding ( narH )
|
|
PID
|
1787478
|
EC number
(KEGG Pathway)
|
1.7.99.4
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
narH
(
EG10639
)
|
|
Number of removed
|
0 aa cleaved
|
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
NARH_ECOLI
(
P11349
)
|
|
- Protein name
|
Respiratory nitrate reductase 1 beta chain
|
|
- Synonyms
|
EC 1.7.99.4, Nitrate reductase A beta subunit, Quinol-nitrate oxidoreductase beta subunit
|
|
- Gene name
|
Name=narH; OrderedLocusNames=b1225;
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Homology Analysis
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BLAST
|
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Bacteria
|
|
GTOP
|
narH
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain B, Crystal Structure Of Nitrate Reductase A, Narghi, From Escherichia Coli
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Respiratory nitrate reductase 1 beta chain
|
|
PSI-BLAST
|
Respiratory nitrate reductase 1 beta chain
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
nitrate reductase 1, beta (Fe-S) subunit [Escherichia coli K12]
|
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Pfam 28.0
(database updated :
2015-05
)
|
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Pfam
|
|
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PROSITE
|
|
PROSITE
|
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
CYTOCHROME_C
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MMBR References
|
J Bacteriol. 1983;153(3):1513-20.
chlC (nar) operon of Escherichia coli includes structural genes for alpha and beta subunits of nitrate reductase.
Edwards ES, Rondeau SS, DeMoss JA.
(
6298188
)
|
FEMS Microbiol Lett. 1981;12:125-129.
Characterization of the Escherichia coli chlC regulatory region in a cloned chlC-lac gene fusion.
Fimmel, A. L., and B. A. Haddock.
|
Eur J Biochem. 1997;250:567-577
Kinetics of membrane-bound nitrate reductase A from Escherichia coli with analogues of physiological electron donorsdifferent reaction sites for menadiol and duroquinol.
Giordani, R., J. Buc, A. Cornish-Bowden, and M. L. Cardenas.
|
FEMS Microbiol Lett. 1981;11:213-216.
Cloning the chlC gene for nitrate reductase of Escherichia coli.
Barr, G. C., and S. E. Palm-Nicholls.
|
J Bacteriol. 1991;173(5):1574-5.
Locations of genes in the nar-adhE region of the Escherichia coli K-12 chromosome.
Leonardo MR, Clark DP.
(
1999382
)
|
Mol Gen Genet. 1989;218(2):249-56.
Nitrate reductase of Escherichia coli: completion of the nucleotide sequence of the nar operon and reassessment of the role of the alpha and beta subunits in iron binding and electron transfer.
Blasco F, Iobbi C, Giordano G, Chippaux M, Bonnefoy V.
(
2674654
)
|
J Bacteriol. 1988;170(4):1721-9.
narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes.
Sodergren EJ, DeMoss JA.
(
2832376
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MKIRSQVGMV LNLDKCIGCH TCSVTCKNVW TSREGVEYAW FNNVETKPGQ GFPTDWENQE KYKGGWIRKI
0071 NGKLQPRMGN RAMLLGKIFA NPHLPGIDDY YEPFDFDYQN LHTAPEGSKS QPIARPRSLI TGERMAKIEK
0141 GPNWEDDLGG EFDKLAKDKN FDNIQKAMYS QFENTFMMYL PRLCEHCLNP ACVATCPSGA IYKREEDGIV
0211 LIDQDKCRGW RMCITGCPYK KIYFNWKSGK SEKCIFCYPR IEAGQPTVCS ETCVGRIRYL GVLLYDADAI
0281 ERAASTENEK DLYQRQLDVF LDPNDPKVIE QAIKDGIPLS VIEAAQQSPV YKMAMEWKLA LPLHPEYRTL
0351 PMVWYVPPLS PIQSAADAGE LGSNGILPDV ESLRIPVQYL ANLLTAGDTK PVLRALKRML AMRHYKRAET
0421 VDGKVDTRAL EEVGLTEAQA QEMYRYLAIA NYEDRFVVPS SHRELAREAF PEKNGCGFTF GDGCHGSDTK
0491 FNLFNSRRID AIDVTSKTEP HP
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CCGTAGGTTC TAACCGCGAT GAGTTTGTTG
-070 TAGTGCGTAA GATGAAGAAC ATTGACTGGT TAGATGGCGA AGGCAATGAC CAGGTACAGG AGAGCGTAAA
0001 atgaaaattc gttcacaagt cggcatggtg ctgaatctcg ataagtgcat cggctgccac acctgttcag
0071 ttacctgtaa aaacgtctgg accagccgtg aaggcgtgga atatgcgtgg ttcaacaacg tggaaaccaa
0141 gccgggccag ggcttcccga ctgactggga aaaccaggaa aaatacaaag gcggctggat ccgtaaaatc
0211 aacggcaaac tgcagccgcg catgggtaac cgtgccatgc tgctgggtaa aatcttcgct aacccgcatc
0281 tgccggggat cgacgattat tacgagccgt tcgattttga ctatcagaac ctgcataccg cgccggaagg
0351 cagcaaatcg cagccgattg cccgtccgcg ttcgctgatt accggggaac ggatggcgaa aatcgaaaaa
0421 gggccgaact gggaagatga cctgggcggt gagtttgaca aactggcgaa agacaagaac ttcgacaaca
0491 tccagaaggc gatgtatagc cagttcgaaa acaccttcat gatgtatttg ccgcgcctgt gcgaacactg
0561 cctgaacccg gcatgtgtgg cgacctgccc gagcggtgcg atttacaagc gtgaagaaga tggcatcgtc
0631 ctgatcgacc aggataaatg ccgtggctgg cgtatgtgca tcactggatg cccgtacaaa aaaatctact
0701 tcaactggaa gagcggtaag tctgagaagt gcatcttctg ctatccgcgt attgaagcgg gtcagccgac
0771 cgtgtgctca gaaacctgtg tcggtcgtat ccgttatctt ggcgtgctgt tgtacgatgc cgacgctatt
0841 gaacgtgcag ccagcaccga gaacgagaaa gatctttacc agcgtcagct ggacgtgttc ctcgatccga
0911 acgatccgaa agtcatcgag caggcgatta aagacggtat tccgctgagc gttattgaag ccgcacagca
0981 gtcgccggtt tataaaatgg caatggaatg gaaactggcg ctgccgctgc atccggaata tcgcacactg
1051 ccgatggtct ggtacgtgcc gcctctgtct ccgattcagt ctgcagcaga cgcgggtgag ctgggtagca
1121 acggcattct gccagacgtc gaaagcctgc gtattccggt acagtatctg gcgaatctgc tgaccgccgg
1191 tgataccaaa ccggtactgc gcgcactgaa acgtatgctg gcgatgcgtc attacaaacg tgctgaaacc
1261 gttgacggta aagttgatac ccgtgcgctg gaagaggtcg gtctgaccga agcccaggca caggagatgt
1331 accgttatct ggcgattgct aactacgaag atcgctttgt ggtgccgagt agtcatcgtg aactggcacg
1401 ggaagccttc ccggagaaaa atggctgcgg ctttaccttt ggtgatggct gccacggttc agataccaaa
1471 ttcaatctgt tcaacagccg tcgtatcgat gccatcgatg tgaccagcaa aacggagccg catccatgaT
1541 CGAACTCGTG ATTGTATCGC GTCTCCTTGA ATATCCGGAT GCTGCCTTAT GGCAGCATCA ACAAGAGATG
1611 TTTGAGGCGA TTGCCGCGTC GAAAAATCT
|
