MG1655
W3110
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Gene Report : narG
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Mol Microbiol. 1996;20(3):621-32.
Analysis of nitrate regulatory protein NarL-binding sites in the fdnG and narG operon control regions of Escherichia coli K-12.
Darwin AJ, Li J, Stewart V. ( 8736541 )
     Deletion OCR67 (D)  ,  OCR67-3 (D)  ,  OCR67-5 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  narG
Related strains
     Strains Search Search strains by gene name:  narG   Search strains by all related name:  narG b1224 chlC ECK1218 JW1215 narC

General information  (Go to Linear View:)
 Gene Name narG  
 Alternative name b1224,chlC,ECK1218,JW1215,narC  
 Location, Length 1,279,087 - 1,282,830 (  +  ) ;   27.57 min ; 3744 (bp) ,   1247 (aa) Go to Linear View
 Product nitrate reductase 1, alpha subunit  
 Operon Name narGHJI  
 Note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009061 - anaerobic respiration  
 Function enzyme; Energy metabolism, carbon: Anaerobic respiration  
 Gene Ontology GO:0005488; binding ( narG )
GO:0005506; iron ion binding ( narG )
GO:0005515; protein binding ( narG )
GO:0005886; plasma membrane ( narG )
GO:0006118; electron transport ( narG )
GO:0006810; transport ( narG )
GO:0008940; nitrate reductase activity ( narG )
GO:0009325; nitrate reductase complex ( narG )
GO:0016020; membrane ( narG )
GO:0016491; oxidoreductase activity ( narG )
GO:0030151; molybdenum ion binding ( narG )
GO:0042126; nitrate metabolic process ( narG )
GO:0042128; nitrate assimilation ( narG )
GO:0046872; metal ion binding ( narG )
GO:0051536; iron-sulfur cluster binding ( narG )
GO:0051539; 4 iron, 4 sulfur cluster binding ( narG )
 PID 1787477  
 EC number
  (KEGG Pathway)
 
1.7.99.4  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene narG ( EG10638 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) NARG_ECOLI ( P09152 ; P78294 )
    -  Protein name Respiratory nitrate reductase 1 alpha chain  
    -  Synonyms EC 1.7.99.4, Nitrate reductase A alpha subunit, Quinol-nitrate oxidoreductase alpha subunit  
    -  Gene name Name=narG; Synonyms=narC, bisD; OrderedLocusNames=b1224;  

 Linear View (Whole Mode)
View Location
   1270.0  –  1295.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP narG (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Nitrate Reductase A, Narghi, From Escherichia Coli  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Respiratory nitrate reductase 1 alpha chain  
         PSI-BLAST Respiratory nitrate reductase 1 alpha chain
    nr     (database updated : 2007.02.20 )
         BLAST nitrate reductase 1, alpha subunit [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    PROKAR_LIPOPROTEIN    LEUCINE_ZIPPER    MYB_1    MOLYBDOPTERIN_PROK_2    MOLYBDOPTERIN_PROK_1    MOLYBDOPTERIN_PROK_3     

Other Cross-Reference
    COG COG5013C 
    EcoCyc narG 

MMBR References
J Bacteriol. 1983;153(3):1513-20.
chlC (nar) operon of Escherichia coli includes structural genes for alpha and beta subunits of nitrate reductase.
Edwards ES, Rondeau SS, DeMoss JA. ( 6298188 )
J Bacteriol. 1983;154(2):719-27.
Membrane cytochromes of Escherichia coli chl mutants.
Hackett NR, Bragg PD. ( 6302081 )
FEMS Microbiol Lett. 1981;11:213-216.
Cloning the chlC gene for nitrate reductase of Escherichia coli.
Barr, G. C., and S. E. Palm-Nicholls.
J Bacteriol. 1991;173(5):1574-5.
Locations of genes in the nar-adhE region of the Escherichia coli K-12 chromosome.
Leonardo MR, Clark DP. ( 1999382 )
J Bacteriol. 1987;169(10):4614-20.
Promoter region of the nar operon of Escherichia coli: nucleotide sequence and transcription initiation signals.
Li SF, DeMoss JA. ( 3308846 )
J Bacteriol. 1985;164(1):25-32.
Delineation of two distinct regulatory domains in the 5' region of the nar operon of Escherichia coli.
Li S, Rabi T, DeMoss JA. ( 2995309 )
FEBS Lett. 1984;177(2):260-4.
Respiratory nitrate reductase of Escherichia coli. Sequence identification of the large subunit gene.
McPherson MJ, Baron AJ, Pappin DJ, Wootton JC. ( 6094247 )
J Bacteriol. 1984;159(1):159-66.
Construction in vitro of a cloned nar operon from Escherichia coli.
Rondeau SS, Hsu PY, DeMoss JA. ( 6330027 )
Mol Gen Genet. 1990;222(1):104-11.
Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon.
Blasco F, Iobbi C, Ratouchniak J, Bonnefoy V, Chippaux M. ( 2233673 )
J Bacteriol. 1988;170(4):1721-9.
narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes.
Sodergren EJ, DeMoss JA. ( 2832376 )

Sequences
Amino acid
FASTA format
0001 MSKFLDRFRY FKQKGETFAD GHGQLLNTNR DWEDGYRQRW QHDKIVRSTH GVNCTGSCSW KIYVKNGLVT 
0071 WETQQTDYPR TRPDLPNHEP RGCPRGASYS WYLYSANRLK YPMMRKRLMK MWREAKALHS DPVEAWASII 
0141 EDADKAKSFK QARGRGGFVR SSWQEVNELI AASNVYTIKN YGPDRVAGFS PIPAMSMVSY ASGARYLSLI 
0211 GGTCLSFYDW YCDLPPASPQ TWGEQTDVPE SADWYNSSYI IAWGSNVPQT RTPDAHFFTE VRYKGTKTVA 
0281 VTPDYAEIAK LCDLWLAPKQ GTDAAMALAM GHVMLREFHL DNPSQYFTDY VRRYTDMPML VMLEERDGYY 
0351 AAGRMLRAAD LVDALGQENN PEWKTVAFNT NGEMVAPNGS IGFRWGEKGK WNLEQRDGKT GEETELQLSL 
0421 LGSQDEIAEV GFPYFGGDGT EHFNKVELEN VLLHKLPVKR LQLADGSTAL VTTVYDLTLA NYGLERGLND 
0491 VNCATSYDDV KAYTPAWAEQ ITGVSRSQII RIAREFADNA DKTHGRSMII VGAGLNHWYH LDMNYRGLIN 
0561 MLIFCGCVGQ SGGGWAHYVG QEKLRPQTGW QPLAFALDWQ RPARHMNSTS YFYNHSSQWR YETVTAEELL 
0631 SPMADKSRYT GHLIDFNVRA ERMGWLPSAP QLGTNPLTIA GEAEKAGMNP VDYTVKSLKE GSIRFAAEQP 
0701 ENGKNHPRNL FIWRSNLLGS SGKGHEFMLK YLLGTEHGIQ GKDLGQQGGV KPEEVDWQDN GLEGKLDLVV 
0771 TLDFRLSSTC LYSDIILPTA TWYEKDDMNT SDMHPFIHPL SAAVDPAWEA KSDWEIYKAI AKKFSEVCVG 
0841 HLGKETDIVT LPIQHDSAAE LAQPLDVKDW KKGECDLIPG KTAPHIMVVE RDYPATYERF TSIGPLMEKI 
0911 GNGGKGIAWN TQSEMDLLRK LNYTKAEGPA KGQPMLNTAI DAAEMILTLA PETNGQVAVK AWAALSEFTG 
0981 RDHTHLALNK EDEKIRFRDI QAQPRKIISS PTWSGLEDEH VSYNAGYTNV HELIPWRTLS GRQQLYQDHQ 
1051 WMRDFGESLL VYRPPIDTRS VKEVIGQKSN GNQEKALNFL TPHQKWGIHS TYSDNLLMLT LGRGGPVVWL 
1121 SEADAKDLGI ADNDWIEVFN SNGALTARAV VSQRVPAGMT MMYHAQERIV NLPGSEITQQ RGGIHNSVTR 
1191 ITPKPTHMIG GYAHLAYGFN YYGTVGSNRD EFVVVRKMKN IDWLDGEGND QVQESVK

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             CGTTATCAAT TCCCACGCTG TTTCAGAGCG 
-070 TTACCTTGCC CTTAAACATT AGCAATGTCG ATTTATCAGA GGGCCGACAG GCTCCCACAG GAGAAAACCG 
0001 atgagtaaat tcctggaccg gtttcgctac ttcaaacaga agggtgaaac ctttgccgat gggcatggcc 
0071 agcttctcaa taccaaccgt gactgggagg atggatatcg ccagcgttgg cagcatgaca aaatcgtccg 
0141 ctctacccac ggggtaaact gcaccggctc ctgcagctgg aaaatctacg tcaaaaacgg tctggtcacc 
0211 tgggaaaccc agcagactga ctatccgcgt acccgtccgg atctgccaaa ccatgaacct cgcggctgcc 
0281 cgcgcggtgc cagctactcc tggtatcttt acagtgccaa ccgcctgaaa tacccgatga tgcgcaaacg 
0351 cctgatgaaa atgtggcgtg aagcgaaggc gctgcatagc gatccggttg aggcatgggc ttctatcatt 
0421 gaagacgccg ataaagcgaa aagctttaag caggcgcgtg gacgcggtgg atttgttcgt tcttcctggc 
0491 aggaggtgaa cgaactgatc gccgcatcta acgtttacac catcaaaaac tacggcccgg accgtgttgc 
0561 tggtttctcg ccaattccgg caatgtcgat ggtttcttac gcatcgggtg cacgctatct ctcgctgatt 
0631 ggcggtactt gcttaagctt ctacgactgg tactgcgact tgcctcctgc gtctccgcaa acctggggcg 
0701 agcaaactga cgtaccggaa tctgctgact ggtacaactc cagctacatc atcgcctggg ggtcaaacgt 
0771 gccgcagacg cgtaccccgg atgctcactt ctttactgaa gtgcgttaca aagggaccaa aactgttgcc 
0841 gtcacaccag actacgctga aatcgccaaa ctgtgcgatc tgtggctggc accgaaacag ggcaccgatg 
0911 cggcaatggc gctggcgatg ggccacgtaa tgctgcgtga attccacctc gacaacccaa gccagtattt 
0981 caccgactat gtgcgtcgct acaccgacat gccgatgctg gtgatgctgg aagaacgcga cggttactac 
1051 gctgcaggtc gtatgctgcg cgctgctgat ctggttgatg cgctgggcca ggaaaacaat ccggaatgga 
1121 aaactgtcgc ctttaatacc aatggcgaaa tggttgcgcc gaacggttct attggcttcc gctggggcga 
1191 gaagggcaaa tggaatcttg agcagcgcga cggcaaaact ggcgaagaaa ccgagctgca actgagcctg 
1261 ctgggtagcc aggatgagat cgctgaggta ggcttcccgt actttggtgg cgacggcacg gaacacttca 
1331 acaaagtgga actggaaaac gtgctgctgc acaaactgcc ggtgaaacgc ctgcaactgg ctgatggcag 
1401 caccgccctg gtgaccaccg tttatgatct gacgctggca aactacggtc tggaacgtgg cctgaacgac 
1471 gttaactgtg caaccagcta tgacgatgtg aaagcttata ccccggcctg ggccgagcag attaccggcg 
1541 tttctcgcag ccagattatt cgcatcgccc gtgaatttgc cgataacgct gataaaacgc acggtcgttc 
1611 gatgattatc gtcggtgcgg ggctgaacca ctggtatcac ctcgatatga actatcgtgg tctgatcaac 
1681 atgctgattt tctgcggctg tgtcggtcag agcgggggcg gctgggcgca ctatgtaggt caggaaaaac 
1751 tgcgtccgca aaccggctgg cagccgctgg cgtttgccct tgactggcag cgtccggcgc gtcacatgaa 
1821 cagcacttct tatttctata accactccag ccagtggcgt tatgaaaccg tcacggcgga agagttgctg 
1891 tcaccgatgg cggacaaatc ccgctatacc ggacacttga tcgactttaa cgtccgtgcg gaacgcatgg 
1961 gctggctgcc gtctgcaccg cagttaggca ctaacccgct gactatcgct ggcgaagcgg aaaaagccgg 
2031 gatgaatccg gtggactata cggtgaaatc cctgaaagag ggttccatcc gttttgcggc agaacaacca 
2101 gaaaacggta aaaaccaccc gcgcaacctg ttcatctggc gttctaacct gctcggttct tccggtaaag 
2171 gtcatgagtt tatgctcaag tacctgctgg ggacggagca cggtatccag ggtaaagatc tggggcaaca 
2241 gggcggcgtg aagccggaag aagtggactg gcaggacaat ggtctggaag gcaagctgga tctggtggtt 
2311 acgctggact tccgtctgtc gagcacctgt ctctattccg acatcatttt gccgacggcg acctggtacg 
2381 aaaaagacga catgaatact tcggatatgc atccgtttat tcacccgctg tctgcggcgg tcgatccggc 
2451 ctgggaagcg aaaagcgact gggaaatcta caaagccatc gcgaagaaat tctccgaagt gtgcgtcggc 
2521 catctgggta aagaaaccga catcgtcacg ctgcctatcc agcatgactc tgccgctgaa ctggcgcagc 
2591 cgctggatgt gaaagactgg aaaaaaggcg agtgcgacct gatcccaggt aaaaccgcgc cacacattat 
2661 ggtcgtagag cgcgattatc cggcgactta cgaacgcttt acctctatcg gcccgctgat ggagaaaatc 
2731 ggtaatggcg gtaaagggat tgcctggaac acccagagcg agatggatct gctgcgtaag ctcaactaca 
2801 ccaaagcgga aggtccggcg aaaggccagc cgatgctgaa caccgcaatt gatgcggcag agatgatcct 
2871 gacactggca ccggaaacca acggtcaggt agccgtgaaa gcctgggctg ccctgagcga atttaccggt 
2941 cgtgaccata cgcatctggc gctgaataaa gaagacgaga agatccgctt ccgcgatatt caggcacagc 
3011 cgcgcaaaat tatctccagc ccgacctggt ctggtctgga agatgaacac gtttcttaca acgccggtta 
3081 caccaacgtt cacgagctga tcccatggcg tacgctctct ggtcgtcagc aactgtatca ggatcaccag 
3151 tggatgcgtg atttcggtga aagcctgctg gtttatcgtc cgccgatcga cacccgttcg gtgaaagaag 
3221 tgataggcca gaaatccaac ggcaaccagg aaaaagcgct caacttcctg acgccgcacc agaagtgggg 
3291 tatccactcc acctacagcg acaacctgct gatgctgact ttaggtcgcg gtggtccggt ggtctggttg 
3361 agtgaagccg atgccaaaga tctgggtatc gccgataacg actggattga agtcttcaac agcaacggtg 
3431 ctctgactgc ccgtgcggtt gtcagccagc gtgttccggc agggatgacc atgatgtacc acgcgcagga 
3501 acgtatcgtt aacctgcctg gttcggaaat tacccaacag cgtggtggta tccataactc ggtcacccgt 
3571 atcacgccga aaccgacgca tatgatcggc ggctatgccc atctggcata cggctttaac tactatggca 
3641 ccgtaggttc taaccgcgat gagtttgttg tagtgcgtaa gatgaagaac attgactggt tagatggcga 
3711 aggcaatgac caggtacagg agagcgtaaa atgaAAATTC GTTCACAAGT CGGCATGGTG CTGAATCTCG 
3781 ATAAGTGCAT CGGCTGCCAC ACCTGTTCAG TTACCTGTAA AAACGTCTGG ACCAGCCGTG AAGG