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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
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Deletion
|
OCR67
(D)
,
OCR67-5
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
narK
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Related strains
|
|
Strains Search
|
Search strains by gene name:
narK
Search strains by all related name:
narK b1223 ECK1217 JW1214
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General information (Go to Linear View: )
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Gene Name
|
narK
|
|
Alternative name
|
b1223,ECK1217,JW1214
|
|
Location, Length
|
1,277,180 - 1,278,571
(
+
)
;
27.53
min
;
1392
(bp) ,
463
(aa)
|
|
Product
|
nitrate/nitrite transporter
|
|
Operon Name
|
narK
|
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Note
|
nitrite extrusion protein; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0006807 - nitrogen compound metabolic process
|
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Function
|
transport; Transport of small molecules: Anions
|
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Gene Ontology
|
GO:0005886
;
plasma membrane ( narK )
GO:0006810
;
transport ( narK )
GO:0015103
;
inorganic anion transmembrane transporter activity ( narK )
GO:0015698
;
inorganic anion transport ( narK )
GO:0016020
;
membrane ( narK )
GO:0016021
;
integral to membrane ( narK )
GO:0042128
;
nitrate assimilation ( narK )
|
|
PID
|
1787475
|
EC number
(KEGG Pathway)
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|
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
NARK_ECOLI
(
P10903
)
|
|
- Protein name
|
Nitrite extrusion protein 1
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- Synonyms
|
Nitrite facilitator 1
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|
- Gene name
|
Name=narK; OrderedLocusNames=b1223;
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Homology Analysis
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BLAST
|
|
Bacteria
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GTOP
|
narK
(
homologous genes of other bacterias
)
|
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PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
|
|
SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Nitrite extrusion protein 1
|
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PSI-BLAST
|
High-affinity glucose transporter SNF3
|
|
nr
(database updated :
2007.02.20
)
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BLAST
|
nitrite extrusion protein [Escherichia coli UTI89]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
|
|
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PROSITE
|
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PROSITE
|
ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
AMIDATION
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MMBR References
|
J Bacteriol. 1991;173(11):3303-10.
NarK enhances nitrate uptake and nitrite excretion in Escherichia coli.
DeMoss JA, Hsu PY.
(
2045360
)
|
J Bacteriol. 1991;173(5):1574-5.
Locations of genes in the nar-adhE region of the Escherichia coli K-12 chromosome.
Leonardo MR, Clark DP.
(
1999382
)
|
Mol Gen Genet. 1990;222(1):104-11.
Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon.
Blasco F, Iobbi C, Ratouchniak J, Bonnefoy V, Chippaux M.
(
2233673
)
|
J Bacteriol. 1988;170(4):1589-97.
Identification and expression of genes narL and narX of the nar (nitrate reductase) locus in Escherichia coli K-12.
Stewart V, Parales J Jr.
(
2832370
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSHSSAPERA TGAVITDWRP EDPAFWQQRG QRIASRNLWI SVPCLLLAFC VWMLFSAVAV NLPKVGFNFT
0071 TDQLFMLTAL PSVSGALLRV PYSFMVPIFG GRRWTAFSTG ILIIPCVWLG FAVQDTSTPY SVFIIISLLC
0141 GFAGANFASS MANISFFFPK QKQGGALGLN GGLGNMGVSV MQLVAPLVVS LSIFAVFGSQ GVKQPDGTEL
0211 YLANASWIWV PFLAIFTIAA WFGMNDLATS KASIKEQLPV LKRGHLWIMS LLYLATFGSF IGFSAGFAML
0281 SKTQFPDVQI LQYAFFGPFI GALARSAGGA LSDRLGGTRV TLVNFILMAI FSGLLFLTLP TDGQGGSFMA
0351 FFAVFLALFL TAGLGSGSTF QMISVIFRKL TMDRVKAEGG SDERAMREAA TDTAAALGFI SAIGAIGGFF
0421 IPKAFGSSLA LTGSPVGAMK VFLIFYIACV VITWAVYGRH SKK
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CAATGATAGA TAAAGTTATC TTATCGTTTG
-070 ATTTACATCA AATTGCCTTT AGCTACAGAC ACTAAGGTGG CAGACATCGA AACGAGTATC AGAGGTGTCT
0001 atgagtcact catccgcccc cgaaagggct actggagctg tcattacaga ttggcgaccg gaagatcctg
0071 cgttctggca acaacgcggt caacgtattg ccagccgcaa cctgtggatt tccgttccct gtctgctgct
0141 ggcgttttgc gtatggatgt tgttcagcgc tgttgcggtg aacctaccga aagtcggctt taattttacg
0211 accgatcagc tatttatgtt gactgcgctg ccttcggttt ctggcgcgtt attacgtgtt ccatactcct
0281 ttatggttcc tatcttcggt ggtcgtcgct ggacggcgtt cagcaccggt attctgatta ttccttgcgt
0351 ctggctgggt tttgccgtgc aggatacctc cacgccttat agcgtcttca tcatcatctc tctgctatgc
0421 ggctttgctg gcgcgaactt cgcatccagt atggcaaaca tcagcttctt ctttccgaaa cagaagcagg
0491 gtggcgcgct gggtctgaat ggtggtctgg gaaacatggg cgtcagcgtc atgcagttgg ttgctccgct
0561 ggtggtatca ctgtcgattt tcgcagtatt tggtagccag ggcgtcaaac agccggatgg gactgagctg
0631 tatctggcga atgcgtcctg gatatgggtg ccgttccttg ccatcttcac cattgcggcg tggtttggca
0701 tgaacgatct tgctacctcg aaagcctcca tcaaggagca gttgccggta ctcaaacggg gtcatctgtg
0771 gattatgagc ctgctgtatc tggcaacctt cggctccttc atcggcttct ccgcgggctt tgcaatgctg
0841 tcaaaaacgc agttcccgga tgttcagatt ctgcaatacg ctttcttcgg gccgtttatt ggtgcgctgg
0911 cgcgttctgc aggtggtgca ttatctgacc gtctgggcgg aactcgtgtc acgctggtga actttattct
0981 gatggcgatt ttcagcggcc tgctgttcct gaccttaccg actgacgggc agggcggaag cttcatggcg
1051 ttcttcgcgg tcttcctggc gctgttcctg acagctgggc tgggtagtgg ttccactttc cagatgattt
1121 cagtgatctt ccgtaaactg acaatggatc gcgtgaaagc agaagggggt tctgacgaac gtgcgatgcg
1191 tgaagcggca accgacacgg cggcggcgct gggtttcatc tctgcgattg gcgcgattgg tggcttcttt
1261 atcccgaaag cgtttggtag ctcgctggca ttaacgggtt cgccagtcgg cgcaatgaag gtatttttga
1331 ttttctatat cgcctgcgta gtgattacct gggcggtata tggtcggcat tctaaaaaat aaACCGTTAC
1401 TCGTCATACT TCGGGTTACA TGTGCTGCGG CTGCGTTCAT TCACCCCAGT CACTTACTTT AGTAAGCTCC
1471 TGGGATTCAT TCACTTGCCG CC
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