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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Biol Chem. 1995;270(23):13698-705.
Overproduction and one-step purification of Escherichia coli 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase and oxygen transfer studies during catalysis using isotopic-shifted heteronuclear NMR.
Dotson GD, Dua RK, Clemens JC, Wooten EW, Woodard RW.
(
7775423
)
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Deletion
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OCR67
(D)
,
OCR67-5
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
kdsA
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Related strains
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Strains Search
|
Search strains by gene name:
kdsA
Search strains by all related name:
kdsA b1215 ECK1203 JW1206
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General information (Go to Linear View: )
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Gene Name
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kdsA
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Alternative name
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b1215,ECK1203,JW1206
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Location, Length
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1,267,388 - 1,268,242
(
+
)
;
27.32
min
;
855
(bp) ,
284
(aa)
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Product
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3-deoxy-D-manno-octulosonate 8-phosphate synthase
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Operon Name
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ychQA-kdsA
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Note
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2-dehydro-3-deoxyphosphooctulonate aldolase; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0042280 - cell surface antigen activity, host-interacting; GO_process: GO:0009244 - lipopolysaccharide core region biosynthetic process; GO_process: GO:0009248 - K antigen biosynthetic process
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Function
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enzyme; Surface polysaccharides & antigens
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Gene Ontology
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GO:0003824
;
catalytic activity ( kdsA )
GO:0005737
;
cytoplasm ( kdsA )
GO:0008152
;
metabolic process ( kdsA )
GO:0008676
;
3-deoxy-8-phosphooctulonate synthase activity ( kdsA )
GO:0009058
;
biosynthetic process ( kdsA )
GO:0009103
;
lipopolysaccharide biosynthetic process ( kdsA )
GO:0016740
;
transferase activity ( kdsA )
GO:0019294
;
ketodeoxyoctanoate biosynthetic process ( kdsA )
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PID
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1787466
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EC number
(KEGG Pathway)
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2.5.1.55
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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kdsA
(
EG10518
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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KDSA_ECOLI
(
P17579
)
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- Protein name
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2-dehydro-3-deoxyphosphooctonate aldolase
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- Synonyms
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EC 2.5.1.55, Phospho-2- dehydro-3-deoxyoctonate aldolase, 3-deoxy-D-manno-octulosonic acid 8- phosphate synthetase, KDO-8-phosphate synthetase, KDO 8-P synthase, KDOPS
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- Gene name
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Name=kdsA; OrderedLocusNames=b1215, SF1218, S1302;
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MMBR References
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J Bacteriol. 1988;170(11):5382-4.
Localization of the kdsA gene with the aid of the physical map of the Escherichia coli chromosome.
Woisetschlager M, Hodl-Neuhofer A, Hogenauer G.
(
3053664
)
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Mol Gen Genet. 1987;207(2-3):369-73.
The kdsA gene coding for 3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase is part of an operon in Escherichia coli.
Woisetschlager M, Hogenauer G.
(
3039295
)
|
| Sequences |
Amino acid
FASTA format
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0001 MKQKVVSIGD INVANDLPFV LFGGMNVLES RDLAMRICEH YVTVTQKLGI PYVFKASFDK ANRSSIHSYR
0071 GPGLEEGMKI FQELKQTFGV KIITDVHEPS QAQPVADVVD VIQLPAFLAR QTDLVEAMAK TGAVINVKKP
0141 QFVSPGQMGN IVDKFKEGGN EKVILCDRGA NFGYDNLVVD MLGFSIMKKV SGNSPVIFDV THALQCRDPF
0211 GAASGGRRAQ VAELARAGMA VGLAGLFIEA HPDPEHAKCD GPSALPLAKL EPFLKQMKAI DDLVKGFEEL
0281 DTSK
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CGATCAGCGA AATGATCCGT GCGCAAATAA
-070 ATAACATCGC GCATAAACAT ATTGTGCTGC ATTAATTAAT CGACATTTTA CTCAAGATTA AGGCGATCCT
0001 atgaaacaaa aagtggttag cattggcgac atcaacgtag caaatgacct gccgttcgta ctgtttggcg
0071 gtatgaacgt gttggaatct cgcgatctgg cgatgcgcat ttgcgagcac tacgtaactg tgacccagaa
0141 actgggtatc ccttacgtgt tcaaagcctc ttttgacaaa gccaaccgct cctccatcca ctcttatcgt
0211 ggaccgggcc tggaagaagg gatgaaaatc ttccaggagt tgaagcagac ttttggcgtg aaaattatca
0281 ccgacgttca cgaaccaagt caggcacagc ccgttgctga tgtcgtggat gtgattcagt tgccggcgtt
0351 tcttgctcgc cagactgacc tggttgaagc catggcgaaa accggtgcgg taattaacgt caagaaacca
0421 cagtttgtca gcccgggaca gatgggtaat atcgttgata aattcaaaga aggcggcaac gaaaaagtga
0491 ttctttgcga tcgcggtgct aacttcggct atgacaacct ggttgtcgat atgctgggct tcagcattat
0561 gaagaaagtg tctggtaact cgccggtgat tttcgacgtg acccacgcac tgcaatgccg cgatccgttt
0631 ggcgcagctt ccggtggtcg tcgtgctcag gtggctgagc tggcacgagc cggtatggcg gtaggtctgg
0701 cggggctgtt tattgaagcg catccggatc cggaacatgc gaaatgtgat ggtccatccg cgctgccgct
0771 ggctaaactg gaaccgttcc tcaagcagat gaaagcgatt gatgatctgg tgaaaggttt cgaagaactg
0841 gataccagca agtaaTCTTT TTTGCTTGAA AAATAAAGTA TTAGCGTTCT GCGTTAAGAC TTTTTTCATG
0911 GGTGCCGGAT ACAAAAAAGG CCGCAGGCTG TTACCCCTGC GGCCG
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