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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Genes Genet Syst. 1996;71(4):237-41.
Control of the availability of exogenous 5-aminolevulinic acid in Escherichia coli.
Nakayashiki T, Inokuchi H.
(
8979375
)
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Deletion
|
OCR67
(D)
,
OCR67-5
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
hemA
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Related strains
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Strains Search
|
Search strains by gene name:
hemA
Search strains by all related name:
hemA b1210 ECK1198 gtrA JW1201
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General information (Go to Linear View: )
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Gene Name
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hemA
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Alternative name
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b1210,ECK1198,gtrA,JW1201
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Location, Length
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1,262,937 - 1,264,193
(
+
)
;
27.22
min
;
1257
(bp) ,
418
(aa)
|
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Product
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glutamyl tRNA reductase
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Operon Name
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hemA-prfA-prmC
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Note
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glutamyl-tRNA reductase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0042493 - response to drug
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Function
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enzyme; Biosynthesis of cofactors, carriers: Heme, porphyrin
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Gene Ontology
|
GO:0003824
;
catalytic activity ( hemA )
GO:0004764
;
shikimate 5-dehydrogenase activity ( hemA )
GO:0005488
;
binding ( hemA )
GO:0005737
;
cytoplasm ( hemA )
GO:0006779
;
porphyrin biosynthetic process ( hemA )
GO:0008152
;
metabolic process ( hemA )
GO:0008883
;
glutamyl-tRNA reductase activity ( hemA )
GO:0016491
;
oxidoreductase activity ( hemA )
GO:0033014
;
tetrapyrrole biosynthetic process ( hemA )
GO:0050661
;
NADP binding ( hemA )
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PID
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1787461
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EC number
(KEGG Pathway)
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1.2.1.-
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
|
HEM1_ECOLI
(
P13580
;
Q59405
)
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- Protein name
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Glutamyl-tRNA reductase
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- Synonyms
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EC 1.2.1.-, GluTR
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- Gene name
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Name=hemA; OrderedLocusNames=b1210, z1981, ECs1715;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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hemA
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, Oxoacyl-Acp Reductase Of Plasmodium Falciparum
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Glutamyl-tRNA reductase (GluTR)
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PSI-BLAST
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Glutamyl-tRNA reductase
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nr
(database updated :
2007.02.20
)
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BLAST
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glutamyl-tRNA reductase [Escherichia coli O157:H7 str. Sakai]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
PROKAR_LIPOPROTEIN
GLUTR
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MMBR References
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Mol Gen Genet. 1989;216(2-3):347-52.
Isolation and nucleotide sequence of the hemA gene of Escherichia coli K12.
Drolet M, Peloquin L, Echelard Y, Cousineau L, Sasarman A.
(
2664455
)
|
J Bacteriol. 1989;171(6):2919-24.
Identification of the enzymatic basis for delta-aminolevulinic acid auxotrophy in a hemA mutant of Escherichia coli.
Avissar YJ, Beale SI.
(
2656630
)
|
Gene. 1992;121(1):127-32.
Cloning and characterization of genes involved in the biosynthesis of delta-aminolevulinic acid in Escherichia coli.
Ikemi M, Murakami K, Hashimoto M, Murooka Y.
(
1427085
)
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Gene. 1989;82(2):209-17.
Cloning and structure of the hem A gene of Escherichia coli K-12.
Li JM, Russell CS, Cosloy SD.
(
2684779
)
|
J Bacteriol. 1997;179:4583-4590
Role of the hemA gene product and delta-aminolevulinic acid in regulation of Escherichia coli heme synthesis.
Verderber, E., L. J. Lucast, J. A. Van Dehy, P. Cozart, J. B. Etter, and E. A. Best.
|
J Bacteriol. 1989;171(9):4728-35.
Isolation, nucleotide sequence, and preliminary characterization of the Escherichia coli K-12 hemA gene.
Verkamp E, Chelm BK.
(
2548996
)
|
J Bacteriol. 1994;176:2743-2746
5-Aminolevulinic acid synthesis in Escherichia coli requires expression of hemA.
Chen, W., C. S. Russell, Y. Murooka, and S. D. Cosloy.
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| Sequences |
Amino acid
FASTA format
|
0001 MTLLALGINH KTAPVSLRER VSFSPDKLDQ ALDSLLAQPM VQGGVVLSTC NRTELYLSVE EQDNLQEALI
0071 RWLCDYHNLN EEDLRKSLYW HQDNDAVSHL MRVASGLDSL VLGEPQILGQ VKKAFADSQK GHMKASELER
0141 MFQKSFSVAK RVRTETDIGA SAVSVAFAAC TLARQIFESL STVTVLLVGA GETIELVARH LREHKVQKMI
0211 IANRTRERAQ ILADEVGAEV IALSDIDERL READIIISST ASPLPIIGKG MVERALKSRR NQPMLLVDIA
0281 VPRDVEPEVG KLANAYLYSV DDLQSIISHN LAQRKAAAVE AETIVAQETS EFMAWLRAQS ASETIREYRS
0351 QAEQVRDELT AKALAALEQG GDAQAIMQDL AWKLTNRLIH APTKSLQQAA RDGDNERLNI LRDSLGLE
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CAATTACTCC AAAAGGGGGC GCTCTCTTTT
-070 ATTGATCTTA CGCATCCTGT ATGATGCAAG CAGACTAACC CTATCAACGT TGGTATTATT TCCCGCAGAC
0001 atgacccttt tagcactcgg tatcaaccat aaaacggcac ctgtatcgct gcgagaacgt gtatcgtttt
0071 cgccggataa gctcgatcag gcgcttgaca gcctgcttgc gcagccgatg gtgcagggcg gcgtggtgct
0141 gtcgacgtgc aaccgcacgg aactttatct tagcgttgaa gagcaggaca acctgcaaga ggcgttaatc
0211 cgctggcttt gcgattatca caatcttaat gaagaagatc tgcgtaaaag cctctactgg catcaggata
0281 acgacgcggt tagccattta atgcgtgttg ccagcggcct ggattcactg gttctggggg agccgcagat
0351 cctcggtcag gttaaaaaag cgtttgccga ttcgcaaaaa ggtcatatga aggccagcga actggaacgc
0421 atgttccaga aatctttctc tgtcgcgaaa cgcgttcgca ctgaaacaga tatcggtgcc agcgctgtgt
0491 ctgtcgcttt tgcggcttgt acgctggcgc ggcagatctt tgaatcgctc tctacggtca cagtgttgct
0561 ggtaggcgcg ggcgaaacta tcgagctggt ggcgcgtcat ctgcgcgaac acaaagtaca gaagatgatt
0631 atcgccaacc gcactcgcga acgtgcccaa attctggcag atgaagtcgg cgcggaagtg attgccctga
0701 gtgatatcga cgaacgtctg cgcgaagccg atatcatcat cagttccacc gccagcccgt taccgattat
0771 cgggaaaggc atggtggagc gcgcattaaa aagccgtcgc aaccaaccaa tgctgttggt ggatattgcc
0841 gttccgcgcg atgttgagcc ggaagttggc aaactggcga atgcttatct ttatagcgtt gatgatctgc
0911 aaagcatcat ttcgcacaac ctggcgcagc gtaaagccgc agcggttgag gcggaaacta ttgtcgctca
0981 ggaaaccagc gaatttatgg cgtggctgcg agcacaaagc gccagcgaaa ccattcgcga gtatcgcagc
1051 caggcagagc aagttcgcga tgagttaacc gccaaagcgt tagcggccct tgagcagggc ggcgacgcgc
1121 aagccattat gcaggatctg gcatggaaac tgactaaccg cttgatccat gcgccaacga aatcacttca
1191 acaggccgcc cgtgacgggg ataacgaacg cctgaatatt ctgcgcgaca gcctcgggct ggagtagCAG
1261 TACATCATTT TCTTTTTTTA CAGGGTGCAT TTACGCCTAT GAAGCCTTCT ATCGTTGCCA AACTGGAAGC
1331 CCTGCATGAA CGCCATGAAG AAGTTCA
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