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PEC Original Annotations
|
|
Essentiality
|
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Class
|
non-essential
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|
References (PMID)
|
Microbiology. 1996;142 (Pt 2):389-400.
The second aconitase (AcnB) of Escherichia coli.
Bradbury AJ, Gruer MJ, Rudd KE, Guest JR.
(
8932712
)
|
|
|
Deletion
|
OCR31
(D)
,
OCR31-3
(D)
,
OCR31-7
(D)
|
|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
acnB
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|
Related strains
|
|
Strains Search
|
Search strains by gene name:
acnB
Search strains by all related name:
acnB b0118 ECK0117 JW0114 yacI yacJ
|
General information (Go to Linear View: )
|
|
Gene Name
|
acnB
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Alternative name
|
b0118,ECK0117,JW0114,yacI,yacJ
|
|
Location, Length
|
131,615 - 134,212
(
+
)
;
2.84
min
;
2598
(bp) ,
865
(aa)
|
|
Product
|
bifunctional aconitate hydratase2/2-methylisocitrate dehydratase
|
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Operon Name
|
acnB
|
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Note
|
aconitate hydrase B; GO_process: GO:0006099 - tricarboxylic acid cycle; GO_process: GO:0009060 - aerobic respiration; GO_process: GO:0009061 - anaerobic respiration
|
|
Function
|
enzyme; Energy metabolism, carbon: TCA cycle
|
|
Gene Ontology
|
GO:0003994
;
aconitate hydratase activity ( acnB )
GO:0005506
;
iron ion binding ( acnB )
GO:0005515
;
protein binding ( acnB )
GO:0006099
;
tricarboxylic acid cycle ( acnB )
GO:0008152
;
metabolic process ( acnB )
GO:0016829
;
lyase activity ( acnB )
GO:0046872
;
metal ion binding ( acnB )
GO:0051536
;
iron-sulfur cluster binding ( acnB )
GO:0051539
;
4 iron, 4 sulfur cluster binding ( acnB )
|
|
PID
|
2367097
|
EC number
(KEGG Pathway)
|
4.2.1.3
4.2.1.99
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
acnB
(
EG12316
)
|
|
Number of removed
|
0 aa cleaved
|
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
ACO2_ECOLI
(
P36683
;
P36648; P75652; Q59382
)
|
|
- Protein name
|
Aconitate hydratase 2
|
|
- Synonyms
|
EC 4.2.1.3, Citrate hydro-lyase 2, Aconitase 2
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- Gene name
|
Name=acnB; OrderedLocusNames=b0118;
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Homology Analysis
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|
BLAST
|
|
Bacteria
|
|
GTOP
|
acnB
(
homologous genes of other bacterias
)
|
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PDB
(database updated :
2007.02.20
)
|
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PSI-BLAST
|
Chain A, Crystal Structure Of E. Coli Aconitase B.
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
|
Aconitate hydratase 2
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PSI-BLAST
|
Aconitate hydratase 2
|
|
nr
(database updated :
2007.02.20
)
|
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BLAST
|
COG1049: Aconitase B [Escherichia coli E22]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
|
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PROSITE
|
|
PROSITE
|
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
AMIDATION
ACONITASE_1
ACONITASE_2
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MMBR References
|
Microbiology 1994;140:2531-2541
Two genetically-distinct and differentially-regulated aconitases (AcnA and AcnB) in Escherichia coli.
Gruer, M. J., and J. R. Guest.
|
Res Microbiol. 1991;142(2-3):289-94.
Complex phenotypes of null mutations in the htr genes, whose products are essential for Escherichia coli growth at elevated temperatures.
Karow M, Raina S, Georgopoulos C, Fayet O.
(
1656498
)
|
Microbiology 1996;142:389-400
The second aconitase (AcnB) of Escherichia coli.
Bradbury, A. J., M. J. Gruer, K. E. Rudd, and J. R. Guest.
|
| Sequences |
Amino acid
FASTA format
|
0001 MLEEYRKHVA ERAAEGIAPK PLDANQMAAL VELLKNPPAG EEEFLLDLLT NRVPPGVDEA AYVKAGFLAA
0071 IAKGEAKSPL LTPEKAIELL GTMQGGYNIH PLIDALDDAK LAPIAAKALS HTLLMFDNFY DVEEKAKAGN
0141 EYAKQVMQSW ADAEWFLNRP ALAEKLTVTV FKVTGETNTD DLSPAPDAWS RPDIPLHALA MLKNAREGIE
0211 PDQPGVVGPI KQIEALQQKG FPLAYVGDVV GTGSSRKSAT NSVLWFMGDD IPHVPNKRGG GLCLGGKIAP
0281 IFFNTMEDAG ALPIEVDVSN LNMGDVIDVY PYKGEVRNHE TGELLATFEL KTDVLIDEVR AGGRIPLIIG
0351 RGLTTKAREA LGLPHSDVFR QAKDVAESDR GFSLAQKMVG RACGVKGIRP GAYCEPKMTS VGSQDTTGPM
0421 TRDELKDLAC LGFSADLVMQ SFCHTAAYPK PVDVNTHHTL PDFIMNRGGV SLRPGDGVIH SWLNRMLLPD
0491 TVGTGGDSHT RFPIGISFPA GSGLVAFAAA TGVMPLDMPE SVLVRFKGKM QPGITLRDLV HAIPLYAIKQ
0561 GLLTVEKKGK KNIFSGRILE IEGLPDLKVE QAFELTDASA ERSAAGCTIK LNKEPIIEYL NSNIVLLKWM
0631 IAEGYGDRRT LERRIQGMEK WLANPELLEA DADAEYAAVI DIDLADIKEP ILCAPNDPDD ARPLSAVQGE
0701 KIDEVFIGSC MTNIGHFRAA GKLLDAHKGQ LPTRLWVAPP TRMDAAQLTE EGYYSVFGKS GARIEIPGCS
0771 LCMGNQARVA DGATVVSTST RNFPNRLGTG ANVFLASAEL AAVAALIGKL PTPEEYQTYV AQVDKTAVDT
0841 YRYLNFNQLS QYTEKADGVI FQTAV
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CCGTTGCGGT ACTGGGCATT TACCCTACAA
-070 ACTGCTGTCT CACAGGAGCG TGAAGAGAAT CGCCTGCCGC ACTATGACAA TGAGAGCGAG GAGAACCGTC
0001 gtgctagaag aataccgtaa gcacgtagct gagcgtgccg ctgaggggat tgcgcccaaa cccctggatg
0071 caaaccaaat ggccgcactt gtagagctgc tgaaaaaccc gcccgcgggc gaagaagaat tcctgttaga
0141 tctgttaacc aaccgtgttc ccccaggcgt cgatgaagcc gcctatgtca aagcaggctt cctggctgct
0211 atcgcgaaag gcgaagccaa atcccctctg ctgactccgg aaaaagccat cgaactgctg ggcaccatgc
0281 agggtggtta caacattcat ccgctgatcg acgcgctgga tgatgccaaa ctggcaccta ttgctgccaa
0351 agcactttct cacacgctgc tgatgttcga taacttctat gacgtagaag agaaagcgaa agcaggcaac
0421 gaatatgcga agcaggttat gcagtcctgg gcggatgccg aatggttcct gaatcgcccg gcgctggctg
0491 aaaaactgac cgttactgtc ttcaaagtca ctggcgaaac taacaccgat gacctttctc cggcaccgga
0561 tgcgtggtca cgcccggata tcccactgca cgcgctggcg atgctgaaaa acgcccgtga aggtattgag
0631 ccagaccagc ctggtgttgt tggtccgatc aagcaaatcg aagctctgca acagaaaggt ttcccgctgg
0701 cgtacgtcgg tgacgttgtg ggtacgggtt cttcgcgtaa atccgccact aactccgttc tgtggtttat
0771 gggcgatgat attccacatg tgccgaacaa acgcggcggt ggtttgtgcc tcggcggtaa aattgcaccc
0841 atcttcttta acacgatgga agacgcgggt gcactgccaa tcgaagtcga cgtctctaac ctgaacatgg
0911 gcgacgtgat tgacgtttac ccgtacaaag gtgaagtgcg taaccacgaa accggcgaac tgctggcgac
0981 cttcgaactg aaaaccgacg tgctgattga tgaagtgcgt gctggtggcc gtattccgct gattatcggg
1051 cgtggcctga ccaccaaagc gcgtgaagca cttggtctgc cgcacagtga tgtgttccgt caggcgaaag
1121 atgtcgctga gagcgatcgc ggcttctcgc tggcgcaaaa aatggtaggc cgtgcctgtg gcgtgaaagg
1191 cattcgtccg ggcgcgtact gtgaaccgaa aatgacttct gtaggttccc aggacaccac cggcccgatg
1261 acccgtgatg aactgaaaga cctggcgtgc ctgggcttct cggctgacct ggtgatgcag tctttctgcc
1331 acaccgcggc gtatccgaag ccagttgacg tgaacacgca ccacacgctg ccggacttca ttatgaaccg
1401 tggcggtgtg tcgctgcgtc cgggtgacgg cgtcattcac tcctggctga accgtatgct gctgccggat
1471 accgtcggta ccggtggtga ctcccatacc cgtttcccga tcggtatctc tttcccggcg ggttctggtc
1541 tggtggcgtt tgctgccgca actggcgtaa tgccgcttga tatgccggaa tccgttctgg tgcgcttcaa
1611 aggcaaaatg cagccgggca tcaccctgcg cgatctggta cacgctattc cgctgtatgc gatcaaacaa
1681 ggtctgctga ccgttgagaa gaaaggcaag aaaaacatct tctctggccg catcctggaa attgaaggtc
1751 tgccggatct gaaagttgag caggcctttg agctaaccga tgcgtccgcc gagcgttctg ccgctggttg
1821 taccatcaag ctgaacaaag aaccgatcat cgaatacctg aactctaaca tcgtcctgct gaagtggatg
1891 atcgcggaag gttacggcga tcgtcgtacc ctggaacgtc gtattcaggg catggaaaaa tggctggcga
1961 atcctgagct gctggaagcc gatgcagatg cggaatacgc ggcagtgatc gacatcgatc tggcggatat
2031 taaagagcca atcctgtgtg ctccgaacga cccggatgac gcgcgtccgc tgtctgcggt acagggtgag
2101 aagatcgacg aagtgtttat cggttcctgc atgaccaaca tcggtcactt ccgtgctgcg ggtaaactgc
2171 tggatgcgca taaaggtcag ttgccgaccc gcctgtgggt ggcaccgcca acccgtatgg acgccgcaca
2241 gttgaccgaa gaaggctact acagcgtctt cggtaagagt ggtgcgcgta tcgagatccc tggctgttcc
2311 ctgtgtatgg gtaaccaggc gcgtgtggcg gacggtgcaa cggtggtttc cacctctacc cgtaacttcc
2381 cgaaccgtct gggtactggc gcgaatgtct tcctggcttc tgcggaactg gcggctgttg cggcgctgat
2451 tggcaaactg ccgacgccgg aagagtacca gacctacgtg gcgcaggtag ataaaacagc cgttgatact
2521 taccgttatc tgaacttcaa ccagctttct cagtacaccg agaaagccga tggggtgatt ttccagactg
2591 cggtttaaAA AGTCAGCGCA CGCGCTGCGC ATAAACGACA CAATGCCCGG TGAATGAGAT TCCCGGGCAT
2661 TTTTTTATTT CTAAACCATC GCCGTTCCGC TGTTTTTC
|
