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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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DE(nadC-aceF)72
,
DE(aroP-lpd)74
,
OCR31
(D)
,
OCR31-4
(D)
,
OCR31-7
(D)
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|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
aceE
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Related strains
|
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Strains Search
|
Search strains by gene name:
aceE
Search strains by all related name:
aceE aceE1 b0114 ECK0113 JW0110
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General information (Go to Linear View: )
|
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Gene Name
|
aceE
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|
Alternative name
|
aceE1,b0114,ECK0113,JW0110
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Location, Length
|
123,017 - 125,680
(
+
)
;
2.65
min
;
2664
(bp) ,
887
(aa)
|
|
Product
|
pyruvate dehydrogenase, decarboxylase component E1, thiamin-binding
|
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Operon Name
|
pdhR-aceEF-lpd
|
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Note
|
pyruvate dehydrogenase (decarboxylase component); GO_process: GO:0016052 - carbohydrate catabolic process; GO_process: GO:0006086 - acetyl-CoA biosynthetic process from pyruvate; GO_process: GO:0006096 - glycolysis; GO_process: GO:0009436 - glyoxylate catabolic process; GO_process: GO:0009061 - anaerobic respiration
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Function
|
enzyme; Energy metabolism, carbon: Pyruvate dehydrogenase
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Gene Ontology
|
GO:0000287
;
magnesium ion binding ( aceE )
GO:0003824
;
catalytic activity ( aceE )
GO:0004739
;
pyruvate dehydrogenase (acetyl-transferring) activity ( aceE )
GO:0005515
;
protein binding ( aceE )
GO:0005624
;
membrane fraction ( aceE )
GO:0006096
;
glycolysis ( aceE )
GO:0008152
;
metabolic process ( aceE )
GO:0016491
;
oxidoreductase activity ( aceE )
GO:0046872
;
metal ion binding ( aceE )
|
|
PID
|
1786304
|
EC number
(KEGG Pathway)
|
1.2.4.1
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
aceE
(
EG10024
)
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Number of removed
|
1 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
|
Entry name(Acc.no)
|
ODP1_ECOLI
(
P06958
;
P78049; Q53382
)
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- Protein name
|
Pyruvate dehydrogenase E1 component
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- Synonyms
|
EC 1.2.4.1
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- Gene name
|
Name=aceE; OrderedLocusNames=b0114, z0124, ECs0118;
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Homology Analysis
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BLAST
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Bacteria
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|
GTOP
|
aceE
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, E. Coli Pyruvate Dehydrogenase
|
|
SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Pyruvate dehydrogenase E1 component
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|
PSI-BLAST
|
Pyruvate dehydrogenase E1 component
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|
nr
(database updated :
2007.02.20
)
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BLAST
|
pyruvate dehydrogenase (decarboxylase component) [Escherichia coli O157:H7 EDL933]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
|
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PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
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MMBR References
|
J Gen Microbiol. 1980;121(2):277-92.
Molecular cloning of the pyruvate dehydrogenase complex genes of Escherichia coli.
Guest JR, Stephens PE.
(
6455499
)
|
J Bacteriol. 1983;153(2):588-96.
Cloning, mapping, and expression of the fumarase gene of Escherichia coli K-12.
Guest JR, Roberts RE.
(
6296045
)
|
J Gen Microbiol. 1983;129(3):671-80.
Hybrid plasmids containing the pyruvate dehydrogenase complex genes and gene-DNA relationships in the 2 to 3 minute region of the Escherichia coli chromosome.
Guest JR, Roberts RE, Stephens PE.
(
6308128
)
|
J Gen Microbiol. 1981;127:65-79.
Organization and expression of the pyruvate dehydrogenase complex genes of Escherichia coli K12.
Guest, J. R., S. T. Cole, and K. Jeyaseelan.
|
Biochem J. 1987;245(3):869-74.
Subgenes expressing single lipoyl domains of the pyruvate dehydrogenase complex of Escherichia coli.
Miles JS, Guest JR.
(
3117051
)
|
Mol Gen Genet. 1985;200(1):145-54.
Transcription analysis of the sucAB, aceEF and lpd genes of Escherichia coli.
Spencer ME, Guest JR.
(
3897791
)
|
Eur J Biochem. 1983;133:155-162
The pyruvate dehydrogenase complex of Escherichia coli K-12. Nucleotide sequence encoding the pyruvate dehydrogenase component.
Stephens, P. E., M. G. Darlison, H. M. Lewis, and J. R. Guest.
|
FEMS Microbiol Lett. 1998;159(2):325-9.
Environmental control of pyruvate dehydrogenase complex expression in Escherichia coli.
Cassey B, Guest JR, Attwood MM.
(
9503628
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSERFPNDVD PIETRDWLQA IESVIREEGV ERAQYLIDQL LAEARKGGVN VAAGTGISNY INTIPVEEQP
0071 EYPGNLELER RIRSAIRWNA IMTVLRASKK DLELGGHMAS FQSSATIYDV CFNHFFRARN EQDGGDLVYF
0141 QGHISPGVYA RAFLEGRLTQ EQLDNFRQEV HGNGLSSYPH PKLMPEFWQF PTVSMGLGPI GAIYQAKFLK
0211 YLEHRGLKDT SKQTVYAFLG DGEMDEPESK GAITIATREK LDNLVFVINC NLQRLDGPVT GNGKIINELE
0281 GIFEGAGWNV IKVMWGSRWD ELLRKDTSGK LIQLMNETVD GDYQTFKSKD GAYVREHFFG KYPETAALVA
0351 DWTDEQIWAL NRGGHDPKKI YAAFKKAQET KGKATVILAH TIKGYGMGDA AEGKNIAHQV KKMNMDGVRH
0421 IRDRFNVPVS DADIEKLPYI TFPEGSEEHT YLHAQRQKLH GYLPSRQPNF TEKLELPSLQ DFGALLEEQS
0491 KEISTTIAFV RALNVMLKNK SIKDRLVPII ADEARTFGME GLFRQIGIYS PNGQQYTPQD REQVAYYKED
0561 EKGQILQEGI NELGAGCSWL AAATSYSTNN LPMIPFYIYY SMFGFQRIGD LCWAAGDQQA RGFLIGGTSG
0631 RTTLNGEGLQ HEDGHSHIQS LTIPNCISYD PAYAYEVAVI MHDGLERMYG EKQENVYYYI TTLNENYHMP
0701 AMPEGAEEGI RKGIYKLETI EGSKGKVQLL GSGSILRHVR EAAEILAKDY GVGSDVYSVT SFTELARDGQ
0771 DCERWNMLHP LETPRVPYIA QVMNDAPAVA STDYMKLFAE QVRTYVPADD YRVLGTDGFG RSDSRENLRH
0841 HFEVDASYVV VAALGELAKR GEIDKKVVAD AIAKFNIDAD KVNPRLA
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GCAACTAAAC GTAGAACCTG TCTTATTGAG
-070 CTTTCCGGCG AGAGTTCAAT GGGACAGGTT CCAGAAAACT CAACGTTATT AGATAGATAA GGAATAACCC
0001 atgtcagaac gtttcccaaa tgacgtggat ccgatcgaaa ctcgcgactg gctccaggcg atcgaatcgg
0071 tcatccgtga agaaggtgtt gagcgtgctc agtatctgat cgaccaactg cttgctgaag cccgcaaagg
0141 cggtgtaaac gtagccgcag gcacaggtat cagcaactac atcaacacca tccccgttga agaacaaccg
0211 gagtatccgg gtaatctgga actggaacgc cgtattcgtt cagctatccg ctggaacgcc atcatgacgg
0281 tgctgcgtgc gtcgaaaaaa gacctcgaac tgggcggcca tatggcgtcc ttccagtctt ccgcaaccat
0351 ttatgatgtg tgctttaacc acttcttccg tgcacgcaac gagcaggatg gcggcgacct ggtttacttc
0421 cagggccaca tctccccggg cgtgtacgct cgtgctttcc tggaaggtcg tctgactcag gagcagctgg
0491 ataacttccg tcaggaagtt cacggcaatg gcctctcttc ctatccgcac ccgaaactga tgccggaatt
0561 ctggcagttc ccgaccgtat ctatgggtct gggtccgatt ggtgctattt accaggctaa attcctgaaa
0631 tatctggaac accgtggcct gaaagatacc tctaaacaaa ccgtttacgc gttcctcggt gacggtgaaa
0701 tggacgaacc ggaatccaaa ggtgcgatca ccatcgctac ccgtgaaaaa ctggataacc tggtcttcgt
0771 tatcaactgt aacctgcagc gtcttgacgg cccggtcacc ggtaacggca agatcatcaa cgaactggaa
0841 ggcatcttcg aaggtgctgg ctggaacgtg atcaaagtga tgtggggtag ccgttgggat gaactgctgc
0911 gtaaggatac cagcggtaaa ctgatccagc tgatgaacga aaccgttgac ggcgactacc agaccttcaa
0981 atcgaaagat ggtgcgtacg ttcgtgaaca cttcttcggt aaatatcctg aaaccgcagc actggttgca
1051 gactggactg acgagcagat ctgggcactg aaccgtggtg gtcacgatcc gaagaaaatc tacgctgcat
1121 tcaagaaagc gcaggaaacc aaaggcaaag cgacagtaat ccttgctcat accattaaag gttacggcat
1191 gggcgacgcg gctgaaggta aaaacatcgc gcaccaggtt aagaaaatga acatggacgg tgtgcgtcat
1261 atccgcgacc gtttcaatgt gccggtgtct gatgcagata tcgaaaaact gccgtacatc accttcccgg
1331 aaggttctga agagcatacc tatctgcacg ctcagcgtca gaaactgcac ggttatctgc caagccgtca
1401 gccgaacttc accgagaagc ttgagctgcc gagcctgcaa gacttcggcg cgctgttgga agagcagagc
1471 aaagagatct ctaccactat cgctttcgtt cgtgctctga acgtgatgct gaagaacaag tcgatcaaag
1541 atcgtctggt accgatcatc gccgacgaag cgcgtacttt cggtatggaa ggtctgttcc gtcagattgg
1611 tatttacagc ccgaacggtc agcagtacac cccgcaggac cgcgagcagg ttgcttacta taaagaagac
1681 gagaaaggtc agattctgca ggaagggatc aacgagctgg gcgcaggttg ttcctggctg gcagcggcga
1751 cctcttacag caccaacaat ctgccgatga tcccgttcta catctattac tcgatgttcg gcttccagcg
1821 tattggcgat ctgtgctggg cggctggcga ccagcaagcg cgtggcttcc tgatcggcgg tacttccggt
1891 cgtaccaccc tgaacggcga aggtctgcag cacgaagatg gtcacagcca cattcagtcg ctgactatcc
1961 cgaactgtat ctcttacgac ccggcttacg cttacgaagt tgctgtcatc atgcatgacg gtctggagcg
2031 tatgtacggt gaaaaacaag agaacgttta ctactacatc actacgctga acgaaaacta ccacatgccg
2101 gcaatgccgg aaggtgctga ggaaggtatc cgtaaaggta tctacaaact cgaaactatt gaaggtagca
2171 aaggtaaagt tcagctgctc ggctccggtt ctatcctgcg tcacgtccgt gaagcagctg agatcctggc
2241 gaaagattac ggcgtaggtt ctgacgttta tagcgtgacc tccttcaccg agctggcgcg tgatggtcag
2311 gattgtgaac gctggaacat gctgcacccg ctggaaactc cgcgcgttcc gtatatcgct caggtgatga
2381 acgacgctcc ggcagtggca tctaccgact atatgaaact gttcgctgag caggtccgta cttacgtacc
2451 ggctgacgac taccgcgtac tgggtactga tggcttcggt cgttccgaca gccgtgagaa cctgcgtcac
2521 cacttcgaag ttgatgcttc ttatgtcgtg gttgcggcgc tgggcgaact ggctaaacgt ggcgaaatcg
2591 ataagaaagt ggttgctgac gcaatcgcca aattcaacat cgatgcagat aaagttaacc cgcgtctggc
2661 gtaaGAGGTA AAAGAATAAT GGCTATCGAA ATCAAAGTAC CGGACATCGG GGCTGATGAA GTTGAAATCA
2731 CCGAGATCCT GGTCAAAGTG GGCGACAAAG TTGA
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