|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
|
Deletion
|
OCR63
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
purB
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
purB
Search strains by all related name:
purB ade b1131 ECK1117 JW1117
|
General information (Go to Linear View: )
|
|
Gene Name
|
purB
|
|
Alternative name
|
ade,b1131,ECK1117,JW1117
|
|
Location, Length
|
1,189,839 - 1,191,209
(
-
)
;
25.64
min
;
1371
(bp) ,
456
(aa)
|
|
Product
|
adenylosuccinate lyase
|
|
Operon Name
|
hflD-purB
|
|
Note
|
GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006164 - purine nucleotide biosynthetic process
|
|
Function
|
enzyme; Purine ribonucleotide biosynthesis
|
|
Gene Ontology
|
GO:0000034
;
adenine deaminase activity ( ade )
GO:0003824
;
catalytic activity ( purB )
GO:0004018
;
adenylosuccinate lyase activity ( purB )
GO:0005515
;
protein binding ( purB )
GO:0005737
;
cytoplasm ( purB )
GO:0006146
;
adenine catabolic process ( ade )
GO:0006164
;
purine nucleotide biosynthetic process ( purB )
GO:0009152
;
purine ribonucleotide biosynthetic process ( purB )
GO:0016787
;
hydrolase activity ( ade )
GO:0016810
;
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds ( ade )
GO:0016829
;
lyase activity ( purB )
GO:0030145
;
manganese ion binding ( ade )
|
|
PID
|
1787376
|
EC number
(KEGG Pathway)
|
4.3.2.2
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
purB
(
EG11314
)
|
|
Number of removed
|
0 aa cleaved
|
|
SWISS-PROT
(
Show details
[
1
more]
)
|
Entry name(Acc.no)
|
PUR8_ECOLI
(
P25739
)
|
|
- Protein name
|
Adenylosuccinate lyase
|
|
- Synonyms
|
EC 4.3.2.2, Adenylosuccinase, ASL
|
|
- Gene name
|
Name=purB; OrderedLocusNames=b1131, c1510;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
purB
(
homologous genes of other bacterias
)
ade
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of Adenylosuccinate Lyase From Plasmodium Vivax
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Adenylosuccinate lyase (Adenylosuccinase) (ASL)
|
|
PSI-BLAST
|
Argininosuccinate lyase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
adenylosuccinate lyase [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
AMIDATION
FUMARATE_LYASES
|
|
MMBR References
|
J Bacteriol. 1970;104(2):1027-9.
Nalidixic acid-resistant auxotrophs of Escherichia coli.
Adams BS, Helling RB.
(
4923064
)
|
Microbiology 1996;142:3219-3230
The purB gene of Escherichia coli K-12 is located in an operon.
Green, S. M., T. Malik, I. G. Giles, and W. T. Drabble.
|
J Bacteriol. 1997;179:949-951
Dihydroneopterin triphosphate epimerase of Escherichia coli: purification, genetic cloning, and expression.
Haussmann, C., F. Rohdich, F. Lottspeich, S. Eberhardt, J. Scheuring, S. Mackamul, and A. Bacher.
|
J Bacteriol. 1992;174:130-136
Escherichia coli purB gene: cloning, nucleotide sequence, and regulation by purR.
He, B., J. M. Smith, and H. Zalkin.
|
J Bacteriol. 1990;172(3):1173
Location of purine genes on the physical map of Escherichia coli.
Masters M.
(
2407714
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MELSSLTAVS PVDGRYGDKV SALRGIFSEY GLLKFRVQVE VRWLQKLAAH AAIKEVPAFA ADAIGYLDAI
0071 VASFSEEDAA RIKTIERTTN HDVKAVEYFL KEKVAEIPEL HAVSEFIHFA CTSEDINNLS HALMLKTARD
0141 EVILPYWRQL IDGIKDLAVQ YRDIPLLSRT HGQPATPSTI GKEMANVAYR MERQYRQLNQ VEILGKINGA
0211 VGNYNAHIAA YPEVDWHQFS EEFVTSLGIQ WNPYTTQIEP HDYIAELFDC VARFNTILID FDRDVWGYIA
0281 LNHFKQKTIA GEIGSSTMPH KVNPIDFENS EGNLGLSNAV LQHLASKLPV SRWQRDLTDS TVLRNLGVGI
0351 GYALIAYQST LKGVSKLEVN RDHLLDELDH NWEVLAEPIQ TVMRRYGIEK PYEKLKELTR GKRVDAEGMK
0421 QFIDGLALPE EEKARLKAMT PANYIGRAIT MVDELK
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ccaggtcggc ggcggacgtc tgcaactgat
-070 gttttctcgt aatcgcctga ccactcaggc aaaacaaatt cttgctcatt taaccccgga gttgtgatct
0001 atggaattat cctcactgac cgccgtttcc cctgtcgatg gacgctacgg cgataaagtc agcgcgctgc
0071 gcgggatttt cagcgaatat ggtttgctga aattccgtgt acaagttgaa gtacgttggc tgcaaaaact
0141 ggccgcgcac gcagcgatca aggaagttcc tgcttttgct gccgacgcaa tcggttacct tgatgcaatc
0211 gtcgccagtt tcagcgaaga agatgcggcg cgcatcaaaa ctatcgagcg taccactaac cacgacgtta
0281 aagcggttga gtatttcctg aaagaaaaag tggcggagat cccggaactg cacgcggttt ctgaattcat
0351 ccactttgcc tgtacttcgg aagatatcaa taacctctcc cacgcattaa tgctgaaaac cgcgcgtgat
0421 gaagtgatcc tgccatactg gcgtcaactg attgatggca ttaaagatct cgccgttcag tatcgcgata
0491 tcccgctgct gtctcgtacc cacggtcagc cagccacgcc gtcaaccatc ggtaaagaga tggcaaacgt
0561 cgcctaccgt atggagcgcc agtaccgcca gcttaaccag gtggagatcc tcggcaaaat caacggcgcg
0631 gtcggtaact ataacgccca catcgccgct tacccggaag ttgactggca tcagttcagc gaagagttcg
0701 tcacctcgct gggtattcag tggaacccgt acaccaccca gatcgaaccg cacgactaca ttgccgaact
0771 gtttgattgc gttgcgcgct tcaacactat tctgatcgac tttgaccgtg acgtctgggg ttatatcgcc
0841 cttaaccact tcaaacagaa aaccattgct ggtgagattg gttcttccac catgccgcat aaagttaacc
0911 cgatcgactt cgaaaactcc gaagggaatc tgggcctttc caacgcggta ttgcagcatc tggcaagcaa
0981 actgccggtt tcccgctggc agcgtgacct gaccgactct accgtgctgc gtaacctcgg cgtgggtatc
1051 ggttatgcct tgattgcata tcaatccacc ctgaaaggcg tgagcaaact ggaagtgaac cgtgaccatc
1121 tgctggatga actggatcac aactgggaag tgctggctga accaatccag acagttatgc gtcgctatgg
1191 catcgaaaaa ccgtacgaga agctgaaaga gctgactcgc ggtaagcgcg ttgacgccga aggcatgaag
1261 cagtttatcg atggtctggc gttgccagaa gaagagaaag cccgcctgaa agcgatgacg ccggctaact
1331 atattggtcg agctatcacg atggttgatg agctgaaata aacctcgtat cagtgccgga tggcgatgct
1401 gtccggcctg cttattaaga ttatccgctt tttatttttt cactttacct cccctccccg ctggtttatt
1471 t
|
