MG1655
W3110
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Gene Report : thiK
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion OCR62-1 (D)  ,  OCR62-6 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  thiK
Related strains
     Strains Search Search strains by gene name:  thiK   Search strains by all related name:  thiK b1106 ECK1092 JW1092 o274 o274a ycfN

General information  (Go to Linear View:)
 Gene Name thiK  
 Alternative name b1106,ECK1092,JW1092,o274,o274a,ycfN  
 Location, Length 1,162,483 - 1,163,307 (  +  ) ;   25.06 min ; 825 (bp) ,   274 (aa) Go to Linear View
 Product thiamin kinase  
 Operon Name hinT-ycfL-lpoB-thiK-nagZ-ycfP  
 Note putative beta-glucosidase  
 Function enzyme; Biosynthesis of cofactors, carriers: Thiamin  
 Gene Ontology GO:0016301 ; kinase activity ( thiK )
GO:0016310 ; phosphorylation ( thiK )
GO:0016740 ; transferase activity ( thiK )
GO:0019165 ; thiamin kinase activity ( thiK )
 PID 1787349  
 EC number
  (KEGG Pathway)
 
2.7.1.89  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) YCFN_ECOLI ( P75948 )
    -  Protein name Hypothetical protein ycfN.  
    -  Synonyms  
    -  Gene name Name=ycfN; OrderedLocusNames=b1106;  

 Linear View (Whole Mode)
View Location
   1150.0  –  1175.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP ycfN (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
        PSI-BLAST  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Thiamine kinase  
         PSI-BLAST Thiamine kinase
    nr     (database updated : 2007.02.20 )
         BLAST thiamin kinase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG0510M 
    EcoCyc thiK 

MMBR References
J Bacteriol. 1982;151(2):708-17.
thiK and thiL loci of Escherichia coli.
Imamura N, Nakayama H. ( 6284709 )
J Bacteriol. 1984;160(2):711-7.
sn-Glycerol-3-phosphate auxotrophy of plsB strains of Escherichia coli: evidence that a second mutation, plsX, is required.
Larson TJ, Ludtke DN, Bell RM. ( 6094487 )

Sequences
Amino acid
FASTA format
0001 MPFRSNNPIT RDELLSRFFP QYHPVTTFNS GLSGGSFLIE HQGQRFVVRQ PHDPDAPQSA FLRQYRALSQ 
0071 LPACIAPKPH LYLRDWMVVD YLPGAVKTYL PDTNELAGLL YYLHQQPRFG WRITLLPLLE LYWQQSDPAR 
0141 RTVGWLRMLK RLRKAREPRP LRLSPLHMDV HAGNLVHSAS GLKLIDWEYA GDGDIALELA AVWVENTEQH 
0211 RQLVNDYATR AKIYPAQLWR QVRRWFPWLL MLKAGWFEYR WRQTGDQQFI RLADDTWRQL LIKQ

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             TACGTGCTGT ACTCCAGCGC CTCTGGCAAC 
-070 GTTAACGCTC CGACCCTACA AATGCAGCTG ATGCTGGTGC AGACGGGCGA AATTATCTGG TCAGGTAAAG 
0001 gtgccgtttc gcagcaataa tcccatcacg cgcgacgaat tgctgtcgcg ctttttcccg cagtatcatc 
0071 ccgtcacgac gtttaatagt gggcttagtg gcgggagttt tctcattgaa catcagggcc agcgttttgt 
0141 tgtgcgtcag ccgcacgatc ctgatgcgcc gcagtccgcg ttcttgcgcc agtatcgggc tttatcacaa 
0211 ctacccgcat gcattgcacc gaagccgcat ttatatctcc gtgactggat ggtagtcgac tatctgcccg 
0281 gcgcggtaaa aacgtatttg ccggatacca acgaactggc aggcttgctg tattatctac atcaacaacc 
0351 acgttttggc tggcgaataa cgctgttgcc gttactggaa ctgtactggc agcaaagcga tccggcgcgg 
0421 cggacagtgg gttggctgcg aatgttaaaa cgtctgcgca aagcgcggga accacggcct ttacgcttaa 
0491 gtccattgca tatggatgtc cacgccggaa atttagtgca tagcgcgtca gggttaaaac tcatcgactg 
0561 ggagtatgcc ggagatggtg atatcgcgct ggaactggcg gcggtgtggg tggaaaatac tgaacagcac 
0631 cggcaattgg tcaatgacta tgccactcgc gcgaagattt atccggcgca attatggcgt caggtcaggc 
0701 gatggtttcc ctggctgctg atgctcaaag cagggtggtt tgagtaccgc tggcgacaaa ccggcgatca 
0771 acaatttatc aggctggccg atgacacctg gcggcagcta ttaataaaac aataaGGAGA GCAGTGTGGG 
0841 TCCAGTAATG TTGGATGTCG AAGGTTACGA ACTGGACGCG GAAGAGCGTG AAATACTGGC GCATCCGCTG 
0911 GTGGGAGGGC TGATT