MG1655
W3110
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Gene Report : ptsG
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR62-1 (D)  ,  OCR62-6 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  ptsG
Related strains
     Strains Search Search strains by gene name:  ptsG   Search strains by all related name:  ptsG b1101 car cat CR ECK1087 glcA gpt JW1087 tgl umg umgC

General information  (Go to Linear View:)
 Gene Name ptsG  
 Alternative name b1101,car,cat,CR,ECK1087,glcA,gpt,JW1087,tgl,umg,umgC  
 Location, Length 1,157,092 - 1,158,525 (  +  ) ;   24.94 min ; 1434 (bp) ,   477 (aa) Go to Linear View
 Product fused glucose-specific PTS enzymes: IIBcomponent/IIC component  
 Operon Name ptsG  
 Note PTS system, glucose-specific IIBC component; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0016052 - carbohydrate catabolic process; GO_process: GO:0006464 - protein modification process  
 Function transport; Transport of small molecules: Carbohydrates, organic acids, alcohols  
 Gene Ontology GO:0000287 ; magnesium ion binding ( gpt )
GO:0000310 ; xanthine phosphoribosyltransferase activity ( gpt )
GO:0005351 ; sugar:hydrogen ion symporter activity ( ptsG )
GO:0005355 ; glucose transmembrane transporter activity ( ptsG )
GO:0005515 ; protein binding ( gpt )
GO:0005515 ; protein binding ( ptsG )
GO:0005737 ; cytoplasm ( gpt )
GO:0005886 ; plasma membrane ( glcA )
GO:0005886 ; plasma membrane ( ptsG )
GO:0006166 ; purine ribonucleoside salvage ( gpt )
GO:0006810 ; transport ( glcA )
GO:0006810 ; transport ( ptsG )
GO:0008643 ; carbohydrate transport ( ptsG )
GO:0008982 ; protein-N(PI)-phosphohistidine-sugar phosphotransferase activity ( ptsG )
GO:0009116 ; nucleoside metabolic process ( gpt )
GO:0009401 ; phosphoenolpyruvate-dependent sugar phosphotransferase system ( ptsG )
GO:0015129 ; lactate transmembrane transporter activity ( glcA )
GO:0015727 ; lactate transport ( glcA )
GO:0015758 ; glucose transport ( ptsG )
GO:0016020 ; membrane ( glcA )
GO:0016020 ; membrane ( ptsG )
GO:0016021 ; integral to membrane ( glcA )
GO:0016021 ; integral to membrane ( ptsG )
GO:0016301 ; kinase activity ( ptsG )
GO:0016740 ; transferase activity ( gpt )
GO:0016740 ; transferase activity ( ptsG )
GO:0016757 ; transferase activity, transferring glycosyl groups ( gpt )
GO:0046872 ; metal ion binding ( gpt )
 PID 1787343  
 EC number
  (KEGG Pathway)   		2.7.1.69  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene ptsG ( EG10787 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) PTGB_ECOLI ( P05053 )
    -  Protein name PTS system, glucose-specific IIBC component  
    -  Synonyms EIIBC-Glc, Glucose- permease IIBC component, Phosphotransferase enzyme II, BC component, EC 2.7.1.69, EII-Glc  
    -  Gene name OrderedLocusNames=b1101, c1373, z1740, ECs1479;  

 Linear View (Whole Mode)
View Location
   1145.0  –  1170.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP gpt (  homologous genes of other bacterias  )   ptsG (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain B, Complex Of Enzyme Iiaglc And Iibglc Phosphocarrier Protein Hpr From Escherichia Coli Nmr, Restrained Regularized Mean Structure  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST PTS system glucose-specific EIICB component  
         PSI-BLAST PTS system glucose-specific EIICB component
    nr     (database updated : 2007.02.20 )
         BLAST COG1263: Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific [Escherichia coli E22]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AMIDATION    LEUCINE_ZIPPER    PTS_EIIA_1    PTS_EIIB_CYS     

Other Cross-Reference
    COG COG1263G 
    EcoCyc ptsG 

MMBR References
J Biol Chem. 1986;261:16398-16403
Glucose-permease of the bacterial phosphotransferase system. Gene cloning, overproduction and amino acid sequence of enzyme IIGlc.
Erni, B., and B. Zanolari.
Proc Natl Acad Sci U S A. 1997;94:12914-12919
cAMP receptor protein-cAMP plays a crucial role in glucose-lactose diauxie by activating the major glucose transporter gene in Escherichia coli.
Kimata, K., H. Takahashi, T. Inada, P. Postma, and H. Aiba.
Mol Gen Genet. 1984;197(1):175-81.
Mapping of markers in the gyrA-his region of Escherichia coli.
Middendorf A, Schweizer H, Vreemann J, Boos W. ( 6392823 )
J Bacteriol. 1992;174:2843-2850
Analysis of mutations that uncouple transport from phosphorylation in enzyme IIGlc of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system.
Ruijter, G. J., G. van Meurs, M. A. Verwey, P. W. Postma, and K. van Dam.
Biochim Biophys Acta. 1981;646(2):365-7.
L-Sorbose phosphorylation in Escherichia coli K-12.
Slater AC, Jones-Mortimer MC, Kornberg HL. ( 7028117 )
Proc Natl Acad Sci U S A. 1987;84:930-934
II-BGlc, a glucose receptor of the bacterial phosphotransferase system: molecular cloning of ptsG and purification of the receptor from an overproducing strain of Escherichia coli.
Bouma, C. L., N. D. Meadow, E. W. Stover, and S. Roseman.
J. Biochem. (Tokyo) 1975;53:419-427.
Glucose catabolite repression in Escherichia coli K12 mutants defective in methyl-a-D-glucose transport.
Bourd, G., R. S. Erlagaeva, T. N. Bolshakova, and V. N. Gershanovich.
J Biol Chem. 1992;267:3847-3851
The glucose transporter of Escherichia coli. Mutants with impaired translocation activity that retain phosphorylation activity.
Buhr, A., G. A. Daniels, and B. Erni.
J Bacteriol. 1975;122(3):1189-99.
Phosphorylation of D-glucose in Escherichia coli mutants defective in glucosephosphotransferase, mannosephosphotransferase, and glucokinase.
Curtis SJ, Epstein W. ( 1097393 )

Sequences
Amino acid
FASTA format 
0001 MFKNAFANLQ KVGKSLMLPV SVLPIAGILL GVGSANFSWL PAVVSHVMAE AGGSVFANMP LIFAIGVALG 
0071 FTNNDGVSAL AAVVAYGIMV KTMAVVAPLV LHLPAEEIAS KHLADTGVLG GIISGAIAAY MFNRFYRIKL 
0141 PEYLGFFAGK RFVPIISGLA AIFTGVVLSF IWPPIGSAIQ TFSQWAAYQN PVVAFGIYGF IERCLVPFGL 
0211 HHIWNVPFQM QIGEYTNAAG QVFHGDIPRY MAGDPTAGKL SGGFLFKMYG LPAAAIAIWH SAKPENRAKV 
0281 GGIMISAALT SFLTGITEPI EFSFMFVAPI LYIIHAILAG LAFPICILLG MRDGTSFSHG LIDFIVLSGN 
0351 SSKLWLFPIV GIGYAIVYYT IFRVLIKALD LKTPGREDAT EDAKATGTSE MAPALVAAFG GKENITNLDA 
0421 CITRLRVSVA DVSKVDQAGL KKLGAAGVVV AGSGVQAIFG TKSDNLKTEM DEYIRNH
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             AATAAAGGGC GCTTAGATGC CCTGTACACG 
-070 GCGAGGCTCT CCCCCCTTGC CACGCGTGAG AACGTAAAAA AAGCACCCAT ACTCAGGAGC ACTCTCAATT 
0001 atgtttaaga atgcatttgc taacctgcaa aaggtcggta aatcgctgat gctgccggta tccgtactgc 
0071 ctatcgcagg tattctgctg ggcgtcggtt ccgcgaattt cagctggctg cccgccgttg tatcgcatgt 
0141 tatggcagaa gcaggcggtt ccgtctttgc aaacatgcca ctgatttttg cgatcggtgt cgccctcggc 
0211 tttaccaata acgatggcgt atccgcgctg gccgcagttg ttgcctatgg catcatggtt aaaaccatgg 
0281 ccgtggttgc gccactggta ctgcatttac ctgctgaaga aatcgcctct aaacacctgg cggatactgg 
0351 cgtactcgga gggattatct ccggtgcgat cgcagcgtac atgtttaacc gtttctaccg tattaagctg 
0421 cctgagtatc ttggcttctt tgccggtaaa cgctttgtgc cgatcatttc tggcctggct gccatcttta 
0491 ctggcgttgt gctgtccttc atttggccgc cgattggttc tgcaatccag accttctctc agtgggctgc 
0561 ttaccagaac ccggtagttg cgtttggcat ttacggtttc atcgaacgtt gcctggtacc gtttggtctg 
0631 caccacatct ggaacgtacc tttccagatg cagattggtg aatacaccaa cgcagcaggt caggttttcc 
0701 acggcgacat tccgcgttat atggcgggtg acccgactgc gggtaaactg tctggtggct tcctgttcaa 
0771 aatgtacggt ctgccagctg ccgcaattgc tatctggcac tctgctaaac cagaaaaccg cgcgaaagtg 
0841 ggcggtatta tgatctccgc ggcgctgacc tcgttcctga ccggtatcac cgagccgatc gagttctcct 
0911 tcatgttcgt tgcgccgatc ctgtacatca tccacgcgat tctggcaggc ctggcattcc caatctgtat 
0981 tcttctgggg atgcgtgacg gtacgtcgtt ctcgcacggt ctgatcgact tcatcgttct gtctggtaac 
1051 agcagcaaac tgtggctgtt cccgatcgtc ggtatcggtt atgcgattgt ttactacacc atcttccgcg 
1121 tgctgattaa agcactggat ctgaaaacgc cgggtcgtga agacgcgact gaagatgcaa aagcgacagg 
1191 taccagcgaa atggcaccgg ctctggttgc tgcatttggt ggtaaagaaa acattactaa cctcgacgca 
1261 tgtattaccc gtctgcgcgt cagcgttgct gatgtgtcta aagtggatca ggccggcctg aagaaactgg 
1331 gcgcagcggg cgtagtggtt gctggttctg gtgttcaggc gattttcggt actaaatccg ataacctgaa 
1401 aaccgagatg gatgagtaca tccgtaacca ctaaTCCGTA AGACGTTGGG GAGACTAAGG CAGCCAGATG 
1471 GCTGCCTTTT TTACAGGTGT TATTCAGAAT TGATACGTGC CGGTAATGCT GAAATTACGC GGTG