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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Bacteriol. 1994;176(3):804-14.
Genetic and molecular characterization of the Escherichia coli secD operon and its products.
Pogliano KJ, Beckwith J.
(
7507921
)
|
|
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Deletion
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OCR39
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
secF
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Related strains
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Strains Search
|
Search strains by gene name:
secF
Search strains by all related name:
secF b0409 ECK0403 JW0399
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General information (Go to Linear View: )
|
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Gene Name
|
secF
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Alternative name
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b0409,ECK0403,JW0399
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Location, Length
|
428,729 - 429,700
(
+
)
;
9.24
min
;
972
(bp) ,
323
(aa)
|
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Product
|
SecYEG protein translocase auxillary subunit
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Operon Name
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tgt-yajC-secDF
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Note
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protein secretion, membrane protein; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane
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Function
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transport; Protein, peptide secretion
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Gene Ontology
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GO:0005886
;
plasma membrane ( secF )
GO:0006810
;
transport ( secF )
GO:0006886
;
intracellular protein transport ( secF )
GO:0008565
;
protein transporter activity ( secF )
GO:0009276
;
1-2nm peptidoglycan-based cell wall ( secF )
GO:0015031
;
protein transport ( secF )
GO:0015450
;
P-P-bond-hydrolysis-driven protein transmembrane transporter activity ( secF )
GO:0015627
;
type II protein secretion system complex ( secF )
GO:0015628
;
protein secretion by the type II secretion system ( secF )
GO:0016020
;
membrane ( secF )
GO:0016021
;
integral to membrane ( secF )
GO:0065002
;
intracellular protein transport across a membrane ( secF )
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PID
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1786610
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EC number
(KEGG Pathway)
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SWISS-PROT
(
Show details
)
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Entry name(Acc.no)
|
SECF_ECOLI
(
P19674
;
P77113
)
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- Protein name
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Protein-export membrane protein secF.
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- Synonyms
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- Gene name
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Name=secF; OrderedLocusNames=b0409, c0519, z0508, ECs0460;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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secF
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Protein-export membrane protein secF
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PSI-BLAST
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Protein-export membrane protein secF
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nr
(database updated :
2007.02.20
)
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BLAST
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protein secretion, membrane protein [Shigella dysenteriae Sd197]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
AMIDATION
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MMBR References
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J Bioenerg Biomembr. 1990;22(3):369-87.
Protein translocation in vitro: biochemical characterization of genetically defined translocation components.
Fandl J, Tai PC.
(
2167893
)
|
EMBO J. 1990;9:3209-3216
The secD locus of E. coli codes for two membrane proteins required for protein export.
Gardel, C., K. R. Johnson, A. Jacq, and J. R. Beckwith.
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J Bacteriol. 1993;175:6113-6117
Processing of lipid-modified prolipoprotein requires energy and sec gene products in vivo.
Kosic, N., M. Sugai, C. K. Fan, and H. C. Wu.
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J Bacteriol. 1992;174(5):1709-10.
Alignment of genes from the 9-minute region (araJ to tsx) of the Escherichia coli K-12 linkage map to the physical map.
Lucht JM, Boos W, Bremer E.
(
1537816
)
|
Biochim Biophys Acta. 1992;1122(1):77-84.
Overproduction, purification and characterization of SecD and SecF, integral membrane components of the protein translocation machinery of Escherichia coli.
Matsuyama S, Fujita Y, Sagara K, Mizushima S.
(
1633199
)
|
EMBO J. 1994;13:554-561
SecD and SecF facilitate protein export in Escherichia coli.
Pogliano, J. A., and J. R. Beckwith.
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J Bacteriol. 1994;176:804-814
Genetic and molecular characterization of the Escherichia coli secD operon and its products.
Pogliano, K. J., and J. R. Beckwith.
|
J Bacteriol. 1994;176:4111-4116
SecF stabilizes SecD and SecY, components of the protein translocation machinery of the Escherichia coli cytoplasmic membrane.
Sagara, K., S. Matsuyama, and S. Mizushima.
|
J Bacteriol. 1992;174:2511-2516
Export of the outer membrane lipoprotein is defective in secD, secE, and secF mutants of Escherichia coli.
Sugai, M., and H. C. Wu.
|
J Bacteriol. 1995;177:4969-4973
Multicopy suppression of cold-sensitive sec mutations in Escherichia coli.
Danese, P. N., C. K. Murphy, and T. J. Silhavy.
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| Sequences |
Amino acid
FASTA format
|
0001 MAQEYTVEQL NHGRKVYDFM RWDYWAFGIS GLLLIAAIVI MGVRGFNWGL DFTGGTVIEI TLEKPAEIDV
0071 MRDALQKAGF EEPMLQNFGS SHDIMVRMPP AEGETGGQVL GSQVLKVINE STNQNAAVKR IEFVGPSVGA
0141 DLAQTGAMAL MAALLSILVY VGFRFEWRLA AGVVIALAHD VIITLGILSL FHIEIDLTIV ASLMSVIGYS
0211 LNDSIVVSDR IRENFRKIRR GTPYEIFNVS LTQTLHRTLI TSGTTLMVIL MLYLFGGPVL EGFSLTMLIG
0281 VSIGTASSIY VASALALKLG MKREHMLQQK VEKEGADQPS ILP
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GCGACGTCGA TGTTTACCGC GATTGTCGGT
-070 ACGCGTGCCA TCGTAAACCT GCTATATGGC GGCAAGCGCG TCAAGAAGCT GTCAATCTGA GGAGTGCGAT
0001 gtggcacagg aatatactgt tgaacaacta aaccacggcc gtaaagtcta tgactttatg cgctgggact
0071 actgggcttt cggcatctct ggtctgctgt taatcgctgc tatcgttatt atgggcgtgc gcggctttaa
0141 ctgggggctg gatttcaccg gtggtacggt tattgaaatt acgctcgaaa aaccggctga aattgacgta
0211 atgcgtgatg cattgcaaaa agccggtttt gaagagccga tgctgcaaaa ctttggtagc agccatgaca
0281 tcatggtccg tatgccgcct gctgaaggcg aaaccggcgg tcaggtgttg ggcagccagg ttctgaaggt
0351 gattaacgaa tccaccaatc agaatgcagc agtgaagcgt attgagttcg tcggtccgag cgtgggggca
0421 gaccttgcgc aaaccggtgc gatggcgttg atggcagcgc tgctgtctat cctcgtgtac gtaggtttcc
0491 gctttgagtg gcgactggcg gcaggggtgg ttattgcgct ggcgcacgac gttatcatta cgctgggtat
0561 tttgtcgtta ttccatatcg agattgacct gaccattgtg gcatcgttga tgtcggttat cggttactcg
0631 cttaacgaca gtatcgtggt atcggaccgt attcgtgaaa acttccgcaa gatccgtcgc ggtacgcctt
0701 acgaaatctt taacgtgtcc ttgacccaga cgctgcaccg taccttgatc acatccggta ctaccttgat
0771 ggttatcctg atgctgtacc tcttcggtgg tccggtactg gaaggcttct cgctgaccat gcttatcggt
0841 gtttccatcg gtactgcatc ttccatctat gtggcatctg cgttggctct gaaactgggt atgaagcgcg
0911 aacacatgtt gcagcagaaa gtggaaaaag aaggggcgga tcagccgtca attctgccgt aaTCAAGTTC
0981 CCGTTGATGT TGAAAATCCC GGTCAGAAGA TCGGGATTTT TTTTGATGTA TGGAGATTGC GAAAATCCCC
1051 GCATCTTGGG AAACTGCGCG TA
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