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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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|
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Deletion
|
OCL64
(D)
,
OCL64-10
(D)
|
|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
truA
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Related strains
|
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Strains Search
|
Search strains by gene name:
truA
Search strains by all related name:
truA asuC b2318 ECK2312 hisT JW2315 leuK
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General information (Go to Linear View: )
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Gene Name
|
truA
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|
Alternative name
|
asuC,b2318,ECK2312,hisT,JW2315,leuK
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Location, Length
|
2,432,846 - 2,433,658
(
-
)
;
52.44
min
;
813
(bp) ,
270
(aa)
|
|
Product
|
tRNA pseudouridine(38-40) synthase
|
|
Operon Name
|
pdxB-usg-truA-dedA
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Note
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GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006418 - tRNA aminoacylation for protein translation
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Function
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enzyme; tRNA modification
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Gene Ontology
|
GO:0000049
;
tRNA binding ( truA )
GO:0001522
;
pseudouridine synthesis ( truA )
GO:0008033
;
tRNA processing ( truA )
GO:0016439
;
tRNA-pseudouridine synthase activity ( truA )
GO:0016853
;
isomerase activity ( truA )
GO:0031119
;
tRNA pseudouridine synthesis ( truA )
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|
PID
|
1788657
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EC number
(KEGG Pathway)
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4.2.1.70
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
|
truA
(
EG10454
)
|
|
Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
|
Entry name(Acc.no)
|
TRUA_ECOLI
(
P07649
)
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- Protein name
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tRNA pseudouridine synthase A
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- Synonyms
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EC 4.2.1.70, Pseudouridylate synthase I, Pseudouridine synthase I, Uracil hydrolyase, PSU-I
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- Gene name
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Name=truA; Synonyms=hisT, asuC, leuK; OrderedLocusNames=b2318;
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MMBR References
|
Nucleic Acids Res. 1985;13:5297-5315
Structural features of the hisT operon of Escherichia coli K-12.
Arps, P. J., C. C. Marvel, B. C. Rubin, D. A. Tolan, E. E. Penhoet, and M. E. Winkler.
|
J Bacteriol. 1987;169(3):1071-9.
An unusual genetic link between vitamin B6 biosynthesis and tRNA pseudouridine modification in Escherichia coli K-12.
Arps PJ, Winkler ME.
(
3029017
)
|
J Bacteriol. 1987;169(3):1061-70.
Structural analysis of the Escherichia coli K-12 hisT operon by using a kanamycin resistance cassette.
Arps PJ, Winkler ME.
(
3029016
)
|
J Bacteriol. 1985;161(1):60-71.
hisT is part of a multigene operon in Escherichia coli K-12.
Marvel CC, Arps PJ, Rubin BC, Kammen HO, Penhoet EE, Winkler ME.
(
2981810
)
|
J Biol Chem. 1987;262:12209-12217
The hisT-purF region of the Escherichia coli K-12 chromosome. Identification of additional genes of the hisT and purF operons.
Nonet, M. L., C. C. Marvel, and D. R. Tolan.
|
RNA 1995;1:102-112
Purification, cloning, and properties of the tRNA psi-55 synthase from Escherichia coli.
Nurse, K., J. Wrzesinski, A. Bakin, B. G. Lane, and J. Ofengand.
|
Mol Gen Genet. 1982;187(3):405-9.
Specific mistranslation in hisT mutants of Escherichia coli.
Parker J.
(
6757659
)
|
J Bacteriol. 1986;166(1):341-5.
Escherichia coli B/r leuK mutant lacking pseudouridine synthase I activity.
Searles LL, Jones JW, Fournier MJ, Grambow N, Tyler B, Calvo JM.
(
3514581
)
|
Mol Gen Genet. 1978;165(1):21-30.
Correlation between the serine sensitivity and the derepressibility of the ilv genes in Escherichia coli relA- mutants.
Uzan M, Danchin A.
(
362163
)
|
J Bacteriol. 1977;130(1):4-10.
Biochemical and regulatory properties of Escherichia coli K-12 hisT mutants.
Bruni CB, Colantuoni V, Sbordone L, Cortese R, Blasi F.
(
323237
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSDQQQPPVY KIALGIEYDG SKYYGWQRQN EVRSVQEKLE KALSQVANEP ITVFCAGRTD AGVHGTGQVV
0071 HFETTALRKD AAWTLGVNAN LPGDIAVRWV KTVPDDFHAR FSATARRYRY IIYNHRLRPA VLSKGVTHFY
0141 EPLDAERMHR AAQCLLGEND FTSFRAVQCQ SRTPWRNVMH INVTRHGPYV VVDIKANAFV HHMVRNIVGS
0211 LMEVGAHNQP ESWIAELLAA KDRTLAAATA KAEGLYLVAV DYPDRYDLPK PPMGPLFLAD
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 agcaagtcca gttctggtcg gtggccgata
-070 acgttcgctt tggcggcgcg ctgatggcag taaaaatcgc cgagaaactg gtgcaggagt atctgtacta
0001 atgtccgacc agcaacaacc gccagtttat aaaattgcgc tgggcattga gtacgacggc agtaagtatt
0071 acggctggca acggcagaac gaagtccgca gtgtgcagga gaagctggaa aaggcgctct cccaggtggc
0141 gaacgaaccc atcaccgtct tctgcgccgg gcgtactgac gcaggggtac acggtaccgg gcaggttgtg
0211 catttcgaaa caaccgcgct gcgcaaagac gcggcgtgga ccttgggcgt aaatgcgaat ttacctggtg
0281 acatcgctgt gcgttgggtt aaaactgtac ctgatgattt tcatgcccga tttagcgcca cggctcgccg
0351 ttatcgctac atcatctaca atcatcggct gcgcccggcg gtactgagta aaggggtaac ccatttttac
0421 gaaccgctgg acgctgaacg gatgcatcgg gctgcgcaat gcttgctggg cgagaatgat ttcacctcgt
0491 tccgtgcggt gcagtgccag tcccgaaccc cgtggcgcaa cgttatgcat attaatgtca cgcgtcacgg
0561 tccttatgtg gtggtagata ttaaagcgaa tgcttttgta catcatatgg tcaggaatat tgtcggcagc
0631 ctgatggaag tcggtgccca caaccagccg gagagctgga tagcagagtt gctggcggca aaggacagaa
0701 cgctggcggc agcaacggca aaagcggaag ggctgtatct ggtcgcggtg gattaccctg accggtatga
0771 tcttccaaaa ccgccaatgg gcccgctatt tctggcggac taacgagaat aatgctcgga acatttcagg
0841 ctgtagccaa cgtagttaca gcctgaaaga tgacgagtac aaggcatagg caaatatgga cctgatttat
0911 ttc
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