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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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|
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Deletion
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DE(xthA-pncA)90
,
LD3-17-1
(LD)
,
OCL84,85-1
(D)
|
|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
ansA
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Related strains
|
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Strains Search
|
Search strains by gene name:
ansA
Search strains by all related name:
ansA b1767 ECK1765 JW1756
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General information (Go to Linear View: )
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Gene Name
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ansA
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Alternative name
|
b1767,ECK1765,JW1756
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Location, Length
|
1,848,884 - 1,849,900
(
+
)
;
39.85
min
;
1017
(bp) ,
338
(aa)
|
|
Product
|
cytoplasmic L-asparaginase I
|
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Operon Name
|
ansA-pncA
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Note
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GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009063 - cellular amino acid catabolic process
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Function
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enzyme; Degradation of small molecules: Amino acids
|
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Gene Ontology
|
GO:0004067
;
asparaginase activity ( ansA )
GO:0005737
;
cytoplasm ( ansA )
GO:0006520
;
amino acid metabolic process ( ansA )
GO:0016787
;
hydrolase activity ( ansA )
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|
PID
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1788065
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EC number
(KEGG Pathway)
|
3.5.1.1
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
|
ASG1_ECOLI
(
P18840
)
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- Protein name
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L-asparaginase I
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- Synonyms
|
EC 3.5.1.1, L-asparagine amidohydrolase I, L- ASNase I
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- Gene name
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Name=ansA; OrderedLocusNames=b1767, z2801, ECs2474;
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MMBR References
|
J Bacteriol. 1983;154(1):513-5.
Cytoplasmic L-asparaginase: isolation of a defective strain and mapping of ansA.
Del Casale T, Sollitti P, Chesney RH.
(
6339481
)
|
J Bacteriol. 1986;166(1):135-42.
L-asparaginase genes in Escherichia coli: isolation of mutants and characterization of the ansA gene and its protein product.
Spring KJ, Jerlstrom PG, Burns DM, Beacham IR.
(
3514575
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MQKKSIYVAY TGGTIGMQRS EQGYIPVSGH LQRQLALMPE FHRPEMPDFT IHEYTPLMDS SDMTPEDWQH
0071 IAEDIKAHYD DYDGFVILHG TDTMAYTASA LSFMLENLGK PVIVTGSQIP LAELRSDGQI NLLNALYVAA
0141 NYPINEVTLF FNNRLYRGNR TTKAHADGFD AFASPNLPPL LEAGIHIRRL NTPPAPHGEG ELIVHPITPQ
0211 PIGVVTIYPG ISADVVRNFL RQPVKALILR SYGVGNAPQN KAFLQELQEA SDRGIVVVNL TQCMSGKVNM
0281 GGYATGNALA HAGVIGGADM TVEATLTKLH YLLSQELDTE TIRKAMSQNL RGELTPDD
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GCTGTTTCGC CCCATATTTC CTTATCCGCA
-070 TAGTATCAGG TGCGCTCCCC CTGCCTCACG TATATACTTT TGCTCTTTCG ATATCATTCA TATCAATATC
0001 atgcaaaaga aatcaattta cgttgcctac acgggcggga ccatcgggat gcagcgttcc gagcagggtt
0071 atataccggt gtcaggtcat ctacaacgcc aactggcgct gatgccggaa ttccatcgcc cggagatgcc
0141 agatttcacc attcatgaat atacgccgct gatggattct tcagatatga cgccggaaga ctggcagcat
0211 attgctgaag atattaaagc gcactatgac gactatgatg gttttgtcat tctgcacggc accgacacga
0281 tggcgtatac cgcctctgcg ctgtcgttca tgctcgagaa tctcggtaaa ccggtcattg tgacagggtc
0351 acaaatcccg ctggctgagt tacgctctga cggacaaatt aatctgctga atgcgttgta cgttgcggcg
0421 aattatccga tcaacgaagt aacgctcttt ttcaataacc gattgtatcg cggcaaccgc actaccaaag
0491 cccatgccga tggttttgat gcgtttgcct ctccaaacct tcctccgtta ctggaagcag gtatccatat
0561 tcgtcgtttg aatacgccac ccgccccgca cggtgaaggg gaattaatcg ttcatccaat caccccacaa
0631 ccaattggcg tagtgacgat ttatccaggg atttctgctg acgtcgtgcg caattttctg cgccaaccgg
0701 tgaaagcatt gattctgcgc tcctatggcg tgggtaatgc gccacaaaac aaagccttcc tgcaggaatt
0771 acaagaagcc agcgatcgcg gtattgtggt ggtcaacctg acacaatgta tgtccggtaa agtgaacatg
0841 ggtggttatg ccaccggtaa cgccctcgcc catgccggcg taattggcgg tgcagatatg actgtagaag
0911 ccacgctaac caaactgcat tacctgctta gccaggaact ggatactgaa accattcgca aggccatgag
0981 ccaaaacctg cgcggcgaac tgacgccgga tgattaaGGA GACTGTAATG CCCCCTCGCG CCCTGTTACT
1051 GGTCGATTTA CAAAATGATT TCTGTGCTGG TGGCGCGCTC GCCGTGCCGG AAGGTGACAG TACGGTG
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