MG1655
W3110
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Gene Report : spoT
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
J Biol Chem. 1993;268(19):14316-21.
Guanylate kinase of Escherichia coli K-12.
Gentry D, Bengra C, Ikehara K, Cashel M. ( 8390989 )
     Deletion OCL07 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  spoT
Related strains
     Strains Search Search strains by gene name:  spoT   Search strains by all related name:  spoT b3650 ECK3640 JW3625 o702

General information  (Go to Linear View:)
 Gene Name spoT  
 Alternative name b3650,ECK3640,JW3625,o702  
 Location, Length 3,820,423 - 3,822,531 (  +  ) ;   82.34 min ; 2109 (bp) ,   702 (aa) Go to Linear View
 Product bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase  
 Operon Name rpoZ-spoT-trmH-recG  
 Note (p)ppGpp synthetase II; also guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0008233 - peptidase activity; GO_process: GO:0015949 - nucleobase, nucleoside and nucleotide interconversion; GO_process: GO:0042594 - response to starvation  
 Function enzyme; Global regulatory functions  
 Gene Ontology GO:0003824 ; catalytic activity ( spoT )
GO:0008893 ; guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity ( spoT )
GO:0015969 ; guanosine tetraphosphate metabolic process ( spoT )
GO:0016787 ; hydrolase activity ( spoT )
GO:0030145 ; manganese ion binding ( spoT )
 PID 1790082  
 EC number
  (KEGG Pathway)   		2.7.6.5   3.1.7.2  
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) SPOT_ECOLI ( P17580 )
    -  Protein name Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase  
    -  Synonyms EC 3.1.7.2) ((ppGpp)ase, Penta-phosphate guanosine-3'-pyrophosphohydrolase  
    -  Gene name Name=spoT; OrderedLocusNames=b3650, z5076, ECs4525, SF3690, S4079;  

 Linear View (Whole Mode)
View Location
   3810.0  –  3835.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP spoT (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of The Bifunctional Catalytic Fragment Of Relseq, The RelaSPOT HOMOLOG FROM STREPTOCOCCUS Equisimilis.  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase ((ppGpp)ase) (Penta-phosphate guanosine-3'-pyrophosphohydrolase)  
         PSI-BLAST GTP pyrophosphokinase
    nr     (database updated : 2007.02.20 )
         BLAST (p)ppGpp synthetase II; also guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AMIDATION    PROKAR_LIPOPROTEIN     

Other Cross-Reference
    COG COG0317TK 
    EcoCyc spoT 

MMBR References
J Biol Chem. 1989;264:9122-9125
Protein sequences encoded by the relA and the spoT genes of Escherichia coli are interrelated.
Metzger, S., E. Sarubbi, G. Glaser, and M. Cashel.
J Mol Biol. 1996;259(1):41-57.
Control of spoT-dependent ppGpp synthesis and degradation in Escherichia coli.
Murray KD, Bremer H. ( 8648647 )
J Biol Chem. 1989;264:15074-15082
Characterization of the spoT gene of Escherichia coli.
Sarubbi, E., K. E. Rudd, H. Xiao, K. Ikehara, M. Kalman, and M. Cashel.
Mol Gen Genet. 1988;213(2-3):214-22.
Basal ppGpp level adjustment shown by new spoT mutants affect steady state growth rates and rrnA ribosomal promoter regulation in Escherichia coli.
Sarubbi E, Rudd KE, Cashel M. ( 2460731 )
J Biol Chem. 1991;266:5980-5990
Residual guanosine 3',5'-bispyrophosphate synthetic activity of relA null mutants can be eliminated by spoT null mutations.
Xiao, H., M. Kalman, K. Ikehara, S. Zemel, G. Glaser, and M. Cashel.

Sequences
Amino acid
FASTA format 
0001 MYLFESLNQL IQTYLPEDQI KRLRQAYLVA RDAHEGQTRS SGEPYITHPV AVACILAEMK LDYETLMAAL 
0071 LHDVIEDTPA TYQDMEQLFG KSVAELVEGV SKLDKLKFRD KKEAQAENFR KMIMAMVQDI RVILIKLADR 
0141 THNMRTLGSL RPDKRRRIAR ETLEIYSPLA HRLGIHHIKT ELEELGFEAL YPNRYRVIKE VVKAARGNRK 
0211 EMIQKILSEI EGRLQEAGIP CRVSGREKHL YSIYCKMVLK EQRFHSIMDI YAFRVIVNDS DTCYRVLGQM 
0281 HSLYKPRPGR VKDYIAIPKA NGYQSLHTSM IGPHGVPVEV QIRTEDMDQM AEMGVAAHWA YKEHGETSTT 
0351 AQIRAQRWMQ SLLELQQSAG SSFEFIESVK SDLFPDEIYV FTPEGRIVEL PAGATPVDFA YAVHTDIGHA 
0421 CVGARVDRQP YPLSQPLTSG QTVEIITAPG ARPNAAWLNF VVSSKARAKI RQLLKNLKRD DSVSLGRRLL 
0491 NHALGGSRKL NEIPQENIQR ELDRMKLATL DDLLAEIGLG NAMSVVVAKN LQHGDASIPP ATQSHGHLPI 
0561 KGADGVLITF AKCCRPIPGD PIIAHVSPGK GLVIHHESCR NIRGYQKEPE KFMAVEWDKE TAQEFITEIK 
0631 VEMFNHQGAL ANLTAAINTT TSNIQSLNTE EKDGRVYSAF IRLTARDRVH LANIMRKIRV MPDVIKVTRN 
0701 RN
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             CGTTCGCGAA CGCCAGGAAC AGCAAGAGCA 
-070 GGAAGCCGCT GAATTACAAG CCGTTACCGC TATTGCTGAA GGTCGTCGTT AATCACAAAG CGGGTCGCCC 
0001 ttgtatctgt ttgaaagcct gaatcaactg attcaaacct acctgccgga agaccaaatc aagcgtctgc 
0071 ggcaggcgta tctcgttgca cgtgatgctc acgaggggca aacacgttca agcggtgaac cctatatcac 
0141 gcacccggta gcggttgcct gcattctggc cgagatgaaa ctcgactatg aaacgctgat ggcggcgctg 
0211 ctgcatgacg tgattgaaga tactcccgcc acctaccagg atatggaaca gctttttggt aaaagcgtcg 
0281 ccgagctggt agagggggtg tcgaaacttg ataaactcaa gttccgcgat aagaaagagg cgcaggccga 
0351 aaactttcgc aagatgatta tggcgatggt gcaggatatc cgcgtcatcc tcatcaaact tgccgaccgt 
0421 acccacaaca tgcgcacgct gggctcactt cgcccggaca aacgtcgccg catcgcccgt gaaactctcg 
0491 aaatttatag cccgctggcg caccgtttag gtatccacca cattaaaacc gaactcgaag agctgggttt 
0561 tgaggcgctg tatcccaacc gttatcgcgt aatcaaagaa gtggtgaaag ccgcgcgcgg caaccgtaaa 
0631 gagatgatcc agaagattct ttctgaaatc gaagggcgtt tgcaggaagc gggaataccg tgccgcgtca 
0701 gtggtcgcga gaagcatctt tattcgattt actgcaaaat ggtgctcaaa gagcagcgtt ttcactcgat 
0771 catggacatc tacgctttcc gcgtgatcgt caatgattct gacacctgtt atcgcgtgct gggccagatg 
0841 cacagcctgt acaagccgcg tccgggccgc gtgaaagact atatcgccat tccaaaagcg aacggctatc 
0911 agtctttgca cacctcgatg atcggcccgc acggtgtgcc ggttgaggtc cagatccgta ccgaagatat 
0981 ggaccagatg gcggagatgg gtgttgccgc gcactgggct tataaagagc acggcgaaac cagtactacc 
1051 gcacaaatcc gcgcccagcg ctggatgcaa agcctgctgg agctgcaaca gagcgccggt agttcgtttg 
1121 aatttatcga gagcgttaaa tccgatctct tcccggatga gatttacgtt ttcacaccgg aagggcgcat 
1191 tgtcgagctg cctgccggtg caacgcccgt cgacttcgct tatgcagtgc ataccgatat cggtcatgcc 
1261 tgcgtgggcg cacgcgttga ccgccagcct tacccgctgt cgcagccgct taccagcggt caaaccgttg 
1331 aaatcattac cgctccgggc gctcgcccga atgccgcttg gctgaacttt gtcgttagct cgaaagcgcg 
1401 cgccaaaatt cgtcagttgc tgaaaaacct caagcgtgat gattctgtaa gcctgggccg tcgtctgctc 
1471 aaccatgctt tgggtggtag ccgtaagctg aatgaaatcc cgcaggaaaa tattcagcgc gagctggatc 
1541 gcatgaagct ggcaacgctt gacgatctgc tggcagaaat cggacttggt aacgcaatga gcgtggtggt 
1611 cgcgaaaaat ctgcaacatg gggacgcctc cattccaccg gcaacccaaa gccacggaca tctgcccatt 
1681 aaaggtgccg atggcgtgct gatcaccttt gcgaaatgct gccgccctat tcctggcgac ccgattatcg 
1751 cccacgtcag ccccggtaaa ggtctggtga tccaccatga atcctgccgt aatatccgtg gctaccagaa 
1821 agagccagag aagtttatgg ctgtggaatg ggataaagag acggcgcagg agttcatcac cgaaatcaag 
1891 gtggagatgt tcaatcatca gggtgcgctg gcaaacctga cggcggcaat taacaccacg acttcgaata 
1961 ttcaaagttt gaatacggaa gagaaagatg gtcgcgtcta cagcgccttt attcgtctga ccgctcgtga 
2031 ccgtgtgcat ctggcgaata tcatgcgcaa aatccgcgtg atgccagacg tgattaaagt cacccgaaac 
2101 cgaaattaaT GTTTTATGAA CCCAACACGT TATGCACGCA TCTGCGAAAT GCTCGCCAGG CGGCAGCCTG 
2171 ATCTGACCGT CTGCATGGAG CAGGTCCACA AACCTCATA