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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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thrA34::Tn10
,
OCR27
(D)
,
OCR27-1
(D)
,
OCR27-6
(D)
,
OCR27-7
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
thrA
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Related strains
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Strains Search
|
Search strains by gene name:
thrA
Search strains by all related name:
thrA b0002 ECK0002 Hs JW0001 thrA1 thrA2 thrD
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General information (Go to Linear View: )
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Gene Name
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thrA
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Alternative name
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b0002,ECK0002,Hs,JW0001,thrA1,thrA2,thrD
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Location, Length
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337 - 2,799
(
+
)
;
0.01
min
;
2463
(bp) ,
820
(aa)
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Product
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fused aspartokinase I and homoserine dehydrogenase I
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Operon Name
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thrLABC
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Note
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bifunctional: aspartokinase I (N-terminal); homoserine dehydrogenase I (C-terminal); GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009088 - threonine biosynthetic process; GO_process: GO:0009086 - methionine biosynthetic process; GO_process: GO:0009090 - homoserine biosynthetic process
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Function
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enzyme; Amino acid biosynthesis: Threonine,1.5.1.8 metabolism; building block biosynthesis; amino acids; threonine,1.5.1.21 metabolism; building block biosynthesis; amino acids; homoserine,7.1 location of gene products; cytoplasm
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Gene Ontology
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GO:0003824
;
catalytic activity ( thrA )
GO:0004072
;
aspartate kinase activity ( thrA )
GO:0004412
;
homoserine dehydrogenase activity ( thrA )
GO:0005488
;
binding ( thrA )
GO:0008152
;
metabolic process ( thrA )
GO:0008652
;
amino acid biosynthetic process ( thrA )
GO:0009067
;
aspartate family amino acid biosynthetic process ( thrA )
GO:0009088
;
threonine biosynthetic process ( thrA )
GO:0016301
;
kinase activity ( thrA )
GO:0016491
;
oxidoreductase activity ( thrA )
GO:0016597
;
amino acid binding ( thrA )
GO:0016740
;
transferase activity ( thrA )
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PID
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1786183
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EC number
(KEGG Pathway)
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1.1.1.3
2.7.2.4
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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thrA
(
EG10998
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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AK1H_ECOLI
(
P00561
;
Q47659
)
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- Protein name
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Bifunctional aspartokinase/homoserine dehydrogenase I
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- Synonyms
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AKI-HDI
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- Gene name
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Name=thrA; Synonyms=thrA1, thrA2; OrderedLocusNames=b0002;
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MMBR References
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J Bacteriol. 1979;140(1):106-13.
Structural and functional analysis of cloned deoxyribonucleic acid containing the trpR-thr region of the Escherichia coli chromosome.
Gunsalus RP, Zurawski G, Yanofsky C.
(
159284
)
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Proc Natl Acad Sci U S A. 1980;77:5730-5733
Nucleotide sequence of the thrA gene of Escherichia coli.
Katinka, M., P. Cossart, L. Sibilli, I. Saint-Girons, M. A. Chalvignac, G. LeBras, G. N. Cohen, and M. Yaniv.
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J Mol Biol. 1985;183(4):529-41.
Identification and characterization of mutants affecting transcription termination at the threonine operon attenuator.
Lynn SP, Bauer CE, Chapman K, Gardner JF.
(
2410621
)
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J Biol Chem. 1995;270:23330-23336
Transcription termination at the thr attenuator. Evidence that the adenine residues upstream of the stem and loop structure are not required for termination.
Yang, M. T., H. B. N. Scott, and J. F. Gardner.
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Nucleic Acids Res. 1981;9:339-347
Nucleotide sequence of the thrB gene of E. coli, and its two adjacent regions; the thrAB and thrBC junctions.
Cossart, P., M. Katinka, and M. Yaniv.
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Mol Gen Genet. 1982;188(2):225-7.
Attenuation in the threonine operon: effects of amino acids present in the presumed leader peptide in addition to threonine and isoleucine.
Daniel J, Saint-Girons I.
(
6818426
)
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| Sequences |
Amino acid
FASTA format
|
0001 MRVLKFGGTS VANAERFLRV ADILESNARQ GQVATVLSAP AKITNHLVAM IEKTISGQDA LPNISDAERI
0071 FAELLTGLAA AQPGFPLAQL KTFVDQEFAQ IKHVLHGISL LGQCPDSINA ALICRGEKMS IAIMAGVLEA
0141 RGHNVTVIDP VEKLLAVGHY LESTVDIAES TRRIAASRIP ADHMVLMAGF TAGNEKGELV VLGRNGSDYS
0211 AAVLAACLRA DCCEIWTDVD GVYTCDPRQV PDARLLKSMS YQEAMELSYF GAKVLHPRTI TPIAQFQIPC
0281 LIKNTGNPQA PGTLIGASRD EDELPVKGIS NLNNMAMFSV SGPGMKGMVG MAARVFAAMS RARISVVLIT
0351 QSSSEYSISF CVPQSDCVRA ERAMQEEFYL ELKEGLLEPL AVTERLAIIS VVGDGMRTLR GISAKFFAAL
0421 ARANINIVAI AQGSSERSIS VVVNNDDATT GVRVTHQMLF NTDQVIEVFV IGVGGVGGAL LEQLKRQQSW
0491 LKNKHIDLRV CGVANSKALL TNVHGLNLEN WQEELAQAKE PFNLGRLIRL VKEYHLLNPV IVDCTSSQAV
0561 ADQYADFLRE GFHVVTPNKK ANTSSMDYYH QLRYAAEKSR RKFLYDTNVG AGLPVIENLQ NLLNAGDELM
0631 KFSGILSGSL SYIFGKLDEG MSFSEATTLA REMGYTEPDP RDDLSGMDVA RKLLILARET GRELELADIE
0701 IEPVLPAEFN AEGDVAAFMA NLSQLDDLFA ARVAKARDEG KVLRYVGNID EDGVCRVKIA EVDGNDPLFK
0771 VKNGENALAF YSHYYQPLPL VLRGYGAGND VTAAGVFADL LRTLSWKLGV
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 AGGTAACGGT GCGGGCTGAC GCGTACAGGA
-070 AACACAGAAA AAAGCCCGCA CCTGACAGTG CGGGCTTTTT TTTTCGACCA AAGGTAACGA GGTAACAACC
0001 atgcgagtgt tgaagttcgg cggtacatca gtggcaaatg cagaacgttt tctgcgtgtt gccgatattc
0071 tggaaagcaa tgccaggcag gggcaggtgg ccaccgtcct ctctgccccc gccaaaatca ccaaccacct
0141 ggtggcgatg attgaaaaaa ccattagcgg ccaggatgct ttacccaata tcagcgatgc cgaacgtatt
0211 tttgccgaac ttttgacggg actcgccgcc gcccagccgg ggttcccgct ggcgcaattg aaaactttcg
0281 tcgatcagga atttgcccaa ataaaacatg tcctgcatgg cattagtttg ttggggcagt gcccggatag
0351 catcaacgct gcgctgattt gccgtggcga gaaaatgtcg atcgccatta tggccggcgt attagaagcg
0421 cgcggtcaca acgttactgt tatcgatccg gtcgaaaaac tgctggcagt ggggcattac ctcgaatcta
0491 ccgtcgatat tgctgagtcc acccgccgta ttgcggcaag ccgcattccg gctgatcaca tggtgctgat
0561 ggcaggtttc accgccggta atgaaaaagg cgaactggtg gtgcttggac gcaacggttc cgactactct
0631 gctgcggtgc tggctgcctg tttacgcgcc gattgttgcg agatttggac ggacgttgac ggggtctata
0701 cctgcgaccc gcgtcaggtg cccgatgcga ggttgttgaa gtcgatgtcc taccaggaag cgatggagct
0771 ttcctacttc ggcgctaaag ttcttcaccc ccgcaccatt acccccatcg cccagttcca gatcccttgc
0841 ctgattaaaa ataccggaaa tcctcaagca ccaggtacgc tcattggtgc cagccgtgat gaagacgaat
0911 taccggtcaa gggcatttcc aatctgaata acatggcaat gttcagcgtt tctggtccgg ggatgaaagg
0981 gatggtcggc atggcggcgc gcgtctttgc agcgatgtca cgcgcccgta tttccgtggt gctgattacg
1051 caatcatctt ccgaatacag catcagtttc tgcgttccac aaagcgactg tgtgcgagct gaacgggcaa
1121 tgcaggaaga gttctacctg gaactgaaag aaggcttact ggagccgctg gcagtgacgg aacggctggc
1191 cattatctcg gtggtaggtg atggtatgcg caccttgcgt gggatctcgg cgaaattctt tgccgcactg
1261 gcccgcgcca atatcaacat tgtcgccatt gctcagggat cttctgaacg ctcaatctct gtcgtggtaa
1331 ataacgatga tgcgaccact ggcgtgcgcg ttactcatca gatgctgttc aataccgatc aggttatcga
1401 agtgtttgtg attggcgtcg gtggcgttgg cggtgcgctg ctggagcaac tgaagcgtca gcaaagctgg
1471 ctgaagaata aacatatcga cttacgtgtc tgcggtgttg ccaactcgaa ggctctgctc accaatgtac
1541 atggccttaa tctggaaaac tggcaggaag aactggcgca agccaaagag ccgtttaatc tcgggcgctt
1611 aattcgcctc gtgaaagaat atcatctgct gaacccggtc attgttgact gcacttccag ccaggcagtg
1681 gcggatcaat atgccgactt cctgcgcgaa ggtttccacg ttgtcacgcc gaacaaaaag gccaacacct
1751 cgtcgatgga ttactaccat cagttgcgtt atgcggcgga aaaatcgcgg cgtaaattcc tctatgacac
1821 caacgttggg gctggattac cggttattga gaacctgcaa aatctgctca atgcaggtga tgaattgatg
1891 aagttctccg gcattctttc tggttcgctt tcttatatct tcggcaagtt agacgaaggc atgagtttct
1961 ccgaggcgac cacgctggcg cgggaaatgg gttataccga accggacccg cgagatgatc tttctggtat
2031 ggatgtggcg cgtaaactat tgattctcgc tcgtgaaacg ggacgtgaac tggagctggc ggatattgaa
2101 attgaacctg tgctgcccgc agagtttaac gccgagggtg atgttgccgc ttttatggcg aatctgtcac
2171 aactcgacga tctctttgcc gcgcgcgtgg cgaaggcccg tgatgaagga aaagttttgc gctatgttgg
2241 caatattgat gaagatggcg tctgccgcgt gaagattgcc gaagtggatg gtaatgatcc gctgttcaaa
2311 gtgaaaaatg gcgaaaacgc cctggccttc tatagccact attatcagcc gctgccgttg gtactgcgcg
2381 gatatggtgc gggcaatgac gttacagctg ccggtgtctt tgctgatctg ctacgtaccc tctcatggaa
2451 gttaggagtc tgaCATGGTT AAAGTTTATG CCCCGGCTTC CAGTGCCAAT ATGAGCGTCG GGTTTGATGT
2521 GCTCGGGGCG GCGGTGACAC CTGTTGATGG TGCATTGCTC GGA
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