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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
|
|
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Deletion
|
DE(xthA-pncA)90
,
LD3-17-1
(LD)
,
OCL84,85-1
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
gdhA
|
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Related strains
|
|
Strains Search
|
Search strains by gene name:
gdhA
Search strains by all related name:
gdhA b1761 ECK1759 JW1750
|
General information (Go to Linear View: )
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Gene Name
|
gdhA
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|
Alternative name
|
b1761,ECK1759,JW1750
|
|
Location, Length
|
1,840,395 - 1,841,738
(
+
)
;
39.67
min
;
1344
(bp) ,
447
(aa)
|
|
Product
|
glutamate dehydrogenase, NADP-specific
|
|
Operon Name
|
gdhA
|
|
Note
|
NADP-specific glutamate dehydrogenase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006537 - glutamate biosynthetic process
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Function
|
enzyme; Amino acid biosynthesis: Glutamate
|
|
Gene Ontology
|
GO:0003824
;
catalytic activity ( gdhA )
GO:0004354
;
glutamate dehydrogenase (NADP+) activity ( gdhA )
GO:0005488
;
binding ( gdhA )
GO:0006520
;
amino acid metabolic process ( gdhA )
GO:0008152
;
metabolic process ( gdhA )
GO:0016491
;
oxidoreductase activity ( gdhA )
|
|
PID
|
1788059
|
EC number
(KEGG Pathway)
|
1.4.1.4
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
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EcoGene
|
gdhA
(
EG10372
)
|
|
Number of removed
|
0 aa cleaved
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
DHE4_ECOLI
(
P00370
;
P78173
)
|
|
- Protein name
|
NADP-specific glutamate dehydrogenase
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- Synonyms
|
EC 1.4.1.4, NADP-GDH
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- Gene name
|
Name=gdhA; OrderedLocusNames=b1761;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
|
gdhA
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Glutamate Dehydrogenase
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
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BLAST
|
NADP-specific glutamate dehydrogenase
|
|
PSI-BLAST
|
NADP-specific glutamate dehydrogenase
|
|
nr
(database updated :
2007.02.20
)
|
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BLAST
|
glutamate dehydrogenase [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
|
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PROSITE
|
ASN_GLYCOSYLATION
GLYCOSAMINOGLYCAN
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
GLFV_DEHYDROGENASE
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MMBR References
|
Mol Gen Genet. 1990;223(3):508-12.
The glutamate dehydrogenase structural gene of Escherichia coli.
Helling RB.
(
2270089
)
|
Gene. 1985;37(1-3):53-62.
Repetitive extragenic palindromic (REP) sequences in the Escherichia coli gdhA gene.
Becerril B, Valle F, Merino E, Riba L, Bolivar F.
(
3902576
)
|
Nucleic Acids Res. 1983;11:5257-5266
A complete nucleotide sequence of the Escherichia coli gdhA gene.
McPherson, M. J., and J. C. Wootton.
|
Gene. 1982;17(1):1-8.
Recombinant plasmids carrying the glutamate dehydrogenase structural gene from Escherichia coli K-12.
Sanchez-Pescador R, Sanvicente E, Valle F, Bolivar F.
(
7042463
)
|
Gene. 1984;27(2):193-9.
Complete nucleotide sequence of the glutamate dehydrogenase gene from Escherichia coli K-12.
Valle F, Becerril B, Chen E, Seeburg P, Heyneker H, Bolivar F.
(
6373501
)
|
Gene. 1983;23(2):199-209.
Nucleotide sequence of the promoter and amino-terminal coding region of the glutamate dehydrogenase structural gene of Escherichia coli.
Valle F, Sanvicente E, Seeburg P, Covarrubias A, Rodriguez RL, Bolivar F.
(
6225701
)
|
Plasmid. 1980;3(2):150-64.
ColE1 hybrid plasmids containing Escherichia coli genes involved in the biosynthesis of glutamate and glutamine.
Covarrubias AA, Sanchez-Pescador R, Osorio A, Bolivar F, Bastarrachea F.
(
6152835
)
|
Biotechnol Appl Biochem. 1993;18 (Pt 2):139-42.
Isolation and cloning of the gene from Escherichia coli encoding glutamate decarboxylase.
De Biase D, Simmaco M, Sweeney G, Barra D, Bossa F, John RA.
(
8251111
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MDQTYSLESF LNHVQKRDPN QTEFAQAVRE VMTTLWPFLE QNPKYRQMSL LERLVEPERV IQFRVVWVDD
0071 RNQIQVNRAW RVQFSSAIGP YKGGMRFHPS VNLSILKFLG FEQTFKNALT TLPMGGGKGG SDFDPKGKSE
0141 GEVMRFCQAL MTELYRHLGA DTDVPAGDIG VGGREVGFMA GMMKKLSNNT ACVFTGKGLS FGGSLIRPEA
0211 TGYGLVYFTE AMLKRHGMGF EGMRVSVSGS GNVAQYAIEK AMEFGARVIT ASDSSGTVVD ESGFTKEKLA
0281 RLIEIKASRD GRVADYAKEF GLVYLEGQQP WSLPVDIALP CATQNELDVD AAHQLIANGV KAVAEGANMP
0351 TTIEATELFQ QAGVLFAPGK AANAGGVATS GLEMAQNAAR LGWKAEKVDA RLHHIMLDIH HACVEHGGEG
0421 EQTNYVQGAN IAGFVKVADA MLAQGVI
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GCTTACCGTC ACATTCTTGA TGGTATAGTC
-070 GAAAACTGCA AAAGCACATG ACATAAACAA CATAAGCACA ATCGTATTAA TATATAAGGG TTTTATATCT
0001 atggatcaga catattctct ggagtcattc ctcaaccatg tccaaaagcg cgacccgaat caaaccgagt
0071 tcgcgcaagc cgttcgtgaa gtaatgacca cactctggcc ttttcttgaa caaaatccaa aatatcgcca
0141 gatgtcatta ctggagcgtc tggttgaacc ggagcgcgtg atccagtttc gcgtggtatg ggttgatgat
0211 cgcaaccaga tacaggtcaa ccgtgcatgg cgtgtgcagt tcagctctgc catcggcccg tacaaaggcg
0281 gtatgcgctt ccatccgtca gttaaccttt ccattctcaa attcctcggc tttgaacaaa ccttcaaaaa
0351 tgccctgact actctgccga tgggcggtgg taaaggcggc agcgatttcg atccgaaagg aaaaagcgaa
0421 ggtgaagtga tgcgtttttg ccaggcgctg atgactgaac tgtatcgcca cctgggcgcg gataccgacg
0491 ttccggcagg tgatatcggg gttggtggtc gtgaagtcgg ctttatggcg gggatgatga aaaagctctc
0561 caacaatacc gcctgcgtct tcaccggtaa gggcctttca tttggcggca gtcttattcg cccggaagct
0631 accggctacg gtctggttta tttcacagaa gcaatgctaa aacgccacgg tatgggtttt gaagggatgc
0701 gcgtttccgt ttctggctcc ggcaacgtcg cccagtacgc tatcgaaaaa gcgatggaat ttggtgctcg
0771 tgtgatcact gcgtcagact ccagcggcac tgtagttgat gaaagcggat tcacgaaaga gaaactggca
0841 cgtcttatcg aaatcaaagc cagccgcgat ggtcgagtgg cagattacgc caaagaattt ggtctggtct
0911 atctcgaagg ccaacagccg tggtctctac cggttgatat cgccctgcct tgcgccaccc agaatgaact
0981 ggatgttgac gccgcgcatc agcttatcgc taatggcgtt aaagccgtcg ccgaaggggc aaatatgccg
1051 accaccatcg aagcgactga actgttccag caggcaggcg tactatttgc accgggtaaa gcggctaatg
1121 ctggtggcgt cgctacatcg ggcctggaaa tggcacaaaa cgctgcgcgc ctgggctgga aagccgagaa
1191 agttgacgca cgtttgcatc acatcatgct ggatatccac catgcctgtg ttgagcatgg tggtgaaggt
1261 gagcaaacca actacgtgca gggcgcgaac attgccggtt ttgtgaaggt tgccgatgcg atgctggcgc
1331 agggtgtgat ttaaGTTGTA AATGCCTGAT GGCGCTACGC TTATCAGGCC TACAAATGGG CACAATTCAT
1401 TGCAGTTACG CTCTAATGTA GGCCGGGCAA GCGCAGCGCC CCCG
|
