|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Mol Gen Genet. 1983;189(2):215-21.
Mutants of Escherichia coli defective in penicillin-insensitive murein DD-endopeptidase.
Iida K, Hirota Y, Schwarz U.
(
6343788
)
|
|
|
Deletion
|
OCL64
(D)
,
OCL64-4
(D)
,
OCL64-7
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
mepA
|
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Related strains
|
|
Strains Search
|
Search strains by gene name:
mepA
Search strains by all related name:
mepA b2328 ECK2322 JW2325
|
General information (Go to Linear View: )
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|
Gene Name
|
mepA
|
|
Alternative name
|
b2328,ECK2322,JW2325
|
|
Location, Length
|
2,443,582 - 2,444,406
(
-
)
;
52.67
min
;
825
(bp) ,
274
(aa)
|
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Product
|
murein DD-endopeptidase
|
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Operon Name
|
prmB-aroC-mepA-yfcAML
|
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Note
|
murein DD-endopeptidase, penicillin-insensitive; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_process: GO:0009252 - peptidoglycan biosynthetic process; GO_process: GO:0042493 - response to drug
|
|
Function
|
enzyme; Murein sacculus, peptidoglycan
|
|
Gene Ontology
|
GO:0000270
;
peptidoglycan metabolic process ( mepA )
GO:0004222
;
metalloendopeptidase activity ( mepA )
GO:0006508
;
proteolysis ( mepA )
GO:0008233
;
peptidase activity ( mepA )
GO:0008237
;
metallopeptidase activity ( mepA )
GO:0008270
;
zinc ion binding ( mepA )
GO:0008931
;
murein DD-endopeptidase activity ( mepA )
GO:0016787
;
hydrolase activity ( mepA )
GO:0030288
;
outer membrane-bounded periplasmic space ( mepA )
GO:0042597
;
periplasmic space ( mepA )
GO:0046872
;
metal ion binding ( mepA )
|
|
PID
|
1788668
|
EC number
(KEGG Pathway)
|
3.4.24.-
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
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EcoGene
|
mepA
(
EG10580
)
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Number of removed
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19 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
MEPA_ECOLI
(
P14007
)
|
|
- Protein name
|
Penicillin-insensitive murein endopeptidase precursor
|
|
- Synonyms
|
EC 3.4.99.-, D-alanyl-D-alanine-endopeptidase, DD-endopeptidase
|
|
- Gene name
|
Name=mepA; OrderedLocusNames=b2328, SF2404, S2539;
|
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MMBR References
|
Mol Gen Genet. 1983;189(2):215-21.
Mutants of Escherichia coli defective in penicillin-insensitive murein DD-endopeptidase.
Iida K, Hirota Y, Schwarz U.
(
6343788
)
|
Mol Microbiol. 1990;4(2):209-19.
Cloning and characterization of mepA, the structural gene of the penicillin-insensitive murein endopeptidase from Escherichia coli.
Keck W, van Leeuwen AM, Huber M, Goodell EW.
(
2187143
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MNKTAIALLA LLASSASLAA TPWQKITQPV PGSAQSIGSF SNGCIVGADT LPIQSEHYQV MRTDQRRYFG
0071 HPDLVMFIQR LSSQVSNLGM GTVLIGDMGM PAGGRFNGGH ASHQTGLDVD IFLQLPKTRW TSAQLLRPQA
0141 LDLVSRDGKH VVSTLWKPEI FSLIKLAAQD KDVTRIFVNP AIKQQLCLDA GTDRDWLRKV RPWFQHRAHM
0211 HVRLRCPADS LECEDQPLPP SGDGCGAELQ SWFEPPKPGT TKPEKKTPPP LPPSCQALLD EHVI
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gatcgcagaa gcgatgctgg cgatcgtttt
-070 aatggatcac ctgttacggc aacgggcgca aaatgccgat gtgaagactg atattccacg ctggtaaaaa
0001 atgaataaaa ccgcgattgc gctgctggct ctgcttgcca gtagcgccag cctggcagcg acgccgtggc
0071 aaaaaataac ccaacctgtg ccgggtagcg cacaatcgat aggcagtttt tctaatggct gtattgtcgg
0141 cgctgacacg ctgccgatac agtccgaaca ttatcaggtc atgcgtaccg atcagcgtcg ctatttcggt
0211 cacccggatc tggtgatgtt tatccagcgt ctgagtagcc aggtgagcaa tctgggcatg ggtacggtgc
0281 tgattggcga tatggggatg cccgctggtg ggcgtttcaa cggcggtcat gccagccacc agaccggact
0351 ggatgtcgat atctttctgc aactgccgaa aactcgctgg acctccgcgc agctcttgcg cccgcaagca
0421 ctggacttag tttcccgcga cggtaaacac gttgtctcca cgctgtggaa gccagaaatt ttcagcttga
0491 tcaaactcgc cgcccaggac aaagacgtca cgcgcatttt tgttaatccg gcgattaaac aacaactttg
0561 ccttgatgcg ggcaccgatc gcgactggtt gcgcaaagtg cgaccctggt tccagcatcg cgcgcatatg
0631 catgtacgat tacgttgccc tgccgatagt ctggagtgtg aagatcaacc tttaccgcca tcaggcgatg
0701 gttgcggggc agaactgcaa agctggtttg aacctccaaa accgggaaca acaaagcctg agaagaagac
0771 accgcctccg ttgccgcctt cctgccaggc gctactggat gagcacgtga tctaatggaa acgtttaata
0841 gcctgtttat ggtttccccg ctgttgctgg gagttctctt ttttgtcgcc atgctggcgg gatttatcga
0911 ctcgattgcc ggtgg
|
